+ mkdir aih-tih-sc-12b72b-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-12b72b-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai 152380it [00:00, 1523641.62it/s] 320776it [00:00, 1617898.04it/s] 488330it [00:00, 1644184.27it/s] 658193it [00:00, 1665644.21it/s] 832551it [00:00, 1693685.39it/s] 1006914it [00:00, 1710646.93it/s] 1181901it [00:00, 1723447.07it/s] 1359979it [00:00, 1741680.51it/s] 1537492it [00:00, 1751953.41it/s] 1714124it [00:01, 1756370.67it/s] 1889761it [00:01, 1753590.68it/s] 2065121it [00:01, 1741254.34it/s] 2243330it [00:01, 1753532.02it/s] 2421155it [00:01, 1760947.18it/s] 2597267it [00:01, 1619874.82it/s] 2769006it [00:01, 1647283.27it/s] 2945345it [00:01, 1680695.71it/s] 3115876it [00:01, 1687719.44it/s] 3290981it [00:01, 1706313.02it/s] 3462324it [00:02, 1684411.20it/s] 3631548it [00:02, 1686686.64it/s] 3800597it [00:02, 1644011.23it/s] 3971938it [00:02, 1664223.97it/s] 4138739it [00:02, 1645979.26it/s] 4307310it [00:02, 1657609.73it/s] 4473292it [00:02, 1650906.81it/s] 4641680it [00:02, 1660529.45it/s] 4813282it [00:02, 1676911.25it/s] 4981073it [00:02, 1630364.76it/s] 5150328it [00:03, 1648538.32it/s] 5315457it [00:03, 1615193.70it/s] 5485570it [00:03, 1640263.21it/s] 5649871it [00:03, 1577181.06it/s] 5808209it [00:03, 1559476.68it/s] 5974204it [00:03, 1588472.63it/s] 6135575it [00:03, 1595819.79it/s] 6309565it [00:03, 1638210.43it/s] 6473678it [00:03, 1595067.69it/s] 6640804it [00:03, 1617190.66it/s] 6815651it [00:04, 1655760.13it/s] 6992137it [00:04, 1688003.74it/s] 7166718it [00:04, 1705143.82it/s] 7342963it [00:04, 1722190.10it/s] 7517577it [00:04, 1729317.17it/s] 7690622it [00:04, 1719439.52it/s] 7862656it [00:04, 1703358.65it/s] 8038431it [00:04, 1719470.02it/s] 8217191it [00:04, 1739725.73it/s] 8393433it [00:05, 1746475.57it/s] 8568135it [00:05, 1724265.73it/s] 8740656it [00:05, 1709847.87it/s] 8911719it [00:05, 1695546.62it/s] 9081337it [00:05, 1677355.95it/s] 9257496it [00:05, 1702126.61it/s] 9431648it [00:05, 1713733.38it/s] 9603100it [00:05, 1694831.58it/s] 9772670it [00:05, 1684831.00it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai 9941214it [00:05, 1670964.22it/s] 10112290it [00:06, 1682667.75it/s] 10280856it [00:06, 1683523.72it/s] 10449247it [00:06, 1667907.99it/s] 10623576it [00:06, 1690220.54it/s] 10792660it [00:06, 1685548.48it/s] 10963585it [00:06, 1692497.14it/s] 11132870it [00:06, 1659359.38it/s] 11299278it [00:06, 1660727.57it/s] 11471433it [00:06, 1678720.66it/s] 11639405it [00:06, 1669166.62it/s] 11812902it [00:07, 1688680.27it/s] 11981843it [00:07, 1686064.17it/s] 12150500it [00:07, 1549420.54it/s] 12307597it [00:07, 1548727.27it/s] 12477322it [00:07, 1591149.83it/s] 12647774it [00:07, 1623964.57it/s] 12815806it [00:07, 1640440.49it/s] 12980830it [00:07, 1643311.63it/s] 13145684it [00:07, 1581727.36it/s] 13304670it [00:08, 1459474.40it/s] 13452790it [00:08, 1388681.59it/s] 13544786it [00:08, 1650262.84it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-12b72b-R1_A23WJ53LT4_1/aih-tih-sc-12b72b-R1_A23WJ53LT4_1.md -output aih-tih-sc-12b72b-R1_A23WJ53LT4_1/aih-tih-sc-12b72b-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-12b72b-R1_A23WJ53LT4_1/aih-tih-sc-12b72b-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-12b72b-R1_A23WJ53LT4_1/aih-tih-sc-12b72b-R1_A23WJ53LT4_1_full_basic_stats.tsv