[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-12b72b-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15101560) splicing reads. 254286 splicing junctions. 181715 known splicing junctions. 72571 novel splicing junctions.
sampling 10% (30203120) splicing reads. 301954 splicing junctions. 192172 known splicing junctions. 109782 novel splicing junctions.
sampling 15% (45304680) splicing reads. 331743 splicing junctions. 197379 known splicing junctions. 134364 novel splicing junctions.
sampling 20% (60406241) splicing reads. 352292 splicing junctions. 200511 known splicing junctions. 151781 novel splicing junctions.
sampling 25% (75507801) splicing reads. 367365 splicing junctions. 202513 known splicing junctions. 164852 novel splicing junctions.
sampling 30% (90609361) splicing reads. 379175 splicing junctions. 204112 known splicing junctions. 175063 novel splicing junctions.
sampling 35% (105710922) splicing reads. 388585 splicing junctions. 205248 known splicing junctions. 183337 novel splicing junctions.
sampling 40% (120812482) splicing reads. 395914 splicing junctions. 206132 known splicing junctions. 189782 novel splicing junctions.
sampling 45% (135914042) splicing reads. 402077 splicing junctions. 206878 known splicing junctions. 195199 novel splicing junctions.
sampling 50% (151015603) splicing reads. 407111 splicing junctions. 207447 known splicing junctions. 199664 novel splicing junctions.
sampling 55% (166117163) splicing reads. 411345 splicing junctions. 207888 known splicing junctions. 203457 novel splicing junctions.
sampling 60% (181218723) splicing reads. 414881 splicing junctions. 208281 known splicing junctions. 206600 novel splicing junctions.
sampling 65% (196320283) splicing reads. 417842 splicing junctions. 208594 known splicing junctions. 209248 novel splicing junctions.
sampling 70% (211421844) splicing reads. 420299 splicing junctions. 208836 known splicing junctions. 211463 novel splicing junctions.
sampling 75% (226523404) splicing reads. 422364 splicing junctions. 209076 known splicing junctions. 213288 novel splicing junctions.
sampling 80% (241624964) splicing reads. 424047 splicing junctions. 209250 known splicing junctions. 214797 novel splicing junctions.
sampling 85% (256726525) splicing reads. 425429 splicing junctions. 209376 known splicing junctions. 216053 novel splicing junctions.
sampling 90% (271828085) splicing reads. 426461 splicing junctions. 209475 known splicing junctions. 216986 novel splicing junctions.
sampling 95% (286929645) splicing reads. 427245 splicing junctions. 209563 known splicing junctions. 217682 novel splicing junctions.
sampling 100% (302031206) splicing reads. 427732 splicing junctions. 209610 known splicing junctions. 218122 novel splicing junctions.