Done. -parsing GTF file: /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.ULdfDeyq3O/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2_1.fastp.fastq.gz /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00124_B23WHTKLT4_2.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00124_B23WHTKLT4_2.star.sortedByCoord.out.bam 10:34:13 : INFO : Done. -parsing GTF file: /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.gtf -parsing /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] [770000] [780000] [790000] [800000] -done parsing /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'exons hit < 2' => 15, 'num_hits: 5 != num_counted_on_fusion_contigs 1 ' => 2, 'seq-similar region overlap' => 86, 'num_hits: 6 != num_counted_on_fusion_contigs 5 ' => 5, 'num_hits: 7 != num_counted_on_fusion_contigs 4 ' => 12, 'per_id < 96' => 2429, 'num genes matched < 2' => 58, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 5172, 'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 1132, 'num_hits: 7 != num_counted_on_fusion_contigs 5 ' => 5, 'low complexity anchor region' => 3, 'num_hits: 6 != num_counted_on_fusion_contigs 3 ' => 3, 'num_hits: 7 != num_counted_on_fusion_contigs 6 ' => 6, 'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 284, 'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 2932, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 27, 'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 276, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 1854, 'excessive soft clipping' => 4565, ' ** passed ** ' => 1439, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 2554, 'small anchor length' => 4, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 1192 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] -fusion SPANNING read extraction for scaff: ANKRD30A--ARL6IP1P2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] -fusion SPANNING read extraction for scaff: BLCAP--SEZ6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] -fusion SPANNING read extraction for scaff: C6orf89--DDR1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] -fusion SPANNING read extraction for scaff: DDX11--IPO8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] -fusion SPANNING read extraction for scaff: KLRC4--KLRK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] -fusion SPANNING read extraction for scaff: NF1--TMEM161A [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] -fusion SPANNING read extraction for scaff: PILRA--PILRB [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] [123000] [124000] [125000] [126000] [127000] [128000] [129000] [130000] [131000] [132000] [133000] [134000] -fusion SPANNING read extraction for scaff: PLEKHM1--MAP4K4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] -fusion SPANNING read extraction for scaff: RIC1--INSL4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] -fusion SPANNING read extraction for scaff: STX16--NPEPL1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1 [1000] [2000] [3000] [4000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: TPST1--AC073210.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] [84000] [85000] [86000] [87000] [88000] [89000] [90000] [91000] [92000] [93000] [94000] [95000] [96000] [97000] [98000] [99000] [100000] [101000] [102000] [103000] [104000] [105000] [106000] [107000] [108000] [109000] [110000] [111000] [112000] [113000] [114000] [115000] [116000] [117000] [118000] [119000] [120000] [121000] [122000] -fusion SPANNING read extraction for scaff: XRN1--ATR [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] -fusion SPANNING read extraction for scaff: ZNF692--GCSAML -filtered reads reasons: $VAR1 = { 'seq similar region alignment' => 24570, 'lacks exon overlap' => 8636 }; EM: Starting log likelihood: -2165.317460 EM: Round [1] log likelihood: -2165.081052 EM: Round [2] log likelihood: -2165.080230 EM: Round [3] log likelihood: -2165.080222 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam into /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam.read_coords CMD: touch /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam.read_coords > /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam.frag_coords > /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.ULdfDeyq3O/fi_workdir/tih_rna_sample_00124_B23WHTKLT4_2.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.post_blast_and_promiscuity_filter -done, see /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -2107.458209 EM: Round [1] log likelihood: -2107.221801 EM: Round [2] log likelihood: -2107.220979 EM: Round [3] log likelihood: -2107.220971 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2_1.fastp.fastq.gz: 225028134 Warning - not locating file: /tmp/nxf.ULdfDeyq3O/tih_rna_sample_00124_B23WHTKLT4_2.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000280136.2] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000227113.2] WARNING, no entry stored in dbm for [ENSG00000227113.2] ls: cannot access 'IGV_inputs': No such file or directory