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BCL CONVERT QC REPORT - DETAILED
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1. % UNDETERMINED PER LANE
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%_Undetermined Undetermined_Reads Total_Reads
Lane
1 7.42 293146516 3949083474
2 7.56 298323753 3944247355
3 7.52 300084169 3991640170
4 8.11 320493726 3953564323
5 7.80 306473228 3928620214
6 7.38 291458533 3951964639
7 7.40 293572378 3969347905
8 7.47 295033479 3951217964
2. SAMPLES WITH LOW READS (NON-NTC)
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No samples found with low reads
3. NTC SAMPLES WITH HIGH READS
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No NTC samples found with high reads
4. TOP UNKNOWN BARCODES
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Found 118 significant unknown barcodes:
Lane index index2 # Reads % of Unknown Barcodes % of All Reads Percentage_of_Lane Percentage_of_Total
0 1 GAGCAGCTTG GGGGGGGGGG 6537395 0.022301 0.001655 0.165542 0.020662
1 1 AGATTAGCGT GGGGGGGGGG 5535460 0.018883 0.001402 0.140171 0.017495
2 1 TATCCGCGTC GGGGGGGGGG 4892126 0.016688 0.001239 0.123880 0.015462
3 1 ATGACAGCAC GGGGGGGGGG 4182645 0.014268 0.001059 0.105914 0.013220
4 1 CCTAAGCTGA GGGGGGGGGG 4111586 0.014026 0.001041 0.104115 0.012995
5 1 TGGAGATCGA GGGGGGGGGG 3446135 0.011756 0.000873 0.087264 0.010892
6 1 ACGAGACGTC GGGGGGGGGG 3156661 0.010768 0.000799 0.079934 0.009977
7 1 CCAGTCGTCA GGGGGGGGGG 2937326 0.010020 0.000744 0.074380 0.009284
8 1 TCCAACTGAA GGGGGGGGGG 2769644 0.009448 0.000701 0.070134 0.008754
9 1 TAACTTGGTC GGGGGGGGGG 2761038 0.009419 0.000699 0.069916 0.008727
10 1 CAACTGCGCA GGGGGGGGGG 2748459 0.009376 0.000696 0.069597 0.008687
11 1 GCCACCTAGT GGGGGGGGGG 2495508 0.008513 0.000632 0.063192 0.007887
12 1 CCGATAGTAG GGGGGGGGGG 2366714 0.008073 0.000599 0.059931 0.007480
13 1 AGAAGACCTA GGGGGGGGGG 2119381 0.007230 0.000537 0.053668 0.006698
14 2 GAGCAGCTTG GGGGGGGGGG 6641120 0.022261 0.001684 0.168375 0.020990
15 2 AGATTAGCGT GGGGGGGGGG 5449301 0.018266 0.001382 0.138158 0.017223
16 2 TATCCGCGTC GGGGGGGGGG 4955465 0.016611 0.001256 0.125638 0.015662
17 2 ATGACAGCAC GGGGGGGGGG 4211400 0.014117 0.001068 0.106773 0.013310
18 2 CCTAAGCTGA GGGGGGGGGG 4087443 0.013701 0.001036 0.103630 0.012919
19 2 TGGAGATCGA GGGGGGGGGG 3537594 0.011858 0.000897 0.089690 0.011181
20 2 ACGAGACGTC GGGGGGGGGG 3187855 0.010686 0.000808 0.080823 0.010075
21 2 CCAGTCGTCA GGGGGGGGGG 2890989 0.009691 0.000733 0.073296 0.009137
22 2 CAACTGCGCA GGGGGGGGGG 2856930 0.009577 0.000724 0.072433 0.009030
23 2 TCCAACTGAA GGGGGGGGGG 2799089 0.009383 0.000710 0.070966 0.008847
24 2 TAACTTGGTC GGGGGGGGGG 2785889 0.009338 0.000706 0.070632 0.008805
25 2 GCCACCTAGT GGGGGGGGGG 2638597 0.008845 0.000669 0.066897 0.008340
26 2 CCGATAGTAG GGGGGGGGGG 2461817 0.008252 0.000624 0.062415 0.007781
27 2 AGAAGACCTA GGGGGGGGGG 2230135 0.007476 0.000565 0.056541 0.007049
28 3 GAGCAGCTTG GGGGGGGGGG 6378005 0.021254 0.001598 0.159784 0.020158
29 3 AGATTAGCGT GGGGGGGGGG 5382754 0.017937 0.001349 0.134851 0.017013
30 3 TATCCGCGTC GGGGGGGGGG 4776580 0.015917 0.001197 0.119665 0.015097
31 3 ATGACAGCAC GGGGGGGGGG 4057071 0.013520 0.001016 0.101639 0.012823
