Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/fd/44d478988b781361bbb43743242093/aih-tih-sc-331e97-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a4/0352010e5727c976f7d16ce88a08da/ref_annot.bed
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a8/6f78131836dab0a808636a2c67ff39/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/a8/6f78131836dab0a808636a2c67ff39/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/51/43d0eeeb57f6e794c1328833b6aa50/aih-tih-sc-331e97-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam
==> STAGING COMPLETE (5 inputs)

[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-331e97-R1_A23WJ53LT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (14403) splicing reads. 8420 splicing junctions. 8257 known splicing junctions. 163 novel splicing junctions.
sampling 10% (28807) splicing reads. 12537 splicing junctions. 12272 known splicing junctions. 265 novel splicing junctions.
sampling 15% (43211) splicing reads. 15065 splicing junctions. 14735 known splicing junctions. 330 novel splicing junctions.
sampling 20% (57615) splicing reads. 16780 splicing junctions. 16415 known splicing junctions. 365 novel splicing junctions.
sampling 25% (72019) splicing reads. 17954 splicing junctions. 17560 known splicing junctions. 394 novel splicing junctions.
sampling 30% (86423) splicing reads. 18847 splicing junctions. 18437 known splicing junctions. 410 novel splicing junctions.
sampling 35% (100827) splicing reads. 19510 splicing junctions. 19078 known splicing junctions. 432 novel splicing junctions.
sampling 40% (115231) splicing reads. 20066 splicing junctions. 19613 known splicing junctions. 453 novel splicing junctions.
sampling 45% (129635) splicing reads. 20506 splicing junctions. 20038 known splicing junctions. 468 novel splicing junctions.
sampling 50% (144039) splicing reads. 20863 splicing junctions. 20377 known splicing junctions. 486 novel splicing junctions.
sampling 55% (158442) splicing reads. 21179 splicing junctions. 20681 known splicing junctions. 498 novel splicing junctions.
sampling 60% (172846) splicing reads. 21406 splicing junctions. 20897 known splicing junctions. 509 novel splicing junctions.
sampling 65% (187250) splicing reads. 21599 splicing junctions. 21081 known splicing junctions. 518 novel splicing junctions.
sampling 70% (201654) splicing reads. 21776 splicing junctions. 21249 known splicing junctions. 527 novel splicing junctions.
sampling 75% (216058) splicing reads. 21928 splicing junctions. 21394 known splicing junctions. 534 novel splicing junctions.
sampling 80% (230462) splicing reads. 22024 splicing junctions. 21486 known splicing junctions. 538 novel splicing junctions.
sampling 85% (244866) splicing reads. 22106 splicing junctions. 21563 known splicing junctions. 543 novel splicing junctions.
sampling 90% (259270) splicing reads. 22160 splicing junctions. 21616 known splicing junctions. 544 novel splicing junctions.
sampling 95% (273674) splicing reads. 22192 splicing junctions. 21647 known splicing junctions. 545 novel splicing junctions.
sampling 100% (288078) splicing reads. 22205 splicing junctions. 21659 known splicing junctions. 546 novel splicing junctions.
null device 
          1