FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_B23WHTKLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23WHTKLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839474
Total Bases126.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC73

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGT49526258.99670507960938TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGT11092213.213274026354599TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGG749148.923921407929251TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGG486295.792794059137031TruSeq Adapter, Index 9 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTATG104821.2486390287251303TruSeq Adapter, Index 9 (97% over 34bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA40480.4822067151573486TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTGTG34590.41204373214655843TruSeq Adapter, Index 9 (97% over 34bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAG25500.3037616412181914TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCGAGT22470.2676676109087357TruSeq Adapter, Index 9 (97% over 37bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT18210.21692154849346137No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCGAGG16070.19142939507358178TruSeq Adapter, Index 9 (97% over 37bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA15760.1877366064940665No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTG14890.17737297402897528TruSeq Adapter, Index 9 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGTATG14760.17582438526982372TruSeq Adapter, Index 9 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCGGGT13420.15986200882933838TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATGTAGT12740.15176169839685325TruSeq Adapter, Index 9 (97% over 37bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGTGTG11950.14235104362970147TruSeq Adapter, Index 9 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCGGGG11890.14163631035624688TruSeq Adapter, Index 9 (97% over 37bp)
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC10690.12734164488715552No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATGTGGT9620.11459556817721574TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATTTAGT9150.10899682420182162TruSeq Adapter, Index 9 (97% over 37bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG8980.10697174659370033No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATGTAGG8900.10601876889576092TruSeq Adapter, Index 9 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATATAGT8740.10411281349988206TruSeq Adapter, Index 9 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)