+ mkdir aih-tih-sc-2905df-R1_A23WJ53LT4_1 + all_qc -bam aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-2905df-R1_A23WJ53LT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai 0it [00:00, ?it/s] 140107it [00:00, 1400063.26it/s] 291661it [00:00, 1467942.66it/s] 454538it [00:00, 1541347.76it/s] 618513it [00:00, 1580046.40it/s] 786464it [00:00, 1615881.81it/s] 953405it [00:00, 1633957.04it/s] 1119925it [00:00, 1644156.33it/s] 1287639it [00:00, 1654613.68it/s] 1453101it [00:00, 1645312.87it/s] 1617709it [00:01, 1645397.25it/s] 1784619it [00:01, 1652614.81it/s] 1949889it [00:01, 1636736.54it/s] 2113600it [00:01, 1624657.14it/s] 2281677it [00:01, 1641358.16it/s] 2450648it [00:01, 1655800.86it/s] 2616270it [00:01, 1654784.62it/s] 2786367it [00:01, 1668490.35it/s] 2954186it [00:01, 1671376.38it/s] 3121503it [00:01, 1671766.29it/s] 3288693it [00:02, 1654606.22it/s] 3455604it [00:02, 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[00:08, 1674843.25it/s] 13222726it [00:08, 1676405.53it/s] 13390696it [00:08, 1677371.76it/s] 13558442it [00:08, 1674977.70it/s] 13725947it [00:08, 1667462.74it/s] 13893668it [00:08, 1670357.18it/s] 14061889it [00:08, 1669648.85it/s] 14228861it [00:08, 1669471.98it/s] 14395813it [00:08, 1668713.28it/s] 14565922it [00:08, 1678384.54it/s] 14734465it [00:09, 1680461.52it/s] 14904428it [00:09, 1686184.05it/s] 15073050it [00:09, 1668867.06it/s] 15242121it [00:09, 1675337.20it/s] 15412277it [00:09, 1683138.61it/s] 15580619it [00:09, 1674933.92it/s] 15748730it [00:09, 1676692.39it/s] 15916516it [00:09, 1677021.33it/s] 16084232it [00:09, 1672316.02it/s] 16252818it [00:09, 1676337.87it/s] 16420462it [00:10, 1671488.54it/s] 16480577it [00:10, 1635853.49it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2905df-R1_A23WJ53LT4_1.md.bai + merge_qc -folders aih-tih-sc-2905df-R1_A23WJ53LT4_1/aih-tih-sc-2905df-R1_A23WJ53LT4_1.md -output aih-tih-sc-2905df-R1_A23WJ53LT4_1/aih-tih-sc-2905df-R1_A23WJ53LT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-2905df-R1_A23WJ53LT4_1/aih-tih-sc-2905df-R1_A23WJ53LT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-2905df-R1_A23WJ53LT4_1/aih-tih-sc-2905df-R1_A23WJ53LT4_1_full_basic_stats.tsv