Done. -parsing GTF file: /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.gtf * Running CMD: /usr/local/bin/STAR --runThreadN 8 --genomeDir /tmp/nxf.us79jhaJOe/ctat_genome_lib_build_dir/ref_genome.fa.star.idx --outSAMtype BAM SortedByCoordinate --twopassMode None --alignSJDBoverhangMin 10 --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705 --alignInsertionFlush Right --alignMatesGapMax 100000 --alignIntronMax 100000 --outSAMattributes All --readFilesIn /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1_1.fastp.fastq.gz /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1_2.fastp.fastq.gz --genomeFastaFiles /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fa --outSAMfilter KeepAllAddedReferences --sjdbGTFfile /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.gtf --alignSJstitchMismatchNmax 5 -1 5 5 --scoreGapNoncan -6 --readFilesCommand 'gunzip -c' * Running CMD: mv Aligned.sortedByCoord.out.bam tih_rna_sample_00121_B23WHTKLT4_1.star.sortedByCoord.out.bam * Running CMD: samtools index tih_rna_sample_00121_B23WHTKLT4_1.star.sortedByCoord.out.bam 10:58:23 : INFO : Done. -parsing GTF file: /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.gtf -parsing /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.star.cSorted.dupsMarked.bam [10000] [20000] [30000] [40000] [50000] [60000] [70000] [80000] [90000] [100000] [110000] [120000] [130000] [140000] [150000] [160000] [170000] [180000] [190000] [200000] [210000] [220000] [230000] [240000] [250000] [260000] [270000] [280000] [290000] [300000] [310000] [320000] [330000] [340000] [350000] [360000] [370000] [380000] [390000] [400000] [410000] [420000] [430000] [440000] [450000] [460000] [470000] [480000] [490000] [500000] [510000] [520000] [530000] [540000] [550000] [560000] [570000] [580000] [590000] [600000] [610000] [620000] [630000] [640000] [650000] [660000] [670000] [680000] [690000] [700000] [710000] [720000] [730000] [740000] [750000] [760000] [770000] [780000] [790000] [800000] [810000] [820000] [830000] [840000] [850000] [860000] [870000] -done parsing /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.star.cSorted.dupsMarked.bam. Extracting junction info. junction read elimination tally: $VAR1 = { 'seq-similar region overlap' => 137, 'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 42, 'small anchor length' => 56, ' ** passed ** ' => 2716, 'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 140, 'num_hits: 6 != num_counted_on_fusion_contigs 2 ' => 4, 'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 3, 'low complexity anchor region' => 1, 'num_hits: 4 != num_counted_on_fusion_contigs 1 ' => 13, 'excessive soft clipping' => 5927, 'num_hits: 5 != num_counted_on_fusion_contigs 2 ' => 30, 'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 12, 'per_id < 96' => 3159, 'num genes matched < 2' => 2, 'num_hits: 5 != num_counted_on_fusion_contigs 4 ' => 4, 'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 32 }; -writing fusion junction support info. -outputting the spanning read info: /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info. - counting read alignments among fusion contigs. [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] -fusion SPANNING read extraction for scaff: CWC27--SREK1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] -fusion SPANNING read extraction for scaff: DHX15--PCAT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] -fusion SPANNING read extraction for scaff: DHX15--ANTXR2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] -fusion SPANNING read extraction for scaff: DOCK9--PCCA [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] -fusion SPANNING read extraction for scaff: FSIP1--AC013652.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] -fusion SPANNING read extraction for scaff: HSPA4L--PWP2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] -fusion SPANNING read extraction for scaff: INTS6--CDK8 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] [82000] [83000] -fusion SPANNING read extraction for scaff: LUC7L--PGAP6 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] -fusion SPANNING read extraction for scaff: LUC7L--Z97634.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] -fusion SPANNING read extraction for scaff: N4BP2L2--WDR95P [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] [49000] [50000] [51000] [52000] [53000] [54000] [55000] [56000] [57000] [58000] [59000] [60000] [61000] [62000] [63000] [64000] [65000] [66000] [67000] [68000] [69000] [70000] [71000] [72000] [73000] [74000] [75000] [76000] [77000] [78000] [79000] [80000] [81000] -fusion SPANNING read extraction for scaff: PCCA--DOCK9 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] [45000] [46000] [47000] [48000] -fusion SPANNING read extraction for scaff: RTN3--ATL3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] -fusion SPANNING read extraction for scaff: TLK2--FAM157A [1000] [2000] [3000] [4000] [5000] -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] -fusion SPANNING read extraction for scaff: TMBIM4--MSRB3 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] -fusion SPANNING read extraction for scaff: TVP23C--CDRT4 [1000] [2000] [3000] [4000] [5000] [6000] [7000] [8000] [9000] [10000] [11000] [12000] [13000] [14000] [15000] [16000] [17000] [18000] [19000] [20000] [21000] [22000] [23000] [24000] [25000] [26000] [27000] [28000] [29000] [30000] [31000] [32000] [33000] [34000] [35000] [36000] [37000] [38000] [39000] [40000] [41000] [42000] [43000] [44000] -fusion