FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_B23WHTKLT4_1_trimmed_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_B23WHTKLT4_1_trimmed_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26975
Total Bases2.8 Mbp
Sequences flagged as poor quality0
Sequence length15-151
%GC62

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT18156.728452270620945No Hit
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA15305.67191844300278No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC10653.948100092678406No Hit
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG8823.2696941612604267No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGC7652.83595922150139No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA2891.071362372567192TruSeq Adapter, Index 1 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACAATCTGCAGTATCTAGTA2440.9045412418906394Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAG2340.8674698795180723TruSeq Adapter, Index 1 (96% over 30bp)
GCTGCCGGGGGGGCTGTAACACTCGGCAGCA1720.6376274328081557No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACAATCTGCAGTATCTAGTA1450.5375347544022243Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAACTCCGTCACAATCTGCAGTATCTAGTA1320.4893419833178869Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTG1290.4782205746061168TruSeq Adapter, Index 1 (96% over 29bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACAATCTGCAGTATCTAG1010.3744207599629287Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACACGTCTGAACTCCATCACAATCTGCAGTATCTAGTA900.3336422613531047Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACAATCTGCAGTATCTAGTG860.31881371640407785Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCACACGTCTGAACCCAGTCACAATCTGCAGTATCTAGTA860.31881371640407785Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGCAGCAGC830.3076923076923077No Hit
GATCGGAAGAGCACCGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA800.2965708989805375TruSeq Adapter, Index 9 (95% over 22bp)
GATCGGAAGAGCACACGTCTAACTCCAGTCACAATCTGCAGTATCTAGTA770.28544949026876737Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGG620.2298424467099166TruSeq Adapter, Index 1 (96% over 30bp)
GATCGGAAGAGCACACCGTCTGAACTCCAGTCACAATCTGCAGTATCTAG620.2298424467099166No Hit
GATCGGAAGAGCACCACGTCTGAACTCCAGTCACAATCTGCAGTATCTAG590.21872103799814643TruSeq Adapter, Index 1 (95% over 22bp)
GATCGGAAGAGCACACGTCTGAAACTCCAGTCACAATCTGCAGTATCTAG570.21130676552363298Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCGGAAGAGCACACGTTCTGAACTCCAGTCACAATCTGCAGTATCTAG570.21130676552363298No Hit
GATCGGAAGAGCACACGTCCTGAACTCCAGTCACAATCTGCAGTATCTAG560.2075996292863763No Hit
GGGGGAGAGAGAGAGAGAGAGGGCGCGAAGA550.20389249304911955No Hit
GATCGGAAGAGCACACGCTGAACTCCAGTCACAATCTGCAGTATCTAGTA530.19647822057460612No Hit
GATCGGAAGAGCACACGTCTGACTCCAGTCACAATCTGCAGTATCTAGTG530.19647822057460612Illumina Multiplexing PCR Primer 2.01 (95% over 22bp)
GATCGGAAGAGCACACGTCGAACTCCAGTCACAATCTGCAGTATCTAGTA530.19647822057460612No Hit
GATCGGAAGAGCACACGTCTGAACTCCGTCACAATCTGCAGTATCTAGTG520.19277108433734938Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCAGGCTCTGCAGAATGACACCGTCTTCTTGAACATGCGGGGCTTGTATG500.18535681186283595No Hit
GATCGGAAGAGCACACGTCTTGAACTCCAGTCACAATCTGCAGTATCTAG500.18535681186283595No Hit
GATCGGAAGAGCCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAG490.18164967562557924TruSeq Adapter, Index 1 (95% over 24bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGTG480.17794253938832252TruSeq Adapter, Index 1 (96% over 29bp)
