FastQCFastQC Report
Mon 8 Jun 2026
NTC_0001_0001_A23YTGFLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameNTC_0001_0001_A23YTGFLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91113
Total Bases13.7 Mbp
Sequences flagged as poor quality0
Sequence length151
%GC77

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGG6691473.44067257142231TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTAGG85309.362001031685928TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGGGG34153.748093027339677TruSeq Adapter, Index 2 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGG11101.2182674261631161TruSeq Adapter, Index 2 (97% over 35bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGG8660.9504681000515843TruSeq Adapter, Index 2 (97% over 36bp)
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA4860.5334035757795265No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCGGGG4450.488404508687015TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTAGGGGG4040.4434054415945035TruSeq Adapter, Index 2 (96% over 33bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGT3520.3863334540625377TruSeq Adapter, Index 2 (97% over 36bp)
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG3030.33255408119587765No Hit
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC2840.3117008549822748No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGGG2740.300725472764589TruSeq Adapter, Index 2 (97% over 34bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATGTGGG2430.26670178788976323TruSeq Adapter, Index 2 (97% over 36bp)
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT2320.25462886745030894No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATTTGGG2110.23158056479316894TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCGAGG1730.18987411236596313TruSeq Adapter, Index 2 (97% over 36bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTAGT1600.1756061154829717TruSeq Adapter, Index 2 (97% over 36bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGGGTG1420.1558504274911374TruSeq Adapter, Index 2 (96% over 33bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTAGGG1240.13609473949930306TruSeq Adapter, Index 2 (97% over 35bp)
GNTCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGG1080.11853412795100589TruSeq Adapter, Index 7 (97% over 35bp)
GATCGGAAGAGCACACGTCTGAACTCAGTCACCAAGACTCAAATCTGGGG1010.11085136039862588Illumina Multiplexing PCR Primer 2.01 (96% over 26bp)
AGATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTAG970.1064612075115516TruSeq Adapter, Index 2 (97% over 36bp)
AATCGGAAGAGCACACGTCTGAACTCCAGTCACCAAGACTCAAATCTGGG930.1020710546244773TruSeq Adapter, Index 7 (97% over 35bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)