[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-00d05f-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	248867449
Known Splicing Events:	246652848
Partial Novel Splicing Events:	1472085
Novel Splicing Events:	670631
Filtered Splicing Events:	71885

Total splicing  Junctions:	395294
Known Splicing Junctions:	246607
Partial Novel Splicing Junctions:	97511
Novel Splicing Junctions:	51176

===================================================================
Create BED file ...
Create Interact file ...