+ mkdir aih-tih-sc-804b22-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-804b22-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai 141319it [00:00, 1412830.92it/s] 300727it [00:00, 1519401.50it/s] 457885it [00:00, 1542930.70it/s] 622296it [00:00, 1582736.88it/s] 785509it [00:00, 1600522.81it/s] 952073it [00:00, 1622637.10it/s] 1115665it [00:00, 1626959.44it/s] 1282104it [00:00, 1638852.87it/s] 1451339it [00:00, 1655538.22it/s] 1618847it [00:01, 1661552.99it/s] 1785003it [00:01, 1660990.86it/s] 1951262it [00:01, 1661458.41it/s] 2117408it [00:01, 1660590.00it/s] 2283468it [00:01, 1654773.92it/s] 2454606it [00:01, 1671769.09it/s] 2623741it [00:01, 1677529.28it/s] 2791501it [00:01, 1669663.13it/s] 2958480it [00:01, 1669327.65it/s] 3128354it [00:01, 1678096.84it/s] 3296173it [00:02, 1653905.64it/s] 3461644it [00:02, 1652611.17it/s] 3630254it [00:02, 1662551.94it/s] 3800338it [00:02, 1673947.82it/s] 3967773it [00:02, 1669137.04it/s] 4134716it [00:02, 1668977.90it/s] 4301635it [00:02, 1618942.14it/s] 4463856it [00:02, 1584841.51it/s] 4626969it [00:02, 1598217.10it/s] 4787058it [00:02, 1580151.72it/s] 4945270it [00:03, 1577476.76it/s] 5103150it [00:03, 1535649.80it/s] 5256986it [00:03, 1516445.48it/s] 5421794it [00:03, 1554675.12it/s] 5577517it [00:03, 1541284.73it/s] 5731824it [00:03, 1530702.79it/s] 5900412it [00:03, 1576271.51it/s] 6058218it [00:03, 1540298.60it/s] 6214957it [00:03, 1548099.95it/s] 6382560it [00:03, 1585737.59it/s] 6544771it [00:04, 1596421.79it/s] 6713002it [00:04, 1621920.96it/s] 6880580it [00:04, 1637950.45it/s] 7045439it [00:04, 1640937.71it/s] 7211995it [00:04, 1648197.13it/s] 7378486it [00:04, 1653067.55it/s] 7543834it [00:04, 1637150.71it/s] 7709118it [00:04, 1641795.84it/s] 7876668it [00:04, 1651810.75it/s] 8044953it [00:04, 1661051.81it/s] 8216552it [00:05, 1677467.43it/s] 8384325it [00:05, 1677190.94it/s] 8555326it [00:05, 1686998.05it/s] 8724040it [00:05, 1682862.79it/s] 8892339it [00:05, 1682865.32it/s] 9060634it [00:05, 1673039.72it/s] 9229473it [00:05, 1677600.43it/s] 9397305it [00:05, 1677792.06it/s] 9565211it [00:05, 1678146.78it/s] 9733172it [00:05, 1678562.64it/s] 9901034it [00:06, 1674023.16it/s] 10068443it [00:06, 1673579.30it/s] 10235805it [00:06, 1666055.62it/s] 10402962it [00:06, 1667678.93it/s] 10569737it [00:06, 1628332.79it/s] 10732770it [00:06, 1545728.86it/s] 10899209it [00:06, 1579551.86it/s] 11065168it [00:06, 1602698.55it/s] 11134598it [00:06, 1628730.83it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-804b22-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-804b22-R1_A23WJ53LT4_1/aih-tih-sc-804b22-R1_A23WJ53LT4_1.md -output aih-tih-sc-804b22-R1_A23WJ53LT4_1/aih-tih-sc-804b22-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-804b22-R1_A23WJ53LT4_1/aih-tih-sc-804b22-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-804b22-R1_A23WJ53LT4_1/aih-tih-sc-804b22-R1_A23WJ53LT4_1_full_basic_stats.tsv