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        If you use plots from MultiQC in a publication or presentation, please cite:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

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        About MultiQC

        This report was generated using MultiQC, version 1.21

        You can see a YouTube video describing how to use MultiQC reports here: https://youtu.be/qPbIlO_KWN0

        For more information about MultiQC, including other videos and extensive documentation, please visit http://multiqc.info

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        MultiQC is published in Bioinformatics:

        MultiQC: Summarize analysis results for multiple tools and samples in a single report
        Philip Ewels, Måns Magnusson, Sverker Lundin and Max Käller
        Bioinformatics (2016)
        doi: 10.1093/bioinformatics/btw354
        PMID: 27312411

        A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

        This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the documentation.

        Report generated on 2026-06-08, 13:28 UTC based on data in: /tmp/nxf.Rhbf7SWtEM


        General Statistics

        Showing 480/480 rows and 55/61 columns.
        Sample NameFragment Length MeanFragment Length Medianpct Mito est. countspct Ribo est. countspct Housekeeping est. countspct Male est. countspct Mito TPMpct Ribo TPMpct Housekeeping TPMpct Male TPMChrY Signature ScoreTotal ReadsExpected DistinctRNA contaminatedRNA contamination %ReadsFrac UnmappedFrac DupesUnique ReadsFrac Reads On TargetFrac Pairs On TargetFrac Reads Off TargetFrac Pairs Off TargetReads On TargetPairs On TargetReads Off TargetPairs Off TargetMean DepthQ50 DepthFrac Bases Gt30XFrac Bases Gt100XN Q30GC BiasUnique ReadsFrac AntisenseFrag Length% AlignedM AlignedDuplication% Proper Pairs% AlignedM Aligned% DuplicationM Reads After FilteringGC content% PF% Adapter% Dups% GCMedian Read LengthM Seqs% Dups% GCMedian Read LengthM Seqs
        659_6i-T1-TRNA-1_B23WHTKLT4_1
        130.2bp
        123.0bp
        0.01%
        1.35%
        0.40%
        0.00%
        0.01%
        4.11%
        0.63%
        0.00%
        6.40
        9999000000
        54417571
        No
        0.00%
        385044116
        0.01%
        0.87%
        51885988
        0.82%
        0.87%
        0.13%
        0.13%
        42648633
        21454637
        6969430
        3149941
        66.08
        30.00
        0.50%
        0.19%
        0.98
        0.000
        372333557
        0.8627
        87.0%
        88.7%
        95.5%
        183.9M
        62.6%
        385.0M
        54.7%
        98.5%
        70.5%
        659_6i-T1-TRNA-1_B23WHTKLT4_1_1
        130.2bp
        97.6%
        187.9M
        88.9%
        53%
        151bp
        195.4M
        659_6i-T1-TRNA-1_B23WHTKLT4_1_2
        87.6%
        54%
        151bp
        195.4M
        659_6i-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.0%
        54%
        127bp
        192.5M
        89.0%
        54%
        127bp
        192.5M
        659_6i-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.7%
        54%
        127bp
        192.5M
        88.7%
        54%
        127bp
        192.5M
        659_Dl-T1-TRNA-1_B23WHTKLT4_1
        135.1bp
        126.0bp
        0.01%
        1.43%
        0.42%
        0.00%
        0.01%
        4.63%
        0.72%
        0.00%
        0.01
        9999000000
        40975613
        No
        0.00%
        300715434
        0.01%
        0.89%
        32266285
        0.77%
        0.84%
        0.16%
        0.16%
        24947277
        12561194
        5159792
        2325539
        40.34
        16.00
        0.36%
        0.10%
        0.97
        0.000
        286646997
        0.8595
        89.9%
        85.1%
        94.4%
        141.9M
        64.1%
        300.7M
        56.1%
        98.2%
        65.5%
        659_Dl-T1-TRNA-1_B23WHTKLT4_1_1
        135.1bp
        97.1%
        146.0M
        90.0%
        55%
        151bp
        153.1M
        659_Dl-T1-TRNA-1_B23WHTKLT4_1_2
        88.8%
        55%
        151bp
        153.1M
        659_Dl-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        90.1%
        55%
        132bp
        150.4M
        90.1%
        55%
        132bp
        150.4M
        659_Dl-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.9%
        56%
        132bp
        150.4M
        89.9%
        56%
        132bp
        150.4M
        659_Lx-T1-TRNA-1_B23WHTKLT4_1
        129.3bp
        119.0bp
        0.01%
        1.08%
        0.36%
        0.00%
        0.01%
        3.48%
        0.60%
        0.00%
        0.05
        9999000000
        94257245
        No
        0.00%
        496371104
        0.00%
        0.79%
        102189153
        0.90%
        0.93%
        0.08%
        0.07%
        91840320
        46192853
        8224644
        3462018
        147.84
        60.00
        0.61%
        0.39%
        0.98
        0.000
        483184782
        0.9268
        79.8%
        90.7%
        96.2%
        238.7M
        52.9%
        496.4M
        54.3%
        98.3%
        70.6%
        659_Lx-T1-TRNA-1_B23WHTKLT4_1_1
        129.3bp
        98.4%
        244.2M
        87.8%
        53%
        151bp
        252.5M
        659_Lx-T1-TRNA-1_B23WHTKLT4_1_2
        86.6%
        54%
        151bp
        252.5M
        659_Lx-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        87.8%
        54%
        122bp
        248.2M
        87.8%
        54%
        122bp
        248.2M
        659_Lx-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.0%
        54%
        122bp
        248.2M
        88.0%
        54%
        122bp
        248.2M
        659_Mt-T1-TRNA-1_B23WHTKLT4_1
        140.2bp
        129.0bp
        0.00%
        0.84%
        0.30%
        0.00%
        0.01%
        2.74%
        0.50%
        0.00%
        0.21
        9999000000
        46895114
        No
        0.00%
        329558042
        0.01%
        0.86%
        44757569
        0.83%
        0.88%
        0.13%
        0.12%
        37128638
        18701127
        5773680
        2515349
        62.59
        24.00
        0.46%
        0.16%
        0.97
        0.000
        315707016
        0.8989
        86.9%
        86.5%
        94.9%
        156.3M
        62.6%
        329.6M
        55.1%
        98.1%
        61.4%
        659_Mt-T1-TRNA-1_B23WHTKLT4_1_1
        140.2bp
        97.5%
        160.6M
        89.4%
        54%
        151bp
        167.9M
        659_Mt-T1-TRNA-1_B23WHTKLT4_1_2
        87.9%
        55%
        151bp
        167.9M
        659_Mt-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.4%
        54%
        132bp
        164.8M
        89.4%
        54%
        132bp
        164.8M
        659_Mt-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.3%
        55%
        137bp
        164.8M
        89.3%
        55%
        137bp
        164.8M
        659_bCu-T1-TRNA-1_B23WHTKLT4_1
        121.1bp
        112.0bp
        0.00%
        0.68%
        0.24%
        0.00%
        0.00%
        2.26%
        0.40%
        0.00%
        0.37
        9999000000
        16041081
        No
        0.00%
        134932238
        0.01%
        0.91%
        12624538
        0.72%
        0.80%
        0.20%
        0.20%
        9072505
        4535875
        2522077
        1160273
        13.35
        3.00
        0.10%
        0.02%
        0.97
        0.000
        129450316
        0.9343
        91.3%
        86.6%
        94.9%
        64.1M
        54.7%
        134.9M
        59.2%
        96.8%
        74.6%
        659_bCu-T1-TRNA-1_B23WHTKLT4_1_1
        121.1bp
        97.2%
        65.6M
        90.9%
        57%
        151bp
        69.7M
        659_bCu-T1-TRNA-1_B23WHTKLT4_1_2
        88.7%
        58%
        151bp
        69.7M
        659_bCu-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        90.9%
        59%
        117bp
        67.5M
        90.9%
        59%
        117bp
        67.5M
        659_bCu-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        90.4%
        59%
        117bp
        67.5M
        90.4%
        59%
        117bp
        67.5M
        659_bGW-T1-TRNA-1_B23WHTKLT4_1
        143.4bp
        131.0bp
        0.01%
        1.28%
        0.66%
        0.00%
        0.02%
        4.10%
        0.98%
        0.00%
        0.01
        9999000000
        116615565
        No
        0.00%
        591135768
        0.00%
        0.76%
        138918583
        0.92%
        0.95%
        0.06%
        0.05%
        127425160
        64439192
        8653144
        3123651
        232.20
        87.00
        0.65%
        0.47%
        0.97
        0.000
        571317819
        0.9224
        76.9%
        88.9%
        95.6%
        282.7M
        55.5%
        591.1M
        56.9%
        98.5%
        58.5%
        659_bGW-T1-TRNA-1_B23WHTKLT4_1_1
        143.4bp
        98.4%
        291.0M
        91.2%
        55%
        151bp
        470.6M
        659_bGW-T1-TRNA-1_B23WHTKLT4_1_2
        89.9%
        56%
        151bp
        470.6M
        659_bGW-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.5%
        56%
        137bp
        295.6M
        88.5%
        56%
        137bp
        295.6M
        659_bGW-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.5%
        57%
        137bp
        295.6M
        88.5%
        57%
        137bp
        295.6M
        659_bGc-T1-TRNA-1_B23WHTKLT4_1
        135.9bp
        124.0bp
        0.00%
        0.97%
        0.39%
        0.00%
        0.01%
        2.94%
        0.59%
        0.00%
        0.05
        9999000000
        66524871
        No
        0.00%
        381979332
        0.01%
        0.82%
        68115683
        0.88%
        0.92%
        0.09%
        0.08%
        59743611
        30129541
        6298607
        2574850
        100.82
        41.00
        0.56%
        0.28%
        0.97
        0.000
        370761931
        0.9132
        82.6%
        90.4%
        96.1%
        183.5M
        55.0%
        382.0M
        56.3%
        98.2%
        64.6%
        659_bGc-T1-TRNA-1_B23WHTKLT4_1_1
        135.9bp
        98.1%
        187.3M
        88.2%
        55%
        151bp
        194.5M
        659_bGc-T1-TRNA-1_B23WHTKLT4_1_2
        86.5%
        56%
        151bp
        194.5M
        659_bGc-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.3%
        56%
        127bp
        191.0M
        88.3%
        56%
        127bp
        191.0M
        659_bGc-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.9%
        56%
        132bp
        191.0M
        87.9%
        56%
        132bp
        191.0M
        659_bNS-T1-TRNA-1_B23WHTKLT4_1
        152.7bp
        138.0bp
        0.00%
        1.46%
        0.56%
        0.00%
        0.01%
        4.36%
        0.85%
        0.00%
        5.86
        9999000000
        103263588
        No
        0.00%
        591123330
        0.01%
        0.82%
        107953649
        0.89%
        0.94%
        0.08%
        0.06%
        96327878
        48883028
        8611157
        3115326
        182.08
        71.00
        0.63%
        0.43%
        0.97
        0.000
        573849300
        0.9153
        82.2%
        90.5%
        96.1%
        284.1M
        59.9%
        591.1M
        56.3%
        98.5%
        52.1%
        659_bNS-T1-TRNA-1_B23WHTKLT4_1_1
        152.7bp
        98.3%
        290.4M
        90.5%
        55%
        151bp
        341.4M
        659_bNS-T1-TRNA-1_B23WHTKLT4_1_2
        89.4%
        56%
        151bp
        341.4M
        659_bNS-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.9%
        55%
        147bp
        295.6M
        89.9%
        55%
        147bp
        295.6M
        659_bNS-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.9%
        56%
        147bp
        295.6M
        89.9%
        56%
        147bp
        295.6M
        659_bQZ-T1-TRNA-1_B23WHTKLT4_1
        144.4bp
        133.0bp
        0.00%
        1.47%
        0.57%
        0.00%
        0.01%
        4.41%
        0.88%
        0.00%
        0.01
        9999000000
        87380240
        No
        0.00%
        547763984
        0.01%
        0.83%
        94459755
        0.88%
        0.93%
        0.08%
        0.07%
        82836670
        41896859
        7963890
        3088648
        150.44
        58.00
        0.61%
        0.38%
        0.97
        0.000
        527373127
        0.9119
        83.3%
        88.5%
        95.3%
        261.1M
        58.8%
        547.8M
        57.3%
        98.4%
        57.7%
        659_bQZ-T1-TRNA-1_B23WHTKLT4_1_1
        144.4bp
        97.9%
        268.0M
        89.3%
        56%
        151bp
        278.3M
        659_bQZ-T1-TRNA-1_B23WHTKLT4_1_2
        88.2%
        57%
        151bp
        278.3M
        659_bQZ-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.4%
        57%
        137bp
        273.9M
        89.4%
        57%
        137bp
        273.9M
        659_bQZ-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.5%
        57%
        137bp
        273.9M
        89.5%
        57%
        137bp
        273.9M
        659_bTo-T1-TRNA-1_B23WHTKLT4_1
        160.2bp
        145.0bp
        0.00%
        0.76%
        0.68%
        0.00%
        0.01%
        2.45%
        1.02%
        0.00%
        0.00
        9999000000
        153203027
        No
        0.00%
        592110160
        0.00%
        0.69%
        182833146
        0.93%
        0.97%
        0.05%
        0.03%
        170927737
        86639890
        9483671
        2943700
        347.14
        116.00
        0.66%
        0.52%
        0.97
        0.000
        578719959
        0.9268
        69.4%
        92.5%
        96.8%
        286.6M
        50.8%
        592.1M
        56.0%
        98.7%
        47.9%
        659_bTo-T1-TRNA-1_B23WHTKLT4_1_1
        160.2bp
        98.5%
        291.8M
        90.2%
        55%
        151bp
        434.3M
        659_bTo-T1-TRNA-1_B23WHTKLT4_1_2
        89.2%
        56%
        151bp
        434.3M
        659_bTo-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.0%
        55%
        151bp
        296.1M
        88.0%
        55%
        151bp
        296.1M
        659_bTo-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.2%
        56%
        151bp
        296.1M
        88.2%
        56%
        151bp
        296.1M
        659_bUW-T1-TRNA-1_B23WHTKLT4_1
        159.0bp
        142.0bp
        0.01%
        1.64%
        0.66%
        0.00%
        0.01%
        4.99%
        1.00%
        0.00%
        0.00
        9999000000
        183866615
        No
        0.00%
        592010030
        0.00%
        0.63%
        219072105
        0.94%
        0.97%
        0.05%
        0.03%
        205739160
        104332465
        10613248
        3232811
        421.26
        149.00
        0.67%
        0.55%
        0.97
        0.000
        562585333
        0.9375
        63.3%
        86.0%
        94.2%
        278.9M
        45.3%
        592.0M
        54.3%
        98.7%
        49.0%
        659_bUW-T1-TRNA-1_B23WHTKLT4_1_1
        159.0bp
        98.6%
        291.8M
        91.3%
        53%
        151bp
        617.4M
        659_bUW-T1-TRNA-1_B23WHTKLT4_1_2
        90.5%
        54%
        151bp
        617.4M
        659_bUW-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        87.1%
        54%
        151bp
        296.0M
        87.1%
        54%
        151bp
        296.0M
        659_bUW-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.6%
        54%
        151bp
        296.0M
        87.6%
        54%
        151bp
        296.0M
        659_bhK-T1-TRNA-1_B23WHTKLT4_1
        156.6bp
        142.0bp
        0.00%
        0.68%
        0.37%
        0.00%
        0.01%
        2.53%
        0.65%
        0.00%
        5.90
        9999000000
        101009619
        No
        0.00%
        591713216
        0.01%
        0.78%
        128752902
        0.91%
        0.95%
        0.07%
        0.05%
        116848877
        59289011
        8913463
        3126263
        229.43
        88.00
        0.65%
        0.48%
        0.97
        0.000
        577370866
        0.9131
        78.6%
        92.4%
        96.7%
        286.2M
        57.6%
        591.7M
        56.4%
        98.6%
        49.7%
        659_bhK-T1-TRNA-1_B23WHTKLT4_1_1
        156.6bp
        98.3%
        290.9M
        91.8%
        55%
        151bp
        481.3M
        659_bhK-T1-TRNA-1_B23WHTKLT4_1_2
        90.6%
        56%
        151bp
        481.3M
        659_bhK-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.3%
        56%
        151bp
        295.9M
        89.3%
        56%
        151bp
        295.9M
        659_bhK-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.2%
        56%
        151bp
        295.9M
        89.2%
        56%
        151bp
        295.9M
        659_buD-T1-TRNA-1_B23WHTKLT4_1
        139.3bp
        126.0bp
        0.01%
        1.19%
        0.61%
        0.00%
        0.02%
        3.67%
        0.92%
        0.00%
        0.01
        9999000000
        115250997
        No
        0.00%
        436345888
        0.01%
        0.67%
        143877527
        0.92%
        0.95%
        0.06%
        0.05%
        132409293
        66883146
        8942056
