Done. -parsing GTF file: /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.8Y0BsPg7Kt/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1_1.fastp.fastq.gz /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00150_B23WHTKLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00150_B23WHTKLT4_1.star.sortedByCoord.out.bam 10:58:37 : INFO : Done. -parsing GTF file: /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.gtf -parsing /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] -done parsing /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'num genes matched < 2' => 6, 'per_id < 96' => 550, 'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 4, 'excessive soft clipping' => 1539, 'num_hits: 6 != num_counted_on_fusion_contigs 4 ' => 440, 'seq-similar region overlap' => 190, ' ** passed ** ' => 1614, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 14 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: AP000347.1--IGLL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: PCDHA3--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 372, 'seq similar region alignment' => 10164 }; EM: Starting log likelihood: -967.193665 EM: Round [1] log likelihood: -966.888224 EM: Round [2] log likelihood: -966.818459 EM: Round [3] log likelihood: -966.754080 EM: Round [4] log likelihood: -966.694521 EM: Round [5] log likelihood: -966.639336 EM: Round [6] log likelihood: -966.588133 EM: Round [7] log likelihood: -966.540563 EM: Round [8] log likelihood: -966.496319 EM: Round [9] log likelihood: -966.455124 EM: Round [10] log likelihood: -966.416729 EM: Round [11] log likelihood: -966.380914 EM: Round [12] log likelihood: -966.347476 EM: Round [13] log likelihood: -966.316235 EM: Round [14] log likelihood: -966.287025 EM: Round [15] log likelihood: -966.259696 EM: Round [16] log likelihood: -966.234113 EM: Round [17] log likelihood: -966.210149 EM: Round [18] log likelihood: -966.187691 EM: Round [19] log likelihood: -966.166634 EM: Round [20] log likelihood: -966.146882 EM: Round [21] log likelihood: -966.128346 EM: Round [22] log likelihood: -966.110944 EM: Round [23] log likelihood: -966.094601 EM: Round [24] log likelihood: -966.079247 EM: Round [25] log likelihood: -966.064818 EM: Round [26] log likelihood: -966.051254 EM: Round [27] log likelihood: -966.038500 EM: Round [28] log likelihood: -966.026503 EM: Round [29] log likelihood: -966.015218 EM: Round [30] log likelihood: -966.004598 EM: Round [31] log likelihood: -965.994602 EM: Round [32] log likelihood: -965.985192 EM: Round [33] log likelihood: -965.976332 EM: Round [34] log likelihood: -965.967988 EM: Round [35] log likelihood: -965.960129 EM: Round [36] log likelihood: -965.952726 EM: Round [37] log likelihood: -965.945750 EM: Round [38] log likelihood: -965.939177 EM: Round [39] log likelihood: -965.932982 EM: Round [40] log likelihood: -965.927143 EM: Round [41] log likelihood: -965.921639 EM: Round [42] log likelihood: -965.916450 EM: Round [43] log likelihood: -965.911558 EM: Round [44] log likelihood: -965.906944 EM: Round [45] log likelihood: -965.902594 EM: Round [46] log likelihood: -965.898491 EM: Round [47] log likelihood: -965.894621 EM: Round [48] log likelihood: -965.890970 EM: Round [49] log likelihood: -965.887527 EM: Round [50] log likelihood: -965.884278 EM: Round [51] log likelihood: -965.881213 EM: Round [52] log likelihood: -965.878321 EM: Round [53] log likelihood: -965.875593 EM: Round [54] log likelihood: -965.873018 EM: Round [55] log likelihood: -965.870588 EM: Round [56] log likelihood: -965.868295 EM: Round [57] log likelihood: -965.866131 EM: Round [58] log likelihood: -965.864089 EM: Round [59] log likelihood: -965.862162 EM: Round [60] log likelihood: -965.860343 EM: Round [61] log likelihood: -965.858625 EM: Round [62] log likelihood: -965.857004 EM: Round [63] log likelihood: -965.855474 EM: Round [64] log likelihood: -965.854030 EM: Round [65] log likelihood: -965.852667 EM: Round [66] log likelihood: -965.851380 EM: Round [67] log likelihood: -965.850164 EM: Round [68] log likelihood: -965.849017 EM: Round [69] log likelihood: -965.847934 EM: Round [70] log likelihood: -965.846912 EM: Round [71] log likelihood: -965.845946 EM: Round [72] log likelihood: -965.845035 EM: Round [73] log likelihood: -965.844174 EM: Round [74] log likelihood: -965.843362 EM: Round [75] log likelihood: -965.842595 EM: Round [76] log likelihood: -965.841870 EM: Round [77] log likelihood: -965.841186 EM: Round [78] log likelihood: -965.840541 EM: Round [79] log likelihood: -965.839931 EM: Round [80] log likelihood: -965.839355 EM: Round [81] log likelihood: -965.838811 EM: Round [82] log likelihood: -965.838298 EM: Round [83] log likelihood: -965.837813 EM: Round [84] log likelihood: -965.837355 EM: Round [85] log likelihood: -965.836923 EM: Round [86] log likelihood: -965.836514 EM: Round [87] log likelihood: -965.836129 EM: Round [88] log likelihood: -965.835765 EM: Round [89] log likelihood: -965.835421 EM: Round [90] log likelihood: -965.835096 EM: Round [91] log likelihood: -965.834790 EM: Round [92] log likelihood: -965.834500 EM: Round [93] log likelihood: -965.834227 EM: Round [94] log likelihood: -965.833969 EM: Round [95] log likelihood: -965.833725 EM: Round [96] log likelihood: -965.833495 EM: Round [97] log likelihood: -965.833277 EM: Round [98] log likelihood: -965.833072 EM: Round [99] log likelihood: -965.832878 EM: Round [100] log likelihood: -965.832695 EM: Round [101] log likelihood: -965.832522 EM: Round [102] log likelihood: -965.832358 EM: Round [103] log likelihood: -965.832204 EM: Round [104] log likelihood: -965.832058 EM: Round [105] log likelihood: -965.831921 EM: Round [106] log likelihood: -965.831791 EM: Round [107] log likelihood: -965.831668 EM: Round [108] log likelihood: -965.831552 EM: Round [109] log likelihood: -965.831443 EM: Round [110] log likelihood: -965.831339 EM: Round [111] log likelihood: -965.831242 EM: Stopping iterations at round 111 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam into /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam.read_coords > /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam.frag_coords > /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.8Y0BsPg7Kt/fi_workdir/tih_rna_sample_00150_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -681.783093 EM: Round [1] log likelihood: -681.782283 EM: Round [2] log likelihood: -681.782282 EM: Stopping iterations at round 2 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1_1.fastp.fastq.gz: 247680642 Warning - not locating file: /tmp/nxf.8Y0BsPg7Kt/tih_rna_sample_00150_B23WHTKLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000272578.5] WARNING, no entry stored in dbm for [ENSG00000272578.5] WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] ls: cannot access 'IGV_inputs': No such file or directory