[W::hts_idx_load3] The index file is older than the data file: 659_cIX-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17367939) splicing reads. 249828 splicing junctions. 177897 known splicing junctions. 71931 novel splicing junctions.
sampling 10% (34735878) splicing reads. 294601 splicing junctions. 188051 known splicing junctions. 106550 novel splicing junctions.
sampling 15% (52103817) splicing reads. 321868 splicing junctions. 193077 known splicing junctions. 128791 novel splicing junctions.
sampling 20% (69471756) splicing reads. 340252 splicing junctions. 196068 known splicing junctions. 144184 novel splicing junctions.
sampling 25% (86839695) splicing reads. 353721 splicing junctions. 197982 known splicing junctions. 155739 novel splicing junctions.
sampling 30% (104207634) splicing reads. 364019 splicing junctions. 199456 known splicing junctions. 164563 novel splicing junctions.
sampling 35% (121575573) splicing reads. 371986 splicing junctions. 200419 known splicing junctions. 171567 novel splicing junctions.
sampling 40% (138943512) splicing reads. 378580 splicing junctions. 201201 known splicing junctions. 177379 novel splicing junctions.
sampling 45% (156311451) splicing reads. 383904 splicing junctions. 201864 known splicing junctions. 182040 novel splicing junctions.
sampling 50% (173679390) splicing reads. 388390 splicing junctions. 202412 known splicing junctions. 185978 novel splicing junctions.
sampling 55% (191047329) splicing reads. 392093 splicing junctions. 202841 known splicing junctions. 189252 novel splicing junctions.
sampling 60% (208415268) splicing reads. 395146 splicing junctions. 203206 known splicing junctions. 191940 novel splicing junctions.
sampling 65% (225783207) splicing reads. 397884 splicing junctions. 203533 known splicing junctions. 194351 novel splicing junctions.
sampling 70% (243151146) splicing reads. 400156 splicing junctions. 203768 known splicing junctions. 196388 novel splicing junctions.
sampling 75% (260519085) splicing reads. 402139 splicing junctions. 204010 known splicing junctions. 198129 novel splicing junctions.
sampling 80% (277887024) splicing reads. 403740 splicing junctions. 204160 known splicing junctions. 199580 novel splicing junctions.
sampling 85% (295254963) splicing reads. 405070 splicing junctions. 204314 known splicing junctions. 200756 novel splicing junctions.
sampling 90% (312622902) splicing reads. 406130 splicing junctions. 204414 known splicing junctions. 201716 novel splicing junctions.
sampling 95% (329990841) splicing reads. 407020 splicing junctions. 204499 known splicing junctions. 202521 novel splicing junctions.
sampling 100% (347358781) splicing reads. 407710 splicing junctions. 204569 known splicing junctions. 203141 novel splicing junctions.