        3318502
        238.15
        111.00
        0.69%
        0.52%
        0.97
        0.000
        422950455
        0.9252
        67.4%
        90.1%
        95.9%
        209.2M
        44.1%
        436.3M
        54.3%
        98.3%
        61.4%
        659_buD-T1-TRNA-1_B23WHTKLT4_1_1
        139.3bp
        98.2%
        214.3M
        85.0%
        53%
        151bp
        221.9M
        659_buD-T1-TRNA-1_B23WHTKLT4_1_2
        84.0%
        54%
        151bp
        221.9M
        659_buD-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        85.0%
        54%
        132bp
        218.2M
        85.0%
        54%
        132bp
        218.2M
        659_buD-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        85.2%
        54%
        132bp
        218.2M
        85.2%
        54%
        132bp
        218.2M
        659_bua-T1-TRNA-1_B23WHTKLT4_1
        147.3bp
        135.0bp
        0.00%
        0.71%
        0.54%
        0.00%
        0.01%
        2.33%
        0.81%
        0.00%
        0.02
        9999000000
        94528461
        No
        0.00%
        530013608
        0.00%
        0.81%
        102407460
        0.89%
        0.93%
        0.08%
        0.07%
        91379033
        46232704
        8569814
        3336675
        168.83
        57.00
        0.58%
        0.39%
        0.97
        0.000
        516686771
        0.9005
        81.1%
        91.7%
        96.5%
        255.8M
        57.9%
        530.0M
        55.1%
        98.7%
        55.6%
        659_bua-T1-TRNA-1_B23WHTKLT4_1_1
        147.3bp
        98.1%
        260.1M
        88.6%
        54%
        151bp
        268.6M
        659_bua-T1-TRNA-1_B23WHTKLT4_1_2
        87.4%
        55%
        151bp
        268.6M
        659_bua-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.7%
        54%
        142bp
        265.0M
        88.7%
        54%
        142bp
        265.0M
        659_bua-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.5%
        55%
        142bp
        265.0M
        88.5%
        55%
        142bp
        265.0M
        659_c95-T1-TRNA-1_B23WHTKLT4_1
        153.0bp
        138.0bp
        0.01%
        1.07%
        0.46%
        0.00%
        0.01%
        3.36%
        0.69%
        0.00%
        0.17
        9990000000
        175305185
        Yes
        0.00%
        591546962
        0.01%
        0.64%
        210745899
        0.93%
        0.96%
        0.06%
        0.04%
        195104396
        98931905
        12607303
        4326747
        383.03
        175.00
        0.71%
        0.59%
        0.97
        0.000
        572351141
        0.8757
        64.7%
        89.3%
        95.9%
        283.5M
        45.0%
        591.5M
        54.7%
        98.6%
        52.0%
        659_c95-T1-TRNA-1_B23WHTKLT4_1_1
        153.0bp
        97.9%
        289.4M
        86.9%
        53%
        151bp
        341.7M
        659_c95-T1-TRNA-1_B23WHTKLT4_1_2
        86.2%
        54%
        151bp
        341.7M
        659_c95-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        86.0%
        54%
        147bp
        295.8M
        86.0%
        54%
        147bp
        295.8M
        659_c95-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        86.4%
        55%
        147bp
        295.8M
        86.4%
        55%
        147bp
        295.8M
        659_cAd-T1-TRNA-1_B23WHTKLT4_1
        132.3bp
        122.0bp
        0.01%
        1.18%
        0.69%
        0.00%
        0.01%
        3.63%
        1.05%
        0.00%
        0.01
        9999000000
        83743981
        No
        0.00%
        589833654
        0.01%
        0.84%
        93539989
        0.86%
        0.91%
        0.10%
        0.09%
        80899663
        40750889
        9063935
        3819907
        132.18
        48.00
        0.58%
        0.35%
        0.98
        0.000
        570480740
        0.9005
        84.7%
        89.3%
        95.7%
        282.1M
        56.1%
        589.8M
        55.8%
        98.3%
        66.5%
        659_cAd-T1-TRNA-1_B23WHTKLT4_1_1
        132.3bp
        98.1%
        289.2M
        90.3%
        55%
        151bp
        321.2M
        659_cAd-T1-TRNA-1_B23WHTKLT4_1_2
        89.3%
        55%
        151bp
        321.2M
        659_cAd-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.9%
        55%
        127bp
        294.9M
        89.9%
        55%
        127bp
        294.9M
        659_cAd-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        90.2%
        56%
        127bp
        294.9M
        90.2%
        56%
        127bp
        294.9M
        659_cIX-T1-TRNA-1_B23WHTKLT4_1
        146.5bp
        134.0bp
        0.00%
        0.93%
        0.52%
        0.00%
        0.01%
        3.12%
        0.81%
        0.00%
        0.02
        9999000000
        108532177
        No
        0.00%
        591645764
        0.00%
        0.79%
        127198019
        0.90%
        0.94%
        0.08%
        0.06%
        114856230
        58106363
        9733068
        3720167
        216.06
        78.00
        0.63%
        0.45%
        0.98
        0.000
        577178263
        0.9152
        78.8%
        91.7%
        96.7%
        285.9M
        57.3%
        591.6M
        55.9%
        98.6%
        55.1%
        659_cIX-T1-TRNA-1_B23WHTKLT4_1_1
        146.5bp
        98.2%
        290.5M
        88.9%
        54%
        151bp
        308.3M
        659_cIX-T1-TRNA-1_B23WHTKLT4_1_2
        87.9%
        55%
        151bp
        308.3M
        659_cIX-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.8%
        55%
        142bp
        295.8M
        88.8%
        55%
        142bp
        295.8M
        659_cIX-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.8%
        56%
        142bp
        295.8M
        88.8%
        56%
        142bp
        295.8M
        659_cRB-T1-TRNA-1_B23WHTKLT4_1
        120.8bp
        114.0bp
        0.01%
        1.07%
        0.62%
        0.00%
        0.02%
        3.50%
        0.97%
        0.00%
        6.13
        9999000000
        72620135
        No
        0.00%
        487531190
        0.01%
        0.84%
        76067949
        0.86%
        0.91%
        0.10%
        0.09%
        65778109
        33057420
        7619103
        3357796
        101.50
        42.00
        0.56%
        0.29%
        0.98
        0.000
        473295749
        0.8879
        84.9%
        89.7%
        95.9%
        233.7M
        52.3%
        487.5M
        55.8%
        98.5%
        75.6%
        659_cRB-T1-TRNA-1_B23WHTKLT4_1_1
        120.8bp
        98.2%
        239.5M
        88.8%
        54%
        151bp
        247.4M
        659_cRB-T1-TRNA-1_B23WHTKLT4_1_2
        87.7%
        55%
        151bp
        247.4M
        659_cRB-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.9%
        55%
        117bp
        243.8M
        88.9%
        55%
        117bp
        243.8M
        659_cRB-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.8%
        55%
        117bp
        243.8M
        88.8%
        55%
        117bp
        243.8M
        659_cRa-T1-TRNA-1_B23WHTKLT4_1
        151.0bp
        137.0bp
        0.00%
        0.89%
        0.49%
        0.00%
        0.01%
        3.18%
        0.81%
        0.00%
        0.02
        9999000000
        119017164
        No
        0.00%
        591351872
        0.01%
        0.76%
        142903043
        0.91%
        0.95%
        0.06%
        0.05%
        130636624
        66122578
        9225341
        3309832
        256.21
        110.00
        0.68%
        0.52%
        0.97
        0.000
        576307173
        0.9010
        76.2%
        91.7%
        96.5%
        285.3M
        55.4%
        591.4M
        56.4%
        98.6%
        52.2%
        659_cRa-T1-TRNA-1_B23WHTKLT4_1_1
        151.0bp
        98.4%
        290.9M
        91.3%
        55%
        151bp
        444.4M
        659_cRa-T1-TRNA-1_B23WHTKLT4_1_2
        90.0%
        56%
        151bp
        444.4M
        659_cRa-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.1%
        56%
        147bp
        295.7M
        89.1%
        56%
        147bp
        295.7M
        659_cRa-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.0%
        56%
        147bp
        295.7M
        89.0%
        56%
        147bp
        295.7M
        659_cTi-T1-TRNA-1_B23WHTKLT4_1
        143.1bp
        130.0bp
        0.01%
        0.94%
        0.52%
        0.00%
        0.01%
        2.94%
        0.80%
        0.00%
        0.01
        9999000000
        114506445
        No
        0.00%
        589872656
        0.01%
        0.76%
        139750592
        0.91%
        0.95%
        0.07%
        0.05%
        127136529
        64236427
        9221181
        3420279
        230.74
        107.00
        0.70%
        0.52%
        0.97
        0.000
        572405214
        0.9255
        76.7%
        90.7%
        96.0%
        283.1M
        53.3%
        589.9M
        54.9%
        98.3%
        58.5%
        659_cTi-T1-TRNA-1_B23WHTKLT4_1_1
        143.1bp
        98.2%
        289.5M
        89.9%
        54%
        151bp
        381.5M
        659_cTi-T1-TRNA-1_B23WHTKLT4_1_2
        88.6%
        54%
        151bp
        381.5M
        659_cTi-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.5%
        54%
        137bp
        294.9M
        88.5%
        54%
        137bp
        294.9M
        659_cTi-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.5%
        55%
        137bp
        294.9M
        88.5%
        55%
        137bp
        294.9M
        659_cVw-T1-TRNA-1_B23WHTKLT4_1
        166.8bp
        150.0bp
        0.01%
        1.20%
        0.47%
        0.00%
        0.01%
        3.92%
        0.75%
        0.00%
        5.16
        9999000000
        137360432
        No
        0.00%
        591101048
        0.01%
        0.71%
        168516460
        0.91%
        0.95%
        0.07%
        0.05%
        154093599
        78252340
        11102695
        3706285
        318.47
        132.00
        0.69%
        0.55%
        0.97
        0.000
        569212480
        0.8984
        71.9%
        88.9%
        95.5%
        282.3M
        53.6%
        591.1M
        56.7%
        98.5%
        43.9%
        659_cVw-T1-TRNA-1_B23WHTKLT4_1_1
        166.8bp
        98.0%
        289.8M
        89.5%
        55%
        151bp
        379.3M
        659_cVw-T1-TRNA-1_B23WHTKLT4_1_2
        88.1%
        56%
        151bp
        379.3M
        659_cVw-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.1%
        56%
        151bp
        295.6M
        88.1%
        56%
        151bp
        295.6M
        659_cVw-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.9%
        56%
        151bp
        295.6M
        87.9%
        56%
        151bp
        295.6M
        659_cWx-T1-TRNA-1_B23WHTKLT4_1
        152.0bp
        137.0bp
        0.01%
        1.19%
        0.43%
        0.00%
        0.01%
        3.77%
        0.71%
        0.00%
        0.00
        9999000000
        63737409
        No
        0.00%
        585762234
        0.01%
        0.89%
        64828542
        0.83%
        0.90%
        0.12%
        0.10%
        53709489
        27145306
        7748184
        3064844
        97.38
        30.00
        0.50%
        0.25%
        0.97
        0.000
        563277831
        0.9268
        89.4%
        87.5%
        95.2%
        278.9M
        65.0%
        585.8M
        60.0%
        97.9%
        52.6%
        659_cWx-T1-TRNA-1_B23WHTKLT4_1_1
        152.0bp
        97.5%
        285.6M
        92.0%
        58%
        151bp
        299.1M
        659_cWx-T1-TRNA-1_B23WHTKLT4_1_2
        90.1%
        59%
        151bp
        299.1M
        659_cWx-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        92.1%
        59%
        147bp
        292.9M
        92.1%
        59%
        147bp
        292.9M
        659_cWx-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        91.7%
        60%
        147bp
        292.9M
        91.7%
        60%
        147bp
        292.9M
        659_cfy-T1-TRNA-1_B23WHTKLT4_1
        152.5bp
        139.0bp
        0.01%
        1.01%
        0.65%
        0.00%
        0.02%
        3.08%
        0.94%
        0.00%
        0.01
        9999000000
        143657848
        No
        0.00%
        591305148
        0.00%
        0.71%
        172514574
        0.93%
        0.96%
        0.06%
        0.04%
        160158060
        81091728
        9979754
        3418561
        311.84
        118.00
        0.67%
        0.53%
        0.97
        0.000
        576767058
        0.9002
        71.1%
        91.8%
        96.6%
        285.5M
        51.5%
        591.3M
        56.4%
        98.6%
        52.4%
        659_cfy-T1-TRNA-1_B23WHTKLT4_1_1
        152.5bp
        98.4%
        290.9M
        89.4%
        55%
        151bp
        412.3M
        659_cfy-T1-TRNA-1_B23WHTKLT4_1_2
        88.5%
        56%
        151bp
        412.3M
        659_cfy-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        87.4%
        56%
        147bp
        295.7M
        87.4%
        56%
        147bp
        295.7M
        659_cfy-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.7%
        56%
        147bp
        295.7M
        87.7%
        56%
        147bp
        295.7M
        659_ciJ-T1-TRNA-1_B23WHTKLT4_1
        161.7bp
        146.0bp
        0.00%
        0.86%
        0.40%
        0.00%
        0.01%
        2.69%
        0.61%
        0.00%
        0.01
        9999000000
        113263310
        No
        0.00%
        570791160
        0.01%
        0.74%
        146509508
        0.90%
        0.95%
        0.07%
        0.05%
        132441230
        67339567
        10756730
        3681257
        260.22
        112.00
        0.69%
        0.52%
        0.97
        0.000
        553377113
        0.8975
        74.8%
        90.5%
        96.0%
        274.1M
        55.1%
        570.8M
        57.2%
        98.5%
        46.6%
        659_ciJ-T1-TRNA-1_B23WHTKLT4_1_1
        161.7bp
        97.6%
        278.7M
        87.7%
        56%
        151bp
        289.8M
        659_ciJ-T1-TRNA-1_B23WHTKLT4_1_2
        86.6%
        57%
        151bp
        289.8M
        659_ciJ-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        87.8%
        56%
        151bp
        285.4M
        87.8%
        56%
        151bp
        285.4M
        659_ciJ-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.8%
        57%
        151bp
        285.4M
        87.8%
        57%
        151bp
        285.4M
        659_ciZ-T1-TRNA-1_B23WHTKLT4_1
        124.2bp
        116.0bp
        0.01%
        1.81%
        0.69%
        0.00%
        0.01%
        5.17%
        1.03%
        0.00%
        4.47
        9999000000
        79373975
        No
        0.00%
        590953388
        0.00%
        0.85%
        86431534
        0.87%
        0.91%
        0.10%
        0.09%
        75204389
        37842082
        8363482
        3593935
        117.40
        45.00
        0.57%
        0.32%
        0.98
        0.000
        570424517
        0.9030
        85.8%
        88.6%
        95.4%
        281.9M
        54.0%
        591.0M
        54.7%
        98.5%
        74.1%
        659_ciZ-T1-TRNA-1_B23WHTKLT4_1_1
        124.2bp
        98.3%
        290.4M
        90.3%
        53%
        151bp
        318.6M
        659_ciZ-T1-TRNA-1_B23WHTKLT4_1_2
        89.0%
        54%
        151bp
        318.6M
        659_ciZ-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        90.0%
        54%
        122bp
        295.5M
        90.0%
        54%
        122bp
        295.5M
        659_ciZ-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.8%
        54%
        122bp
        295.5M
        89.8%
        54%
        122bp
        295.5M
        659_d01-T1-TRNA-1_B23WHTKLT4_1
        143.1bp
        130.0bp
        0.00%
        1.01%
        0.36%
        0.00%
        0.01%
        3.26%
        0.57%
        0.00%
        0.40
        9999000000
        96219317
        No
        0.00%
        488765340
        0.01%
        0.80%
        95337570
        0.87%
        0.92%
        0.10%
        0.08%
        83415257
        42157238
        9265223
        3736433
        143.96
        68.00
        0.63%
        0.42%
        0.97
        0.000
        472267925
        0.9240
        80.9%
        89.3%
        95.8%
        234.0M
        55.5%
        488.8M
        55.9%
        98.1%
        58.6%
        659_d01-T1-TRNA-1_B23WHTKLT4_1_1
        143.1bp
        97.5%
        238.3M
        87.7%
        55%
        151bp
        249.1M
        659_d01-T1-TRNA-1_B23WHTKLT4_1_2
        86.4%
        55%
        151bp
        249.1M
        659_d01-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        87.8%
        55%
        137bp
        244.4M
        87.8%
        55%
        137bp
        244.4M
        659_d01-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.8%
        56%
        137bp
        244.4M
        87.8%
        56%
        137bp
        244.4M
        659_dES-T1-TRNA-1_B23WHTKLT4_1
        146.3bp
        135.0bp
        0.01%
        1.31%
        0.38%
        0.00%
        0.01%
        4.14%
        0.65%
        0.00%
        0.02
        9999000000
        123477522