CGGGAACGTATTCACCGTGGCGTGCTGATCCACGATTACTAGCGATTCCA470.1742354031510658No Hit
GACATGAAGGTGCAGAGGGAGACAGATGAGGTGAAATAGAAGGTCATGCT460.1705282669138091No Hit
GATCGGAAGAGCACACGTCTGAACTCAGTCACAATCTGCAGTATCTGGTA440.16311399443929564Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
GATCGGAAGAGCCACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA430.15940685820203893TruSeq Adapter, Index 9 (95% over 24bp)
GGAAGTTAATGAGAGACAGTCCCATCCAGCTACGCAGCAGCAGCTGGGCA420.1556997219647822No Hit
GATCGGAAGAGCACACGTCTGAACTTCCAGTCACAATCTGCAGTATCTAG420.1556997219647822Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
GATCGGAAGAGCACAACGTCTGAACTCCAGTCACAATCTGCAGTATCTAG400.14828544949026876TruSeq Adapter, Index 9 (95% over 23bp)
AAAGTAATAGCTACTATTTTTTCTCTTTCAGTTGCAGGAGAAATAACAAG390.14457831325301204No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGGG380.14087117701575533TruSeq Adapter, Index 1 (96% over 29bp)
GATCGGAAGAGCAACACGTCTGAACTCCAGTCACAATCTGCAGTATCTAG370.1371640407784986TruSeq Adapter, Index 9 (96% over 25bp)
GCTGCCACACCCATTGATGGAGACCCGCTTGGCACCCCCCAGGTTGTAGA370.1371640407784986No Hit
GCGCCCTCTCTCTCTCTCTCTCCCCCGCA370.1371640407784986No Hit
GATCGGAAGAGCACACGTCTGGAACTCCAGTCACAATCTGCAGTATCTAG370.1371640407784986Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
GATCGGAAGAGCAACGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA370.1371640407784986TruSeq Adapter, Index 1 (95% over 21bp)
TGAAGTTGTCTGGCCTGAAGTTCTGCCCCAAGGGCCCCGAGCGCACAGAG360.1334569045412419No Hit
GATCGGAAGAGCACACGGTCTGAACTCCAGTCACAATCTGCAGTATCTAG360.1334569045412419TruSeq Adapter, Index 9 (95% over 21bp)
GGCCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGCAC360.1334569045412419No Hit
GCCCTCACCTCTCCGTCTCTCCGTGCCTTCCCCTCCAGGCCCCTTTGGCC350.12974976830398519No Hit
CCCCGGGGAGGGGGGAGGACGGGGA350.12974976830398519No Hit
TCTGATGCGCCTTTTTGTGGGGGTTGGGGTTTATTCTTTTCACCCTTGAC350.12974976830398519No Hit
GATCGGAAGAGCACACGTCTGAACCTCCAGTCACAATCTGCAGTATCTAG350.12974976830398519Illumina Multiplexing PCR Primer 2.01 (95% over 24bp)
GATCGGAAGAGCACAGTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA330.12233549582947173No Hit
GATCGGAAGAGCACACGTCTGAACTCCATCACAATCTGCAGTATCTAGTG320.11862835959221502Illumina Multiplexing PCR Primer 2.01 (96% over 28bp)
ATGGGAATCCATTGGCGCTTCGGACAAGTCCTTCCCCTGCTCCGCCTTGA320.11862835959221502No Hit
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGTA320.11862835959221502TruSeq Adapter, Index 1 (96% over 29bp)
GATCGGAGAGCACACGTCTGAACTCCAGTCACAATCTGCAGTATCTGGGG320.11862835959221502TruSeq Adapter, Index 1 (96% over 29bp)
GGGAGAGAGAGAGAGAGAGGGCGCGAAGA310.1149212233549583No Hit
GATCGGAAGAGCACACTCTGAACTCCAGTCACAATCTGCAGTATCTAGTA310.1149212233549583No Hit
GCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC310.1149212233549583No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTC310.1149212233549583Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
GATCGGAAGAGCACACGTCTGAACTCCCAGTCACAATCTGCAGTATCTGG300.11121408711770157Illumina Multiplexing PCR Primer 2.01 (96% over 27bp)
CCGAGTGTTACAGCCCCCCCGGCAGCAGCA280.10379981464318815No Hit
CGCGCCCTCTCTCTCTCTCTCTCCCCCGCA280.10379981464318815No Hit
GCCTTTCCTCCTCCTCTCTTCCAGATATTCCGAGTGGTGTGCATCTGTTT280.10379981464318815No Hit
GATCGGAAGAGCACACGTCTGAATCCAGTCACAATCTGCAGTATCTAGTA280.10379981464318815Illumina Multiplexing PCR Primer 2.01 (95% over 23bp)
TGCCAATGTTGTAGTTCCACATCTTCATTGTGCCATCCCGCAAACCTGTG280.10379981464318815No Hit
CATTGTGGTAATTGGCATGCTGGCCACGGGCCTCAGTCCCACCACCCGTA270.1000926784059314No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)