[W::hts_idx_load3] The index file is older than the data file: 659_cAd-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (15650982) splicing reads. 250858 splicing junctions. 178513 known splicing junctions. 72345 novel splicing junctions.
sampling 10% (31301964) splicing reads. 288503 splicing junctions. 187186 known splicing junctions. 101317 novel splicing junctions.
sampling 15% (46952946) splicing reads. 308573 splicing junctions. 190992 known splicing junctions. 117581 novel splicing junctions.
sampling 20% (62603928) splicing reads. 321362 splicing junctions. 193174 known splicing junctions. 128188 novel splicing junctions.
sampling 25% (78254911) splicing reads. 329899 splicing junctions. 194543 known splicing junctions. 135356 novel splicing junctions.
sampling 30% (93905893) splicing reads. 336274 splicing junctions. 195532 known splicing junctions. 140742 novel splicing junctions.
sampling 35% (109556875) splicing reads. 341079 splicing junctions. 196229 known splicing junctions. 144850 novel splicing junctions.
sampling 40% (125207857) splicing reads. 344947 splicing junctions. 196785 known splicing junctions. 148162 novel splicing junctions.
sampling 45% (140858839) splicing reads. 348048 splicing junctions. 197186 known splicing junctions. 150862 novel splicing junctions.
sampling 50% (156509822) splicing reads. 350614 splicing junctions. 197500 known splicing junctions. 153114 novel splicing junctions.
sampling 55% (172160804) splicing reads. 352830 splicing junctions. 197761 known splicing junctions. 155069 novel splicing junctions.
sampling 60% (187811786) splicing reads. 354699 splicing junctions. 198002 known splicing junctions. 156697 novel splicing junctions.
sampling 65% (203462768) splicing reads. 356323 splicing junctions. 198219 known splicing junctions. 158104 novel splicing junctions.
sampling 70% (219113750) splicing reads. 357668 splicing junctions. 198387 known splicing junctions. 159281 novel splicing junctions.
sampling 75% (234764733) splicing reads. 358741 splicing junctions. 198497 known splicing junctions. 160244 novel splicing junctions.
sampling 80% (250415715) splicing reads. 359688 splicing junctions. 198618 known splicing junctions. 161070 novel splicing junctions.
sampling 85% (266066697) splicing reads. 360448 splicing junctions. 198707 known splicing junctions. 161741 novel splicing junctions.
sampling 90% (281717679) splicing reads. 361003 splicing junctions. 198779 known splicing junctions. 162224 novel splicing junctions.
sampling 95% (297368661) splicing reads. 361420 splicing junctions. 198815 known splicing junctions. 162605 novel splicing junctions.
sampling 100% (313019644) splicing reads. 361761 splicing junctions. 198853 known splicing junctions. 162908 novel splicing junctions.