Detecting adapter sequence for read1...
>Illumina TruSeq Adapter Read 1
AGATCGGAAGAGCACACGTCTGAACTCCAGTCA
Detecting adapter sequence for read2...
>Illumina TruSeq Adapter Read 2
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT
Read1 before filtering:
total reads: 61714805
total bases: 9318935555
Q20 bases: 9010588461(96.6912%)
Q30 bases: 8561391144(91.8709%)
Read2 before filtering:
total reads: 61714805
total bases: 9318935555
Q20 bases: 9099122890(97.6412%)
Q30 bases: 8636650527(92.6785%)
Read1 after filtering:
total reads: 60743285
total bases: 6674791650
Q20 bases: 6644490796(99.546%)
Q30 bases: 6507614497(97.4954%)
Read2 after filtering:
total reads: 60743285
total bases: 6686971859
Q20 bases: 6639487229(99.2899%)
Q30 bases: 6474518184(96.8229%)
Filtering result:
reads passed filter: 121486570
reads failed due to low quality: 1428564
reads failed due to too many N: 3890
reads failed due to too short: 510586
reads with adapter trimmed: 101483537
bases trimmed due to adapters: 5028060681
Duplication rate: 42.0221%
Insert size peak (evaluated by paired-end reads): 97
JSON report: aih-tih-sc-598a93-R1_A23YTGFLT4_1.fastp.json
HTML report: aih-tih-sc-598a93-R1_A23YTGFLT4_1.fastp.html
fastp --in1 aih-tih-sc-598a93-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-598a93-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-598a93-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-598a93-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-598a93-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-598a93-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000
fastp v0.23.4, time used: 154 seconds