Detecting adapter sequence for read1... >Nextera_LMP_Read1_External_Adapter | >Illumina Multiplexing Index Sequencing Primer GATCGGAAGAGCACACGTCTGAACTCCAGTCAC Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 839474 total bases: 126760574 Q20 bases: 110576327(87.2324%) Q30 bases: 86561368(68.2873%) Read2 before filtering: total reads: 839474 total bases: 126760574 Q20 bases: 122305259(96.4853%) Q30 bases: 103772510(81.865%) Read1 after filtering: total reads: 26975 total bases: 2853966 Q20 bases: 2705348(94.7926%) Q30 bases: 2468152(86.4815%) Read2 after filtering: total reads: 26975 total bases: 2879362 Q20 bases: 2813116(97.6993%) Q30 bases: 2642110(91.7603%) Filtering result: reads passed filter: 53950 reads failed due to low quality: 34080 reads failed due to too many N: 0 reads failed due to too short: 1590918 reads with adapter trimmed: 849756 bases trimmed due to adapters: 121426679 Duplication rate: 67.3407% Insert size peak (evaluated by paired-end reads): 36 JSON report: NTC_0001_0001_B23WHTKLT4_1.fastp.json HTML report: NTC_0001_0001_B23WHTKLT4_1.fastp.html fastp --in1 NTC_0001_0001_B23WHTKLT4_1_1.fastq.gz --in2 NTC_0001_0001_B23WHTKLT4_1_2.fastq.gz --out1 NTC_0001_0001_B23WHTKLT4_1_1.fastp.fastq.gz --out2 NTC_0001_0001_B23WHTKLT4_1_2.fastp.fastq.gz --json NTC_0001_0001_B23WHTKLT4_1.fastp.json --html NTC_0001_0001_B23WHTKLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 4 seconds