[W::hts_idx_load3] The index file is older than the data file: 659_cTi-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (17723076) splicing reads. 275976 splicing junctions. 193457 known splicing junctions. 82519 novel splicing junctions.
sampling 10% (35446152) splicing reads. 328756 splicing junctions. 203950 known splicing junctions. 124806 novel splicing junctions.
sampling 15% (53169228) splicing reads. 361056 splicing junctions. 209036 known splicing junctions. 152020 novel splicing junctions.
sampling 20% (70892304) splicing reads. 383660 splicing junctions. 212101 known splicing junctions. 171559 novel splicing junctions.
sampling 25% (88615380) splicing reads. 400344 splicing junctions. 214245 known splicing junctions. 186099 novel splicing junctions.
sampling 30% (106338456) splicing reads. 412955 splicing junctions. 215721 known splicing junctions. 197234 novel splicing junctions.
sampling 35% (124061532) splicing reads. 423268 splicing junctions. 216879 known splicing junctions. 206389 novel splicing junctions.
sampling 40% (141784608) splicing reads. 431550 splicing junctions. 217792 known splicing junctions. 213758 novel splicing junctions.
sampling 45% (159507684) splicing reads. 438191 splicing junctions. 218453 known splicing junctions. 219738 novel splicing junctions.
sampling 50% (177230760) splicing reads. 443798 splicing junctions. 219040 known splicing junctions. 224758 novel splicing junctions.
sampling 55% (194953836) splicing reads. 448554 splicing junctions. 219468 known splicing junctions. 229086 novel splicing junctions.
sampling 60% (212676912) splicing reads. 452505 splicing junctions. 219821 known splicing junctions. 232684 novel splicing junctions.
sampling 65% (230399988) splicing reads. 455885 splicing junctions. 220160 known splicing junctions. 235725 novel splicing junctions.
sampling 70% (248123064) splicing reads. 458646 splicing junctions. 220421 known splicing junctions. 238225 novel splicing junctions.
sampling 75% (265846140) splicing reads. 460995 splicing junctions. 220657 known splicing junctions. 240338 novel splicing junctions.
sampling 80% (283569216) splicing reads. 463028 splicing junctions. 220845 known splicing junctions. 242183 novel splicing junctions.
sampling 85% (301292292) splicing reads. 464741 splicing junctions. 221002 known splicing junctions. 243739 novel splicing junctions.
sampling 90% (319015368) splicing reads. 466131 splicing junctions. 221137 known splicing junctions. 244994 novel splicing junctions.
sampling 95% (336738444) splicing reads. 467238 splicing junctions. 221242 known splicing junctions. 245996 novel splicing junctions.
sampling 100% (354461521) splicing reads. 468054 splicing junctions. 221321 known splicing junctions. 246733 novel splicing junctions.