[W::hts_idx_load3] The index file is older than the data file: 659_cfy-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	361502058
Known Splicing Events:	358593885
Partial Novel Splicing Events:	1523377
Novel Splicing Events:	1222216
Filtered Splicing Events:	162580

Total splicing  Junctions:	486507
Known Splicing Junctions:	252319
Partial Novel Splicing Junctions:	113188
Novel Splicing Junctions:	121000

===================================================================
Create BED file ...
Create Interact file ...