[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-0419d4-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (8904089) splicing reads. 227631 splicing junctions. 175203 known splicing junctions. 52428 novel splicing junctions.
sampling 10% (17808178) splicing reads. 265275 splicing junctions. 186055 known splicing junctions. 79220 novel splicing junctions.
sampling 15% (26712267) splicing reads. 288576 splicing junctions. 191282 known splicing junctions. 97294 novel splicing junctions.
sampling 20% (35616357) splicing reads. 304218 splicing junctions. 194304 known splicing junctions. 109914 novel splicing junctions.
sampling 25% (44520446) splicing reads. 316034 splicing junctions. 196437 known splicing junctions. 119597 novel splicing junctions.
sampling 30% (53424535) splicing reads. 324931 splicing junctions. 197994 known splicing junctions. 126937 novel splicing junctions.
sampling 35% (62328624) splicing reads. 331950 splicing junctions. 199182 known splicing junctions. 132768 novel splicing junctions.
sampling 40% (71232714) splicing reads. 337539 splicing junctions. 200075 known splicing junctions. 137464 novel splicing junctions.
sampling 45% (80136803) splicing reads. 342110 splicing junctions. 200782 known splicing junctions. 141328 novel splicing junctions.
sampling 50% (89040892) splicing reads. 345918 splicing junctions. 201353 known splicing junctions. 144565 novel splicing junctions.
sampling 55% (97944981) splicing reads. 349086 splicing junctions. 201799 known splicing junctions. 147287 novel splicing junctions.
sampling 60% (106849071) splicing reads. 351674 splicing junctions. 202190 known splicing junctions. 149484 novel splicing junctions.
sampling 65% (115753160) splicing reads. 353904 splicing junctions. 202520 known splicing junctions. 151384 novel splicing junctions.
sampling 70% (124657249) splicing reads. 355713 splicing junctions. 202785 known splicing junctions. 152928 novel splicing junctions.
sampling 75% (133561338) splicing reads. 357233 splicing junctions. 203001 known splicing junctions. 154232 novel splicing junctions.
sampling 80% (142465428) splicing reads. 358509 splicing junctions. 203195 known splicing junctions. 155314 novel splicing junctions.
sampling 85% (151369517) splicing reads. 359544 splicing junctions. 203331 known splicing junctions. 156213 novel splicing junctions.
sampling 90% (160273606) splicing reads. 360359 splicing junctions. 203438 known splicing junctions. 156921 novel splicing junctions.
sampling 95% (169177695) splicing reads. 360977 splicing junctions. 203511 known splicing junctions. 157466 novel splicing junctions.
sampling 100% (178081785) splicing reads. 361346 splicing junctions. 203568 known splicing junctions. 157778 novel splicing junctions.