[W::hts_idx_load3] The index file is older than the data file: 659_bTo-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (19596568) splicing reads. 267536 splicing junctions. 182383 known splicing junctions. 85153 novel splicing junctions.
sampling 10% (39193136) splicing reads. 327650 splicing junctions. 194646 known splicing junctions. 133004 novel splicing junctions.
sampling 15% (58789705) splicing reads. 367892 splicing junctions. 200844 known splicing junctions. 167048 novel splicing junctions.
sampling 20% (78386273) splicing reads. 397372 splicing junctions. 204792 known splicing junctions. 192580 novel splicing junctions.
sampling 25% (97982842) splicing reads. 420481 splicing junctions. 207417 known splicing junctions. 213064 novel splicing junctions.
sampling 30% (117579410) splicing reads. 438770 splicing junctions. 209369 known splicing junctions. 229401 novel splicing junctions.
sampling 35% (137175978) splicing reads. 453659 splicing junctions. 210892 known splicing junctions. 242767 novel splicing junctions.
sampling 40% (156772547) splicing reads. 466202 splicing junctions. 212087 known splicing junctions. 254115 novel splicing junctions.
sampling 45% (176369115) splicing reads. 476680 splicing junctions. 213065 known splicing junctions. 263615 novel splicing junctions.
sampling 50% (195965684) splicing reads. 485794 splicing junctions. 213943 known splicing junctions. 271851 novel splicing junctions.
sampling 55% (215562252) splicing reads. 493398 splicing junctions. 214597 known splicing junctions. 278801 novel splicing junctions.
sampling 60% (235158820) splicing reads. 500057 splicing junctions. 215161 known splicing junctions. 284896 novel splicing junctions.
sampling 65% (254755389) splicing reads. 505694 splicing junctions. 215618 known splicing junctions. 290076 novel splicing junctions.
sampling 70% (274351957) splicing reads. 510737 splicing junctions. 216055 known splicing junctions. 294682 novel splicing junctions.
sampling 75% (293948526) splicing reads. 515090 splicing junctions. 216432 known splicing junctions. 298658 novel splicing junctions.
sampling 80% (313545094) splicing reads. 518794 splicing junctions. 216743 known splicing junctions. 302051 novel splicing junctions.
sampling 85% (333141662) splicing reads. 521898 splicing junctions. 217004 known splicing junctions. 304894 novel splicing junctions.
sampling 90% (352738231) splicing reads. 524461 splicing junctions. 217196 known splicing junctions. 307265 novel splicing junctions.
sampling 95% (372334799) splicing reads. 526622 splicing junctions. 217354 known splicing junctions. 309268 novel splicing junctions.
sampling 100% (391931368) splicing reads. 528246 splicing junctions. 217466 known splicing junctions. 310780 novel splicing junctions.