Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/852da4d144028843eda989ae64b42c/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa Downloading: s3://natera-platform-sandbox/pipeline-resources/Homo_sapiens_assembly38.dbsnp138.vcf.gz Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/db/26745f87158335bc91069c9744a37a/.command.sh Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.dict Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/gatk4/dbsnp_hg38_contam_sites.bed Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/db/26745f87158335bc91069c9744a37a/.command.run Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/ref_genome.fa.fai Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/4d/852da4d144028843eda989ae64b42c/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam Downloading: s3://natera-platform-sandbox/pipeline-resources/beds/twist/Twist_rna_exome_AND_fusion_hg38.bed Downloading: s3://natera-platform-sandbox/pipeline-resources/Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi ==> STAGING COMPLETE (11 inputs) + mkdir 659_bCu-T1-TRNA-1_B23WHTKLT4_1 + all_qc -bam 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder 659_bCu-T1-TRNA-1_B23WHTKLT4_1 -reference ref_genome.fa -region chr20 mkdir -p 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/ cat Twist_rna_exome_AND_fusion_hg38.bed | cut -f 1-3 | bedtools sort -g ref_genome.fa.fai | bedtools merge > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/full_bed.bed seqtool depth -bam 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam -bed 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/full_bed.bed -threads 4 -bam_threads 2 | bgzip -c > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth.gz saturation_plot -bams 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam -region chr20 -output 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/saturation_plot.png -threads 1 seqtool saturation_metric -at 1000000 -region chr20 -bam_threads 1 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/saturation_qc.tsv fragment_size -bams 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam -region chr20 -output_prefix 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/fragsize -threads 1 samtools mpileup -B --min-MQ 60 --min-BQ 30 -f ref_genome.fa -l dbsnp_hg38_contam_sites.bed 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam > 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.mpileup && contamination -input 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.mpileup -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/contamination.tsv [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai 0it [00:00, ?it/s][W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai 106683it [00:00, 1066726.74it/s] 227063it [00:00, 1147295.97it/s] 343717it [00:00, 1155836.65it/s] 463006it [00:00, 1170427.76it/s] 583782it [00:00, 1183792.15it/s] 703378it [00:00, 1187892.47it/s] 826711it [00:00, 1202709.25it/s] 946983it [00:00, 1200803.79it/s] 1067359it [00:00, 1201646.72it/s] 1188931it [00:01, 1205839.13it/s] 1309516it [00:01, 1203553.09it/s] 1430861it [00:01, 1206522.98it/s] 1551515it [00:01, 1197755.56it/s] 1671305it [00:01, 1190644.11it/s] 1794621it [00:01, 1203238.18it/s] 1914967it [00:01, 1196560.44it/s][W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai 2036132it [00:01, 1200948.04it/s] 2156244it [00:01, 1185089.68it/s] 2274804it [00:01, 1171956.01it/s] 2392053it [00:02, 1156605.27it/s] 2507772it [00:02, 1156700.40it/s][W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai 2629003it [00:02, 1173109.13it/s] 2746368it [00:02, 1172236.13it/s] 2866337it [00:02, 1180369.29it/s] 2984407it [00:02, 1178888.50it/s] 3104657it [00:02, 1185783.67it/s] 3223479it [00:02, 1186478.54it/s] 3280276it [00:02, 1184609.46it/s] [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai tabix -s 1 -b 2 -e 2 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth.gz seqtool depth_hist -nbins 500 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth.gz > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth_hist.tsv cat 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth_hist.tsv | plot -x 0 -xlabel Depth -ylabel "Number of Sites" -xlim 0 2000 -title 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md -output 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth.png [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi seqtool read_qc -bed Twist_rna_exome_AND_fusion_hg38.bed -n_threads 1 -bam_threads 2 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/read_qc.Twist_rna_exome_AND_fusion_hg38.bed.tsv seqtool reads_per_target -bed Twist_rna_exome_AND_fusion_hg38.bed -threads 1 -bam_threads 2 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/reads_per_target.Twist_rna_exome_AND_fusion_hg38.bed.tsv seqtool depth_stats -bed Twist_rna_exome_AND_fusion_hg38.bed 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.depth.gz> 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/depth_stats.Twist_rna_exome_AND_fusion_hg38.bed.tsv seqtool gc_bias -reference ref_genome.fa -bed Twist_rna_exome_AND_fusion_hg38.bed -bin_size 0.05 -threads 1 -bam_threads 2 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bam > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/gc_bias_qc.Twist_rna_exome_AND_fusion_hg38.bed.tsv [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: 659_bCu-T1-TRNA-1_B23WHTKLT4_1.md.bai cat 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/gc_bias_qc.Twist_rna_exome_AND_fusion_hg38.bed.tsv | plot -x 0 -header infer -ylim 0 2 -title "659_bCu-T1-TRNA-1_B23WHTKLT4_1.md" -suptitle "Twist_rna_exome_AND_fusion_hg38.bed" -output "659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/gc_bias.Twist_rna_exome_AND_fusion_hg38.bed.png" -ylabel "Fold enrichment / depletion" -xlabel "GC Quantile" echo `date` > 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md/qc_complete.txt + merge_qc -folders 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1.md -output 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1_full_basic_stats.tsv + head -n1 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed 659_bCu-T1-TRNA-1_B23WHTKLT4_1/659_bCu-T1-TRNA-1_B23WHTKLT4_1_full_basic_stats.tsv