Done.
-parsing GTF file: /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.gtf
* Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.us79jhaJOe/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1_1.fastp.fastq.gz /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c'
* Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00121_B23WHTKLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index tih_rna_sample_00121_B23WHTKLT4_1.star.sortedByCoord.out.bam
10:58:23 : INFO : Done.
-parsing GTF file: /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.gtf
-parsing /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.star.cSorted.dupsMarked.bam
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[870000] -done parsing /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info.
junction read elimination tally: $VAR1 = {
'seq-similar region overlap' => 137,
'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 42,
'small anchor length' => 56,
' ** passed ** ' => 2716,
'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 140,
'num_hits: 6 != num_counted_on_fusion_contigs 2 ' => 4,
'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 3,
'low complexity anchor region' => 1,
'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 13,
'excessive soft clipping' => 5927,
'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 30,
'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 12,
'per_id < 96' => 3159,
'num genes matched < 2' => 2,
'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 4,
'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 32
};
-writing fusion junction support info.
-outputting the spanning read info: /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
- counting read alignments among fusion contigs.
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[36000] -fusion SPANNING read extraction for scaff: DHX15--PCAT4
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[59000] -fusion SPANNING read extraction for scaff: DHX15--ANTXR2
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[68000] -fusion SPANNING read extraction for scaff: FSIP1--AC013652.1
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[24000] -fusion SPANNING read extraction for scaff: HSPA4L--PWP2
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[83000] -fusion SPANNING read extraction for scaff: LUC7L--PGAP6
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[39000] -fusion SPANNING read extraction for scaff: LUC7L--Z97634.1
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[38000] -fusion SPANNING read extraction for scaff: N4BP2L2--WDR95P
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[81000] -fusion SPANNING read extraction for scaff: PCCA--DOCK9
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[28000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1
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[24000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2
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[28000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13
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[48000] -fusion SPANNING read extraction for scaff: RTN3--ATL3
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[32000] -fusion SPANNING read extraction for scaff: TLK2--FAM157A
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[44000] -fusion SPANNING read extraction for scaff: UBR3--AC078883.1
-filtered reads reasons: $VAR1 = {
'lacks exon overlap' => 36963,
'seq similar region alignment' => 22663
};
EM: Starting log likelihood: -3468.261377
EM: Round [1] log likelihood: -3467.182234
EM: Round [2] log likelihood: -3466.343222
EM: Round [3] log likelihood: -3465.637058
EM: Round [4] log likelihood: -3465.038980
EM: Round [5] log likelihood: -3464.529942
EM: Round [6] log likelihood: -3464.094920
EM: Round [7] log likelihood: -3463.721886
EM: Round [8] log likelihood: -3463.401093
EM: Round [9] log likelihood: -3463.124559
EM: Round [10] log likelihood: -3462.885689
EM: Round [11] log likelihood: -3462.678992
EM: Round [12] log likelihood: -3462.499869
EM: Round [13] log likelihood: -3462.344441
EM: Round [14] log likelihood: -3462.209426
EM: Round [15] log likelihood: -3462.092031
EM: Round [16] log likelihood: -3461.989872
EM: Round [17] log likelihood: -3461.900909
EM: Round [18] log likelihood: -3461.823390
EM: Round [19] log likelihood: -3461.755806
EM: Round [20] log likelihood: -3461.696858
EM: Round [21] log likelihood: -3461.645419
EM: Round [22] log likelihood: -3461.600519
EM: Round [23] log likelihood: -3461.561314
EM: Round [24] log likelihood: -3461.527073
EM: Round [25] log likelihood: -3461.497159
EM: Round [26] log likelihood: -3461.471022
EM: Round [27] log likelihood: -3461.448179
EM: Round [28] log likelihood: -3461.428214
EM: Round [29] log likelihood: -3461.410760
EM: Round [30] log likelihood: -3461.395501
EM: Round [31] log likelihood: -3461.382158
EM: Round [32] log likelihood: -3461.370491
EM: Round [33] log likelihood: -3461.360287
EM: Round [34] log likelihood: -3461.351364
EM: Round [35] log likelihood: -3461.343559
EM: Round [36] log likelihood: -3461.336732
EM: Round [37] log likelihood: -3461.330760
EM: Round [38] log likelihood: -3461.325537
EM: Round [39] log likelihood: -3461.320967
EM: Round [40] log likelihood: -3461.316970
EM: Round [41] log likelihood: -3461.313473
EM: Round [42] log likelihood: -3461.310413
EM: Round [43] log likelihood: -3461.307736
EM: Round [44] log likelihood: -3461.305394
EM: Round [45] log likelihood: -3461.303346
EM: Round [46] log likelihood: -3461.301553
EM: Round [47] log likelihood: -3461.299985
EM: Round [48] log likelihood: -3461.298612
EM: Round [49] log likelihood: -3461.297412
EM: Round [50] log likelihood: -3461.296361
EM: Round [51] log likelihood: -3461.295442
EM: Round [52] log likelihood: -3461.294638
EM: Round [53] log likelihood: -3461.293934
EM: Round [54] log likelihood: -3461.293319
EM: Round [55] log likelihood: -3461.292780
EM: Round [56] log likelihood: -3461.292309
EM: Round [57] log likelihood: -3461.291896
EM: Round [58] log likelihood: -3461.291535
EM: Round [59] log likelihood: -3461.291219
EM: Round [60] log likelihood: -3461.290943
EM: Round [61] log likelihood: -3461.290701
EM: Round [62] log likelihood: -3461.290490
EM: Round [63] log likelihood: -3461.290305
EM: Round [64] log likelihood: -3461.290143
EM: Round [65] log likelihood: -3461.290001
EM: Round [66] log likelihood: -3461.289877
EM: Round [67] log likelihood: -3461.289769
EM: Round [68] log likelihood: -3461.289674
EM: Stopping iterations at round 68 due to insufficient improvement in likelihood.
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
-extracting read coordinates from /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam into /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords
CMD: touch /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords > /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.frag_coords > /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.frag_coords.coord_sorted
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: cp /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.post_blast_and_promiscuity_filter
-done, see /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
EM: Starting log likelihood: -3381.944027
EM: Round [1] log likelihood: -3381.918992
EM: Round [2] log likelihood: -3381.918789
EM: Round [3] log likelihood: -3381.918787
EM: Stopping iterations at round 3 due to insufficient improvement in likelihood.
-total frags in /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1_1.fastp.fastq.gz: 295776955
Warning - not locating file: /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.gmap_trinity_GG.fusions.gff3.bed
WARNING, no entry stored in dbm for [ENSG00000251321.1]
WARNING, no entry stored in dbm for [ENSG00000251321.1]
WARNING, no entry stored in dbm for [ENSG00000236438.7]
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
WARNING, no entry stored in dbm for [ENSG00000225205.5]
ls: cannot access 'IGV_inputs': No such file or directory