+ mkdir aih-tih-sc-965752-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-965752-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s] 151043it [00:00, 1510283.81it/s] 311497it [00:00, 1565683.38it/s] 469176it [00:00, 1570717.78it/s] 628249it [00:00, 1578586.00it/s] 788071it [00:00, 1585496.06it/s] 953930it [00:00, 1610321.43it/s] 1119545it [00:00, 1625233.60it/s] 1286673it [00:00, 1639860.80it/s] 1455004it [00:00, 1653413.68it/s] 1620346it [00:01, 1646934.75it/s] 1786797it [00:01, 1652270.44it/s] 1952030it [00:01, 1549803.45it/s] 2108273it [00:01, 1492360.43it/s] 2270212it [00:01, 1528432.54it/s] 2435066it [00:01, 1563087.62it/s] 2601564it [00:01, 1592820.40it/s] 2769551it [00:01, 1618359.90it/s] 2931930it [00:01, 1612115.00it/s] 3098478it [00:01, 1627896.00it/s] 3261554it [00:02, 1625489.45it/s] 3428696it [00:02, 1639121.40it/s] 3593324it [00:02, 1641163.27it/s] 3757547it [00:02, 1637510.90it/s] 3921374it [00:02, 1632950.58it/s] 4086962it [00:02, 1639706.03it/s] 4252370it [00:02, 1643953.87it/s] 4418640it [00:02, 1649526.14it/s] 4584480it [00:02, 1652142.67it/s] 4749711it [00:02, 1624297.41it/s] 4912255it [00:03, 1615274.39it/s] 5073863it [00:03, 1600344.93it/s] 5233967it [00:03, 1586182.80it/s] 5392642it [00:03, 1464991.43it/s] 5540875it [00:03, 1463373.48it/s] 5706348it [00:03, 1517843.65it/s] 5874286it [00:03, 1564530.54it/s] 6042999it [00:03, 1600229.69it/s] 6210515it [00:03, 1622273.48it/s] 6373588it [00:03, 1624685.76it/s] 6536485it [00:04, 1625930.01it/s] 6703218it [00:04, 1638244.28it/s] 6871187it [00:04, 1650596.34it/s] 7039119it [00:04, 1659161.03it/s] 7207106it [00:04, 1665337.98it/s] 7375548it [00:04, 1671032.73it/s] 7542707it [00:04, 1651905.56it/s] 7707985it [00:04, 1428115.32it/s] 7870663it [00:04, 1481448.28it/s] 8039141it [00:05, 1537808.18it/s] 8207014it [00:05, 1577769.62it/s] 8375621it [00:05, 1608984.26it/s] 8538528it [00:05, 1608957.89it/s] 8704477it [00:05, 1623767.60it/s] 8874424it [00:05, 1646110.59it/s] 9042342it [00:05, 1655900.96it/s] 9211904it [00:05, 1667707.42it/s] 9380468it [00:05, 1672941.05it/s] 9548990it [00:05, 1676590.90it/s] 9717236it [00:06, 1678167.83it/s] 9886300it [00:06, 1681882.27it/s] 10054583it [00:06, 1679618.50it/s] 10222612it [00:06, 1679546.68it/s] 10391424it [00:06, 1682093.60it/s] 10559667it [00:06, 1680757.44it/s] 10727766it [00:06, 1668681.94it/s] 10894669it [00:06, 1668609.45it/s] 11061555it [00:06, 1660403.60it/s] 11227619it [00:06, 1623819.13it/s] 11390977it [00:07, 1626587.64it/s] 11553764it [00:07, 1601801.78it/s] 11723098it [00:07, 1628673.76it/s] 11892457it [00:07, 1647846.51it/s] 12061284it [00:07, 1659829.12it/s] 12232245it [00:07, 1674633.11it/s] 12404865it [00:07, 1689934.73it/s] 12574520it [00:07, 1691884.90it/s] 12744247it [00:07, 1693471.44it/s] 12915749it [00:07, 1699843.62it/s] 13086567it [00:08, 1702321.13it/s] 13256818it [00:08, 1692594.27it/s] 13426103it [00:08, 1682903.07it/s] 13594547it [00:08, 1683340.94it/s] 13762900it [00:08, 1672417.13it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai 13930166it [00:08, 1667447.12it/s] 14096927it [00:08, 1658052.36it/s] 14262749it [00:08, 1655622.93it/s] 14428322it [00:08, 1645566.43it/s] 14595357it [00:08, 1652885.74it/s] 14763375it [00:09, 1660890.26it/s] 14932335it [00:09, 1669441.96it/s] 15100247it [00:09, 1672318.13it/s] 15268195it [00:09, 1674442.94it/s] 15437577it [00:09, 1680224.01it/s] 15605607it [00:09, 1679183.19it/s] 15773531it [00:09, 1661818.95it/s] 15940520it [00:09, 1664055.72it/s] 16109051it [00:09, 1670371.80it/s] 16276153it [00:09, 1670544.52it/s] 16443226it [00:10, 1663896.78it/s] 16609633it [00:10, 1662158.87it/s] 16775861it [00:10, 1647056.48it/s] 16940601it [00:10, 1636042.17it/s] 17104236it [00:10, 1555047.83it/s] 17260995it [00:10, 1558630.15it/s] 17426834it [00:10, 1587629.45it/s] 17586415it [00:10, 1590013.43it/s] 17749190it [00:10, 1601143.92it/s] 17913874it [00:11, 1614685.13it/s] 18078183it [00:11, 1623001.37it/s] 18240628it [00:11, 1615942.95it/s] 18404823it [00:11, 1623536.68it/s] 18515251it [00:11, 1628592.74it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-965752-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-965752-R1_A23WJ53LT4_1/aih-tih-sc-965752-R1_A23WJ53LT4_1.md -output aih-tih-sc-965752-R1_A23WJ53LT4_1/aih-tih-sc-965752-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-965752-R1_A23WJ53LT4_1/aih-tih-sc-965752-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-965752-R1_A23WJ53LT4_1/aih-tih-sc-965752-R1_A23WJ53LT4_1_full_basic_stats.tsv