FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_B23WHTKLT4_1_trimmed_2.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23WHTKLT4_1_trimmed_2.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26975
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC69

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG501118.576459684893422No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA23888.852641334569046No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC20697.670064874884153No Hit
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC7772.8804448563484706No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG5572.0648748841519926No Hit
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC1740.6450417052826691No Hit
GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC880.3262279888785913No Hit
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC560.2075996292863763No Hit
TCTGGCTGCCGACAGTCAGGTTCTGCTGCAGAAGCACGTTCTCTATGCAG490.18164967562557924No Hit
CATTGTGGTAATTGGCATGCTGGCCACGGGCCTCAGTCCCACCACCCGTA480.17794253938832252No Hit
GCGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA450.16682113067655235No Hit
CTGCCATTGCAGGTTTGGATATCCTTCTGCCAGCAAAACCCAGCATGAAC410.15199258572752547No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGC410.15199258572752547No Hit
ATGGGAATCCATTGGCGCTTCGGACAAGTCCTTCCCCTGCTCCGCCTTGA380.14087117701575533No Hit
CCCCGTCCTCCCCCCTCCCCGGGG370.1371640407784986No Hit
GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC360.1334569045412419No Hit
GGCATGTACTTGCCCCGCGCTGTGCTCGTGGATCTGGAGCTGGGCGCCAT360.1334569045412419No Hit
GGCAGCCGCAGGGGCTTCAGCACCACCTCGGCCATCACCCCGGCAGCTGG350.12974976830398519No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG350.12974976830398519No Hit
AAAGTAATAGCTACTATTTTTTCTCTTTCAGTTGCAGGAGAAATAACAAG330.12233549582947173No Hit
CCCCCCTGTGCCCAGGGAAATGTTTCAGGCTTTCCCCGGAGACTACGACT330.12233549582947173No Hit
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC320.11862835959221502No Hit
TCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC310.1149212233549583No Hit
GACTGGAGTTCAGACGTGTGCTCTTCCGATC310.1149212233549583Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CTTCGCGCCCTCTCTCTCTCTCTCTCCC310.1149212233549583No Hit
CTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT290.10750695088044486No Hit
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG290.10750695088044486No Hit
TTTCAAAAGTGGCTTATGCTGTAGCAGGGGGCCCTGAACGTCTGTTTTCT280.10379981464318815No Hit
GGCTTGACATGTTCCCTGTAAAACTCCAAGGGTGTTATGGGGCCAATTTT280.10379981464318815No Hit
GACATGAAGGTGCAGAGGGAGACAGATGAGGTGAAATAGAAGGTCATGCT280.10379981464318815No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCC270.1000926784059314No Hit
TCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC270.1000926784059314No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGG270.1000926784059314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)