Done.
-parsing GTF file: /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.gtf
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.iQJiupt9LE/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1_1.fastp.fastq.gz /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
* Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00089_B23WHTKLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index tih_rna_sample_00089_B23WHTKLT4_1.star.sortedByCoord.out.bam
10:11:20 : INFO : Done.
-parsing GTF file: /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.gtf
-parsing /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.star.cSorted.dupsMarked.bam
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[850000] -done parsing /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'low complexity anchor region' => 17,
'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 3,
' ** passed ** ' => 2956,
'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 2,
'per_id < 96' => 3069,
'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 11,
'num_hits: 6 != num_counted_on_fusion_contigs 2 ' => 2,
'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 6,
'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 4,
'excessive soft clipping' => 5020,
'small anchor length' => 5,
'num genes matched < 2' => 9,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 589,
'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 8
};
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
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[102000] -fusion SPANNING read extraction for scaff: ARAP1--FCHSD2
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[25000] -fusion SPANNING read extraction for scaff: AVEN--NFATC2
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[47000] -fusion SPANNING read extraction for scaff: CRYZL2P--SEC16B
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[23000] -fusion SPANNING read extraction for scaff: FAM135A--AL080275.1
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[23000] -fusion SPANNING read extraction for scaff: FAM135A--AL365226.1
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[33000] -fusion SPANNING read extraction for scaff: PRELID3A--AFG3L2
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[38000] -fusion SPANNING read extraction for scaff: PTPRK--RSPO3
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[55000] -fusion SPANNING read extraction for scaff: ROCK1--AFG3L2
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[14000] -fusion SPANNING read extraction for scaff: SAE1--AC011495.1
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[73000] -fusion SPANNING read extraction for scaff: SF3B3--COG4
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[33000] -fusion SPANNING read extraction for scaff: SIN3A--CORO2B
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[10000] -fusion SPANNING read extraction for scaff: SPIRE1--GREB1L
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[25000] -fusion SPANNING read extraction for scaff: TAF4--UBE2Q2
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[10000] -fusion SPANNING read extraction for scaff: TLK2--AC240565.1
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[46000] -fusion SPANNING read extraction for scaff: WSB2--NOS1
-filtered reads reasons: $VAR1 = {
'lacks exon overlap' => 7521
};
EM: Starting log likelihood: -4324.304107
EM: Round [1] log likelihood: -4322.167281
EM: Round [2] log likelihood: -4322.158841
EM: Round [3] log likelihood: -4322.158525
EM: Round [4] log likelihood: -4322.158512
EM: Stopping iterations at round 4 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam into /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam.read_coords
CMD: touch /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam.read_coords > /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam.frag_coords > /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.iQJiupt9LE/fi_workdir/tih_rna_sample_00089_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -4233.664313
EM: Round [1] log likelihood: -4232.019923
EM: Round [2] log likelihood: -4232.012085
EM: Round [3] log likelihood: -4232.011770
EM: Round [4] log likelihood: -4232.011756
EM: Stopping iterations at round 4 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1_1.fastp.fastq.gz: 184812266
Warning - not locating file: /tmp/nxf.iQJiupt9LE/tih_rna_sample_00089_B23WHTKLT4_1.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000242193.11]
WARNING, no entry stored in dbm for [ENSG00000242193.11]
WARNING, no entry stored in dbm for [ENSG00000242193.11]
WARNING, no entry stored in dbm for [ENSG00000280136.2]
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000241818.1]
ls: cannot access 'IGV_inputs': No such file or directory