        No
        0.00%
        592228976
        0.01%
        0.76%
        139785128
        0.89%
        0.93%
        0.08%
        0.07%
        124994666
        63184180
        11643606
        4706129
        228.13
        103.00
        0.67%
        0.51%
        0.98
        0.000
        572226675
        0.8814
        76.8%
        89.0%
        95.7%
        283.2M
        55.1%
        592.2M
        53.7%
        98.7%
        57.1%
        659_dES-T1-TRNA-1_B23WHTKLT4_1_1
        146.3bp
        98.0%
        290.3M
        88.6%
        52%
        151bp
        331.1M
        659_dES-T1-TRNA-1_B23WHTKLT4_1_2
        87.9%
        53%
        151bp
        331.1M
        659_dES-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.0%
        53%
        142bp
        296.1M
        88.0%
        53%
        142bp
        296.1M
        659_dES-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.2%
        53%
        142bp
        296.1M
        88.2%
        53%
        142bp
        296.1M
        659_dc8-T1-TRNA-1_B23WHTKLT4_1
        152.9bp
        137.0bp
        0.01%
        1.63%
        0.47%
        0.00%
        0.02%
        4.92%
        0.74%
        0.00%
        5.03
        9999000000
        93998467
        No
        0.00%
        588750708
        0.01%
        0.83%
        101432330
        0.88%
        0.93%
        0.09%
        0.07%
        89078926
        45145507
        9368034
        3550037
        166.78
        74.00
        0.64%
        0.44%
        0.97
        0.000
        569278483
        0.9457
        83.2%
        89.5%
        95.7%
        281.8M
        60.3%
        588.8M
        56.0%
        98.1%
        51.7%
        659_dc8-T1-TRNA-1_B23WHTKLT4_1_1
        152.9bp
        97.7%
        287.6M
        90.2%
        55%
        151bp
        312.2M
        659_dc8-T1-TRNA-1_B23WHTKLT4_1_2
        88.8%
        55%
        151bp
        312.2M
        659_dc8-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        90.1%
        55%
        147bp
        294.4M
        90.1%
        55%
        147bp
        294.4M
        659_dc8-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        90.0%
        56%
        147bp
        294.4M
        90.0%
        56%
        147bp
        294.4M
        659_dcz-T1-TRNA-1_B23WHTKLT4_1
        118.0bp
        112.0bp
        0.01%
        1.22%
        0.27%
        0.00%
        0.01%
        3.74%
        0.47%
        0.00%
        6.26
        9999000000
        15869757
        No
        0.00%
        95842054
        0.01%
        0.88%
        11541198
        0.72%
        0.78%
        0.21%
        0.22%
        8355873
        4188498
        2435400
        1157885
        11.41
        4.00
        0.09%
        0.01%
        0.98
        0.000
        91498544
        0.8360
        88.7%
        85.1%
        94.3%
        45.2M
        53.1%
        95.8M
        55.3%
        97.5%
        80.9%
        659_dcz-T1-TRNA-1_B23WHTKLT4_1_1
        118.0bp
        96.6%
        46.3M
        87.4%
        54%
        151bp
        49.2M
        659_dcz-T1-TRNA-1_B23WHTKLT4_1_2
        86.0%
        55%
        151bp
        49.2M
        659_dcz-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        87.3%
        55%
        112bp
        47.9M
        87.3%
        55%
        112bp
        47.9M
        659_dcz-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        87.0%
        55%
        117bp
        47.9M
        87.0%
        55%
        117bp
        47.9M
        659_djb-T1-TRNA-1_B23WHTKLT4_1
        140.5bp
        131.0bp
        0.01%
        1.72%
        0.56%
        0.00%
        0.02%
        5.20%
        0.89%
        0.00%
        0.02
        9999000000
        101248494
        No
        0.00%
        591967586
        0.01%
        0.80%
        117243144
        0.89%
        0.92%
        0.09%
        0.08%
        104025136
        52464366
        10254761
        4285392
        178.98
        69.00
        0.62%
        0.43%
        0.98
        0.000
        574663767
        0.8843
        80.6%
        90.1%
        96.0%
        284.2M
        57.7%
        592.0M
        54.3%
        98.7%
        61.8%
        659_djb-T1-TRNA-1_B23WHTKLT4_1_1
        140.5bp
        98.1%
        290.4M
        88.4%
        53%
        151bp
        301.9M
        659_djb-T1-TRNA-1_B23WHTKLT4_1_2
        87.5%
        54%
        151bp
        301.9M
        659_djb-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.5%
        54%
        137bp
        296.0M
        88.5%
        54%
        137bp
        296.0M
        659_djb-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.6%
        54%
        137bp
        296.0M
        88.6%
        54%
        137bp
        296.0M
        659_dm4-T1-TRNA-1_B23WHTKLT4_1
        119.7bp
        113.0bp
        0.01%
        1.25%
        0.28%
        0.00%
        0.02%
        3.80%
        0.48%
        0.00%
        6.19
        9999000000
        29157083
        No
        0.00%
        204320002
        0.01%
        0.89%
        22896872
        0.76%
        0.82%
        0.18%
        0.18%
        17368714
        8712257
        4215978
        1968126
        25.40
        10.00
        0.25%
        0.04%
        0.98
        0.000
        195371226
        0.8965
        89.4%
        85.5%
        94.6%
        96.6M
        54.5%
        204.3M
        55.8%
        98.0%
        77.8%
        659_dm4-T1-TRNA-1_B23WHTKLT4_1_1
        119.7bp
        97.2%
        99.3M
        89.0%
        55%
        151bp
        104.2M
        659_dm4-T1-TRNA-1_B23WHTKLT4_1_2
        87.5%
        55%
        151bp
        104.2M
        659_dm4-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.0%
        55%
        117bp
        102.2M
        89.0%
        55%
        117bp
        102.2M
        659_dm4-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.6%
        55%
        117bp
        102.2M
        88.6%
        55%
        117bp
        102.2M
        659_dts-T1-TRNA-1_B23WHTKLT4_1
        133.9bp
        122.0bp
        0.00%
        0.90%
        0.46%
        0.00%
        0.01%
        2.82%
        0.71%
        0.00%
        6.12
        9999000000
        83343473
        No
        0.00%
        411607076
        0.01%
        0.77%
        96486554
        0.90%
        0.94%
        0.08%
        0.06%
        86844109
        43784111
        7247690
        2884615
        147.16
        68.00
        0.64%
        0.41%
        0.97
        0.000
        397621721
        0.9155
        77.0%
        89.0%
        95.5%
        196.6M
        50.1%
        411.6M
        55.9%
        98.3%
        65.0%
        659_dts-T1-TRNA-1_B23WHTKLT4_1_1
        133.9bp
        98.1%
        201.9M
        86.6%
        54%
        151bp
        209.5M
        659_dts-T1-TRNA-1_B23WHTKLT4_1_2
        85.3%
        55%
        151bp
        209.5M
        659_dts-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        86.6%
        55%
        127bp
        205.8M
        86.6%
        55%
        127bp
        205.8M
        659_dts-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        86.6%
        56%
        127bp
        205.8M
        86.6%
        56%
        127bp
        205.8M
        659_ecd-T1-TRNA-1_B23WHTKLT4_1
        166.5bp
        150.0bp
        0.01%
        0.79%
        0.35%
        0.00%
        0.01%
        2.53%
        0.54%
        0.00%
        6.13
        9999000000
        189426589
        No
        0.00%
        592213586
        0.00%
        0.63%
        216859066
        0.93%
        0.96%
        0.06%
        0.04%
        201451090
        102365147
        12927003
        4172367
        419.26
        187.00
        0.73%
        0.60%
        0.97
        0.000
        575965912
        0.9162
        63.6%
        91.0%
        96.4%
        285.3M
        46.5%
        592.2M
        54.9%
        98.7%
        44.2%
        659_ecd-T1-TRNA-1_B23WHTKLT4_1_1
        166.5bp
        98.1%
        290.4M
        86.6%
        54%
        151bp
        334.2M
        659_ecd-T1-TRNA-1_B23WHTKLT4_1_2
        86.0%
        54%
        151bp
        334.2M
        659_ecd-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        85.8%
        54%
        151bp
        296.1M
        85.8%
        54%
        151bp
        296.1M
        659_ecd-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        86.2%
        55%
        151bp
        296.1M
        86.2%
        55%
        151bp
        296.1M
        659_epP-T1-TRNA-1_B23WHTKLT4_1
        151.5bp
        138.0bp
        0.00%
        0.76%
        0.36%
        0.00%
        0.01%
        2.47%
        0.58%
        0.00%
        0.00
        9999000000
        90359821
        No
        0.00%
        590659730
        0.01%
        0.83%
        100251628
        0.88%
        0.93%
        0.09%
        0.07%
        88112494
        44641405
        8870408
        3391829
        163.45
        68.00
        0.62%
        0.42%
        0.97
        0.000
        566230409
        0.8984
        83.5%
        86.9%
        94.9%
        280.3M
        61.2%
        590.7M
        57.5%
        98.4%
        53.1%
        659_epP-T1-TRNA-1_B23WHTKLT4_1_1
        151.5bp
        97.8%
        288.9M
        90.2%
        56%
        151bp
        326.1M
        659_epP-T1-TRNA-1_B23WHTKLT4_1_2
        89.0%
        57%
        151bp
        326.1M
        659_epP-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        89.8%
        57%
        147bp
        295.3M
        89.8%
        57%
        147bp
        295.3M
        659_epP-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        89.7%
        57%
        147bp
        295.3M
        89.7%
        57%
        147bp
        295.3M
        659_ezg-T1-TRNA-1_B23WHTKLT4_1
        149.5bp
        137.0bp
        0.01%
        1.56%
        0.61%
        0.00%
        0.01%
        4.74%
        0.93%
        0.00%
        0.00
        9999000000
        117248628
        No
        0.00%
        591672028
        0.01%
        0.76%
        140553668
        0.91%
        0.94%
        0.07%
        0.06%
        127389630
        64476531
        10093868
        3791091
        237.28
        99.00
        0.66%
        0.50%
        0.97
        0.000
        573028518
        0.9160
        76.6%
        89.4%
        95.8%
        283.6M
        55.8%
        591.7M
        55.9%
        98.6%
        55.0%
        659_ezg-T1-TRNA-1_B23WHTKLT4_1_1
        149.5bp
        97.9%
        289.5M
        90.1%
        55%
        151bp
        403.5M
        659_ezg-T1-TRNA-1_B23WHTKLT4_1_2
        89.1%
        55%
        151bp
        403.5M
        659_ezg-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        88.3%
        55%
        142bp
        295.8M
        88.3%
        55%
        142bp
        295.8M
        659_ezg-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        88.4%
        56%
        142bp
        295.8M
        88.4%
        56%
        142bp
        295.8M
        659_gK-T1-TRNA-1_B23WHTKLT4_1
        158.5bp
        143.0bp
        0.01%
        1.44%
        0.48%
        0.00%
        0.02%
        4.51%
        0.77%
        0.00%
        0.01
        9999000000
        74879347
        No
        0.00%
        370530330
        0.00%
        0.74%
        94660136
        0.91%
        0.95%
        0.07%
        0.05%
        85933238
        43603839
        6959788
        2438904
        167.46
        63.00
        0.60%
        0.42%
        0.97
        0.000
        355852652
        0.9378
        74.8%
        88.3%
        95.3%
        176.5M
        53.9%
        370.5M
        55.2%
        98.3%
        48.8%
        659_gK-T1-TRNA-1_B23WHTKLT4_1_1
        158.5bp
        98.0%
        181.6M
        86.6%
        54%
        151bp
        188.5M
        659_gK-T1-TRNA-1_B23WHTKLT4_1_2
        85.3%
        55%
        151bp
        188.5M
        659_gK-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        86.6%
        54%
        151bp
        185.3M
        86.6%
        54%
        151bp
        185.3M
        659_gK-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        86.6%
        55%
        151bp
        185.3M
        86.6%
        55%
        151bp
        185.3M
        659_rd-T1-TRNA-1_B23WHTKLT4_1
        166.9bp
        151.0bp
        0.00%
        1.15%
        0.29%
        0.00%
        0.00%
        3.94%
        0.49%
        0.00%
        6.58
        9999000000
        161387016
        No
        0.00%
        591807916
        0.00%
        0.67%
        192797695
        0.92%
        0.96%
        0.06%
        0.04%
        177573775
        90230562
        12513618
        4182124
        368.11
        151.00
        0.71%
        0.57%
        0.97
        0.000
        578530795
        0.9177
        67.7%
        92.2%
        96.7%
        286.0M
        50.5%
        591.8M
        54.7%
        98.6%
        43.8%
        659_rd-T1-TRNA-1_B23WHTKLT4_1_1
        166.9bp
        98.0%
        290.0M
        87.6%
        54%
        151bp
        342.6M
        659_rd-T1-TRNA-1_B23WHTKLT4_1_2
        86.7%
        54%
        151bp
        342.6M
        659_rd-T1-TRNA-1_B23WHTKLT4_1_trimmed_1
        86.7%
        54%
        151bp
        295.9M
        86.7%
        54%
        151bp
        295.9M
        659_rd-T1-TRNA-1_B23WHTKLT4_1_trimmed_2
        86.9%
        54%
        151bp
        295.9M
        86.9%
        54%
        151bp
        295.9M
        FFPE_HD789_01_RNA_13_B23WHTKLT4_1
        178.1bp
        162.0bp
        0.01%
        1.66%
        0.70%
        0.00%
        0.01%
        4.82%
        0.98%
        0.00%
        3.97
        9999000000
        540326763
        Yes
        62.47%
        592410540
        0.00%
        0.40%
        354735120
        0.95%
        0.98%
        0.05%
        0.02%
        336060150
        170826735
        16008505
        4322675
        755.01
        306.00
        0.72%
        0.63%
        0.97
        0.000
        575712631
        0.9419
        40.3%
        91.2%
        96.5%
        285.8M
        29.8%
        592.4M
        54.8%
        98.7%
        37.6%
        FFPE_HD789_01_RNA_13_B23WHTKLT4_1_1
        178.1bp
        98.1%
        290.7M
        87.3%
        54%
        151bp
        519.2M
        FFPE_HD789_01_RNA_13_B23WHTKLT4_1_2
        86.8%
        54%
        151bp
        519.2M
        FFPE_HD789_01_RNA_13_B23WHTKLT4_1_trimmed_1
        83.9%
        54%
        151bp
        296.2M
        83.9%
        54%
        151bp
        296.2M
        FFPE_HD789_01_RNA_13_B23WHTKLT4_1_trimmed_2
        84.6%
        55%
        151bp
        296.2M
        84.6%
        55%
        151bp
        296.2M
        FFPE_V4_0002_RNA_0001_B23WHTKLT4_1
        164.5bp
        150.0bp
        0.01%
        1.69%
        1.04%
        0.00%
        0.01%
        4.66%
        1.45%
        0.00%
        6.67
        9999000000
        287128417
        No
        0.00%
        591836756
        0.01%
        0.52%
        282328251
        0.93%
        0.96%
        0.06%
        0.04%
        262889947
        133662307
        15753484
        4930012
        550.34
        136.00
        0.60%
        0.52%
        0.97
        0.000
        571781095
        0.9455
        52.6%
        89.4%
        95.8%
        283.4M
        37.8%
        591.8M
        55.1%
        98.6%
        44.9%
        FFPE_V4_0002_RNA_0001_B23WHTKLT4_1_1
        164.5bp
        96.9%
        286.8M
        85.7%
        54%
        151bp
        318.2M
        FFPE_V4_0002_RNA_0001_B23WHTKLT4_1_2
        85.2%
        55%
        151bp
        318.2M
        FFPE_V4_0002_RNA_0001_B23WHTKLT4_1_trimmed_1
        85.4%
        54%
        151bp
        295.9M
        85.4%
        54%
        151bp
        295.9M
        FFPE_V4_0002_RNA_0001_B23WHTKLT4_1_trimmed_2
        85.9%
        55%
        151bp
        295.9M
        85.9%
        55%
        151bp
        295.9M
        NTC_0001_0001_B23WHTKLT4_1
        90.2bp
        31.0bp
        0.00%
        0.00%
        0.04%
        0.00%
        0.00%
        0.00%
        0.08%
        0.00%
        0.00
        9999000000
        48139
        Skipped
        N/A%
        53950
        0.57%
        0.26%
        40048
        0.01%
        0.03%
        0.22%
        0.97%
        257
        135
        8828
        4270
        0.00
        0.00
        0.00%
        0.00%
        0.89
        0.000
        7369
        0.4305
        60.5%
        3.0%
        10.0%
        0.0M
        67.3%
        0.1M
        66.5%
        3.2%
        50.6%
        NTC_0001_0001_B23WHTKLT4_1_1
        90.2bp
        10.7%
        0.0M
        98.1%
        73%
        151bp
        0.8M
        NTC_0001_0001_B23WHTKLT4_1_2
        97.0%
        98%
        151bp
        0.8M
        NTC_0001_0001_B23WHTKLT4_1_trimmed_1
        58.6%
        62%
        112bp
        0.0M
        58.6%
        62%
        112bp
        0.0M
        NTC_0001_0001_B23WHTKLT4_1_trimmed_2
        61.7%
        69%
        112bp
        0.0M
        61.7%
        69%
        112bp
        0.0M
        tih_rna_sample_00087_B23WHTKLT4_1
        156.0bp
        142.0bp
        0.01%
        1.55%
        0.58%
        0.00%
        0.01%
