Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 52007000 total bases: 7853057000 Q20 bases: 7739077419(98.5486%) Q30 bases: 7516447113(95.7136%) Read2 before filtering: total reads: 52007000 total bases: 7853057000 Q20 bases: 7699883349(98.0495%) Q30 bases: 7391860632(94.1272%) Read1 after filtering: total reads: 50612085 total bases: 6680646061 Q20 bases: 6656266028(99.6351%) Q30 bases: 6536802988(97.8469%) Read2 after filtering: total reads: 50612085 total bases: 6684916402 Q20 bases: 6641779559(99.3547%) Q30 bases: 6482505680(96.9721%) Filtering result: reads passed filter: 101224170 reads failed due to low quality: 1647966 reads failed due to too many N: 3932 reads failed due to too short: 1137932 reads with adapter trimmed: 54360901 bases trimmed due to adapters: 1958248624 Duplication rate: 69.2225% Insert size peak (evaluated by paired-end reads): 120 JSON report: tih_rna_sample_00461_B23WHTKLT4_1.fastp.json HTML report: tih_rna_sample_00461_B23WHTKLT4_1.fastp.html fastp --in1 tih_rna_sample_00461_B23WHTKLT4_1_1.fastq.gz --in2 tih_rna_sample_00461_B23WHTKLT4_1_2.fastq.gz --out1 tih_rna_sample_00461_B23WHTKLT4_1_1.fastp.fastq.gz --out2 tih_rna_sample_00461_B23WHTKLT4_1_2.fastp.fastq.gz --json tih_rna_sample_00461_B23WHTKLT4_1.fastp.json --html tih_rna_sample_00461_B23WHTKLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 99 seconds