[W::hts_idx_load3] The index file is older than the data file: 659_buD-T1-TRNA-1_B23WHTKLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (5686843) splicing reads. 206164 splicing junctions. 168428 known splicing junctions. 37736 novel splicing junctions.
sampling 10% (11373686) splicing reads. 244232 splicing junctions. 181234 known splicing junctions. 62998 novel splicing junctions.
sampling 15% (17060529) splicing reads. 270814 splicing junctions. 188286 known splicing junctions. 82528 novel splicing junctions.
sampling 20% (22747373) splicing reads. 291173 splicing junctions. 192972 known splicing junctions. 98201 novel splicing junctions.
sampling 25% (28434216) splicing reads. 307607 splicing junctions. 196320 known splicing junctions. 111287 novel splicing junctions.
sampling 30% (34121059) splicing reads. 321164 splicing junctions. 198804 known splicing junctions. 122360 novel splicing junctions.
sampling 35% (39807902) splicing reads. 332657 splicing junctions. 200815 known splicing junctions. 131842 novel splicing junctions.
sampling 40% (45494746) splicing reads. 342468 splicing junctions. 202401 known splicing junctions. 140067 novel splicing junctions.
sampling 45% (51181589) splicing reads. 350865 splicing junctions. 203739 known splicing junctions. 147126 novel splicing junctions.
sampling 50% (56868432) splicing reads. 358247 splicing junctions. 204857 known splicing junctions. 153390 novel splicing junctions.
sampling 55% (62555275) splicing reads. 364678 splicing junctions. 205852 known splicing junctions. 158826 novel splicing junctions.
sampling 60% (68242119) splicing reads. 370278 splicing junctions. 206629 known splicing junctions. 163649 novel splicing junctions.
sampling 65% (73928962) splicing reads. 375200 splicing junctions. 207274 known splicing junctions. 167926 novel splicing junctions.
sampling 70% (79615805) splicing reads. 379404 splicing junctions. 207833 known splicing junctions. 171571 novel splicing junctions.
sampling 75% (85302648) splicing reads. 383079 splicing junctions. 208340 known splicing junctions. 174739 novel splicing junctions.
sampling 80% (90989492) splicing reads. 386143 splicing junctions. 208755 known splicing junctions. 177388 novel splicing junctions.
sampling 85% (96676335) splicing reads. 388810 splicing junctions. 209087 known splicing junctions. 179723 novel splicing junctions.
sampling 90% (102363178) splicing reads. 390987 splicing junctions. 209398 known splicing junctions. 181589 novel splicing junctions.
sampling 95% (108050021) splicing reads. 392829 splicing junctions. 209649 known splicing junctions. 183180 novel splicing junctions.
sampling 100% (113736865) splicing reads. 394256 splicing junctions. 209843 known splicing junctions. 184413 novel splicing junctions.