        4.81%
        0.85%
        0.00%
        0.01
        9999000000
        250322595
        No
        0.00%
        592566674
        0.00%
        0.55%
        267623464
        0.93%
        0.96%
        0.06%
        0.04%
        249512559
        126439077
        15253419
        5326738
        499.38
        217.00
        0.70%
        0.60%
        0.97
        0.000
        572769843
        0.9357
        55.1%
        89.5%
        95.8%
        283.8M
        39.4%
        592.6M
        54.2%
        98.8%
        49.8%
        tih_rna_sample_00087_B23WHTKLT4_1_1
        156.0bp
        98.1%
        290.5M
        87.8%
        53%
        151bp
        497.8M
        tih_rna_sample_00087_B23WHTKLT4_1_2
        87.3%
        54%
        151bp
        497.8M
        tih_rna_sample_00087_B23WHTKLT4_1_trimmed_1
        84.2%
        53%
        151bp
        296.3M
        84.2%
        53%
        151bp
        296.3M
        tih_rna_sample_00087_B23WHTKLT4_1_trimmed_2
        84.7%
        54%
        151bp
        296.3M
        84.7%
        54%
        151bp
        296.3M
        tih_rna_sample_00087_B23WHTKLT4_2
        149.6bp
        137.0bp
        0.01%
        1.60%
        0.60%
        0.00%
        0.01%
        4.90%
        0.87%
        0.00%
        0.01
        9999000000
        437587943
        No
        0.00%
        592124460
        0.01%
        0.46%
        318842189
        0.94%
        0.96%
        0.05%
        0.04%
        298741381
        151171037
        17135281
        6053457
        587.61
        262.00
        0.71%
        0.62%
        0.97
        0.000
        571488309
        0.9467
        46.4%
        89.1%
        95.7%
        283.2M
        30.1%
        592.1M
        54.0%
        98.7%
        54.0%
        tih_rna_sample_00087_B23WHTKLT4_2_1
        149.6bp
        98.0%
        290.2M
        86.0%
        53%
        151bp
        441.5M
        tih_rna_sample_00087_B23WHTKLT4_2_2
        85.3%
        54%
        151bp
        441.5M
        tih_rna_sample_00087_B23WHTKLT4_2_trimmed_1
        83.3%
        53%
        142bp
        296.1M
        83.3%
        53%
        142bp
        296.1M
        tih_rna_sample_00087_B23WHTKLT4_2_trimmed_2
        83.6%
        54%
        142bp
        296.1M
        83.6%
        54%
        142bp
        296.1M
        tih_rna_sample_00089_B23WHTKLT4_1
        152.4bp
        139.0bp
        0.01%
        1.38%
        0.55%
        0.00%
        0.02%
        4.26%
        0.84%
        0.00%
        3.80
        9999000000
        133957964
        No
        0.00%
        369624532
        0.00%
        0.58%
        153613084
        0.94%
        0.96%
        0.05%
        0.04%
        143850196
        72847735
        8196218
        2775175
        281.91
        120.00
        0.67%
        0.53%
        0.97
        0.000
        357883114
        0.9326
        58.7%
        89.2%
        95.9%
        177.3M
        39.1%
        369.6M
        56.1%
        98.7%
        52.3%
        tih_rna_sample_00089_B23WHTKLT4_1_1
        152.4bp
        98.2%
        181.6M
        82.7%
        55%
        151bp
        187.3M
        tih_rna_sample_00089_B23WHTKLT4_1_2
        82.3%
        55%
        151bp
        187.3M
        tih_rna_sample_00089_B23WHTKLT4_1_trimmed_1
        82.7%
        55%
        147bp
        184.8M
        82.7%
        55%
        147bp
        184.8M
        tih_rna_sample_00089_B23WHTKLT4_1_trimmed_2
        83.2%
        56%
        147bp
        184.8M
        83.2%
        56%
        147bp
        184.8M
        tih_rna_sample_00089_B23WHTKLT4_2
        152.3bp
        138.0bp
        0.01%
        1.41%
        0.56%
        0.00%
        0.02%
        4.32%
        0.85%
        0.00%
        3.87
        9999000000
        247812145
        No
        0.00%
        591867468
        0.00%
        0.55%
        263912418
        0.94%
        0.96%
        0.05%
        0.04%
        247428606
        125318039
        13828301
        4625631
        487.88
        219.00
        0.72%
        0.61%
        0.97
        0.000
        572773789
        0.9284
        55.7%
        89.2%
        95.9%
        283.8M
        39.0%
        591.9M
        56.0%
        98.6%
        52.1%
        tih_rna_sample_00089_B23WHTKLT4_2_1
        152.3bp
        98.2%
        290.5M
        86.1%
        55%
        151bp
        364.2M
        tih_rna_sample_00089_B23WHTKLT4_2_2
        85.5%
        55%
        151bp
        364.2M
        tih_rna_sample_00089_B23WHTKLT4_2_trimmed_1
        84.7%
        55%
        147bp
        295.9M
        84.7%
        55%
        147bp
        295.9M
        tih_rna_sample_00089_B23WHTKLT4_2_trimmed_2
        85.1%
        56%
        147bp
        295.9M
        85.1%
        56%
        147bp
        295.9M
        tih_rna_sample_00090_B23WHTKLT4_1
        125.3bp
        118.0bp
        0.00%
        1.23%
        0.55%
        0.00%
        0.01%
        3.56%
        0.85%
        0.00%
        5.59
        9999000000
        66987932
        No
        0.00%
        417753942
        0.01%
        0.83%
        72574199
        0.87%
        0.92%
        0.09%
        0.08%
        62945929
        31683062
        6854079
        2916928
        100.93
        23.00
        0.46%
        0.25%
        0.97
        0.000
        397260774
        0.9188
        83.2%
        83.9%
        94.1%
        196.5M
        51.1%
        417.8M
        60.2%
        98.3%
        72.0%
        tih_rna_sample_00090_B23WHTKLT4_1_1
        125.3bp
        97.6%
        203.9M
        88.5%
        58%
        151bp
        212.6M
        tih_rna_sample_00090_B23WHTKLT4_1_2
        87.2%
        59%
        151bp
        212.6M
        tih_rna_sample_00090_B23WHTKLT4_1_trimmed_1
        88.6%
        60%
        122bp
        208.9M
        88.6%
        60%
        122bp
        208.9M
        tih_rna_sample_00090_B23WHTKLT4_1_trimmed_2
        88.4%
        60%
        122bp
        208.9M
        88.4%
        60%
        122bp
        208.9M
        tih_rna_sample_00090_B23WHTKLT4_2
        128.5bp
        120.0bp
        0.01%
        1.25%
        0.56%
        0.00%
        0.01%
        3.60%
        0.85%
        0.00%
        5.65
        9999000000
        104269065
        No
        0.00%
        588771970
        0.01%
        0.79%
        121506860
        0.89%
        0.93%
        0.08%
        0.07%
        107819132
        54241234
        9767519
        4035728
        172.44
        44.00
        0.55%
        0.36%
        0.97
        0.000
        560633905
        0.9500
        79.9%
        84.5%
        94.2%
        277.3M
        51.2%
        588.8M
        59.7%
        98.1%
        69.5%
        tih_rna_sample_00090_B23WHTKLT4_2_1
        128.5bp
        97.8%
        287.8M
        89.3%
        58%
        151bp
        334.5M
        tih_rna_sample_00090_B23WHTKLT4_2_2
        88.1%
        58%
        151bp
        334.5M
        tih_rna_sample_00090_B23WHTKLT4_2_trimmed_1
        88.7%
        59%
        127bp
        294.4M
        88.7%
        59%
        127bp
        294.4M
        tih_rna_sample_00090_B23WHTKLT4_2_trimmed_2
        88.8%
        59%
        127bp
        294.4M
        88.8%
        59%
        127bp
        294.4M
        tih_rna_sample_00096_B23WHTKLT4_1
        153.6bp
        140.0bp
        0.01%
        0.70%
        0.33%
        0.00%
        0.02%
        2.18%
        0.52%
        0.00%
        0.01
        9999000000
        158211757
        No
        0.00%
        591755752
        0.00%
        0.68%
        190270913
        0.92%
        0.95%
        0.06%
        0.05%
        175190085
        88795564
        12260018
        4367465
        337.00
        159.00
        0.71%
        0.58%
        0.98
        0.000
        574910348
        0.9245
        68.2%
        90.7%
        96.3%
        284.9M
        49.0%
        591.8M
        55.0%
        98.6%
        52.3%
        tih_rna_sample_00096_B23WHTKLT4_1_1
        153.6bp
        98.0%
        290.0M
        86.6%
        54%
        151bp
        331.9M
        tih_rna_sample_00096_B23WHTKLT4_1_2
        85.9%
        54%
        151bp
        331.9M
        tih_rna_sample_00096_B23WHTKLT4_1_trimmed_1
        85.8%
        54%
        147bp
        295.9M
        85.8%
        54%
        147bp
        295.9M
        tih_rna_sample_00096_B23WHTKLT4_1_trimmed_2
        86.1%
        55%
        147bp
        295.9M
        86.1%
        55%
        147bp
        295.9M
        tih_rna_sample_00096_B23WHTKLT4_2
        149.8bp
        138.0bp
        0.01%
        0.72%
        0.34%
        0.00%
        0.02%
        2.24%
        0.53%
        0.00%
        0.02
        9999000000
        223085521
        No
        0.00%
        591655010
        0.00%
        0.57%
        252351041
        0.93%
        0.96%
        0.06%
        0.04%
        235397793
        119189948
        14075392
        4904256
        455.37
        224.00
        0.74%
        0.62%
        0.97
        0.000
        574634680
        0.9416
        57.6%
        90.7%
        96.3%
        284.8M
        39.7%
        591.7M
        54.7%
        98.6%
        53.9%
        tih_rna_sample_00096_B23WHTKLT4_2_1
        149.8bp
        98.1%
        290.1M
        86.4%
        53%
        151bp
        400.2M
        tih_rna_sample_00096_B23WHTKLT4_2_2
        85.8%
        54%
        151bp
        400.2M
        tih_rna_sample_00096_B23WHTKLT4_2_trimmed_1
        84.2%
        54%
        142bp
        295.8M
        84.2%
        54%
        142bp
        295.8M
        tih_rna_sample_00096_B23WHTKLT4_2_trimmed_2
        84.7%
        54%
        147bp
        295.8M
        84.7%
        54%
        147bp
        295.8M
        tih_rna_sample_00097_B23WHTKLT4_1
        133.6bp
        124.0bp
        0.01%
        2.34%
        0.75%
        0.00%
        0.01%
        6.30%
        1.09%
        0.00%
        0.01
        9999000000
        116809840
        No
        0.00%
        592101470
        0.00%
        0.77%
        138094829
        0.92%
        0.95%
        0.07%
        0.05%
        126441387
        63788457
        9108404
        3580974
        215.58
        81.00
        0.63%
        0.46%
        0.98
        0.000
        572492236
        0.9134
        77.0%
        88.9%
        95.7%
        283.2M
        50.7%
        592.1M
        53.6%
        98.7%
        67.2%
        tih_rna_sample_00097_B23WHTKLT4_1_1
        133.6bp
        98.3%
        291.1M
        90.4%
        53%
        151bp
        410.0M
        tih_rna_sample_00097_B23WHTKLT4_1_2
        89.5%
        53%
        151bp
        410.0M
        tih_rna_sample_00097_B23WHTKLT4_1_trimmed_1
        88.5%
        53%
        127bp
        296.1M
        88.5%
        53%
        127bp
        296.1M
        tih_rna_sample_00097_B23WHTKLT4_1_trimmed_2
        88.7%
        53%
        127bp
        296.1M
        88.7%
        53%
        127bp
        296.1M
        tih_rna_sample_00097_B23WHTKLT4_2
        128.7bp
        119.0bp
        0.01%
        2.45%
        0.77%
        0.00%
        0.02%
        6.53%
        1.12%
        0.00%
        0.00
        9999000000
        134832103
        No
        0.00%
        590573458
        0.00%
        0.70%
        175553039
        0.93%
        0.95%
        0.06%
        0.05%
        162677752
        81969454
        10233714
        3946478
        273.65
        107.00
        0.66%
        0.51%
        0.98
        0.000
        570176005
        0.9190
        70.6%
        88.6%
        95.5%
        282.0M
        44.5%
        590.6M
        53.3%
        98.4%
        69.6%
        tih_rna_sample_00097_B23WHTKLT4_2_1
        128.7bp
        98.4%
        290.5M
        87.9%
        52%
        151bp
        339.3M
        tih_rna_sample_00097_B23WHTKLT4_2_2
        86.7%
        53%
        151bp
        339.3M
        tih_rna_sample_00097_B23WHTKLT4_2_trimmed_1
        87.0%
        53%
        122bp
        295.3M
        87.0%
        53%
        122bp
        295.3M
        tih_rna_sample_00097_B23WHTKLT4_2_trimmed_2
        87.1%
        53%
        122bp
        295.3M
        87.1%
        53%
        122bp
        295.3M
        tih_rna_sample_00116_B23WHTKLT4_1
        129.4bp
        122.0bp
        0.01%
        1.32%
        0.69%
        0.00%
        0.01%
        3.71%
        0.98%
        0.00%
        4.99
        9999000000
        88617647
        No
        0.00%
        591507502
        0.01%
        0.84%
        92863676
        0.87%
        0.92%
        0.09%
        0.08%
        81127545
        40852172
        8362235
        3533247
        131.01
        49.00
        0.59%
        0.34%
        0.97
        0.000
        573704433
        0.9194
        84.8%
        89.7%
        95.8%
        283.3M
        56.0%
        591.5M
        56.4%
        98.6%
        70.5%
        tih_rna_sample_00116_B23WHTKLT4_1_1
        129.4bp
        97.8%
        289.2M
        90.3%
        55%
        151bp
        318.8M
        tih_rna_sample_00116_B23WHTKLT4_1_2
        89.1%
        56%
        151bp
        318.8M
        tih_rna_sample_00116_B23WHTKLT4_1_trimmed_1
        90.0%
        56%
        127bp
        295.8M
        90.0%
        56%
        127bp
        295.8M
        tih_rna_sample_00116_B23WHTKLT4_1_trimmed_2
        89.9%
        56%
        127bp
        295.8M
        89.9%
        56%
        127bp
        295.8M
        tih_rna_sample_00116_B23WHTKLT4_2
        127.2bp
        119.0bp
        0.01%
        1.36%
        0.71%
        0.00%
        0.01%
        3.83%
        1.00%
        0.00%
        5.02
        9999000000
        119689967
        No
        0.00%
        558892094
        0.01%
        0.75%
        142165708
        0.91%
        0.95%
        0.07%
        0.05%
        129717519
        65265095
        9267858
        3732657
        209.21
        85.00
        0.66%
        0.47%
        0.97
        0.000
        541615412
        0.9406
        75.0%
        89.6%
        95.7%
        267.4M
        47.6%
        558.9M
        56.0%
        98.3%
        71.9%
        tih_rna_sample_00116_B23WHTKLT4_2_1
        127.2bp
        97.9%
        273.6M
        86.9%
        54%
        151bp
        284.2M
        tih_rna_sample_00116_B23WHTKLT4_2_2
        85.9%
        55%
        151bp
        284.2M
        tih_rna_sample_00116_B23WHTKLT4_2_trimmed_1
        87.0%
        55%
        122bp
        279.4M
        87.0%
        55%
        122bp
        279.4M
        tih_rna_sample_00116_B23WHTKLT4_2_trimmed_2
        87.2%
        56%
        122bp
        279.4M
        87.2%
        56%
        122bp
        279.4M
        tih_rna_sample_00121_B23WHTKLT4_1
        144.6bp
        133.0bp
        0.01%
        0.89%
        0.37%
        0.00%
        0.01%
        2.89%
        0.59%
        0.00%
        0.01
        9999000000
        131150109
        No
        0.00%
        591553910
        0.00%
        0.73%
        159934386
        0.91%
        0.95%
        0.07%
        0.05%
        145912476
        73832629
        11121944
        4199371
        270.43
        132.00
        0.71%
        0.55%
        0.97
        0.000
        574255422
        0.9342
        73.3%
        90.5%
        96.1%
        284.3M
        50.1%
        591.6M
        55.7%
        98.6%
        57.1%
        tih_rna_sample_00121_B23WHTKLT4_1_1
        144.6bp
        98.0%
        289.9M
        87.1%
        54%
        151bp
        299.9M
        tih_rna_sample_00121_B23WHTKLT4_1_2
        86.2%
        55%
        151bp
        299.9M
        tih_rna_sample_00121_B23WHTKLT4_1_trimmed_1
        87.1%
        55%
        142bp
        295.8M
        87.1%
        55%
        142bp
        295.8M
        tih_rna_sample_00121_B23WHTKLT4_1_trimmed_2
        87.3%
        55%
        142bp
        295.8M
        87.3%
        55%
        142bp
        295.8M
        tih_rna_sample_00121_B23WHTKLT4_2
        148.6bp
        137.0bp
        0.01%
        0.89%
        0.37%
        0.00%
        0.01%
        2.90%
        0.60%
        0.00%
        0.01
        9999000000
        231961606
        No
        0.00%
        592307678
        0.01%
        0.59%
        242594588
        0.93%
        0.96%
        0.06%
        0.04%
        226081906
        114403844
        13523261
        4809847
        435.41
        225.00
        0.75%
        0.63%
        0.98
        0.000
        575218782
        0.9363
        59.3%
        90.6%
        96.1%
        284.7M
        41.6%
        592.3M
        55.5%
        98.7%
        54.5%
        tih_rna_sample_00121_B23WHTKLT4_2_1
        148.6bp
        98.0%
        290.3M
        84.2%
        54%
        151bp
        303.7M
        tih_rna_sample_00121_B23WHTKLT4_2_2
        83.8%
        55%
        151bp
        303.7M
        tih_rna_sample_00121_B23WHTKLT4_2_trimmed_1
        84.1%
        55%
        142bp
        296.2M
        84.1%
        55%
        142bp
        296.2M
        tih_rna_sample_00121_B23WHTKLT4_2_trimmed_2
        84.7%
        55%
        142bp
        296.2M
        84.7%
        55%
        142bp
        296.2M
        tih_rna_sample_00124_B23WHTKLT4_1
        142.6bp
        130.0bp
        0.01%
        0.98%
