[W::hts_idx_load3] The index file is older than the data file: 659_cRB-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (13729240) splicing reads. 230175 splicing junctions. 173308 known splicing junctions. 56867 novel splicing junctions.
sampling 10% (27458480) splicing reads. 261855 splicing junctions. 181572 known splicing junctions. 80283 novel splicing junctions.
sampling 15% (41187720) splicing reads. 279058 splicing junctions. 185298 known splicing junctions. 93760 novel splicing junctions.
sampling 20% (54916960) splicing reads. 289883 splicing junctions. 187363 known splicing junctions. 102520 novel splicing junctions.
sampling 25% (68646200) splicing reads. 297529 splicing junctions. 188704 known splicing junctions. 108825 novel splicing junctions.
sampling 30% (82375440) splicing reads. 303077 splicing junctions. 189701 known splicing junctions. 113376 novel splicing junctions.
sampling 35% (96104680) splicing reads. 307375 splicing junctions. 190364 known splicing junctions. 117011 novel splicing junctions.
sampling 40% (109833920) splicing reads. 310679 splicing junctions. 190888 known splicing junctions. 119791 novel splicing junctions.
sampling 45% (123563160) splicing reads. 313366 splicing junctions. 191291 known splicing junctions. 122075 novel splicing junctions.
sampling 50% (137292400) splicing reads. 315628 splicing junctions. 191651 known splicing junctions. 123977 novel splicing junctions.
sampling 55% (151021640) splicing reads. 317455 splicing junctions. 191915 known splicing junctions. 125540 novel splicing junctions.
sampling 60% (164750880) splicing reads. 319013 splicing junctions. 192114 known splicing junctions. 126899 novel splicing junctions.
sampling 65% (178480120) splicing reads. 320300 splicing junctions. 192284 known splicing junctions. 128016 novel splicing junctions.
sampling 70% (192209360) splicing reads. 321441 splicing junctions. 192427 known splicing junctions. 129014 novel splicing junctions.
sampling 75% (205938600) splicing reads. 322374 splicing junctions. 192551 known splicing junctions. 129823 novel splicing junctions.
sampling 80% (219667840) splicing reads. 323154 splicing junctions. 192656 known splicing junctions. 130498 novel splicing junctions.
sampling 85% (233397080) splicing reads. 323793 splicing junctions. 192749 known splicing junctions. 131044 novel splicing junctions.
sampling 90% (247126320) splicing reads. 324243 splicing junctions. 192812 known splicing junctions. 131431 novel splicing junctions.
sampling 95% (260855560) splicing reads. 324562 splicing junctions. 192847 known splicing junctions. 131715 novel splicing junctions.
sampling 100% (274584800) splicing reads. 324745 splicing junctions. 192868 known splicing junctions. 131877 novel splicing junctions.