[W::hts_idx_load3] The index file is older than the data file: 659_bNS-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (18010813) splicing reads. 263573 splicing junctions. 188552 known splicing junctions. 75021 novel splicing junctions.
sampling 10% (36021627) splicing reads. 307286 splicing junctions. 198465 known splicing junctions. 108821 novel splicing junctions.
sampling 15% (54032441) splicing reads. 332538 splicing junctions. 203118 known splicing junctions. 129420 novel splicing junctions.
sampling 20% (72043255) splicing reads. 349132 splicing junctions. 205713 known splicing junctions. 143419 novel splicing junctions.
sampling 25% (90054069) splicing reads. 360814 splicing junctions. 207422 known splicing junctions. 153392 novel splicing junctions.
sampling 30% (108064883) splicing reads. 369819 splicing junctions. 208638 known splicing junctions. 161181 novel splicing junctions.
sampling 35% (126075697) splicing reads. 376721 splicing junctions. 209561 known splicing junctions. 167160 novel splicing junctions.
sampling 40% (144086511) splicing reads. 382250 splicing junctions. 210266 known splicing junctions. 171984 novel splicing junctions.
sampling 45% (162097325) splicing reads. 386609 splicing junctions. 210786 known splicing junctions. 175823 novel splicing junctions.
sampling 50% (180108139) splicing reads. 390164 splicing junctions. 211228 known splicing junctions. 178936 novel splicing junctions.
sampling 55% (198118952) splicing reads. 393229 splicing junctions. 211588 known splicing junctions. 181641 novel splicing junctions.
sampling 60% (216129766) splicing reads. 395779 splicing junctions. 211888 known splicing junctions. 183891 novel splicing junctions.
sampling 65% (234140580) splicing reads. 398128 splicing junctions. 212152 known splicing junctions. 185976 novel splicing junctions.
sampling 70% (252151394) splicing reads. 400092 splicing junctions. 212377 known splicing junctions. 187715 novel splicing junctions.
sampling 75% (270162208) splicing reads. 401753 splicing junctions. 212565 known splicing junctions. 189188 novel splicing junctions.
sampling 80% (288173022) splicing reads. 403106 splicing junctions. 212695 known splicing junctions. 190411 novel splicing junctions.
sampling 85% (306183836) splicing reads. 404333 splicing junctions. 212839 known splicing junctions. 191494 novel splicing junctions.
sampling 90% (324194650) splicing reads. 405275 splicing junctions. 212937 known splicing junctions. 192338 novel splicing junctions.
sampling 95% (342205464) splicing reads. 406047 splicing junctions. 213020 known splicing junctions. 193027 novel splicing junctions.
sampling 100% (360216278) splicing reads. 406700 splicing junctions. 213074 known splicing junctions. 193626 novel splicing junctions.