[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3282fd-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (11132525) splicing reads. 247662 splicing junctions. 181670 known splicing junctions. 65992 novel splicing junctions.
sampling 10% (22265050) splicing reads. 298523 splicing junctions. 193732 known splicing junctions. 104791 novel splicing junctions.
sampling 15% (33397575) splicing reads. 333095 splicing junctions. 200031 known splicing junctions. 133064 novel splicing junctions.
sampling 20% (44530100) splicing reads. 358739 splicing junctions. 203961 known splicing junctions. 154778 novel splicing junctions.
sampling 25% (55662625) splicing reads. 378931 splicing junctions. 206859 known splicing junctions. 172072 novel splicing junctions.
sampling 30% (66795150) splicing reads. 395543 splicing junctions. 209068 known splicing junctions. 186475 novel splicing junctions.
sampling 35% (77927675) splicing reads. 408982 splicing junctions. 210715 known splicing junctions. 198267 novel splicing junctions.
sampling 40% (89060200) splicing reads. 420287 splicing junctions. 212009 known splicing junctions. 208278 novel splicing junctions.
sampling 45% (100192725) splicing reads. 429871 splicing junctions. 213030 known splicing junctions. 216841 novel splicing junctions.
sampling 50% (111325250) splicing reads. 438106 splicing junctions. 213955 known splicing junctions. 224151 novel splicing junctions.
sampling 55% (122457775) splicing reads. 445196 splicing junctions. 214687 known splicing junctions. 230509 novel splicing junctions.
sampling 60% (133590300) splicing reads. 451164 splicing junctions. 215289 known splicing junctions. 235875 novel splicing junctions.
sampling 65% (144722825) splicing reads. 456283 splicing junctions. 215818 known splicing junctions. 240465 novel splicing junctions.
sampling 70% (155855350) splicing reads. 460591 splicing junctions. 216265 known splicing junctions. 244326 novel splicing junctions.
sampling 75% (166987875) splicing reads. 464467 splicing junctions. 216672 known splicing junctions. 247795 novel splicing junctions.
sampling 80% (178120400) splicing reads. 467529 splicing junctions. 216972 known splicing junctions. 250557 novel splicing junctions.
sampling 85% (189252925) splicing reads. 469936 splicing junctions. 217180 known splicing junctions. 252756 novel splicing junctions.
sampling 90% (200385450) splicing reads. 471879 splicing junctions. 217352 known splicing junctions. 254527 novel splicing junctions.
sampling 95% (211517975) splicing reads. 473273 splicing junctions. 217481 known splicing junctions. 255792 novel splicing junctions.
sampling 100% (222650500) splicing reads. 474147 splicing junctions. 217569 known splicing junctions. 256578 novel splicing junctions.