Detecting adapter sequence for read1... >Illumina TruSeq Adapter Read 1 AGATCGGAAGAGCACACGTCTGAACTCCAGTCA Detecting adapter sequence for read2... >Illumina TruSeq Adapter Read 2 AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGT Read1 before filtering: total reads: 53899860 total bases: 8138878860 Q20 bases: 7770936978(95.4792%) Q30 bases: 7220016103(88.7102%) Read2 before filtering: total reads: 53899860 total bases: 8138878860 Q20 bases: 7912020166(97.2127%) Q30 bases: 7436574713(91.371%) Read1 after filtering: total reads: 52859459 total bases: 5256643244 Q20 bases: 5228442407(99.4635%) Q30 bases: 5108751061(97.1866%) Read2 after filtering: total reads: 52859459 total bases: 5268926181 Q20 bases: 5234564842(99.3478%) Q30 bases: 5112608609(97.0332%) Filtering result: reads passed filter: 105718918 reads failed due to low quality: 1246834 reads failed due to too many N: 2844 reads failed due to too short: 831124 reads with adapter trimmed: 97990547 bases trimmed due to adapters: 5491336765 Duplication rate: 36.0829% Insert size peak (evaluated by paired-end reads): 88 JSON report: aih-tih-sc-fba77b-R1_A23YTGFLT4_1.fastp.json HTML report: aih-tih-sc-fba77b-R1_A23YTGFLT4_1.fastp.html fastp --in1 aih-tih-sc-fba77b-R1_A23YTGFLT4_1_1.fastq.gz --in2 aih-tih-sc-fba77b-R1_A23YTGFLT4_1_2.fastq.gz --out1 aih-tih-sc-fba77b-R1_A23YTGFLT4_1_1.fastp.fastq.gz --out2 aih-tih-sc-fba77b-R1_A23YTGFLT4_1_2.fastp.fastq.gz --json aih-tih-sc-fba77b-R1_A23YTGFLT4_1.fastp.json --html aih-tih-sc-fba77b-R1_A23YTGFLT4_1.fastp.html --thread 12 --detect_adapter_for_pe --reads_to_process 300000000 fastp v0.23.4, time used: 146 seconds