        0.42%
        0.00%
        0.01%
        3.17%
        0.68%
        0.00%
        0.01
        9999000000
        123288556
        No
        0.00%
        459190212
        0.01%
        0.69%
        142198926
        0.92%
        0.95%
        0.06%
        0.05%
        130550831
        66052591
        9051535
        3345031
        242.81
        118.00
        0.70%
        0.53%
        0.98
        0.000
        446013317
        0.9400
        69.4%
        90.9%
        96.2%
        220.7M
        48.1%
        459.2M
        54.9%
        98.6%
        58.0%
        tih_rna_sample_00124_B23WHTKLT4_1_1
        142.6bp
        97.9%
        224.8M
        85.4%
        54%
        151bp
        232.8M
        tih_rna_sample_00124_B23WHTKLT4_1_2
        84.4%
        54%
        151bp
        232.8M
        tih_rna_sample_00124_B23WHTKLT4_1_trimmed_1
        85.4%
        54%
        137bp
        229.6M
        85.4%
        54%
        137bp
        229.6M
        tih_rna_sample_00124_B23WHTKLT4_1_trimmed_2
        85.5%
        55%
        137bp
        229.6M
        85.5%
        55%
        137bp
        229.6M
        tih_rna_sample_00124_B23WHTKLT4_2
        150.3bp
        137.0bp
        0.01%
        0.99%
        0.41%
        0.00%
        0.01%
        3.22%
        0.67%
        0.00%
        0.01
        9999000000
        177696333
        No
        0.00%
        450056268
        0.01%
        0.57%
        191520383
        0.93%
        0.96%
        0.05%
        0.04%
        178672028
        90456414
        10216835
        3552072
        345.68
        176.00
        0.73%
        0.60%
        0.98
        0.000
        437643923
        0.9541
        57.8%
        91.2%
        96.2%
        216.5M
        40.4%
        450.1M
        54.3%
        98.8%
        53.6%
        tih_rna_sample_00124_B23WHTKLT4_2_1
        150.3bp
        97.9%
        220.3M
        83.5%
        53%
        151bp
        227.9M
        tih_rna_sample_00124_B23WHTKLT4_2_2
        82.6%
        54%
        151bp
        227.9M
        tih_rna_sample_00124_B23WHTKLT4_2_trimmed_1
        83.6%
        54%
        147bp
        225.0M
        83.6%
        54%
        147bp
        225.0M
        tih_rna_sample_00124_B23WHTKLT4_2_trimmed_2
        83.7%
        54%
        147bp
        225.0M
        83.7%
        54%
        147bp
        225.0M
        tih_rna_sample_00136_B23WHTKLT4_1
        149.2bp
        137.0bp
        0.01%
        1.46%
        0.33%
        0.00%
        0.01%
        4.69%
        0.54%
        0.00%
        0.01
        9999000000
        133355941
        No
        0.00%
        524588758
        0.01%
        0.71%
        153802764
        0.91%
        0.94%
        0.07%
        0.06%
        139999912
        70827883
        11133466
        4301551
        262.53
        130.00
        0.70%
        0.55%
        0.98
        0.000
        507427262
        0.9533
        71.0%
        89.6%
        95.8%
        251.4M
        50.5%
        524.6M
        54.8%
        98.7%
        54.6%
        tih_rna_sample_00136_B23WHTKLT4_1_1
        149.2bp
        97.9%
        256.7M
        85.6%
        53%
        151bp
        265.8M
        tih_rna_sample_00136_B23WHTKLT4_1_2
        84.6%
        54%
        151bp
        265.8M
        tih_rna_sample_00136_B23WHTKLT4_1_trimmed_1
        85.6%
        54%
        142bp
        262.3M
        85.6%
        54%
        142bp
        262.3M
        tih_rna_sample_00136_B23WHTKLT4_1_trimmed_2
        85.7%
        55%
        142bp
        262.3M
        85.7%
        55%
        142bp
        262.3M
        tih_rna_sample_00136_B23WHTKLT4_2
        147.0bp
        135.0bp
        0.01%
        1.47%
        0.33%
        0.00%
        0.01%
        4.72%
        0.55%
        0.00%
        0.02
        9999000000
        180750571
        No
        0.00%
        531926546
        0.01%
        0.60%
        211276327
        0.92%
        0.95%
        0.06%
        0.05%
        195249518
        98714307
        13290358
        5017381
        367.83
        187.00
        0.74%
        0.61%
        0.98
        0.000
        514010506
        0.9506
        60.6%
        89.3%
        95.8%
        254.7M
        40.9%
        531.9M
        55.2%
        98.6%
        56.6%
        tih_rna_sample_00136_B23WHTKLT4_2_1
        147.0bp
        97.9%
        260.4M
        83.2%
        54%
        151bp
        269.7M
        tih_rna_sample_00136_B23WHTKLT4_2_2
        82.3%
        55%
        151bp
        269.7M
        tih_rna_sample_00136_B23WHTKLT4_2_trimmed_1
        83.3%
        54%
        142bp
        266.0M
        83.3%
        54%
        142bp
        266.0M
        tih_rna_sample_00136_B23WHTKLT4_2_trimmed_2
        83.3%
        55%
        142bp
        266.0M
        83.3%
        55%
        142bp
        266.0M
        tih_rna_sample_00145_B23WHTKLT4_1
        137.4bp
        129.0bp
        0.01%
        0.86%
        0.45%
        0.00%
        0.01%
        2.69%
        0.71%
        0.00%
        5.80
        9999000000
        89596863
        No
        0.00%
        591470392
        0.01%
        0.84%
        97456983
        0.88%
        0.93%
        0.08%
        0.07%
        86158364
        43402085
        8058969
        3341525
        144.52
        64.00
        0.62%
        0.40%
        0.97
        0.000
        572667256
        0.9346
        84.0%
        89.3%
        95.6%
        282.7M
        60.4%
        591.5M
        55.8%
        98.6%
        63.2%
        tih_rna_sample_00145_B23WHTKLT4_1_1
        137.4bp
        98.3%
        290.8M
        90.4%
        55%
        151bp
        338.4M
        tih_rna_sample_00145_B23WHTKLT4_1_2
        89.3%
        55%
        151bp
        338.4M
        tih_rna_sample_00145_B23WHTKLT4_1_trimmed_1
        89.8%
        55%
        132bp
        295.7M
        89.8%
        55%
        132bp
        295.7M
        tih_rna_sample_00145_B23WHTKLT4_1_trimmed_2
        89.8%
        55%
        132bp
        295.7M
        89.8%
        55%
        132bp
        295.7M
        tih_rna_sample_00145_B23WHTKLT4_2
        128.0bp
        119.0bp
        0.01%
        0.91%
        0.46%
        0.00%
        0.02%
        2.79%
        0.74%
        0.00%
        5.70
        9999000000
        128675402
        No
        0.00%
        582906764
        0.01%
        0.73%
        158287236
        0.92%
        0.95%
        0.06%
        0.05%
        144932417
        72980006
        10161112
        4104214
        240.12
        112.00
        0.69%
        0.52%
        0.98
        0.000
        562815767
        0.9211
        73.2%
        88.6%
        95.4%
        278.1M
        46.4%
        582.9M
        56.2%
        98.5%
        70.3%
        tih_rna_sample_00145_B23WHTKLT4_2_1
        128.0bp
        98.3%
        286.6M
        86.7%
        54%
        151bp
        295.8M
        tih_rna_sample_00145_B23WHTKLT4_2_2
        85.9%
        55%
        151bp
        295.8M
        tih_rna_sample_00145_B23WHTKLT4_2_trimmed_1
        86.8%
        55%
        122bp
        291.5M
        86.8%
        55%
        122bp
        291.5M
        tih_rna_sample_00145_B23WHTKLT4_2_trimmed_2
        87.1%
        56%
        122bp
        291.5M
        87.1%
        56%
        122bp
        291.5M
        tih_rna_sample_00146_B23WHTKLT4_1
        166.3bp
        151.0bp
        0.00%
        0.53%
        0.39%
        0.00%
        0.01%
        1.76%
        0.62%
        0.00%
        0.01
        9999000000
        165534778
        No
        0.00%
        591862464
        0.01%
        0.66%
        199634375
        0.92%
        0.96%
        0.06%
        0.04%
        184307072
        93690748
        12212629
        3933481
        378.27
        186.00
        0.73%
        0.61%
        0.97
        0.000
        576390137
        0.9470
        66.6%
        91.6%
        96.5%
        285.5M
        47.6%
        591.9M
        55.7%
        98.6%
        43.5%
        tih_rna_sample_00146_B23WHTKLT4_1_1
        166.3bp
        97.8%
        289.3M
        86.9%
        55%
        151bp
        325.3M
        tih_rna_sample_00146_B23WHTKLT4_1_2
        86.3%
        55%
        151bp
        325.3M
        tih_rna_sample_00146_B23WHTKLT4_1_trimmed_1
        86.4%
        55%
        151bp
        295.9M
        86.4%
        55%
        151bp
        295.9M
        tih_rna_sample_00146_B23WHTKLT4_1_trimmed_2
        86.8%
        56%
        151bp
        295.9M
        86.8%
        56%
        151bp
        295.9M
        tih_rna_sample_00146_B23WHTKLT4_2
        161.1bp
        145.0bp
        0.01%
        0.56%
        0.39%
        0.00%
        0.01%
        1.83%
        0.62%
        0.00%
        0.01
        9999000000
        243097117
        No
        0.00%
        592175412
        0.01%
        0.55%
        263912483
        0.93%
        0.97%
        0.05%
        0.03%
        246494054
        125209941
        14330211
        4533056
        505.15
        257.00
        0.76%
        0.65%
        0.97
        0.000
        576358972
        0.9526
        55.7%
        91.4%
        96.4%
        285.6M
        39.6%
        592.2M
        55.6%
        98.7%
        46.9%
        tih_rna_sample_00146_B23WHTKLT4_2_1
        161.1bp
        97.8%
        289.4M
        86.8%
        54%
        151bp
        399.3M
        tih_rna_sample_00146_B23WHTKLT4_2_2
        86.1%
        55%
        151bp
        399.3M
        tih_rna_sample_00146_B23WHTKLT4_2_trimmed_1
        84.8%
        55%
        151bp
        296.1M
        84.8%
        55%
        151bp
        296.1M
        tih_rna_sample_00146_B23WHTKLT4_2_trimmed_2
        85.2%
        55%
        151bp
        296.1M
        85.2%
        55%
        151bp
        296.1M
        tih_rna_sample_00149_B23WHTKLT4_1
        138.7bp
        128.0bp
        0.01%
        0.94%
        0.35%
        0.00%
        0.02%
        2.91%
        0.57%
        0.00%
        0.01
        9999000000
        102945208
        No
        0.00%
        465710252
        0.00%
        0.73%
        123867653
        0.91%
        0.94%
        0.07%
        0.06%
        112544518
        56807109
        9201875
        3699496
        196.20
        87.00
        0.65%
        0.47%
        0.98
        0.000
        452090199
        0.9436
        73.7%
        90.2%
        96.0%
        223.5M
        50.3%
        465.7M
        54.0%
        98.6%
        62.8%
        tih_rna_sample_00149_B23WHTKLT4_1_1
        138.7bp
        98.0%
        228.1M
        85.9%
        53%
        151bp
        236.2M
        tih_rna_sample_00149_B23WHTKLT4_1_2
        84.9%
        54%
        151bp
        236.2M
        tih_rna_sample_00149_B23WHTKLT4_1_trimmed_1
        86.0%
        53%
        132bp
        232.9M
        86.0%
        53%
        132bp
        232.9M
        tih_rna_sample_00149_B23WHTKLT4_1_trimmed_2
        85.9%
        54%
        132bp
        232.9M
        85.9%
        54%
        132bp
        232.9M
        tih_rna_sample_00149_B23WHTKLT4_2
        139.2bp
        129.0bp
        0.01%
        0.95%
        0.36%
        0.00%
        0.02%
        2.93%
        0.57%
        0.00%
        0.03
        9999000000
        165641871
        No
        0.00%
        502762086
        0.00%
        0.63%
        184386317
        0.92%
        0.95%
        0.06%
        0.05%
        170488283
        86064025
        11630717
        4540918
        303.58
        140.00
        0.70%
        0.56%
        0.98
        0.000
        488041347
        0.9407
        63.6%
        90.1%
        96.0%
        241.3M
        43.4%
        502.8M
        54.0%
        98.7%
        62.4%
        tih_rna_sample_00149_B23WHTKLT4_2_1
        139.2bp
        97.9%
        246.1M
        84.0%
        53%
        151bp
        254.7M
        tih_rna_sample_00149_B23WHTKLT4_2_2
        83.2%
        54%
        151bp
        254.7M
        tih_rna_sample_00149_B23WHTKLT4_2_trimmed_1
        84.0%
        53%
        132bp
        251.4M
        84.0%
        53%
        132bp
        251.4M
        tih_rna_sample_00149_B23WHTKLT4_2_trimmed_2
        84.2%
        54%
        132bp
        251.4M
        84.2%
        54%
        132bp
        251.4M
        tih_rna_sample_00150_B23WHTKLT4_1
        146.4bp
        133.0bp
        0.01%
        1.24%
        0.54%
        0.00%
        0.02%
        3.45%
        0.75%
        0.00%
        0.02
        9999000000
        143952144
        No
        0.00%
        495361284
        0.00%
        0.65%
        173073795
        0.93%
        0.96%
        0.06%
        0.04%
        161035429
        81462970
        9873565
        3464532
        300.38
        116.00
        0.67%
        0.53%
        0.98
        0.000
        482657608
        0.9700
        65.3%
        91.4%
        96.5%
        238.9M
        44.7%
        495.4M
        54.1%
        98.5%
        57.2%
        tih_rna_sample_00150_B23WHTKLT4_1_1
        146.4bp
        98.2%
        243.3M
        85.7%
        53%
        151bp
        251.5M
        tih_rna_sample_00150_B23WHTKLT4_1_2
        85.0%
        54%
        151bp
        251.5M
        tih_rna_sample_00150_B23WHTKLT4_1_trimmed_1
        85.8%
        53%
        137bp
        247.7M
        85.8%
        53%
        137bp
        247.7M
        tih_rna_sample_00150_B23WHTKLT4_1_trimmed_2
        86.2%
        54%
        142bp
        247.7M
        86.2%
        54%
        142bp
        247.7M
        tih_rna_sample_00150_B23WHTKLT4_2
        147.2bp
        134.0bp
        0.01%
        1.25%
        0.54%
        0.00%
        0.03%
        3.47%
        0.75%
        0.00%
        0.00
        9999000000
        258166992
        No
        0.00%
        591983976
        0.00%
        0.55%
        266371190
        0.94%
        0.97%
        0.05%
        0.03%
        250276778
        126698933
        13422262
        4511887
        481.71
        194.00
        0.72%
        0.60%
        0.97
        0.000
        576778684
        0.9650
        55.3%
        91.3%
        96.5%
        285.5M
        37.5%
        592.0M
        54.5%
        98.7%
        56.5%
        tih_rna_sample_00150_B23WHTKLT4_2_1
        147.2bp
        98.2%
        290.7M
        86.7%
        53%
        151bp
        390.3M
        tih_rna_sample_00150_B23WHTKLT4_2_2
        86.1%
        54%
        151bp
        390.3M
        tih_rna_sample_00150_B23WHTKLT4_2_trimmed_1
        84.9%
        54%
        142bp
        296.0M
        84.9%
        54%
        142bp
        296.0M
        tih_rna_sample_00150_B23WHTKLT4_2_trimmed_2
        85.3%
        54%
        142bp
        296.0M
        85.3%
        54%
        142bp
        296.0M
        tih_rna_sample_00158_B23WHTKLT4_1
        154.1bp
        140.0bp
        0.02%
        1.41%
        0.52%
        0.00%
        0.04%
        4.10%
        0.78%
        0.00%
        5.08
        9999000000
        73716861
        No
        0.00%
        442961900
        0.01%
        0.83%
        76831363
        0.87%
        0.93%
        0.09%
        0.07%
        67124514
        34047158
        7145995
        2730134
        127.53
        48.00
        0.58%
        0.34%
        0.97
        0.000
        424959289
        0.9553
        83.1%
        87.5%
        95.1%
        210.6M
        61.4%
        443.0M
        58.1%
        98.5%
        51.0%
        tih_rna_sample_00158_B23WHTKLT4_1_1
        154.1bp
        97.7%
        216.4M
        89.3%
        57%
        151bp
        224.8M
        tih_rna_sample_00158_B23WHTKLT4_1_2
        87.9%
        57%
        151bp
        224.8M
        tih_rna_sample_00158_B23WHTKLT4_1_trimmed_1
        89.4%
        57%
        147bp
        221.5M
        89.4%
        57%
        147bp
        221.5M
        tih_rna_sample_00158_B23WHTKLT4_1_trimmed_2
        89.1%
        58%
        147bp
        221.5M
        89.1%
        58%
        147bp
        221.5M
        tih_rna_sample_00158_B23WHTKLT4_2
        149.7bp
        135.0bp
        0.03%
        1.45%
        0.53%
        0.00%
        0.05%
        4.19%
        0.78%
        0.00%
        5.12
        9999000000
        79587493
        No
        0.00%
        319843414
        0.01%
        0.69%
        97824024
        0.91%
        0.95%
        0.07%
        0.05%
        89128358
        45125099
        6842053
        2468066
        167.97
        66.00
        0.62%
        0.42%
        0.97
        0.000
        306358495
        0.9630
        69.8%
        87.1%
        95.0%
        151.9M
        47.8%
        319.8M
        57.9%
        98.1%
        53.7%
        tih_rna_sample_00158_B23WHTKLT4_2_1
        149.7bp
        97.8%
        156.3M
        84.5%
        56%
        151bp
        163.0M
        tih_rna_sample_00158_B23WHTKLT4_2_2
        83.4%
        57%
        151bp
        163.0M
        tih_rna_sample_00158_B23WHTKLT4_2_trimmed_1
        84.5%
        57%
        142bp
        159.9M
        84.5%
        57%
        142bp
        159.9M
        tih_rna_sample_00158_B23WHTKLT4_2_trimmed_2
        84.7%
        58%
        142bp
        159.9M
        84.7%
        58%
        142bp
        159.9M
        tih_rna_sample_00161_B23WHTKLT4_1
        136.6bp
