[W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	57672184
Known Splicing Events:	57087450
Partial Novel Splicing Events:	351396
Novel Splicing Events:	202794
Filtered Splicing Events:	30544

Total splicing  Junctions:	157492
Known Splicing Junctions:	138452
Partial Novel Splicing Junctions:	10431
Novel Splicing Junctions:	8609

===================================================================
Create BED file ...
Create Interact file ...