SPANNING read extraction for scaff: UBR3--AC078883.1 -filtered reads reasons: $VAR1 = { 'lacks exon overlap' => 36963, 'seq similar region alignment' => 22663 }; EM: Starting log likelihood: -3468.261377 EM: Round [1] log likelihood: -3467.182234 EM: Round [2] log likelihood: -3466.343222 EM: Round [3] log likelihood: -3465.637058 EM: Round [4] log likelihood: -3465.038980 EM: Round [5] log likelihood: -3464.529942 EM: Round [6] log likelihood: -3464.094920 EM: Round [7] log likelihood: -3463.721886 EM: Round [8] log likelihood: -3463.401093 EM: Round [9] log likelihood: -3463.124559 EM: Round [10] log likelihood: -3462.885689 EM: Round [11] log likelihood: -3462.678992 EM: Round [12] log likelihood: -3462.499869 EM: Round [13] log likelihood: -3462.344441 EM: Round [14] log likelihood: -3462.209426 EM: Round [15] log likelihood: -3462.092031 EM: Round [16] log likelihood: -3461.989872 EM: Round [17] log likelihood: -3461.900909 EM: Round [18] log likelihood: -3461.823390 EM: Round [19] log likelihood: -3461.755806 EM: Round [20] log likelihood: -3461.696858 EM: Round [21] log likelihood: -3461.645419 EM: Round [22] log likelihood: -3461.600519 EM: Round [23] log likelihood: -3461.561314 EM: Round [24] log likelihood: -3461.527073 EM: Round [25] log likelihood: -3461.497159 EM: Round [26] log likelihood: -3461.471022 EM: Round [27] log likelihood: -3461.448179 EM: Round [28] log likelihood: -3461.428214 EM: Round [29] log likelihood: -3461.410760 EM: Round [30] log likelihood: -3461.395501 EM: Round [31] log likelihood: -3461.382158 EM: Round [32] log likelihood: -3461.370491 EM: Round [33] log likelihood: -3461.360287 EM: Round [34] log likelihood: -3461.351364 EM: Round [35] log likelihood: -3461.343559 EM: Round [36] log likelihood: -3461.336732 EM: Round [37] log likelihood: -3461.330760 EM: Round [38] log likelihood: -3461.325537 EM: Round [39] log likelihood: -3461.320967 EM: Round [40] log likelihood: -3461.316970 EM: Round [41] log likelihood: -3461.313473 EM: Round [42] log likelihood: -3461.310413 EM: Round [43] log likelihood: -3461.307736 EM: Round [44] log likelihood: -3461.305394 EM: Round [45] log likelihood: -3461.303346 EM: Round [46] log likelihood: -3461.301553 EM: Round [47] log likelihood: -3461.299985 EM: Round [48] log likelihood: -3461.298612 EM: Round [49] log likelihood: -3461.297412 EM: Round [50] log likelihood: -3461.296361 EM: Round [51] log likelihood: -3461.295442 EM: Round [52] log likelihood: -3461.294638 EM: Round [53] log likelihood: -3461.293934 EM: Round [54] log likelihood: -3461.293319 EM: Round [55] log likelihood: -3461.292780 EM: Round [56] log likelihood: -3461.292309 EM: Round [57] log likelihood: -3461.291896 EM: Round [58] log likelihood: -3461.291535 EM: Round [59] log likelihood: -3461.291219 EM: Round [60] log likelihood: -3461.290943 EM: Round [61] log likelihood: -3461.290701 EM: Round [62] log likelihood: -3461.290490 EM: Round [63] log likelihood: -3461.290305 EM: Round [64] log likelihood: -3461.290143 EM: Round [65] log likelihood: -3461.290001 EM: Round [66] log likelihood: -3461.289877 EM: Round [67] log likelihood: -3461.289769 EM: Round [68] log likelihood: -3461.289674 EM: Stopping iterations at round 68 due to insufficient improvement in likelihood. [bam_sort_core] merging from 0 files and 8 in-memory blocks... -extracting read coordinates from /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam into /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords CMD: touch /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords.ok CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords > /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.read_coords.sort_by_readname CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.frag_coords > /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.consolidated.bam.frag_coords.coord_sorted CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50 CMD: cp /tmp/nxf.us79jhaJOe/fi_workdir/tih_rna_sample_00121_B23WHTKLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.post_blast_and_promiscuity_filter -done, see /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass EM: Starting log likelihood: -3381.944027 EM: Round [1] log likelihood: -3381.918992 EM: Round [2] log likelihood: -3381.918789 EM: Round [3] log likelihood: -3381.918787 EM: Stopping iterations at round 3 due to insufficient improvement in likelihood. -total frags in /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1_1.fastp.fastq.gz: 295776955 Warning - not locating file: /tmp/nxf.us79jhaJOe/tih_rna_sample_00121_B23WHTKLT4_1.gmap_trinity_GG.fusions.gff3.bed WARNING, no entry stored in dbm for [ENSG00000251321.1] WARNING, no entry stored in dbm for [ENSG00000251321.1] WARNING, no entry stored in dbm for [ENSG00000236438.7] WARNING, no entry stored in dbm for [ENSG00000226049.3] WARNING, no entry stored in dbm for [ENSG00000260404.3] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000282057.1] WARNING, no entry stored in dbm for [ENSG00000259345.7] WARNING, no entry stored in dbm for [ENSG00000259345.7] WARNING, no entry stored in dbm for [ENSG00000225205.5] ls: cannot access 'IGV_inputs': No such file or directory