FastQCFastQC Report
Mon 8 Jun 2026
aih-tih-sc-0754d2-R1_A23YTGFLT4_1_1.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenameaih-tih-sc-0754d2-R1_A23YTGFLT4_1_1.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30429186
Total Bases4.5 Gbp
Sequences flagged as poor quality0
Sequence length151
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGAATAGAATCTGGG1321950.4344348876108615TruSeq Adapter, Index 25 (97% over 38bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGAATAGAATCTAGG537240.1765541805817612TruSeq Adapter, Index 25 (97% over 38bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)