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aih-tih-sc-0754d2-R1_A23YTGFLT4_1.fastp.log
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s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0021/A23YTGFLT4/nfcore_rnafusion__73d51683--20260607-225254/fastp/aih-tih-sc-0754d2-R1_A23YTGFLT4_1.fastp.log
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1.6 KB
Published
Jun 08, 2026 12:21 AM
Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA

Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT

Read1 before filtering:
total reads: 30429186
total bases: 4594807086
Q20 bases: 4436453530(96.5536%)
Q30 bases: 4194451948(91.2868%)

Read2 before filtering:
total reads: 30429186
total bases: 4594807086
Q20 bases: 4463594163(97.1443%)
Q30 bases: 4211992843(91.6685%)

Read1 after filtering:
total reads: 29706015
total bases: 3243555507
Q20 bases: 3227942002(99.5186%)
Q30 bases: 3160168473(97.4291%)

Read2 after filtering:
total reads: 29706015
total bases: 3254064563
Q20 bases: 3225788010(99.131%)
Q30 bases: 3137373427(96.414%)

Filtering result:
reads passed filter: 59412030
reads failed due to low quality: 1024758
reads failed due to too many N: 1926
reads failed due to too short: 419658
reads with adapter trimmed: 49718284
bases trimmed due to adapters: 2506898340

Duplication rate: 40.7038%

Insert size peak (evaluated by paired-end reads): 95

JSON report: aih-tih-sc-0754d2-R1_A23YTGFLT4_1.fastp.json
HTML report: aih-tih-sc-0754d2-R1_A23YTGFLT4_1.fastp.html

fastp --in1 aih-tih-sc-0754d2-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-0754d2-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-0754d2-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-0754d2-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-0754d2-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-0754d2-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 
fastp v0.23.4, time used: 96 seconds