Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1f/a0d557773d3a9c2b740f3684069b86/aih-tih-sc-331e97-R1_A23WJ53LT4_1_1.fastp.fastq.gz
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/1f/a0d557773d3a9c2b740f3684069b86/aih-tih-sc-331e97-R1_A23WJ53LT4_1_2.fastp.fastq.gz
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/43d0eeeb57f6e794c1328833b6aa50/.command.sh
Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx
Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/43d0eeeb57f6e794c1328833b6aa50/.command.run
==> STAGING COMPLETE (6 inputs)
STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/aih-tih-sc-331e97-R1_A23WJ53LT4_1_1.fastp.fastq.gz input2/aih-tih-sc-331e97-R1_A23WJ53LT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix aih-tih-sc-331e97-R1_A23WJ53LT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:aih-tih-sc-331e97-R1_A23WJ53LT4_1 SM:aih-tih-sc-331e97-R1_A23WJ53LT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664
STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source
Jun 08 07:40:28 ..... started STAR run
Jun 08 07:40:28 ..... loading genome
Jun 08 07:40:49 ..... processing annotations GTF
Jun 08 07:40:58 ..... inserting junctions into the genome indices
Jun 08 07:42:16 ..... started mapping
Jun 08 07:42:30 ..... finished mapping
Jun 08 07:42:31 ..... started sorting BAM
Jun 08 07:42:32 ..... finished successfully