[W::hts_idx_load3] The index file is older than the data file: 659_c95-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17501782) splicing reads. 274772 splicing junctions. 188964 known splicing junctions. 85808 novel splicing junctions.
sampling 10% (35003564) splicing reads. 335612 splicing junctions. 201369 known splicing junctions. 134243 novel splicing junctions.
sampling 15% (52505346) splicing reads. 377137 splicing junctions. 207934 known splicing junctions. 169203 novel splicing junctions.
sampling 20% (70007128) splicing reads. 407804 splicing junctions. 211971 known splicing junctions. 195833 novel splicing junctions.
sampling 25% (87508910) splicing reads. 432316 splicing junctions. 214796 known splicing junctions. 217520 novel splicing junctions.
sampling 30% (105010692) splicing reads. 452239 splicing junctions. 216960 known splicing junctions. 235279 novel splicing junctions.
sampling 35% (122512473) splicing reads. 468609 splicing junctions. 218625 known splicing junctions. 249984 novel splicing junctions.
sampling 40% (140014256) splicing reads. 482226 splicing junctions. 219934 known splicing junctions. 262292 novel splicing junctions.
sampling 45% (157516038) splicing reads. 493892 splicing junctions. 220982 known splicing junctions. 272910 novel splicing junctions.
sampling 50% (175017820) splicing reads. 504089 splicing junctions. 221844 known splicing junctions. 282245 novel splicing junctions.
sampling 55% (192519602) splicing reads. 512785 splicing junctions. 222590 known splicing junctions. 290195 novel splicing junctions.
sampling 60% (210021384) splicing reads. 520283 splicing junctions. 223206 known splicing junctions. 297077 novel splicing junctions.
sampling 65% (227523166) splicing reads. 526768 splicing junctions. 223715 known splicing junctions. 303053 novel splicing junctions.
sampling 70% (245024947) splicing reads. 532537 splicing junctions. 224170 known splicing junctions. 308367 novel splicing junctions.
sampling 75% (262526730) splicing reads. 537409 splicing junctions. 224572 known splicing junctions. 312837 novel splicing junctions.
sampling 80% (280028512) splicing reads. 541701 splicing junctions. 224899 known splicing junctions. 316802 novel splicing junctions.
sampling 85% (297530294) splicing reads. 545249 splicing junctions. 225185 known splicing junctions. 320064 novel splicing junctions.
sampling 90% (315032076) splicing reads. 548176 splicing junctions. 225391 known splicing junctions. 322785 novel splicing junctions.
sampling 95% (332533858) splicing reads. 550772 splicing junctions. 225606 known splicing junctions. 325166 novel splicing junctions.
sampling 100% (350035640) splicing reads. 552917 splicing junctions. 225762 known splicing junctions. 327155 novel splicing junctions.