        126.0bp
        0.01%
        0.88%
        0.45%
        0.00%
        0.01%
        2.83%
        0.72%
        0.00%
        0.02
        9999000000
        112938154
        No
        0.00%
        579572052
        0.00%
        0.79%
        119436960
        0.90%
        0.94%
        0.08%
        0.06%
        107683540
        54346697
        9028250
        3598626
        186.34
        79.00
        0.65%
        0.45%
        0.97
        0.000
        561902103
        0.9251
        79.8%
        89.4%
        96.0%
        278.1M
        54.8%
        579.6M
        56.0%
        98.6%
        63.6%
        tih_rna_sample_00161_B23WHTKLT4_1_1
        136.6bp
        98.3%
        284.8M
        88.4%
        55%
        151bp
        293.8M
        tih_rna_sample_00161_B23WHTKLT4_1_2
        87.4%
        55%
        151bp
        293.8M
        tih_rna_sample_00161_B23WHTKLT4_1_trimmed_1
        88.5%
        55%
        132bp
        289.8M
        88.5%
        55%
        132bp
        289.8M
        tih_rna_sample_00161_B23WHTKLT4_1_trimmed_2
        88.5%
        56%
        132bp
        289.8M
        88.5%
        56%
        132bp
        289.8M
        tih_rna_sample_00161_B23WHTKLT4_2
        141.2bp
        132.0bp
        0.01%
        0.88%
        0.45%
        0.00%
        0.01%
        2.83%
        0.71%
        0.00%
        0.02
        9999000000
        135062595
        No
        0.00%
        591612000
        0.00%
        0.71%
        171123468
        0.92%
        0.95%
        0.06%
        0.05%
        157312070
        79490145
        10997280
        4164964
        285.92
        126.00
        0.70%
        0.54%
        0.97
        0.000
        573785219
        0.9298
        71.4%
        89.4%
        96.0%
        283.9M
        49.8%
        591.6M
        55.9%
        98.6%
        60.2%
        tih_rna_sample_00161_B23WHTKLT4_2_1
        141.2bp
        98.2%
        290.5M
        88.2%
        55%
        151bp
        377.6M
        tih_rna_sample_00161_B23WHTKLT4_2_2
        87.3%
        55%
        151bp
        377.6M
        tih_rna_sample_00161_B23WHTKLT4_2_trimmed_1
        86.6%
        55%
        137bp
        295.8M
        86.6%
        55%
        137bp
        295.8M
        tih_rna_sample_00161_B23WHTKLT4_2_trimmed_2
        86.7%
        56%
        137bp
        295.8M
        86.7%
        56%
        137bp
        295.8M
        tih_rna_sample_00163_B23WHTKLT4_1
        153.4bp
        140.0bp
        0.01%
        1.30%
        0.41%
        0.00%
        0.02%
        4.09%
        0.66%
        0.00%
        0.00
        9999000000
        127339028
        No
        0.00%
        372716578
        0.00%
        0.60%
        148441779
        0.93%
        0.96%
        0.06%
        0.04%
        138395026
        70112147
        8365815
        2885002
        270.39
        123.00
        0.69%
        0.54%
        0.98
        0.000
        361905686
        0.9409
        60.4%
        90.5%
        96.2%
        179.3M
        42.7%
        372.7M
        54.8%
        98.6%
        51.4%
        tih_rna_sample_00163_B23WHTKLT4_1_1
        153.4bp
        98.1%
        182.9M
        82.6%
        54%
        151bp
        188.9M
        tih_rna_sample_00163_B23WHTKLT4_1_2
        81.9%
        54%
        151bp
        188.9M
        tih_rna_sample_00163_B23WHTKLT4_1_trimmed_1
        82.7%
        54%
        147bp
        186.4M
        82.7%
        54%
        147bp
        186.4M
        tih_rna_sample_00163_B23WHTKLT4_1_trimmed_2
        83.0%
        55%
        147bp
        186.4M
        83.0%
        55%
        147bp
        186.4M
        tih_rna_sample_00163_B23WHTKLT4_2
        148.3bp
        134.0bp
        0.01%
        1.36%
        0.42%
        0.00%
        0.02%
        4.25%
        0.68%
        0.00%
        0.01
        9999000000
        191617122
        No
        0.00%
        590349796
        0.00%
        0.62%
        222090069
        0.93%
        0.96%
        0.06%
        0.04%
        206321140
        104460096
        12965061
        4517031
        395.43
        187.00
        0.73%
        0.60%
        0.98
        0.000
        572341097
        0.9390
        62.7%
        90.2%
        96.1%
        283.6M
        42.6%
        590.3M
        54.7%
        98.4%
        54.5%
        tih_rna_sample_00163_B23WHTKLT4_2_1
        148.3bp
        98.1%
        289.7M
        85.8%
        54%
        151bp
        324.4M
        tih_rna_sample_00163_B23WHTKLT4_2_2
        85.2%
        54%
        151bp
        324.4M
        tih_rna_sample_00163_B23WHTKLT4_2_trimmed_1
        85.3%
        54%
        142bp
        295.2M
        85.3%
        54%
        142bp
        295.2M
        tih_rna_sample_00163_B23WHTKLT4_2_trimmed_2
        85.9%
        54%
        142bp
        295.2M
        85.9%
        54%
        142bp
        295.2M
        tih_rna_sample_00164_B23WHTKLT4_1
        135.8bp
        126.0bp
        0.01%
        0.83%
        0.43%
        0.00%
        0.02%
        2.49%
        0.65%
        0.00%
        0.00
        9999000000
        53748044
        No
        0.00%
        494548986
        0.01%
        0.88%
        57272375
        0.84%
        0.91%
        0.10%
        0.09%
        48030543
        24210162
        5798197
        2409554
        80.11
        28.00
        0.49%
        0.21%
        0.97
        0.000
        476807983
        0.9322
        89.0%
        87.8%
        95.4%
        235.9M
        62.9%
        494.5M
        56.8%
        98.4%
        65.0%
        tih_rna_sample_00164_B23WHTKLT4_1_1
        135.8bp
        98.2%
        242.8M
        91.4%
        55%
        151bp
        251.4M
        tih_rna_sample_00164_B23WHTKLT4_1_2
        89.8%
        56%
        151bp
        251.4M
        tih_rna_sample_00164_B23WHTKLT4_1_trimmed_1
        91.5%
        56%
        132bp
        247.3M
        91.5%
        56%
        132bp
        247.3M
        tih_rna_sample_00164_B23WHTKLT4_1_trimmed_2
        91.0%
        57%
        132bp
        247.3M
        91.0%
        57%
        132bp
        247.3M
        tih_rna_sample_00164_B23WHTKLT4_2
        133.2bp
        123.0bp
        0.01%
        0.80%
        0.40%
        0.00%
        0.02%
        2.38%
        0.61%
        0.00%
        0.00
        9999000000
        52473351
        No
        0.00%
        268537882
        0.01%
        0.79%
        55370963
        0.88%
        0.93%
        0.08%
        0.07%
        48708093
        24574793
        4542539
        1824034
        81.93
        28.00
        0.49%
        0.22%
        0.97
        0.000
        257935430
        0.9519
        80.0%
        85.5%
        94.9%
        127.4M
        52.7%
        268.5M
        57.1%
        98.4%
        66.4%
        tih_rna_sample_00164_B23WHTKLT4_2_1
        133.2bp
        97.9%
        131.4M
        86.3%
        55%
        151bp
        136.5M
        tih_rna_sample_00164_B23WHTKLT4_2_2
        84.9%
        56%
        151bp
        136.5M
        tih_rna_sample_00164_B23WHTKLT4_2_trimmed_1
        86.4%
        56%
        127bp
        134.3M
        86.4%
        56%
        127bp
        134.3M
        tih_rna_sample_00164_B23WHTKLT4_2_trimmed_2
        86.1%
        57%
        127bp
        134.3M
        86.1%
        57%
        127bp
        134.3M
        tih_rna_sample_00166_B23WHTKLT4_1
        147.9bp
        135.0bp
        0.01%
        0.97%
        0.41%
        0.00%
        0.01%
        3.13%
        0.67%
        0.00%
        6.21
        9999000000
        113652557
        No
        0.00%
        590697846
        0.00%
        0.77%
        134707465
        0.91%
        0.95%
        0.07%
        0.05%
        123110137
        62216241
        8876247
        3262548
        228.37
        104.00
        0.67%
        0.51%
        0.98
        0.000
        573056989
        0.9345
        77.6%
        90.6%
        96.1%
        283.7M
        55.9%
        590.7M
        54.0%
        98.4%
        55.1%
        tih_rna_sample_00166_B23WHTKLT4_1_1
        147.9bp
        98.4%
        290.7M
        88.9%
        53%
        151bp
        312.5M
        tih_rna_sample_00166_B23WHTKLT4_1_2
        87.8%
        53%
        151bp
        312.5M
        tih_rna_sample_00166_B23WHTKLT4_1_trimmed_1
        88.7%
        53%
        142bp
        295.3M
        88.7%
        53%
        142bp
        295.3M
        tih_rna_sample_00166_B23WHTKLT4_1_trimmed_2
        88.8%
        54%
        142bp
        295.3M
        88.8%
        54%
        142bp
        295.3M
        tih_rna_sample_00166_B23WHTKLT4_2
        145.1bp
        133.0bp
        0.01%
        0.97%
        0.41%
        0.00%
        0.02%
        3.12%
        0.67%
        0.00%
        6.08
        9999000000
        163730854
        No
        0.00%
        458492152
        0.00%
        0.59%
        189488002
        0.94%
        0.97%
        0.05%
        0.03%
        178109889
        90036950
        9280270
        3198719
        339.90
        163.00
        0.72%
        0.58%
        0.98
        0.000
        444718653
        0.9473
        58.9%
        90.5%
        96.1%
        220.2M
        40.1%
        458.5M
        54.6%
        98.8%
        56.6%
        tih_rna_sample_00166_B23WHTKLT4_2_1
        145.1bp
        98.4%
        225.7M
        83.9%
        54%
        151bp
        232.1M
        tih_rna_sample_00166_B23WHTKLT4_2_2
        83.3%
        54%
        151bp
        232.1M
        tih_rna_sample_00166_B23WHTKLT4_2_trimmed_1
        83.9%
        54%
        142bp
        229.2M
        83.9%
        54%
        142bp
        229.2M
        tih_rna_sample_00166_B23WHTKLT4_2_trimmed_2
        84.3%
        54%
        142bp
        229.2M
        84.3%
        54%
        142bp
        229.2M
        tih_rna_sample_00434_B23WHTKLT4_1
        134.6bp
        124.0bp
        0.01%
        1.17%
        0.50%
        0.00%
        0.01%
        3.46%
        0.75%
        0.00%
        0.01
        9999000000
        114181195
        No
        0.00%
        591290026
        0.00%
        0.77%
        137754147
        0.91%
        0.94%
        0.07%
        0.06%
        125629420
        63367572
        9533061
        3740341
        223.08
        109.00
        0.69%
        0.52%
        0.97
        0.000
        571809131
        0.8994
        77.0%
        89.6%
        95.7%
        283.0M
        50.5%
        591.3M
        55.6%
        98.5%
        64.1%
        tih_rna_sample_00434_B23WHTKLT4_1_1
        134.6bp
        98.4%
        291.0M
        90.0%
        54%
        151bp
        420.8M
        tih_rna_sample_00434_B23WHTKLT4_1_2
        88.9%
        55%
        151bp
        420.8M
        tih_rna_sample_00434_B23WHTKLT4_1_trimmed_1
        87.9%
        55%
        132bp
        295.6M
        87.9%
        55%
        132bp
        295.6M
        tih_rna_sample_00434_B23WHTKLT4_1_trimmed_2
        88.0%
        55%
        132bp
        295.6M
        88.0%
        55%
        132bp
        295.6M
        tih_rna_sample_00434_B23WHTKLT4_2
        143.3bp
        132.0bp
        0.01%
        1.14%
        0.48%
        0.00%
        0.02%
        3.44%
        0.72%
        0.00%
        0.01
        9999000000
        176853175
        No
        0.00%
        591783656
        0.00%
        0.64%
        213727639
        0.93%
        0.96%
        0.06%
        0.04%
        199366774
        100757455
        11769349
        4274588
        371.65
        193.00
        0.74%
        0.61%
        0.97
        0.000
        573001391
        0.9269
        64.2%
        89.9%
        95.8%
        283.5M
        43.7%
        591.8M
        54.6%
        98.6%
        58.7%
        tih_rna_sample_00434_B23WHTKLT4_2_1
        143.3bp
        98.4%
        291.1M
        87.9%
        53%
        151bp
        412.3M
        tih_rna_sample_00434_B23WHTKLT4_2_2
        87.1%
        54%
        151bp
        412.3M
        tih_rna_sample_00434_B23WHTKLT4_2_trimmed_1
        85.6%
        54%
        137bp
        295.9M
        85.6%
        54%
        137bp
        295.9M
        tih_rna_sample_00434_B23WHTKLT4_2_trimmed_2
        85.9%
        54%
        137bp
        295.9M
        85.9%
        54%
        137bp
        295.9M
        tih_rna_sample_00451_B23WHTKLT4_1
        138.5bp
        128.0bp
        0.01%
        0.69%
        0.41%
        0.00%
        0.01%
        2.16%
        0.61%
        0.00%
        5.96
        9999000000
        157481381
        No
        0.00%
        573379982
        0.01%
        0.67%
        189721141
        0.92%
        0.95%
        0.07%
        0.05%
        174226945
        87946530
        12382225
        4862106
        308.11
        179.00
        0.76%
        0.62%
        0.98
        0.000
        554704143
        0.9249
        67.3%
        89.3%
        95.7%
        274.2M
        45.6%
        573.4M
        53.9%
        98.7%
        62.5%
        tih_rna_sample_00451_B23WHTKLT4_1_1
        138.5bp
        98.0%
        280.9M
        84.9%
        53%
        151bp
        290.5M
        tih_rna_sample_00451_B23WHTKLT4_1_2
        84.3%
        54%
        151bp
        290.5M
        tih_rna_sample_00451_B23WHTKLT4_1_trimmed_1
        85.0%
        53%
        132bp
        286.7M
        85.0%
        53%
        132bp
        286.7M
        tih_rna_sample_00451_B23WHTKLT4_1_trimmed_2
        85.4%
        54%
        132bp
        286.7M
        85.4%
        54%
        132bp
        286.7M
        tih_rna_sample_00451_B23WHTKLT4_2
        136.0bp
        126.0bp
        0.01%
        0.70%
        0.42%
        0.00%
        0.01%
        2.19%
        0.61%
        0.00%
        5.93
        9999000000
        229535546
        No
        0.00%
        345125830
        0.01%
        0.43%
        195072298
        0.94%
        0.96%
        0.05%
        0.04%
        183279181
        92453109
        9838543
        3708700
        330.06
        193.00
        0.77%
        0.63%
        0.98
        0.000
        333519732
        0.9308
        43.7%
        89.1%
        95.5%
        164.9M
        27.5%
        345.1M
        54.1%
        98.7%
        63.7%
        tih_rna_sample_00451_B23WHTKLT4_2_1
        136.0bp
        98.0%
        169.1M
        77.4%
        53%
        151bp
        174.9M
        tih_rna_sample_00451_B23WHTKLT4_2_2
        77.0%
        53%
        151bp
        174.9M
        tih_rna_sample_00451_B23WHTKLT4_2_trimmed_1
        77.4%
        53%
        132bp
        172.6M
        77.4%
        53%
        132bp
        172.6M
        tih_rna_sample_00451_B23WHTKLT4_2_trimmed_2
        78.0%
        54%
        132bp
        172.6M
        78.0%
        54%
        132bp
        172.6M
        tih_rna_sample_00457_B23WHTKLT4_1
        151.8bp
        138.0bp
        0.01%
        0.68%
        0.36%
        0.00%
        0.01%
        2.23%
        0.57%
        0.00%
        4.68
        9999000000
        142488876
        No
        0.00%
        591255714
        0.00%
        0.71%
        170126561
        0.93%
        0.96%
        0.06%
        0.04%
        157501652
        79720036
        9731684
        3340506
        304.08
        132.00
        0.71%
        0.55%
        0.97
        0.000
        575511529
        0.9571
        71.6%
        91.2%
        96.4%
        284.9M
        50.6%
        591.3M
        55.7%
        98.5%
        53.3%
        tih_rna_sample_00457_B23WHTKLT4_1_1
        151.8bp
        98.4%
        291.0M
        89.8%
        55%
        151bp
        405.7M
        tih_rna_sample_00457_B23WHTKLT4_1_2
        88.8%
        55%
        151bp
        405.7M
        tih_rna_sample_00457_B23WHTKLT4_1_trimmed_1
        87.9%
        55%
        147bp
        295.6M
        87.9%
        55%
        147bp
        295.6M
        tih_rna_sample_00457_B23WHTKLT4_1_trimmed_2
        88.1%
        55%
        147bp
        295.6M
        88.1%
        55%
        147bp
        295.6M
        tih_rna_sample_00457_B23WHTKLT4_2
        150.1bp
        137.0bp
        0.01%
        0.68%
        0.37%
        0.00%
        0.01%
        2.21%
        0.58%
        0.00%
        4.58
        9999000000
        169616747
        No
        0.00%
        591890736
        0.01%
        0.65%
        206836235
        0.93%
        0.96%
        0.05%
        0.04%
        193182086
        97770202
        10681356
        3574164
        375.49
        164.00
        0.73%
        0.59%
        0.97
        0.000
        575833878
        0.9552
        65.4%
        91.0%
        96.3%
        285.0M
        46.1%
        591.9M
        56.2%
        98.6%
        54.1%
        tih_rna_sample_00457_B23WHTKLT4_2_1
        150.1bp
        98.4%
        291.3M
        86.8%
        55%
        151bp
        306.4M
        tih_rna_sample_00457_B23WHTKLT4_2_2
        86.0%
        56%
        151bp
        306.4M
        tih_rna_sample_00457_B23WHTKLT4_2_trimmed_1
        86.8%
        55%
        142bp
        295.9M
        86.8%
        55%
        142bp
        295.9M
        tih_rna_sample_00457_B23WHTKLT4_2_trimmed_2
        86.9%
        56%
        142bp
        295.9M
        86.9%
        56%
        142bp
        295.9M
        tih_rna_sample_00461_B23WHTKLT4_1
