[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2482ef-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	28891746
Known Splicing Events:	28598308
Partial Novel Splicing Events:	175064
Novel Splicing Events:	105380
Filtered Splicing Events:	12994

Total splicing  Junctions:	169571
Known Splicing Junctions:	151568
Partial Novel Splicing Junctions:	11784
Novel Splicing Junctions:	6219

===================================================================
Create BED file ...
Create Interact file ...