[W::hts_idx_load3] The index file is older than the data file: 659_c95-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (5846872) splicing reads. 204932 splicing junctions. 166993 known splicing junctions. 37939 novel splicing junctions.
sampling 10% (11693745) splicing reads. 244062 splicing junctions. 180596 known splicing junctions. 63466 novel splicing junctions.
sampling 15% (17540617) splicing reads. 271605 splicing junctions. 188063 known splicing junctions. 83542 novel splicing junctions.
sampling 20% (23387490) splicing reads. 293159 splicing junctions. 193003 known splicing junctions. 100156 novel splicing junctions.
sampling 25% (29234362) splicing reads. 311034 splicing junctions. 196799 known splicing junctions. 114235 novel splicing junctions.
sampling 30% (35081235) splicing reads. 326091 splicing junctions. 199660 known splicing junctions. 126431 novel splicing junctions.
sampling 35% (40928107) splicing reads. 339028 splicing junctions. 201910 known splicing junctions. 137118 novel splicing junctions.
sampling 40% (46774980) splicing reads. 350334 splicing junctions. 203780 known splicing junctions. 146554 novel splicing junctions.
sampling 45% (52621852) splicing reads. 360324 splicing junctions. 205377 known splicing junctions. 154947 novel splicing junctions.
sampling 50% (58468725) splicing reads. 369029 splicing junctions. 206668 known splicing junctions. 162361 novel splicing junctions.
sampling 55% (64315598) splicing reads. 376727 splicing junctions. 207792 known splicing junctions. 168935 novel splicing junctions.
sampling 60% (70162470) splicing reads. 383557 splicing junctions. 208762 known splicing junctions. 174795 novel splicing junctions.
sampling 65% (76009343) splicing reads. 389720 splicing junctions. 209641 known splicing junctions. 180079 novel splicing junctions.
sampling 70% (81856215) splicing reads. 395235 splicing junctions. 210394 known splicing junctions. 184841 novel splicing junctions.
sampling 75% (87703088) splicing reads. 399855 splicing junctions. 211007 known splicing junctions. 188848 novel splicing junctions.
sampling 80% (93549960) splicing reads. 404030 splicing junctions. 211565 known splicing junctions. 192465 novel splicing junctions.
sampling 85% (99396833) splicing reads. 407623 splicing junctions. 212042 known splicing junctions. 195581 novel splicing junctions.
sampling 90% (105243705) splicing reads. 410638 splicing junctions. 212407 known splicing junctions. 198231 novel splicing junctions.
sampling 95% (111090578) splicing reads. 413238 splicing junctions. 212714 known splicing junctions. 200524 novel splicing junctions.
sampling 100% (116937451) splicing reads. 415320 splicing junctions. 212976 known splicing junctions. 202344 novel splicing junctions.