Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/74/672104bfbbc6f98f5f891326b816b6/.command.sh Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/a80b510e6d9a6da7b5a5074b7bef61/fi_workdir/659_cfy-T1-TRNA-1_B23WHTKLT4_1.gtf Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/74/672104bfbbc6f98f5f891326b816b6/.command.run ==> STAGING COMPLETE (3 inputs) Using standard /usr/local/lib/perl5/site_perl/auto/share/dist/AGAT/agat_config.yaml file ------------------------------------------------------------------------------ | Another GFF Analysis Toolkit (AGAT) - Version: v1.2.0 | | https://github.com/NBISweden/AGAT | | National Bioinformatics Infrastructure Sweden (NBIS) - www.nbis.se | ------------------------------------------------------------------------------ ------ Start parsing ------ -------------------------- parse options and metadata -------------------------- => Accessing the feature_levels YAML file Using standard /usr/local/lib/perl5/site_perl/auto/share/dist/AGAT/feature_levels.yaml file => Attribute used to group features when no Parent/ID relationship exists (i.e common tag): * locus_tag * gene_id => merge_loci option deactivated => Machine information: This script is being run by perl v5.32.1 Bioperl location being used: /usr/local/lib/perl5/site_perl/Bio/ Operating system being used: linux => Accessing Ontology No ontology accessible from the gff file header! We use the SOFA ontology distributed with AGAT: /usr/local/lib/perl5/site_perl/auto/share/dist/AGAT/so.obo Read ontology /usr/local/lib/perl5/site_perl/auto/share/dist/AGAT/so.obo: 4 root terms, and 2596 total terms, and 1516 leaf terms Filtering ontology: We found 1861 terms that are sequence_feature or is_a child of it. --------------------------------- parsing file --------------------------------- => Number of line in file: 2097 => Number of comment lines: 0 => Fasta included: No => Number of features lines: 2097 => Number of feature type (3rd column): 2 * Level1: 0 => * level2: 0 => * level3: 2 => exon CDS * unknown: 0 => =>Check because only level3 features: * Number of feature with Parent attribute:0 * Number of feature with a common attribute:2097 => Some common attributes and some Parent attributes missing. /!\ For features where both are missing A single Level2 features (e.g. mRNA) and a single level1 (e.g. gene) will be created by AGAT, and all level3 feautres (e,g, CDS,exon) will be attached to them. This is probably not what you want... see B. 2.2 and 3. at https://agat.readthedocs.io/en/latest/agat_how_does_it_work.html /!\ For features where the common attribute or the parent attribute is missing, it would be fine as long as you do not expect isoforms in your annotation (Eukaryote). see B. 4. at https://agat.readthedocs.io/en/latest/agat_how_does_it_work.html !! You might try to fix the issue by choosing a common tag attribute to use in order to group the features correctly (parameter --ct in agat_convert_sp_gxf2gxf.pl). => Version of the Bioperl GFF parser selected by AGAT: 2 ------ End parsing (done in 1 second) ------ ------ Start checks ------ ---------------------------- Check1: feature types ----------------------------- ----------------------------------- ontology ----------------------------------- All feature types in agreement with the Ontology. ------------------------------------- agat ------------------------------------- AGAT can deal with all the encountered feature types (3rd column) ------------------------------ done in 0 seconds ------------------------------- ------------------------------ Check2: duplicates ------------------------------ None found ------------------------------ done in 0 seconds ------------------------------- -------------------------- Check3: sequential bucket --------------------------- Nothing to check as sequential bucket! ------------------------------ done in 0 seconds ------------------------------- --------------------------- Check4: l2 linked to l3 ---------------------------- L1 and L2 created: ACTBP6--ACTB HAVANA gene 6203 10583 . + . FI_gene_label "ACTB^ENSG00000075624.17" ; ID "ACTBP6--ACTB^ACTB^ENSG00000075624.17" ; exon_id "ENSE00003542066.1" ; exon_number 3 ; gene_id "ACTBP6--ACTB^ACTB^ENSG00000075624.17" ; gene_name ACTB ; gene_type protein_coding ; havana_gene "OTTHUMG00000023268.12" ; havana_transcript "OTTHUMT00000324028.2" ; hgnc_id "HGNC:132" ; level 2 ; orig_coord_info "chr7,5529535,5529657,-" ; protein_id "ENSP00000401032.1" ; tag alternative_5_UTR mRNA_end_NF cds_end_NF dotter_confirmed ; transcript_id "ACTBP6--ACTB^ENST00000414620.1" ; transcript_name "ACTB-201" ; transcript_support_level 4 ; transcript_type protein_coding ACTBP6--ACTB HAVANA mRNA 6203 10583 . + . FI_gene_label "ACTB^ENSG00000075624.17" ; ID "ACTBP6--ACTB^ENST00000414620.1" ; Parent "ACTBP6--ACTB^ACTB^ENSG00000075624.17" ; exon_id "ENSE00003542066.1" ; exon_number 3 ; gene_id "ACTBP6--ACTB^ACTB^ENSG00000075624.17" ; gene_name ACTB ; gene_type protein_coding ; havana_gene "OTTHUMG00000023268.12" ; havana_transcript "OTTHUMT00000324028.2" ; hgnc_id "HGNC:132" ; level 2 ; orig_coord_info "chr7,5529535,5529657,-" ; protein_id "ENSP00000401032.1" ; tag alternative_5_UTR mRNA_end_NF cds_end_NF dotter_confirmed ; transcript_id "ACTBP6--ACTB^ENST00000414620.1" ; transcript_name "ACTB-201" ; transcript_support_level 4 ; transcript_type protein_coding L1 and L2 created: BCAR3--GLMN HAVANA gene 11472 31098 . + . FI_gene_label "BCAR3^ENSG00000137936.18" ; ID "BCAR3--GLMN^BCAR3^ENSG00000137936.18" ; ccdsid "CCDS745.1" ; exon_id "ENSE00001452146.1" ; exon_number 2 ; gene_id "BCAR3--GLMN^BCAR3^ENSG00000137936.18" ; gene_name BCAR3 ; gene_type protein_coding ; havana_gene "OTTHUMG00000010301.5" ; havana_transcript "OTTHUMT00000028418.2" ; hgnc_id "HGNC:973" ; level 2 ; orig_coord_info "chr1,93674614,93674930,-" ; protein_id "ENSP00000260502.6" ; tag basic MANE_Select appris_principal_4 CCDS ; transcript_id "BCAR3--GLMN^ENST00000260502.11" ; transcript_name "BCAR3-201" ; transcript_support_level 1 ; transcript_type protein_coding BCAR3--GLMN HAVANA mRNA 11472 31098 . + . FI_gene_label "BCAR3^ENSG00000137936.18" ; ID "BCAR3--GLMN^ENST00000260502.11" ; Parent "BCAR3--GLMN^BCAR3^ENSG00000137936.18" ; ccdsid "CCDS745.1" ; exon_id "ENSE00001452146.1" ; exon_number 2 ; gene_id "BCAR3--GLMN^BCAR3^ENSG00000137936.18" ; gene_name BCAR3 ; gene_type protein_coding ; havana_gene "OTTHUMG00000010301.5" ; havana_transcript "OTTHUMT00000028418.2" ; hgnc_id "HGNC:973" ; level 2 ; orig_coord_info "chr1,93674614,93674930,-" ; protein_id "ENSP00000260502.6" ; tag basic MANE_Select appris_principal_4 CCDS ; transcript_id "BCAR3--GLMN^ENST00000260502.11" ; transcript_name "BCAR3-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: BCAR3--GLMN HAVANA gene 34099 50823 . + . FI_gene_label "GLMN^ENSG00000174842.17" ; ID "BCAR3--GLMN^GLMN^ENSG00000174842.17" ; ccdsid "CCDS738.1" ; exon_id "ENSE00002159430.1" ; exon_number 2 ; gene_id "BCAR3--GLMN^GLMN^ENSG00000174842.17" ; gene_name GLMN ; gene_type protein_coding ; havana_gene "OTTHUMG00000010283.9" ; havana_transcript "OTTHUMT00000028358.2" ; hgnc_id "HGNC:14373" ; level 2 ; orig_coord_info "chr1,92297961,92297999,-" ; protein_id "ENSP00000359385.3" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "BCAR3--GLMN^ENST00000370360.8" ; transcript_name "GLMN-201" ; transcript_support_level 1 ; transcript_type protein_coding BCAR3--GLMN HAVANA mRNA 34099 50823 . + . FI_gene_label "GLMN^ENSG00000174842.17" ; ID "BCAR3--GLMN^ENST00000370360.8" ; Parent "BCAR3--GLMN^GLMN^ENSG00000174842.17" ; ccdsid "CCDS738.1" ; exon_id "ENSE00002159430.1" ; exon_number 2 ; gene_id "BCAR3--GLMN^GLMN^ENSG00000174842.17" ; gene_name GLMN ; gene_type protein_coding ; havana_gene "OTTHUMG00000010283.9" ; havana_transcript "OTTHUMT00000028358.2" ; hgnc_id "HGNC:14373" ; level 2 ; orig_coord_info "chr1,92297961,92297999,-" ; protein_id "ENSP00000359385.3" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "BCAR3--GLMN^ENST00000370360.8" ; transcript_name "GLMN-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: DYRK1A--LINC02756 HAVANA gene 2985 36922 . + . FI_gene_label "DYRK1A^ENSG00000157540.22" ; ID "DYRK1A--LINC02756^DYRK1A^ENSG00000157540.22" ; ccdsid "CCDS42926.1" ; exon_id "ENSE00003829613.1" ; exon_number 3 ; gene_id "DYRK1A--LINC02756^DYRK1A^ENSG00000157540.22" ; gene_name DYRK1A ; gene_type protein_coding ; havana_gene "OTTHUMG00000086657.9" ; havana_transcript "OTTHUMT00000194799.2" ; hgnc_id "HGNC:3091" ; level 2 ; orig_coord_info "chr21,37420375,37420384,+" ; protein_id "ENSP00000342690.3" ; tag basic CCDS ; transcript_id "DYRK1A--LINC02756^ENST00000338785.8" ; transcript_name "DYRK1A-201" ; transcript_support_level 1 ; transcript_type protein_coding DYRK1A--LINC02756 HAVANA mRNA 2985 36922 . + . FI_gene_label "DYRK1A^ENSG00000157540.22" ; ID "DYRK1A--LINC02756^ENST00000338785.8" ; Parent "DYRK1A--LINC02756^DYRK1A^ENSG00000157540.22" ; ccdsid "CCDS42926.1" ; exon_id "ENSE00003829613.1" ; exon_number 3 ; gene_id "DYRK1A--LINC02756^DYRK1A^ENSG00000157540.22" ; gene_name DYRK1A ; gene_type protein_coding ; havana_gene "OTTHUMG00000086657.9" ; havana_transcript "OTTHUMT00000194799.2" ; hgnc_id "HGNC:3091" ; level 2 ; orig_coord_info "chr21,37420375,37420384,+" ; protein_id "ENSP00000342690.3" ; tag basic CCDS ; transcript_id "DYRK1A--LINC02756^ENST00000338785.8" ; transcript_name "DYRK1A-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: FGFR3--TACC3 HAVANA gene 1020 10219 . + . FI_gene_label "FGFR3^ENSG00000068078.20" ; ID "FGFR3--TACC3^FGFR3^ENSG00000068078.20" ; exon_id "ENSE00001596390.1" ; exon_number 2 ; gene_id "FGFR3--TACC3^FGFR3^ENSG00000068078.20" ; gene_name FGFR3 ; gene_type protein_coding ; havana_gene "OTTHUMG00000121148.7" ; havana_transcript "OTTHUMT00000495785.1" ; hgnc_id "HGNC:3690" ; level 2 ; orig_coord_info "chr4,1793935,1794043,+" ; protein_id "ENSP00000260795.3" ; transcript_id "FGFR3--TACC3^ENST00000260795.8" ; transcript_name "FGFR3-201" ; transcript_support_level 1 ; transcript_type nonsense_mediated_decay FGFR3--TACC3 HAVANA mRNA 1020 10219 . + . FI_gene_label "FGFR3^ENSG00000068078.20" ; ID "FGFR3--TACC3^ENST00000260795.8" ; Parent "FGFR3--TACC3^FGFR3^ENSG00000068078.20" ; exon_id "ENSE00001596390.1" ; exon_number 2 ; gene_id "FGFR3--TACC3^FGFR3^ENSG00000068078.20" ; gene_name FGFR3 ; gene_type protein_coding ; havana_gene "OTTHUMG00000121148.7" ; havana_transcript "OTTHUMT00000495785.1" ; hgnc_id "HGNC:3690" ; level 2 ; orig_coord_info "chr4,1793935,1794043,+" ; protein_id "ENSP00000260795.3" ; transcript_id "FGFR3--TACC3^ENST00000260795.8" ; transcript_name "FGFR3-201" ; transcript_support_level 1 ; transcript_type nonsense_mediated_decay L1 and L2 created: FGFR3--TACC3 HAVANA gene 16535 31742 . + . FI_gene_label "TACC3^ENSG00000013810.20" ; ID "FGFR3--TACC3^TACC3^ENSG00000013810.20" ; ccdsid "CCDS3352.1" ; exon_id "ENSE00003597099.1" ; exon_number 2 ; gene_id "FGFR3--TACC3^TACC3^ENSG00000013810.20" ; gene_name TACC3 ; gene_type protein_coding ; havana_gene "OTTHUMG00000089535.25" ; havana_transcript "OTTHUMT00000203730.4" ; hgnc_id "HGNC:11524" ; level 2 ; orig_coord_info "chr4,1723422,1723583,+" ; protein_id "ENSP00000326550.4" ; tag CAGE_supported_TSS basic MANE_Select appris_principal_2 CCDS ; transcript_id "FGFR3--TACC3^ENST00000313288.9" ; transcript_name "TACC3-201" ; transcript_support_level 1 ; transcript_type protein_coding FGFR3--TACC3 HAVANA mRNA 16535 31742 . + . FI_gene_label "TACC3^ENSG00000013810.20" ; ID "FGFR3--TACC3^ENST00000313288.9" ; Parent "FGFR3--TACC3^TACC3^ENSG00000013810.20" ; ccdsid "CCDS3352.1" ; exon_id "ENSE00003597099.1" ; exon_number 2 ; gene_id "FGFR3--TACC3^TACC3^ENSG00000013810.20" ; gene_name TACC3 ; gene_type protein_coding ; havana_gene "OTTHUMG00000089535.25" ; havana_transcript "OTTHUMT00000203730.4" ; hgnc_id "HGNC:11524" ; level 2 ; orig_coord_info "chr4,1723422,1723583,+" ; protein_id "ENSP00000326550.4" ; tag CAGE_supported_TSS basic MANE_Select appris_principal_2 CCDS ; transcript_id "FGFR3--TACC3^ENST00000313288.9" ; transcript_name "TACC3-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: MTAP--DMRTA1 HAVANA gene 29696 36281 . + . FI_gene_label "DMRTA1^ENSG00000176399.4" ; ID "MTAP--DMRTA1^DMRTA1^ENSG00000176399.4" ; ccdsid "CCDS6514.1" ; exon_id "ENSE00001268100.3" ; exon_number 1 ; gene_id "MTAP--DMRTA1^DMRTA1^ENSG00000176399.4" ; gene_name DMRTA1 ; gene_type protein_coding ; havana_gene "OTTHUMG00000019693.3" ; havana_transcript "OTTHUMT00000051935.3" ; hgnc_id "HGNC:13826" ; level 2 ; orig_coord_info "chr9,22447066,22447732,+" ; protein_id "ENSP00000319651.1" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "MTAP--DMRTA1^ENST00000325870.3" ; transcript_name "DMRTA1-201" ; transcript_support_level 1 ; transcript_type protein_coding MTAP--DMRTA1 HAVANA mRNA 29696 36281 . + . FI_gene_label "DMRTA1^ENSG00000176399.4" ; ID "MTAP--DMRTA1^ENST00000325870.3" ; Parent "MTAP--DMRTA1^DMRTA1^ENSG00000176399.4" ; ccdsid "CCDS6514.1" ; exon_id "ENSE00001268100.3" ; exon_number 1 ; gene_id "MTAP--DMRTA1^DMRTA1^ENSG00000176399.4" ; gene_name DMRTA1 ; gene_type protein_coding ; havana_gene "OTTHUMG00000019693.3" ; havana_transcript "OTTHUMT00000051935.3" ; hgnc_id "HGNC:13826" ; level 2 ; orig_coord_info "chr9,22447066,22447732,+" ; protein_id "ENSP00000319651.1" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "MTAP--DMRTA1^ENST00000325870.3" ; transcript_name "DMRTA1-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: MTAP--DMRTA1 HAVANA gene 1022 14638 . + . FI_gene_label "MTAP^ENSG00000099810.21" ; ID "MTAP--DMRTA1^MTAP^ENSG00000099810.21" ; exon_id "ENSE00001547793.2" ; exon_number 1 ; gene_id "MTAP--DMRTA1^MTAP^ENSG00000099810.21" ; gene_name MTAP ; gene_type protein_coding ; havana_gene "OTTHUMG00000019690.10" ; havana_transcript "OTTHUMT00000334287.1" ; hgnc_id "HGNC:7413" ; level 2 ; orig_coord_info "chr9,21802749,21802781,+" ; protein_id "ENSP00000393507.1" ; tag not_organism_supported ; transcript_id "MTAP--DMRTA1^ENST00000419385.5" ; transcript_name "MTAP-201" ; transcript_support_level 5 ; transcript_type nonsense_mediated_decay MTAP--DMRTA1 HAVANA mRNA 1022 14638 . + . FI_gene_label "MTAP^ENSG00000099810.21" ; ID "MTAP--DMRTA1^ENST00000419385.5" ; Parent "MTAP--DMRTA1^MTAP^ENSG00000099810.21" ; exon_id "ENSE00001547793.2" ; exon_number 1 ; gene_id "MTAP--DMRTA1^MTAP^ENSG00000099810.21" ; gene_name MTAP ; gene_type protein_coding ; havana_gene "OTTHUMG00000019690.10" ; havana_transcript "OTTHUMT00000334287.1" ; hgnc_id "HGNC:7413" ; level 2 ; orig_coord_info "chr9,21802749,21802781,+" ; protein_id "ENSP00000393507.1" ; tag not_organism_supported ; transcript_id "MTAP--DMRTA1^ENST00000419385.5" ; transcript_name "MTAP-201" ; transcript_support_level 5 ; transcript_type nonsense_mediated_decay L1 and L2 created: MTAP--LINC01239 HAVANA gene 1022 14638 . + . FI_gene_label "MTAP^ENSG00000099810.21" ; ID "MTAP--LINC01239^MTAP^ENSG00000099810.21" ; exon_id "ENSE00001547793.2" ; exon_number 1 ; gene_id "MTAP--LINC01239^MTAP^ENSG00000099810.21" ; gene_name MTAP ; gene_type protein_coding ; havana_gene "OTTHUMG00000019690.10" ; havana_transcript "OTTHUMT00000334287.1" ; hgnc_id "HGNC:7413" ; level 2 ; orig_coord_info "chr9,21802749,21802781,+" ; protein_id "ENSP00000393507.1" ; tag not_organism_supported ; transcript_id "MTAP--LINC01239^ENST00000419385.5" ; transcript_name "MTAP-201" ; transcript_support_level 5 ; transcript_type nonsense_mediated_decay MTAP--LINC01239 HAVANA mRNA 1022 14638 . + . FI_gene_label "MTAP^ENSG00000099810.21" ; ID "MTAP--LINC01239^ENST00000419385.5" ; Parent "MTAP--LINC01239^MTAP^ENSG00000099810.21" ; exon_id "ENSE00001547793.2" ; exon_number 1 ; gene_id "MTAP--LINC01239^MTAP^ENSG00000099810.21" ; gene_name MTAP ; gene_type protein_coding ; havana_gene "OTTHUMG00000019690.10" ; havana_transcript "OTTHUMT00000334287.1" ; hgnc_id "HGNC:7413" ; level 2 ; orig_coord_info "chr9,21802749,21802781,+" ; protein_id "ENSP00000393507.1" ; tag not_organism_supported ; transcript_id "MTAP--LINC01239^ENST00000419385.5" ; transcript_name "MTAP-201" ; transcript_support_level 5 ; transcript_type nonsense_mediated_decay L1 and L2 created: NUS1--AC114501.3 HAVANA gene 1001 9659 . + . FI_gene_label "NUS1^ENSG00000153989.8" ; ID "NUS1--AC114501.3^NUS1^ENSG00000153989.8" ; ccdsid "CCDS5118.1" ; exon_id "ENSE00001447272.4" ; exon_number 1 ; gene_id "NUS1--AC114501.3^NUS1^ENSG00000153989.8" ; gene_name NUS1 ; gene_type protein_coding ; havana_gene "OTTHUMG00000015458.2" ; havana_transcript "OTTHUMT00000041989.2" ; hgnc_id "HGNC:21042" ; level 2 ; orig_coord_info "chr6,117675671,117676085,+" ; protein_id "ENSP00000357480.3" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "NUS1--AC114501.3^ENST00000368494.4" ; transcript_name "NUS1-201" ; transcript_support_level 1 ; transcript_type protein_coding NUS1--AC114501.3 HAVANA mRNA 1001 9659 . + . FI_gene_label "NUS1^ENSG00000153989.8" ; ID "NUS1--AC114501.3^ENST00000368494.4" ; Parent "NUS1--AC114501.3^NUS1^ENSG00000153989.8" ; ccdsid "CCDS5118.1" ; exon_id "ENSE00001447272.4" ; exon_number 1 ; gene_id "NUS1--AC114501.3^NUS1^ENSG00000153989.8" ; gene_name NUS1 ; gene_type protein_coding ; havana_gene "OTTHUMG00000015458.2" ; havana_transcript "OTTHUMT00000041989.2" ; hgnc_id "HGNC:21042" ; level 2 ; orig_coord_info "chr6,117675671,117676085,+" ; protein_id "ENSP00000357480.3" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "NUS1--AC114501.3^ENST00000368494.4" ; transcript_name "NUS1-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: TVP23C--CDRT4 HAVANA gene 1052 15760 . + . FI_gene_label "TVP23C^ENSG00000175106.17" ; ID "TVP23C--CDRT4^TVP23C^ENSG00000175106.17" ; ccdsid "CCDS11170.1" ; exon_id "ENSE00001105678.2" ; exon_number 1 ; gene_id "TVP23C--CDRT4^TVP23C^ENSG00000175106.17" ; gene_name TVP23C ; gene_type protein_coding ; havana_gene "OTTHUMG00000171461.10" ; havana_transcript "OTTHUMT00000130705.3" ; hgnc_id "HGNC:30453" ; level 2 ; orig_coord_info "chr17,15563437,15563448,-" ; protein_id "ENSP00000225576.3" ; tag basic CCDS ; transcript_id "TVP23C--CDRT4^ENST00000225576.7" ; transcript_name "TVP23C-201" ; transcript_support_level 1 ; transcript_type protein_coding TVP23C--CDRT4 HAVANA mRNA 1052 15760 . + . FI_gene_label "TVP23C^ENSG00000175106.17" ; ID "TVP23C--CDRT4^ENST00000225576.7" ; Parent "TVP23C--CDRT4^TVP23C^ENSG00000175106.17" ; ccdsid "CCDS11170.1" ; exon_id "ENSE00001105678.2" ; exon_number 1 ; gene_id "TVP23C--CDRT4^TVP23C^ENSG00000175106.17" ; gene_name TVP23C ; gene_type protein_coding ; havana_gene "OTTHUMG00000171461.10" ; havana_transcript "OTTHUMT00000130705.3" ; hgnc_id "HGNC:30453" ; level 2 ; orig_coord_info "chr17,15563437,15563448,-" ; protein_id "ENSP00000225576.3" ; tag basic CCDS ; transcript_id "TVP23C--CDRT4^ENST00000225576.7" ; transcript_name "TVP23C-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: TVP23C--CDRT4 HAVANA gene 19889 25887 . + . FI_gene_label "CDRT4^ENSG00000239704.11" ; ID "TVP23C--CDRT4^CDRT4^ENSG00000239704.11" ; ccdsid "CCDS73995.1" ; exon_id "ENSE00003735347.1" ; exon_number 3 ; gene_id "TVP23C--CDRT4^CDRT4^ENSG00000239704.11" ; gene_name CDRT4 ; gene_type protein_coding ; havana_gene "OTTHUMG00000059070.14" ; havana_transcript "OTTHUMT00000130383.9" ; hgnc_id "HGNC:14383" ; level 2 ; orig_coord_info "chr17,15440208,15440238,-" ; protein_id "ENSP00000482523.1" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "TVP23C--CDRT4^ENST00000619038.5" ; transcript_name "CDRT4-204" ; transcript_support_level 1 ; transcript_type protein_coding TVP23C--CDRT4 HAVANA mRNA 19889 25887 . + . FI_gene_label "CDRT4^ENSG00000239704.11" ; ID "TVP23C--CDRT4^ENST00000619038.5" ; Parent "TVP23C--CDRT4^CDRT4^ENSG00000239704.11" ; ccdsid "CCDS73995.1" ; exon_id "ENSE00003735347.1" ; exon_number 3 ; gene_id "TVP23C--CDRT4^CDRT4^ENSG00000239704.11" ; gene_name CDRT4 ; gene_type protein_coding ; havana_gene "OTTHUMG00000059070.14" ; havana_transcript "OTTHUMT00000130383.9" ; hgnc_id "HGNC:14383" ; level 2 ; orig_coord_info "chr17,15440208,15440238,-" ; protein_id "ENSP00000482523.1" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "TVP23C--CDRT4^ENST00000619038.5" ; transcript_name "CDRT4-204" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: UBR5--BAALC-AS1 HAVANA gene 1071 53320 . + . FI_gene_label "UBR5^ENSG00000104517.13" ; ID "UBR5--BAALC-AS1^UBR5^ENSG00000104517.13" ; ccdsid "CCDS64946.1" ; exon_id "ENSE00001162016.4" ; exon_number 1 ; gene_id "UBR5--BAALC-AS1^UBR5^ENSG00000104517.13" ; gene_name UBR5 ; gene_type protein_coding ; havana_gene "OTTHUMG00000164755.5" ; havana_transcript "OTTHUMT00000380196.1" ; hgnc_id "HGNC:16806" ; level 2 ; orig_coord_info "chr8,102412173,102412234,-" ; protein_id "ENSP00000220959.4" ; tag basic appris_alternative_1 CCDS ; transcript_id "UBR5--BAALC-AS1^ENST00000220959.8" ; transcript_name "UBR5-201" ; transcript_support_level 1 ; transcript_type protein_coding UBR5--BAALC-AS1 HAVANA mRNA 1071 53320 . + . FI_gene_label "UBR5^ENSG00000104517.13" ; ID "UBR5--BAALC-AS1^ENST00000220959.8" ; Parent "UBR5--BAALC-AS1^UBR5^ENSG00000104517.13" ; ccdsid "CCDS64946.1" ; exon_id "ENSE00001162016.4" ; exon_number 1 ; gene_id "UBR5--BAALC-AS1^UBR5^ENSG00000104517.13" ; gene_name UBR5 ; gene_type protein_coding ; havana_gene "OTTHUMG00000164755.5" ; havana_transcript "OTTHUMT00000380196.1" ; hgnc_id "HGNC:16806" ; level 2 ; orig_coord_info "chr8,102412173,102412234,-" ; protein_id "ENSP00000220959.4" ; tag basic appris_alternative_1 CCDS ; transcript_id "UBR5--BAALC-AS1^ENST00000220959.8" ; transcript_name "UBR5-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: ZNF431--RPSAP58 HAVANA gene 1017 22132 . + . FI_gene_label "ZNF431^ENSG00000196705.8" ; ID "ZNF431--RPSAP58^ZNF431^ENSG00000196705.8" ; ccdsid "CCDS32979.1" ; exon_id "ENSE00003141770.1" ; exon_number 1 ; gene_id "ZNF431--RPSAP58^ZNF431^ENSG00000196705.8" ; gene_name ZNF431 ; gene_type protein_coding ; havana_gene "OTTHUMG00000182835.3" ; havana_transcript "OTTHUMT00000463943.2" ; hgnc_id "HGNC:20809" ; level 2 ; orig_coord_info "chr19,21142184,21142186,+" ; protein_id "ENSP00000308578.6" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "ZNF431--RPSAP58^ENST00000311048.11" ; transcript_name "ZNF431-201" ; transcript_support_level 1 ; transcript_type protein_coding ZNF431--RPSAP58 HAVANA mRNA 1017 22132 . + . FI_gene_label "ZNF431^ENSG00000196705.8" ; ID "ZNF431--RPSAP58^ENST00000311048.11" ; Parent "ZNF431--RPSAP58^ZNF431^ENSG00000196705.8" ; ccdsid "CCDS32979.1" ; exon_id "ENSE00003141770.1" ; exon_number 1 ; gene_id "ZNF431--RPSAP58^ZNF431^ENSG00000196705.8" ; gene_name ZNF431 ; gene_type protein_coding ; havana_gene "OTTHUMG00000182835.3" ; havana_transcript "OTTHUMT00000463943.2" ; hgnc_id "HGNC:20809" ; level 2 ; orig_coord_info "chr19,21142184,21142186,+" ; protein_id "ENSP00000308578.6" ; tag basic MANE_Select appris_principal_1 CCDS ; transcript_id "ZNF431--RPSAP58^ENST00000311048.11" ; transcript_name "ZNF431-201" ; transcript_support_level 1 ; transcript_type protein_coding L1 and L2 created: ACTBP6--ACTB HAVANA gene 1001 2090 . + . FI_gene_label "ACTBP6^ENSG00000203413.3" ; ID "ACTBP6--ACTB^ACTBP6^ENSG00000203413.3" ; exon_id "ENSE00002116857.1" ; exon_number 1 ; gene_id "ACTBP6--ACTB^ACTBP6^ENSG00000203413.3" ; gene_name ACTBP6 ; gene_type processed_pseudogene ; havana_gene "OTTHUMG00000164632.2" ; havana_transcript "OTTHUMT00000379462.2" ; hgnc_id "HGNC:139" ; level 1 ; ont "PGO:0000004" ; orig_coord_info "chr8,84948585,84949674,+" ; tag pseudo_consens basic ; transcript_id "ACTBP6--ACTB^ENST00000520036.1" ; transcript_name "ACTBP6-201" ; transcript_support_level NA ; transcript_type processed_pseudogene ACTBP6--ACTB HAVANA RNA 1001 2090 . + . FI_gene_label "ACTBP6^ENSG00000203413.3" ; ID "ACTBP6--ACTB^ENST00000520036.1" ; Parent "ACTBP6--ACTB^ACTBP6^ENSG00000203413.3" ; exon_id "ENSE00002116857.1" ; exon_number 1 ; gene_id "ACTBP6--ACTB^ACTBP6^ENSG00000203413.3" ; gene_name ACTBP6 ; gene_type processed_pseudogene ; havana_gene "OTTHUMG00000164632.2" ; havana_transcript "OTTHUMT00000379462.2" ; hgnc_id "HGNC:139" ; level 1 ; ont "PGO:0000004" ; orig_coord_info "chr8,84948585,84949674,+" ; tag pseudo_consens basic ; transcript_id "ACTBP6--ACTB^ENST00000520036.1" ; transcript_name "ACTBP6-201" ; transcript_support_level NA ; transcript_type processed_pseudogene L1 and L2 created: DYRK1A--LINC02756 HAVANA gene 50943 56986 . + . FI_gene_label "LINC02756^ENSG00000255332.8" ; ID "DYRK1A--LINC02756^LINC02756^ENSG00000255332.8" ; exon_id "ENSE00002711875.2" ; exon_number 1 ; gene_id "DYRK1A--LINC02756^LINC02756^ENSG00000255332.8" ; gene_name LINC02756 ; gene_type lncRNA ; havana_gene "OTTHUMG00000167330.10" ; havana_transcript "OTTHUMT00000447347.2" ; hgnc_id "HGNC:54276" ; level 1 ; orig_coord_info "chr11,91794358,91794422,+" ; tag basic TAGENE exp_conf ; transcript_id "DYRK1A--LINC02756^ENST00000525832.3" ; transcript_name "LINC02756-201" ; transcript_support_level 3 ; transcript_type lncRNA DYRK1A--LINC02756 HAVANA RNA 50943 56986 . + . FI_gene_label "LINC02756^ENSG00000255332.8" ; ID "DYRK1A--LINC02756^ENST00000525832.3" ; Parent "DYRK1A--LINC02756^LINC02756^ENSG00000255332.8" ; exon_id "ENSE00002711875.2" ; exon_number 1 ; gene_id "DYRK1A--LINC02756^LINC02756^ENSG00000255332.8" ; gene_name LINC02756 ; gene_type lncRNA ; havana_gene "OTTHUMG00000167330.10" ; havana_transcript "OTTHUMT00000447347.2" ; hgnc_id "HGNC:54276" ; level 1 ; orig_coord_info "chr11,91794358,91794422,+" ; tag basic TAGENE exp_conf ; transcript_id "DYRK1A--LINC02756^ENST00000525832.3" ; transcript_name "LINC02756-201" ; transcript_support_level 3 ; transcript_type lncRNA L1 and L2 created: MTAP--LINC01239 HAVANA gene 32024 33348 . + . FI_gene_label "LINC01239^ENSG00000234840.2" ; ID "MTAP--LINC01239^LINC01239^ENSG00000234840.2" ; exon_id "ENSE00001647776.1" ; exon_number 1 ; gene_id "MTAP--LINC01239^LINC01239^ENSG00000234840.2" ; gene_name LINC01239 ; gene_type lncRNA ; havana_gene "OTTHUMG00000019696.2" ; havana_transcript "OTTHUMT00000051939.1" ; hgnc_id "HGNC:49796" ; level 2 ; orig_coord_info "chr9,22682470,22682713,+" ; transcript_id "MTAP--LINC01239^ENST00000433645.1" ; transcript_name "LINC01239-201" ; transcript_support_level 3 ; transcript_type retained_intron MTAP--LINC01239 HAVANA RNA 32024 33348 . + . FI_gene_label "LINC01239^ENSG00000234840.2" ; ID "MTAP--LINC01239^ENST00000433645.1" ; Parent "MTAP--LINC01239^LINC01239^ENSG00000234840.2" ; exon_id "ENSE00001647776.1" ; exon_number 1 ; gene_id "MTAP--LINC01239^LINC01239^ENSG00000234840.2" ; gene_name LINC01239 ; gene_type lncRNA ; havana_gene "OTTHUMG00000019696.2" ; havana_transcript "OTTHUMT00000051939.1" ; hgnc_id "HGNC:49796" ; level 2 ; orig_coord_info "chr9,22682470,22682713,+" ; transcript_id "MTAP--LINC01239^ENST00000433645.1" ; transcript_name "LINC01239-201" ; transcript_support_level 3 ; transcript_type retained_intron L1 and L2 created: NUS1--AC114501.3 HAVANA gene 12660 13027 . + . FI_gene_label "AC114501.3^ENSG00000238124.1" ; ID "NUS1--AC114501.3^AC114501.3^ENSG00000238124.1" ; exon_id "ENSE00001603093.1" ; exon_number 1 ; gene_id "NUS1--AC114501.3^AC114501.3^ENSG00000238124.1" ; gene_name "AC114501.3" ; gene_type lncRNA ; havana_gene "OTTHUMG00000156731.1" ; havana_transcript "OTTHUMT00000345494.1" ; level 2 ; orig_coord_info "chr7,65463071,65463168,+" ; tag basic ; transcript_id "NUS1--AC114501.3^ENST00000453648.1" ; transcript_name "AC114501.3-201" ; transcript_support_level 5 ; transcript_type lncRNA NUS1--AC114501.3 HAVANA RNA 12660 13027 . + . FI_gene_label "AC114501.3^ENSG00000238124.1" ; ID "NUS1--AC114501.3^ENST00000453648.1" ; Parent "NUS1--AC114501.3^AC114501.3^ENSG00000238124.1" ; exon_id "ENSE00001603093.1" ; exon_number 1 ; gene_id "NUS1--AC114501.3^AC114501.3^ENSG00000238124.1" ; gene_name "AC114501.3" ; gene_type lncRNA ; havana_gene "OTTHUMG00000156731.1" ; havana_transcript "OTTHUMT00000345494.1" ; level 2 ; orig_coord_info "chr7,65463071,65463168,+" ; tag basic ; transcript_id "NUS1--AC114501.3^ENST00000453648.1" ; transcript_name "AC114501.3-201" ; transcript_support_level 5 ; transcript_type lncRNA L1 and L2 created: UBR5--BAALC-AS1 HAVANA gene 59208 75845 . + . FI_gene_label "BAALC-AS1^ENSG00000247081.8" ; ID "UBR5--BAALC-AS1^BAALC-AS1^ENSG00000247081.8" ; exon_id "ENSE00001974416.1" ; exon_number 1 ; gene_id "UBR5--BAALC-AS1^BAALC-AS1^ENSG00000247081.8" ; gene_name "BAALC-AS1" ; gene_type lncRNA ; havana_gene "OTTHUMG00000164798.6" ; havana_transcript "OTTHUMT00000380348.1" ; hgnc_id "HGNC:50461" ; level 2 ; orig_coord_info "chr8,103285838,103285907,-" ; tag basic ; transcript_id "UBR5--BAALC-AS1^ENST00000499522.6" ; transcript_name "BAALC-AS1-201" ; transcript_support_level 5 ; transcript_type lncRNA UBR5--BAALC-AS1 HAVANA RNA 59208 75845 . + . FI_gene_label "BAALC-AS1^ENSG00000247081.8" ; ID "UBR5--BAALC-AS1^ENST00000499522.6" ; Parent "UBR5--BAALC-AS1^BAALC-AS1^ENSG00000247081.8" ; exon_id "ENSE00001974416.1" ; exon_number 1 ; gene_id "UBR5--BAALC-AS1^BAALC-AS1^ENSG00000247081.8" ; gene_name "BAALC-AS1" ; gene_type lncRNA ; havana_gene "OTTHUMG00000164798.6" ; havana_transcript "OTTHUMT00000380348.1" ; hgnc_id "HGNC:50461" ; level 2 ; orig_coord_info "chr8,103285838,103285907,-" ; tag basic ; transcript_id "UBR5--BAALC-AS1^ENST00000499522.6" ; transcript_name "BAALC-AS1-201" ; transcript_support_level 5 ; transcript_type lncRNA L1 and L2 created: ZNF431--RPSAP58 HAVANA gene 25133 26020 . + . FI_gene_label "RPSAP58^ENSG00000225178.5" ; ID "ZNF431--RPSAP58^RPSAP58^ENSG00000225178.5" ; exon_id "ENSE00001619993.3" ; exon_number 1 ; gene_id "ZNF431--RPSAP58^RPSAP58^ENSG00000225178.5" ; gene_name RPSAP58 ; gene_type processed_pseudogene ; havana_gene "OTTHUMG00000158122.3" ; havana_transcript "OTTHUMT00000350238.3" ; hgnc_id "HGNC:36809" ; level 1 ; ont "PGO:0000004" ; orig_coord_info "chr19,23827162,23828049,+" ; tag pseudo_consens basic ; transcript_id "ZNF431--RPSAP58^ENST00000484897.3" ; transcript_name "RPSAP58-201" ; transcript_support_level NA ; transcript_type processed_pseudogene ZNF431--RPSAP58 HAVANA RNA 25133 26020 . + . FI_gene_label "RPSAP58^ENSG00000225178.5" ; ID "ZNF431--RPSAP58^ENST00000484897.3" ; Parent "ZNF431--RPSAP58^RPSAP58^ENSG00000225178.5" ; exon_id "ENSE00001619993.3" ; exon_number 1 ; gene_id "ZNF431--RPSAP58^RPSAP58^ENSG00000225178.5" ; gene_name RPSAP58 ; gene_type processed_pseudogene ; havana_gene "OTTHUMG00000158122.3" ; havana_transcript "OTTHUMT00000350238.3" ; hgnc_id "HGNC:36809" ; level 1 ; ont "PGO:0000004" ; orig_coord_info "chr19,23827162,23828049,+" ; tag pseudo_consens basic ; transcript_id "ZNF431--RPSAP58^ENST00000484897.3" ; transcript_name "RPSAP58-201" ; transcript_support_level NA ; transcript_type processed_pseudogene 180 cases fixed where L3 features have parent feature(s) missing ------------------------------ done in 0 seconds ------------------------------- --------------------------- Check5: l1 linked to l2 ---------------------------- No problem found ------------------------------ done in 0 seconds ------------------------------- --------------------------- Check6: remove orphan l1 --------------------------- We remove only those not supposed to be orphan None found ------------------------------ done in 0 seconds ------------------------------- ------------------------- Check7: all level3 locations ------------------------- ------------------------------ done in 0 seconds ------------------------------- ------------------------------ Check8: check cds ------------------------------- No problem found ------------------------------ done in 0 seconds ------------------------------- ----------------------------- Check9: check exons ------------------------------ No exons created No exons locations modified No supernumerary exons removed No level2 locations modified ------------------------------ done in 0 seconds ------------------------------- ----------------------------- Check10: check utrs ------------------------------ 281 UTRs created that were missing No UTRs locations modified No supernumerary UTRs removed ------------------------------ done in 0 seconds ------------------------------- ------------------------ Check11: all level2 locations ------------------------- No problem found ------------------------------ done in 0 seconds ------------------------------- ------------------------ Check12: all level1 locations ------------------------- We fixed 14 wrong level1 location cases ------------------------------ done in 0 seconds ------------------------------- ---------------------- Check13: remove identical isoforms ---------------------- None found ------------------------------ done in 0 seconds ------------------------------- ------ End checks (done in 0 second) ------ GFF3 file parsed