        153.6bp
        140.0bp
        0.01%
        0.96%
        0.48%
        0.00%
        0.02%
        2.84%
        0.69%
        0.00%
        2.72
        9999000000
        9450203
        No
        0.00%
        101224170
        0.00%
        0.92%
        8254201
        0.82%
        0.89%
        0.13%
        0.11%
        6764310
        3421303
        1057314
        426391
        12.41
        4.00
        0.10%
        0.01%
        0.97
        0.000
        98043098
        0.9723
        92.2%
        89.5%
        96.0%
        48.6M
        69.2%
        101.2M
        54.6%
        97.3%
        52.3%
        tih_rna_sample_00461_B23WHTKLT4_1_1
        153.6bp
        98.2%
        49.7M
        91.8%
        54%
        151bp
        52.0M
        tih_rna_sample_00461_B23WHTKLT4_1_2
        89.8%
        55%
        151bp
        52.0M
        tih_rna_sample_00461_B23WHTKLT4_1_trimmed_1
        91.8%
        54%
        147bp
        50.6M
        91.8%
        54%
        147bp
        50.6M
        tih_rna_sample_00461_B23WHTKLT4_1_trimmed_2
        91.0%
        54%
        147bp
        50.6M
        91.0%
        54%
        147bp
        50.6M
        tih_rna_sample_00461_B23WHTKLT4_2
        147.2bp
        134.0bp
        0.02%
        1.06%
        0.49%
        0.00%
        0.04%
        3.12%
        0.71%
        0.00%
        2.62
        9999000000
        218933094
        No
        0.00%
        384330140
        0.00%
        0.47%
        202736080
        0.94%
        0.97%
        0.05%
        0.03%
        190735049
        96452032
        10282139
        3473760
        365.76
        149.00
        0.68%
        0.55%
        0.97
        0.000
        371527007
        0.9276
        47.5%
        89.0%
        95.8%
        184.0M
        31.4%
        384.3M
        54.2%
        98.3%
        56.1%
        tih_rna_sample_00461_B23WHTKLT4_2_1
        147.2bp
        98.2%
        188.6M
        81.4%
        53%
        151bp
        195.6M
        tih_rna_sample_00461_B23WHTKLT4_2_2
        81.1%
        54%
        151bp
        195.6M
        tih_rna_sample_00461_B23WHTKLT4_2_trimmed_1
        81.4%
        53%
        142bp
        192.2M
        81.4%
        53%
        142bp
        192.2M
        tih_rna_sample_00461_B23WHTKLT4_2_trimmed_2
        82.4%
        54%
        142bp
        192.2M
        82.4%
        54%
        142bp
        192.2M
        tih_rna_sample_00462_B23WHTKLT4_1
        153.0bp
        139.0bp
        0.00%
        0.77%
        0.65%
        0.00%
        0.01%
        2.42%
        0.90%
        0.00%
        6.36
        9999000000
        150819486
        No
        0.00%
        591399094
        0.00%
        0.68%
        187477871
        0.93%
        0.97%
        0.05%
        0.03%
        175157451
        88693221
        9743940
        3189897
        342.74
        135.00
        0.67%
        0.55%
        0.98
        0.000
        577528420
        0.9725
        68.6%
        92.3%
        96.7%
        286.1M
        49.3%
        591.4M
        54.7%
        98.6%
        51.8%
        tih_rna_sample_00462_B23WHTKLT4_1_1
        153.0bp
        98.4%
        290.9M
        89.9%
        54%
        151bp
        431.1M
        tih_rna_sample_00462_B23WHTKLT4_1_2
        89.0%
        54%
        151bp
        431.1M
        tih_rna_sample_00462_B23WHTKLT4_1_trimmed_1
        87.6%
        54%
        147bp
        295.7M
        87.6%
        54%
        147bp
        295.7M
        tih_rna_sample_00462_B23WHTKLT4_1_trimmed_2
        88.0%
        54%
        147bp
        295.7M
        88.0%
        54%
        147bp
        295.7M
        tih_rna_sample_00462_B23WHTKLT4_2
        157.2bp
        143.0bp
        0.00%
        0.75%
        0.65%
        0.00%
        0.01%
        2.37%
        0.90%
        0.00%
        6.31
        9999000000
        249927144
        No
        0.00%
        592168176
        0.00%
        0.55%
        264553359
        0.95%
        0.97%
        0.04%
        0.03%
        250026212
        126639063
        11902731
        3663244
        509.65
        211.00
        0.70%
        0.60%
        0.97
        0.000
        578561710
        0.9717
        55.6%
        92.4%
        96.8%
        286.5M
        39.8%
        592.2M
        54.8%
        98.7%
        49.0%
        tih_rna_sample_00462_B23WHTKLT4_2_1
        157.2bp
        98.3%
        291.1M
        86.0%
        54%
        151bp
        320.2M
        tih_rna_sample_00462_B23WHTKLT4_2_2
        85.4%
        54%
        151bp
        320.2M
        tih_rna_sample_00462_B23WHTKLT4_2_trimmed_1
        85.6%
        54%
        151bp
        296.1M
        85.6%
        54%
        151bp
        296.1M
        tih_rna_sample_00462_B23WHTKLT4_2_trimmed_2
        86.0%
        55%
        151bp
        296.1M
        86.0%
        55%
        151bp
        296.1M
        tih_rna_sample_00464_B23WHTKLT4_1
        139.0bp
        128.0bp
        0.01%
        1.29%
        0.55%
        0.00%
        0.01%
        3.90%
        0.82%
        0.00%
        0.02
        9999000000
        119695884
        No
        0.00%
        548980454
        0.01%
        0.77%
        128760413
        0.90%
        0.94%
        0.07%
        0.06%
        116356559
        58749292
        9453563
        3747221
        204.61
        94.00
        0.67%
        0.49%
        0.97
        0.000
        532022373
        0.9496
        77.0%
        90.1%
        95.9%
        263.2M
        52.3%
        549.0M
        54.0%
        98.6%
        62.1%
        tih_rna_sample_00464_B23WHTKLT4_1_1
        139.0bp
        98.2%
        269.5M
        87.7%
        53%
        151bp
        278.4M
        tih_rna_sample_00464_B23WHTKLT4_1_2
        86.7%
        53%
        151bp
        278.4M
        tih_rna_sample_00464_B23WHTKLT4_1_trimmed_1
        87.8%
        53%
        132bp
        274.5M
        87.8%
        53%
        132bp
        274.5M
        tih_rna_sample_00464_B23WHTKLT4_1_trimmed_2
        87.9%
        54%
        132bp
        274.5M
        87.9%
        54%
        132bp
        274.5M
        tih_rna_sample_00464_B23WHTKLT4_2
        131.2bp
        121.0bp
        0.01%
        1.35%
        0.57%
        0.00%
        0.02%
        4.03%
        0.86%
        0.00%
        0.01
        9999000000
        117613500
        No
        0.00%
        402957022
        0.01%
        0.64%
        146204520
        0.92%
        0.95%
        0.06%
        0.05%
        135236780
        68178642
        8788333
        3404484
        234.35
        111.00
        0.68%
        0.52%
        0.98
        0.000
        389582624
        0.9494
        64.1%
        89.4%
        95.7%
        192.8M
        40.1%
        403.0M
        54.1%
        98.3%
        66.3%
        tih_rna_sample_00464_B23WHTKLT4_2_1
        131.2bp
        98.2%
        197.8M
        83.2%
        53%
        151bp
        204.9M
        tih_rna_sample_00464_B23WHTKLT4_2_2
        82.4%
        54%
        151bp
        204.9M
        tih_rna_sample_00464_B23WHTKLT4_2_trimmed_1
        83.2%
        53%
        127bp
        201.5M
        83.2%
        53%
        127bp
        201.5M
        tih_rna_sample_00464_B23WHTKLT4_2_trimmed_2
        83.7%
        54%
        127bp
        201.5M
        83.7%
        54%
        127bp
        201.5M
        tih_rna_sample_00467_B23WHTKLT4_1
        130.5bp
        123.0bp
        0.01%
        1.34%
        0.49%
        0.00%
        0.01%
        4.03%
        0.79%
        0.00%
        0.02
        9999000000
        98862201
        No
        0.00%
        578835000
        0.01%
        0.82%
        101713660
        0.86%
        0.91%
        0.10%
        0.09%
        87674160
        44121632
        10072620
        4353314
        140.84
        53.00
        0.60%
        0.36%
        0.97
        0.000
        553019223
        0.9432
        83.0%
        85.1%
        94.5%
        273.6M
        55.7%
        578.8M
        58.4%
        98.4%
        69.8%
        tih_rna_sample_00467_B23WHTKLT4_1_1
        130.5bp
        97.7%
        282.7M
        89.3%
        57%
        151bp
        294.0M
        tih_rna_sample_00467_B23WHTKLT4_1_2
        88.0%
        57%
        151bp
        294.0M
        tih_rna_sample_00467_B23WHTKLT4_1_trimmed_1
        89.4%
        58%
        127bp
        289.4M
        89.4%
        58%
        127bp
        289.4M
        tih_rna_sample_00467_B23WHTKLT4_1_trimmed_2
        89.3%
        58%
        127bp
        289.4M
        89.3%
        58%
        127bp
        289.4M
        tih_rna_sample_00467_B23WHTKLT4_2
        122.4bp
        115.0bp
        0.01%
        1.40%
        0.50%
        0.00%
        0.01%
        4.15%
        0.81%
        0.00%
        0.01
        9999000000
        142291644
        No
        0.00%
        590301006
        0.01%
        0.71%
        170721726
        0.91%
        0.94%
        0.07%
        0.06%
        154658499
        77791860
        12049033
        5049472
        245.03
        99.00
        0.68%
        0.50%
        0.97
        0.000
        562288476
        0.9427
        71.6%
        84.3%
        94.3%
        278.2M
        42.4%
        590.3M
        58.4%
        98.4%
        74.7%
        tih_rna_sample_00467_B23WHTKLT4_2_1
        122.4bp
        97.8%
        288.6M
        86.6%
        56%
        151bp
        311.5M
        tih_rna_sample_00467_B23WHTKLT4_2_2
        85.5%
        57%
        151bp
        311.5M
        tih_rna_sample_00467_B23WHTKLT4_2_trimmed_1
        86.3%
        58%
        117bp
        295.2M
        86.3%
        58%
        117bp
        295.2M
        tih_rna_sample_00467_B23WHTKLT4_2_trimmed_2
        86.5%
        58%
        117bp
        295.2M
        86.5%
        58%
        117bp
        295.2M
        tih_rna_sample_00473_B23WHTKLT4_1
        152.8bp
        139.0bp
        0.01%
        0.72%
        0.53%
        0.00%
        0.01%
        2.24%
        0.77%
        0.00%
        4.12
        9999000000
        136470681
        No
        0.00%
        590662262
        0.00%
        0.70%
        175175926
        0.93%
        0.96%
        0.05%
        0.04%
        163463520
        82676967
        9097434
        3020349
        319.03
        123.00
        0.67%
        0.53%
        0.97
        0.000
        573549228
        0.9551
        70.7%
        90.7%
        96.2%
        284.2M
        49.7%
        590.7M
        55.0%
        98.4%
        52.6%
        tih_rna_sample_00473_B23WHTKLT4_1_1
        152.8bp
        98.3%
        290.3M
        90.6%
        54%
        151bp
        482.0M
        tih_rna_sample_00473_B23WHTKLT4_1_2
        89.4%
        55%
        151bp
        482.0M
        tih_rna_sample_00473_B23WHTKLT4_1_trimmed_1
        87.7%
        54%
        147bp
        295.3M
        87.7%
        54%
        147bp
        295.3M
        tih_rna_sample_00473_B23WHTKLT4_1_trimmed_2
        87.9%
        55%
        147bp
        295.3M
        87.9%
        55%
        147bp
        295.3M
        tih_rna_sample_00473_B23WHTKLT4_2
        157.2bp
        144.0bp
        0.01%
        0.71%
        0.53%
        0.00%
        0.01%
        2.21%
        0.76%
        0.00%
        4.08
        9999000000
        194958916
        No
        0.00%
        591400834
        0.00%
        0.61%
        230009060
        0.94%
        0.97%
        0.05%
        0.03%
        216938401
        109772455
        10477697
        3269434
        440.43
        178.00
        0.70%
        0.58%
        0.97
        0.000
        574903540
        0.9542
        61.4%
        91.0%
        96.3%
        284.9M
        44.2%
        591.4M
        55.0%
        98.6%
        49.6%
        tih_rna_sample_00473_B23WHTKLT4_2_1
        157.2bp
        98.3%
        290.7M
        89.7%
        54%
        151bp
        526.3M
        tih_rna_sample_00473_B23WHTKLT4_2_2
        88.6%
        55%
        151bp
        526.3M
        tih_rna_sample_00473_B23WHTKLT4_2_trimmed_1
        86.1%
        54%
        151bp
        295.7M
        86.1%
        54%
        151bp
        295.7M
        tih_rna_sample_00473_B23WHTKLT4_2_trimmed_2
        86.2%
        55%
        151bp
        295.7M
        86.2%
        55%
        151bp
        295.7M
        tih_rna_sample_00479_B23WHTKLT4_1
        148.6bp
        137.0bp
        0.01%
        1.26%
        0.50%
        0.00%
        0.01%
        4.00%
        0.79%
        0.00%
        0.00
        9999000000
        155930250
        No
        0.00%
        591703898
        0.00%
        0.68%
        190795721
        0.93%
        0.96%
        0.06%
        0.04%
        177702036
        89886568
        10648899
        3730087
        341.53
        144.00
        0.71%
        0.57%
        0.97
        0.000
        574865287
        0.9519
        68.0%
        90.6%
        96.1%
        284.4M
        50.0%
        591.7M
        54.3%
        98.6%
        53.8%
        tih_rna_sample_00479_B23WHTKLT4_1_1
        148.6bp
        98.5%
        291.3M
        88.7%
        53%
        151bp
        374.1M
        tih_rna_sample_00479_B23WHTKLT4_1_2
        87.9%
        54%
        151bp
        374.1M
        tih_rna_sample_00479_B23WHTKLT4_1_trimmed_1
        87.3%
        54%
        147bp
        295.9M
        87.3%
        54%
        147bp
        295.9M
        tih_rna_sample_00479_B23WHTKLT4_1_trimmed_2
        87.6%
        54%
        147bp
        295.9M
        87.6%
        54%
        147bp
        295.9M
        tih_rna_sample_00479_B23WHTKLT4_2
        146.1bp
        133.0bp
        0.01%
        1.30%
        0.51%
        0.00%
        0.01%
        4.09%
        0.80%
        0.00%
        0.01
        9999000000
        232321953
        No
        0.00%
        375961592
        0.00%
        0.47%
        198468648
        0.95%
        0.97%
        0.05%
        0.03%
        187722582
        94881908
        9132494
        3058813
        363.91
        157.00
        0.72%
        0.58%
        0.97
        0.000
        364743882
        0.9614
        47.4%
        90.1%
        96.0%
        180.5M
        31.1%
        376.0M
        54.4%
        98.5%
        56.2%
        tih_rna_sample_00479_B23WHTKLT4_2_1
        146.1bp
        98.4%
        185.0M
        81.4%
        53%
        151bp
        190.8M
        tih_rna_sample_00479_B23WHTKLT4_2_2
        81.0%
        54%
        151bp
        190.8M
        tih_rna_sample_00479_B23WHTKLT4_2_trimmed_1
        81.5%
        54%
        142bp
        188.0M
        81.5%
        54%
        142bp
        188.0M
        tih_rna_sample_00479_B23WHTKLT4_2_trimmed_2
        82.1%
        54%
        142bp
        188.0M
        82.1%
        54%
        142bp
        188.0M
        tih_rna_sample_00480_B23WHTKLT4_1
        147.0bp
        135.0bp
        0.01%
        1.13%
        0.47%
        0.00%
        0.02%
        3.56%
        0.70%
        0.00%
        0.02
        9999000000
        147124207
        No
        0.00%
        565984516
        0.01%
        0.72%
        158086563
        0.91%
        0.95%
        0.07%
        0.05%
        144418081
        72981531
        10754705
        4072625
        267.88
        128.00
        0.72%
        0.55%
        0.97
        0.000
        547626924
        0.9342
        72.4%
        89.1%
        95.9%
        271.5M
        50.8%
        566.0M
        55.0%
        98.5%
        56.4%
        tih_rna_sample_00480_B23WHTKLT4_1_1
        147.0bp
        98.0%
        277.4M
        86.9%
        54%
        151bp
        287.4M
        tih_rna_sample_00480_B23WHTKLT4_1_2
        86.0%
        54%
        151bp
        287.4M
        tih_rna_sample_00480_B23WHTKLT4_1_trimmed_1
        87.0%
        54%
        142bp
        283.0M
        87.0%
        54%
        142bp
        283.0M
        tih_rna_sample_00480_B23WHTKLT4_1_trimmed_2
        87.3%
        55%
        142bp
        283.0M
        87.3%
        55%
        142bp
        283.0M
        tih_rna_sample_00480_B23WHTKLT4_2
        144.8bp
        134.0bp
        0.01%
        1.16%
        0.48%
        0.00%
        0.02%
        3.62%
        0.71%
        0.00%
        0.01
        9999000000
        225308965
        No
        0.00%
        590759296
        0.01%
        0.58%
        245219176
        0.93%
        0.96%
        0.06%
        0.04%
        228523974
        115431219
        13588661
        4971595
        430.82
        219.00
        0.76%
        0.64%
        0.97
        0.000
        571601059
        0.9318
        58.8%
        89.2%
        95.9%
        283.4M
        40.4%
        590.8M
        54.7%
        98.5%
        57.4%
        tih_rna_sample_00480_B23WHTKLT4_2_1
        144.8bp
        98.0%
        289.6M
        85.9%
        53%
        151bp
        365.3M
        tih_rna_sample_00480_B23WHTKLT4_2_2
        84.9%
        54%
        151bp
        365.3M
        tih_rna_sample_00480_B23WHTKLT4_2_trimmed_1
        84.4%
        54%
        142bp
        295.4M
        84.4%
        54%
        142bp
        295.4M
        tih_rna_sample_00480_B23WHTKLT4_2_trimmed_2
        84.7%
        54%
        142bp
        295.4M
        84.7%
        54%
        142bp
        295.4M

