[W::hts_idx_load3] The index file is older than the data file: 659_bGW-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17524525) splicing reads. 265991 splicing junctions. 185187 known splicing junctions. 80804 novel splicing junctions.
sampling 10% (35049050) splicing reads. 318151 splicing junctions. 195997 known splicing junctions. 122154 novel splicing junctions.
sampling 15% (52573575) splicing reads. 350023 splicing junctions. 201234 known splicing junctions. 148789 novel splicing junctions.
sampling 20% (70098100) splicing reads. 372455 splicing junctions. 204428 known splicing junctions. 168027 novel splicing junctions.
sampling 25% (87622625) splicing reads. 388969 splicing junctions. 206610 known splicing junctions. 182359 novel splicing junctions.
sampling 30% (105147150) splicing reads. 401695 splicing junctions. 208186 known splicing junctions. 193509 novel splicing junctions.
sampling 35% (122671675) splicing reads. 411717 splicing junctions. 209367 known splicing junctions. 202350 novel splicing junctions.
sampling 40% (140196200) splicing reads. 419711 splicing junctions. 210267 known splicing junctions. 209444 novel splicing junctions.
sampling 45% (157720725) splicing reads. 426231 splicing junctions. 210962 known splicing junctions. 215269 novel splicing junctions.
sampling 50% (175245250) splicing reads. 431730 splicing junctions. 211521 known splicing junctions. 220209 novel splicing junctions.
sampling 55% (192769775) splicing reads. 436256 splicing junctions. 211975 known splicing junctions. 224281 novel splicing junctions.
sampling 60% (210294300) splicing reads. 440091 splicing junctions. 212360 known splicing junctions. 227731 novel splicing junctions.
sampling 65% (227818825) splicing reads. 443457 splicing junctions. 212700 known splicing junctions. 230757 novel splicing junctions.
sampling 70% (245343350) splicing reads. 446181 splicing junctions. 212965 known splicing junctions. 233216 novel splicing junctions.
sampling 75% (262867875) splicing reads. 448497 splicing junctions. 213210 known splicing junctions. 235287 novel splicing junctions.
sampling 80% (280392400) splicing reads. 450453 splicing junctions. 213402 known splicing junctions. 237051 novel splicing junctions.
sampling 85% (297916925) splicing reads. 452085 splicing junctions. 213551 known splicing junctions. 238534 novel splicing junctions.
sampling 90% (315441450) splicing reads. 453381 splicing junctions. 213659 known splicing junctions. 239722 novel splicing junctions.
sampling 95% (332965975) splicing reads. 454392 splicing junctions. 213764 known splicing junctions. 240628 novel splicing junctions.
sampling 100% (350490501) splicing reads. 455207 splicing junctions. 213831 known splicing junctions. 241376 novel splicing junctions.