Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/889381dd66e1bf90174760f31776b9/659_bQZ-T1-TRNA-1_B23WHTKLT4_1_2.fastp.fastq.gz Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_annot.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/de/4678714b8e162a7dbf4c6cced3c370/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.star.idx Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/de/4678714b8e162a7dbf4c6cced3c370/.command.run Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/76/889381dd66e1bf90174760f31776b9/659_bQZ-T1-TRNA-1_B23WHTKLT4_1_1.fastp.fastq.gz ==> STAGING COMPLETE (6 inputs) STAR --genomeDir ref_genome.fa.star.idx --readFilesIn input1/659_bQZ-T1-TRNA-1_B23WHTKLT4_1_1.fastp.fastq.gz input2/659_bQZ-T1-TRNA-1_B23WHTKLT4_1_2.fastp.fastq.gz --runThreadN 24 --outFileNamePrefix 659_bQZ-T1-TRNA-1_B23WHTKLT4_1. --sjdbGTFfile ref_annot.gtf --outSAMattrRGline ID:659_bQZ-T1-TRNA-1_B23WHTKLT4_1 SM:659_bQZ-T1-TRNA-1_B23WHTKLT4_1 --twopassMode None --chimSegmentMin 12 --chimJunctionOverhangMin 5 --chimMultimapNmax 50 --chimScoreJunctionNonGTAG -4 --chimScoreSeparation 1 --alignIntronMax 140000 --outReadsUnmapped None --readFilesCommand zcat --outSAMtype BAM SortedByCoordinate --outSAMstrandField intronMotif --outSAMunmapped Within --chimOutJunctionFormat 1 --alignSJDBoverhangMin 10 --alignMatesGapMax 100000 --alignSJstitchMismatchNmax 5 -1 5 5 --chimMultimapScoreRange 3 --chimNonchimScoreDropMin 10 --peOverlapNbasesMin 12 --peOverlapMMp 0.1 --alignInsertionFlush Right --alignSplicedMateMapLmin 30 --alignSplicedMateMapLminOverLmate 0.5 --quantMode GeneCounts --runRNGseed 1 --chimOutType Junctions WithinBAM --limitBAMsortRAM 38654705664 STAR version: 2.7.10a compiled: 2022-01-14T18:50:00-05:00 :/home/dobin/data/STAR/STARcode/STAR.master/source Jun 08 07:39:54 ..... started STAR run Jun 08 07:39:54 ..... loading genome Jun 08 07:40:18 ..... processing annotations GTF Jun 08 07:40:28 ..... inserting junctions into the genome indices Jun 08 07:41:43 ..... started mapping Jun 08 08:54:46 ..... finished mapping Jun 08 08:54:49 ..... started sorting BAM Jun 08 08:59:03 ..... finished successfully