32 3 CCTAAGCTGA GGGGGGGGGG 3980784 0.013266 0.000997 0.099728 0.012582
33 3 TGGAGATCGA GGGGGGGGGG 3375513 0.011249 0.000846 0.084565 0.010669
34 3 ACGAGACGTC GGGGGGGGGG 3141988 0.010470 0.000787 0.078714 0.009931
35 3 CCAGTCGTCA GGGGGGGGGG 2833223 0.009441 0.000710 0.070979 0.008955
36 3 CAACTGCGCA GGGGGGGGGG 2722088 0.009071 0.000682 0.068195 0.008603
37 3 TCCAACTGAA GGGGGGGGGG 2686627 0.008953 0.000673 0.067306 0.008491
38 3 TAACTTGGTC GGGGGGGGGG 2665861 0.008884 0.000668 0.066786 0.008426
39 3 GCCACCTAGT GGGGGGGGGG 2574254 0.008578 0.000645 0.064491 0.008136
40 3 CCGATAGTAG GGGGGGGGGG 2499674 0.008330 0.000626 0.062623 0.007900
41 3 AGAAGACCTA GGGGGGGGGG 2211202 0.007369 0.000554 0.055396 0.006989
42 4 GAGCAGCTTG GGGGGGGGGG 6227501 0.019431 0.001575 0.157516 0.019683
43 4 AGATTAGCGT GGGGGGGGGG 5292554 0.016514 0.001339 0.133868 0.016728
44 4 TATCCGCGTC GGGGGGGGGG 4778675 0.014910 0.001209 0.120870 0.015103
45 4 ATGACAGCAC GGGGGGGGGG 4008656 0.012508 0.001014 0.101393 0.012670
46 4 CCTAAGCTGA GGGGGGGGGG 3808092 0.011882 0.000963 0.096320 0.012036
47 4 TGGAGATCGA GGGGGGGGGG 3434181 0.010715 0.000869 0.086863 0.010854
48 4 ACGAGACGTC GGGGGGGGGG 3124039 0.009748 0.000790 0.079018 0.009874
49 4 CCAGTCGTCA GGGGGGGGGG 2820524 0.008801 0.000713 0.071341 0.008915
50 4 CAACTGCGCA GGGGGGGGGG 2796171 0.008725 0.000707 0.070725 0.008838
51 4 GCCACCTAGT GGGGGGGGGG 2783808 0.008686 0.000704 0.070413 0.008798
52 4 TCCAACTGAA GGGGGGGGGG 2718936 0.008484 0.000688 0.068772 0.008593
53 4 TAACTTGGTC GGGGGGGGGG 2611712 0.008149 0.000661 0.066060 0.008255
54 4 CCGATAGTAG GGGGGGGGGG 2570494 0.008020 0.000650 0.065017 0.008124
55 4 AGAAGACCTA GGGGGGGGGG 2517039 0.007854 0.000637 0.063665 0.007955
56 4 CGATGTCTTG GGGGGGGGGG 2218670 0.006923 0.000561 0.056118 0.007012
57 4 ATGTTGCCAC GGGGGGGGGG 2152189 0.006715 0.000544 0.054437 0.006802
58 4 TCGACGGCAA GGGGGGGGGG 2085748 0.006508 0.000528 0.052756 0.006592
59 5 GAGCAGCTTG GGGGGGGGGG 6242873 0.020370 0.001589 0.158908 0.019731
60 5 AGATTAGCGT GGGGGGGGGG 5214857 0.017016 0.001327 0.132740 0.016482
61 5 TATCCGCGTC GGGGGGGGGG 4728492 0.015429 0.001204 0.120360 0.014945
62 5 ATGACAGCAC GGGGGGGGGG 3979820 0.012986 0.001013 0.101303 0.012579
63 5 CCTAAGCTGA GGGGGGGGGG 3839783 0.012529 0.000977 0.097739 0.012136
64 5 TGGAGATCGA GGGGGGGGGG 3376821 0.011018 0.000860 0.085954 0.010673
65 5 ACGAGACGTC GGGGGGGGGG 3082398 0.010058 0.000785 0.078460 0.009742
66 5 CCAGTCGTCA GGGGGGGGGG 2780589 0.009073 0.000708 0.070778 0.008788
67 5 CAACTGCGCA GGGGGGGGGG 2741802 0.008946 0.000698 0.069790 0.008666
68 5 GCCACCTAGT GGGGGGGGGG 2689826 0.008777 0.000685 0.068467 0.008501
69 5 TCCAACTGAA GGGGGGGGGG 2683410 0.008756 0.000683 0.068304 0.008481
70 5 TAACTTGGTC GGGGGGGGGG 2610255 0.008517 0.000664 0.066442 0.008250
71 5 CCGATAGTAG GGGGGGGGGG 2474120 0.008073 0.000630 0.062977 0.007820
72 5 AGAAGACCTA GGGGGGGGGG 2383841 0.007778 0.000607 0.060679 0.007534
73 5 ATGTTGCCAC GGGGGGGGGG 2055462 0.006707 0.000523 0.052320 0.006496
74 5 CGATGTCTTG GGGGGGGGGG 2011793 0.006564 0.000512 0.051209 0.006358
75 5 TCGACGGCAA GGGGGGGGGG 1964893 0.006411 0.000500 0.050015 0.006210
76 6 GAGCAGCTTG GGGGGGGGGG 6449352 0.022128 0.001632 0.163194 0.020384
77 6 AGATTAGCGT GGGGGGGGGG 5364438 0.018405 0.001357 0.135741 0.016955