        Kallisto

        Kallisto is a program for quantifying abundances of transcripts from RNA-Seq data.DOI: 10.1038/nbt.3519.

        Created with MultiQC

        Picard

        Picard is a set of Java command line tools for manipulating high-throughput sequencing data.

        Mark Duplicates

        Number of reads, categorised by duplication state. Pair counts are doubled - see help text for details.

        The table in the Picard metrics file contains some columns referring read pairs and some referring to single reads.

        To make the numbers in this plot sum correctly, values referring to pairs are doubled according to the scheme below:

        • READS_IN_DUPLICATE_PAIRS = 2 * READ_PAIR_DUPLICATES
        • READS_IN_UNIQUE_PAIRS = 2 * (READ_PAIRS_EXAMINED - READ_PAIR_DUPLICATES)
        • READS_IN_UNIQUE_UNPAIRED = UNPAIRED_READS_EXAMINED - UNPAIRED_READ_DUPLICATES
        • READS_IN_DUPLICATE_PAIRS_OPTICAL = 2 * READ_PAIR_OPTICAL_DUPLICATES
        • READS_IN_DUPLICATE_PAIRS_NONOPTICAL = READS_IN_DUPLICATE_PAIRS - READS_IN_DUPLICATE_PAIRS_OPTICAL
        • READS_IN_DUPLICATE_UNPAIRED = UNPAIRED_READ_DUPLICATES
        • READS_UNMAPPED = UNMAPPED_READS
        Created with MultiQC

        Preseq

        Preseq estimates the complexity of a library, showing how many additional unique reads are sequenced for increasing total read count. A shallow curve indicates complexity saturation. The dashed line shows a perfectly complex library where total reads = unique reads.DOI: 10.1038/nmeth.2375.

        Complexity curve (molecule count)

        Note that the x-axis is trimmed at the point where all the datasets show 80% of their maximum y-value, to avoid ridiculous scales.

        Created with MultiQC

        RSeQC

        RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.DOI: 10.1093/bioinformatics/bts356.

        Read Distribution

        Read Distribution calculates how mapped reads are distributed over genome features.

        Created with MultiQC

        Inner Distance

        Inner Distance calculates the inner distance (or insert size) between two paired RNA reads. Note that this can be negative if fragments overlap.

        Created with MultiQC

        Read Duplication

        read_duplication.py calculates how many alignment positions have a certain number of exact duplicates. Note - plot truncated at 500 occurrences and binned.

        Created with MultiQC

        Junction Annotation

        Junction annotation compares detected splice junctions to a reference gene model. An RNA read can be spliced 2 or more times, each time is called a splicing event.

        Created with MultiQC

        Junction Saturation

        Junction Saturation counts the number of known splicing junctions that are observed in each dataset. If sequencing depth is sufficient, all (annotated) splice junctions should be rediscovered, resulting in a curve that reaches a plateau. Missing low abundance splice junctions can affect downstream analysis.

        Click a line to see the data side by side (as in the original RSeQC plot).

        Created with MultiQC

        Infer experiment

        Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

        Created with MultiQC

        Bam Stat

        All numbers reported in millions.

        Created with MultiQC

        STAR

        STAR is an ultrafast universal RNA-seq aligner.DOI: 10.1093/bioinformatics/bts635.

        Alignment Scores

        Created with MultiQC

        Gene Counts

        Statistics from results generated using --quantMode GeneCounts. The three tabs show counts for unstranded RNA-seq, counts for the 1st read strand aligned with RNA and counts for the 2nd read strand aligned with RNA.

        Created with MultiQC

        fastp

        fastp An ultra-fast all-in-one FASTQ preprocessor (QC, adapters, trimming, filtering, splitting...).DOI: 10.1093/bioinformatics/bty560.

        Filtered Reads

        Filtering statistics of sampled reads.

        Created with MultiQC

        Insert Sizes

        Insert size estimation of sampled reads.

        Created with MultiQC

        Sequence Quality

        Average sequencing quality over each base of all reads.

        Created with MultiQC

        GC Content

        Average GC content over each base of all reads.

        Created with MultiQC

        N content

        Average N content over each base of all reads.

        Created with MultiQC

        FastQC (raw)

        FastQC (raw) This section of the report shows FastQC results before adapter trimming.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Created with MultiQC

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Created with MultiQC

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Created with MultiQC

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Created with MultiQC

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Created with MultiQC

        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        Created with MultiQC

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Created with MultiQC

        Overrepresented sequences by sample

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Created with MultiQC

        Top overrepresented sequences

        Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

        Showing 20/20 rows and 3/3 columns.
        Overrepresented sequenceSamplesOccurrences% of all reads
        GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
        192
        208134299
        0.1946%
        GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
        4
        7901
        0.0000%
        TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC
        4
        6274
        0.0000%
        AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
        3
        2337
        0.0000%
        CATTGTGGTAATTGGCATGCTGGCCACGGGCCTCAGTCCCACCACCCGTA
        2
        75
        0.0000%
        ATGGGAATCCATTGGCGCTTCGGACAAGTCCTTCCCCTGCTCCGCCTTGA
        2
        70
        0.0000%
        AAAGTAATAGCTACTATTTTTTCTCTTTCAGTTGCAGGAGAAATAACAAG
        2
        72
        0.0000%
        GACATGAAGGTGCAGAGGGAGACAGATGAGGTGAAATAGAAGGTCATGCT
        2
        74
        0.0000%
        CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT
        2
        3636
        0.0000%
        GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGACGGCAAATCTGGG
        1
        437996
        0.0004%
        GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGACGGCAAATCTAGG
        1
        105132
        0.0001%
        GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATAGGTAGGATCTGGG
        1
        212407
        0.0002%
        GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACCTCAAATCTGGG
        1
        499330
        0.0005%
        GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTAACCTCAAATCTAGG
        1
        202349
        0.0002%
        AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTTGCGAAGTGAGTGTAGA
        1
        2755
        0.0000%
        GGGGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
        1
        2241
        0.0000%
        GGGGGGGGGAGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
        1
        2163
        0.0000%
        NGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
        1
        2001
        0.0000%
        GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC
        1
        777
        0.0000%
        GCTGCCGAGTGTTACAGCCCCCCCGGCAGC
        1
        174
        0.0000%

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Created with MultiQC

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        Created with MultiQC

        FastQC (trimmed)

        FastQC (trimmed) This section of the report shows FastQC results after adapter trimming.

        Sequence Counts

        Sequence counts for each sample. Duplicate read counts are an estimate only.

        This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

        You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        Created with MultiQC

        Sequence Quality Histograms

        The mean quality value across each base position in the read.

        To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

        Taken from the FastQC help:

        The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

        Created with MultiQC

        Per Sequence Quality Scores

        The number of reads with average quality scores. Shows if a subset of reads has poor quality.

        From the FastQC help:

        The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

        Created with MultiQC

        Per Base Sequence Content

        The proportion of each base position for which each of the four normal DNA bases has been called.

        To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

        To see the data as a line plot, as in the original FastQC graph, click on a sample track.

        From the FastQC help:

        Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

        In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

        It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

        Click a sample row to see a line plot for that dataset.
        Rollover for sample name
        Position: -
        %T: -
        %C: -
        %A: -
        %G: -

        Per Sequence GC Content

        The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

        From the FastQC help:

        This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

        In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

        An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

        Created with MultiQC

        Per Base N Content

        The percentage of base calls at each position for which an N was called.

        From the FastQC help:

        If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

        It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

        Created with MultiQC

        Sequence Length Distribution

        The distribution of fragment sizes (read lengths) found. See the FastQC help

        Created with MultiQC

        Sequence Duplication Levels

        The relative level of duplication found for every sequence.

        From the FastQC Help:

        In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

        Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

        The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

        In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

        Created with MultiQC

        Overrepresented sequences by sample

        The total amount of overrepresented sequences found in each library.

        FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

        Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

        From the FastQC Help:

        A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

        FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

        Created with MultiQC

        Top overrepresented sequences

        Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

        Showing 20/20 rows and 3/3 columns.
        Overrepresented sequenceSamplesOccurrences% of all reads
        GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG
        96
        81906447
        0.1668%
        GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
        2
        3918
        0.0000%
        TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC
        2
        3134
        0.0000%
        AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG
        2
        1439
        0.0000%
        CATTGTGGTAATTGGCATGCTGGCCACGGGCCTCAGTCCCACCACCCGTA
        2
        75
        0.0000%
        ATGGGAATCCATTGGCGCTTCGGACAAGTCCTTCCCCTGCTCCGCCTTGA
        2
        70
        0.0000%
        AAAGTAATAGCTACTATTTTTTCTCTTTCAGTTGCAGGAGAAATAACAAG
        2
        72
        0.0000%
        GACATGAAGGTGCAGAGGGAGACAGATGAGGTGAAATAGAAGGTCATGCT
        2
        74
        0.0000%
        GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC
        1
        777
        0.0000%
        GCTGCCGAGTGTTACAGCCCCCCCGGCAGC
        1
        174
        0.0000%
        GCTGCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC
        1
        88
        0.0000%
        CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC
        1
        56
        0.0000%
        TCTGGCTGCCGACAGTCAGGTTCTGCTGCAGAAGCACGTTCTCTATGCAG
        1
        49
        0.0000%
        GCGGGGGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
        1
        45
        0.0000%
        CTGCCATTGCAGGTTTGGATATCCTTCTGCCAGCAAAACCCAGCATGAAC
        1
        41
        0.0000%
        GCGGGGGAGAGAGAGAGAGAGAGGGCGC
        1
        41
        0.0000%
        CCCCGTCCTCCCCCCTCCCCGGGG
        1
        37
        0.0000%
        GTGCTGCTGCCGGGGGGGCTGTAACACTCGGGGGGCC
        1
        36
        0.0000%
        GGCATGTACTTGCCCCGCGCTGTGCTCGTGGATCTGGAGCTGGGCGCCAT
        1
        36
        0.0000%
        GGCAGCCGCAGGGGCTTCAGCACCACCTCGGCCATCACCCCGGCAGCTGG
        1
        35
        0.0000%

        Adapter Content

        The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

        Note that only samples with ≥ 0.1% adapter contamination are shown.

        There may be several lines per sample, as one is shown for each adapter detected in the file.

        From the FastQC Help:

        The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

        Created with MultiQC

        Status Checks

        Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

        It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

        Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

        In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

        Created with MultiQC

        Software Versions

        Software Versions lists versions of software tools extracted from file contents.

        GroupSoftwareVersion
        AGAT_CONVERTSPGFF2TSVagat1.2.0
        ARRIBAarriba2.4.0
        ARRIBA_FILTERfilter_arriba_main.py1.0
        pandas3.0.0
        pyyaml6.0.3
        ARRIBA_VISUALISATIONarriba2.4.0
        CANCER_INTRONS_TO_VCFpython3.11.0
        CONTAMINATION_SKIPPED_LOW_READScontamination_skipped_low_reads1.0.0
        CTATSPLICING_STARTOCANCERINTRONSctat-splicing0.0.3
        EXTRACT_VAF_FEATURESextract_vaf_features1.0.0
        python3.11.14
        FASTQCfastqc0.12.1
        FASTQC_FOR_FASTPfastqc0.12.1
        FUSIONINSPECTORSTAR-Fusion1.12.0
        FUSIONREPORTfusion_report2.1.5
        fusion_report DB retrievalnull
        GATK4_MARKDUPLICATESgatk44.4.0.0
        samtools1.17
        GTF2BEDperl5.26.2
        KALLISTO_QUANTkallisto0.51.1
        KALLISTO_TO_TX2GENE_QCexpression_qc_and_signatures1.0.0
        python3.11.0
        NGSCheckMATEngscheckmate1.0.0
        PREDICT_CONTAMINATIONpredict_contamination1.0.0
        python3.11.14
        PRESEQ_LCEXTRAPpreseq3.2.0
        PRESEQ_TO_MULTIQCpreseq_to_multiqc1.0.0
        python3.11.14
        RSEQC_BAMSTATrseqc5.0.2
        RSEQC_INFEREXPERIMENTrseqc5.0.2
        RSEQC_INNERDISTANCErseqc5.0.2
        RSEQC_JUNCTIONANNOTATIONrseqc5.0.2
        RSEQC_JUNCTIONSATURATIONrseqc5.0.2
        RSEQC_READDISTRIBUTIONrseqc5.0.2
        RSEQC_READDUPLICATIONrseqc5.0.2
        RSEQC_TO_MULTIQCpython3.11.14
        rseqc_to_multiqc1.0.0
        SAMTOOLS_INDEX_ALIGNMENT_CRAMsamtools1.17
        SAMTOOLS_INDEX_STAR_ALIGNMENTsamtools1.17
        SAMTOOLS_VIEW_ALIGNMENT_CRAMsamtools1.17
        SEQTOOL_TO_MULTIQCpython3.11.14
        seqtool_to_multiqc1.0.0
        STARFUSIONSTAR-Fusion1.12.0
        STARFUSION_FILTERboto3unknown
        filter_starfusion_main.py1.0
        numpy2.4.1
        pandas3.0.0
        pyyaml6.0.3
        STAR_ALIGNMENTgawk5.1.0
        samtools1.16.1
        star2.7.10a0
        VCF_COLLECTHGNC DB retrieval2025-01-22/23:32
        python3.11.14
        WorkflowNextflow25.10.2
        nf-core/rnafusion3.0.2
        fastpfastp0.23.4

        nf-core/rnafusion Methods Description

        Suggested text and references to use when describing pipeline usage within the methods section of a publication.

        Methods

        Data was processed using nf-core/rnafusion v3.0.2 of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

        The pipeline was executed with Nextflow v25.10.2 (Di Tommaso et al., 2017) with the following command:

        nextflow run /code/main.nf -output-dir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0020/B23WHTKLT4/nfcore_rnafusion__73d51683--20260607-225254' --project-dir /Users/anaik/Documents/Altera/rnafusion -profile docker,eks,std_run -name dev_rna_exp0020-73d51683 --rundir 's3://natera-labdata-rnd-dev-s3/illumina/20260515_LH00995_0042_B23WHTKLT4/' --bclconvert_samplesheet 's3://natera-labdata-rnd-dev-s3/illumina/20260515_LH00995_0042_B23WHTKLT4/bclconvert_samplesheet.csv' --flowcell_id 20260515_LH00995_0042_B23WHTKLT4 --outdir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0020/B23WHTKLT4/nfcore_rnafusion__73d51683--20260607-225254'

        References

        • Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
        • Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
        • Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
        • da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192
        Notes:
        • If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
        • The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
        • You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.

        nf-core/rnafusion Workflow Summary

        - this information is collected when the pipeline is started.

        Core Nextflow options

        runName
        dev_rna_exp0020-73d51683
        containerEngine
        docker
        launchDir
        /code
        workDir
        /natera-rnd-pltf-dev-nextflow-scratch-01/work
        projectDir
        /code
        userName
        nextflow
        profile
        docker,eks,std_run
        configFiles
        N/A

        Skip steps

        skip_picard_qc
        true

        Input/output options

        outdir
        s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0020/B23WHTKLT4/nfcore_rnafusion__73d51683--20260607-225254
        rundir
        s3://natera-labdata-rnd-dev-s3/illumina/20260515_LH00995_0042_B23WHTKLT4/
        flowcell_id
        20260515_LH00995_0042_B23WHTKLT4
        strand
        reverse
        bclconvert_samplesheet
        s3://natera-labdata-rnd-dev-s3/illumina/20260515_LH00995_0042_B23WHTKLT4/bclconvert_samplesheet.csv
        infer_sample_type_map
        true
        sample_type_map_positive_substring
        HD789
        genomes_base
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38
        read_length
        150
        arriba
        true
        arriba_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/
        arriba_ref_blacklist
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
        arriba_ref_cytobands
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
        arriba_ref_known_fusions
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
        arriba_ref_protein_domains
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
        arriba_gtf
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir//ref_annot.gtf
        ensembl_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/
        fusioncatcher_limitSjdbInsertNsj
        2000000
        fusioncatcher_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusioncatcher/human_v102/
        fusioninspector_limitSjdbInsertNsj
        1000000
        fusionreport_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusion_report_db/
        hgnc_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/hgnc_complete_set.txt
        hgnc_date
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/HGNC-DB-timestamp.txt
        starfusion
        true
        starfusion_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/
        starindex_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
        starindex_ensembl_ref
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
        tools_cutoff
        1

        Read trimming options

        fastp_trim
        true
        fastp_reads_to_process
        300000000
        adapter_fasta
        []

        Alignment compression options

        cram
        starfusion,arriba

        Reference genome options

        genome
        GRCh38
        fasta
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa
        fai
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai
        gtf
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf
        chrgtf
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf
        transcript
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz
        refflat
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat
        rrna_intervals
        s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.interval_list