+ mkdir 659_ciJ-T1-TRNA-1_B23WHTKLT4_1 + all_qc -bam 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 659_ciJ-T1-TRNA-1_B23WHTKLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai 147333it [00:00, 1472794.16it/s] 307730it [00:00, 1549839.24it/s] 472691it [00:00, 1595169.70it/s] 637395it [00:00, 1615605.40it/s] 802331it [00:00, 1627744.53it/s] 970152it [00:00, 1644872.51it/s] 1134640it [00:00, 1643901.73it/s] 1300313it [00:00, 1647870.52it/s] 1468545it [00:00, 1658613.11it/s] 1635758it [00:01, 1662759.77it/s] 1802035it [00:01, 1659494.56it/s] 1967987it [00:01, 1656483.28it/s] 2135213it [00:01, 1661226.88it/s] 2301339it [00:01, 1658824.63it/s] 2467224it [00:01, 1651881.50it/s] 2635729it [00:01, 1661800.06it/s] 2802124it [00:01, 1662421.25it/s] 2968381it [00:01, 1662442.55it/s] 3134762it [00:01, 1662482.25it/s] 3301014it [00:02, 1642243.01it/s] 3470007it [00:02, 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1664226.37it/s] 8475426it [00:05, 1666106.96it/s] 8643686it [00:05, 1671024.99it/s] 8811738it [00:05, 1673747.22it/s] 8980267it [00:05, 1677183.31it/s] 9148479it [00:05, 1678635.01it/s] 9316345it [00:05, 1672662.74it/s] 9483618it [00:05, 1669243.82it/s] 9650548it [00:05, 1656138.55it/s] 9816378it [00:05, 1656613.05it/s] 9982056it [00:06, 1654229.04it/s] 10149063it [00:06, 1658829.52it/s] 10316142it [00:06, 1662380.57it/s] 10483946it [00:06, 1667041.02it/s] 10651772it [00:06, 1670366.70it/s] 10821469it [00:06, 1678147.65it/s] 10989289it [00:06, 1659504.56it/s] 11155756it [00:06, 1661017.98it/s] 11322329it [00:06, 1662397.64it/s] 11488595it [00:06, 1656785.04it/s] 11654294it [00:07, 1649189.67it/s] 11820667it [00:07, 1653494.83it/s] 11988749it [00:07, 1661624.31it/s] 12154927it [00:07, 1659618.89it/s] 12320900it [00:07, 1658760.95it/s] 12486784it [00:07, 1656201.26it/s] 12652410it [00:07, 1653441.27it/s] 12817759it [00:07, 1645125.46it/s] 12982545it [00:07, 1645774.18it/s] 13147130it [00:07, 1642783.00it/s] 13312346it [00:08, 1645557.85it/s] 13477695it [00:08, 1647899.23it/s][W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai 13643719it [00:08, 1651527.06it/s] 13810954it [00:08, 1657741.48it/s] 13976732it [00:08, 1655250.93it/s] 14142261it [00:08, 1639684.66it/s] 14307657it [00:08, 1643907.26it/s] 14472981it [00:08, 1646665.15it/s] 14637826it [00:08, 1647173.17it/s] 14777101it [00:08, 1656198.83it/s] [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md.bai + merge_qc -folders 659_ciJ-T1-TRNA-1_B23WHTKLT4_1/659_ciJ-T1-TRNA-1_B23WHTKLT4_1.md -output 659_ciJ-T1-TRNA-1_B23WHTKLT4_1/659_ciJ-T1-TRNA-1_B23WHTKLT4_1_full_basic_stats.tsv + head -n1 659_ciJ-T1-TRNA-1_B23WHTKLT4_1/659_ciJ-T1-TRNA-1_B23WHTKLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 659_ciJ-T1-TRNA-1_B23WHTKLT4_1/659_ciJ-T1-TRNA-1_B23WHTKLT4_1_full_basic_stats.tsv