78 6 TATCCGCGTC GGGGGGGGGG 4815778 0.016523 0.001219 0.121858 0.015221
79 6 ATGACAGCAC GGGGGGGGGG 4094210 0.014047 0.001036 0.103599 0.012940
80 6 CCTAAGCTGA GGGGGGGGGG 4022061 0.013800 0.001018 0.101774 0.012712
81 6 TGGAGATCGA GGGGGGGGGG 3361130 0.011532 0.000850 0.085050 0.010623
82 6 ACGAGACGTC GGGGGGGGGG 3133017 0.010749 0.000793 0.079277 0.009902
83 6 CCAGTCGTCA GGGGGGGGGG 2865502 0.009832 0.000725 0.072508 0.009057
84 6 TAACTTGGTC GGGGGGGGGG 2705186 0.009282 0.000685 0.068452 0.008550
85 6 CAACTGCGCA GGGGGGGGGG 2698089 0.009257 0.000683 0.068272 0.008528
86 6 TCCAACTGAA GGGGGGGGGG 2697029 0.009254 0.000682 0.068245 0.008524
87 6 GCCACCTAGT GGGGGGGGGG 2487933 0.008536 0.000630 0.062954 0.007863
88 6 CCGATAGTAG GGGGGGGGGG 2392073 0.008207 0.000605 0.060529 0.007560
89 6 AGAAGACCTA GGGGGGGGGG 2097662 0.007197 0.000531 0.053079 0.006630
90 7 GAGCAGCTTG GGGGGGGGGG 6702560 0.022831 0.001689 0.168858 0.021184
91 7 AGATTAGCGT GGGGGGGGGG 5508059 0.018762 0.001388 0.138765 0.017409
92 7 TATCCGCGTC GGGGGGGGGG 5015938 0.017086 0.001264 0.126367 0.015853
93 7 ATGACAGCAC GGGGGGGGGG 4212559 0.014349 0.001061 0.106127 0.013314
94 7 CCTAAGCTGA GGGGGGGGGG 4158571 0.014165 0.001048 0.104767 0.013144
95 7 TGGAGATCGA GGGGGGGGGG 3497305 0.011913 0.000881 0.088108 0.011054
96 7 ACGAGACGTC GGGGGGGGGG 3259877 0.011104 0.000821 0.082126 0.010303
97 7 CCAGTCGTCA GGGGGGGGGG 2958389 0.010077 0.000745 0.074531 0.009350
98 7 TAACTTGGTC GGGGGGGGGG 2807282 0.009562 0.000707 0.070724 0.008873
99 7 TCCAACTGAA GGGGGGGGGG 2789612 0.009502 0.000703 0.070279 0.008817
100 7 CAACTGCGCA GGGGGGGGGG 2788825 0.009500 0.000703 0.070259 0.008814
101 7 GCCACCTAGT GGGGGGGGGG 2528852 0.008614 0.000637 0.063710 0.007993
102 7 CCGATAGTAG GGGGGGGGGG 2436517 0.008300 0.000614 0.061383 0.007701
103 7 AGAAGACCTA GGGGGGGGGG 2130169 0.007256 0.000537 0.053665 0.006733
104 8 GAGCAGCTTG GGGGGGGGGG 6702867 0.022719 0.001696 0.169641 0.021185
105 8 AGATTAGCGT GGGGGGGGGG 5609892 0.019014 0.001420 0.141979 0.017731
106 8 TATCCGCGTC GGGGGGGGGG 5015601 0.017000 0.001269 0.126938 0.015852
107 8 ATGACAGCAC GGGGGGGGGG 4275974 0.014493 0.001082 0.108219 0.013515
108 8 CCTAAGCTGA GGGGGGGGGG 4227752 0.014330 0.001070 0.106999 0.013362
109 8 TGGAGATCGA GGGGGGGGGG 3480219 0.011796 0.000881 0.088080 0.011000
110 8 ACGAGACGTC GGGGGGGGGG 3239709 0.010981 0.000820 0.081993 0.010239
111 8 CCAGTCGTCA GGGGGGGGGG 3009166 0.010199 0.000762 0.076158 0.009511
112 8 TAACTTGGTC GGGGGGGGGG 2839678 0.009625 0.000719 0.071868 0.008975
113 8 TCCAACTGAA GGGGGGGGGG 2788726 0.009452 0.000706 0.070579 0.008814
114 8 CAACTGCGCA GGGGGGGGGG 2732388 0.009261 0.000692 0.069153 0.008636
115 8 GCCACCTAGT GGGGGGGGGG 2492932 0.008450 0.000631 0.063093 0.007879
116 8 CCGATAGTAG GGGGGGGGGG 2395457 0.008119 0.000606 0.060626 0.007571
117 8 AGAAGACCTA GGGGGGGGGG 2078966 0.007047 0.000526 0.052616 0.006571
5. BARCODES WITH HIGH MISMATCH RATES
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No barcodes found with high mismatch rates
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FINAL STATUS CHECK
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Overall % Undetermined: 7.58%
Status: WARNING
Reason(s):
Overall undetermined 7.58% >= 5.0%