[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-31d31f-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (6938883) splicing reads. 204235 splicing junctions. 167076 known splicing junctions. 37159 novel splicing junctions.
sampling 10% (13877766) splicing reads. 229887 splicing junctions. 176392 known splicing junctions. 53495 novel splicing junctions.
sampling 15% (20816649) splicing reads. 244200 splicing junctions. 180778 known splicing junctions. 63422 novel splicing junctions.
sampling 20% (27755532) splicing reads. 253518 splicing junctions. 183321 known splicing junctions. 70197 novel splicing junctions.
sampling 25% (34694415) splicing reads. 260271 splicing junctions. 185057 known splicing junctions. 75214 novel splicing junctions.
sampling 30% (41633298) splicing reads. 265230 splicing junctions. 186165 known splicing junctions. 79065 novel splicing junctions.
sampling 35% (48572182) splicing reads. 269155 splicing junctions. 187075 known splicing junctions. 82080 novel splicing junctions.
sampling 40% (55511065) splicing reads. 272281 splicing junctions. 187778 known splicing junctions. 84503 novel splicing junctions.
sampling 45% (62449948) splicing reads. 274751 splicing junctions. 188292 known splicing junctions. 86459 novel splicing junctions.
sampling 50% (69388831) splicing reads. 276854 splicing junctions. 188717 known splicing junctions. 88137 novel splicing junctions.
sampling 55% (76327714) splicing reads. 278648 splicing junctions. 189077 known splicing junctions. 89571 novel splicing junctions.
sampling 60% (83266597) splicing reads. 280162 splicing junctions. 189371 known splicing junctions. 90791 novel splicing junctions.
sampling 65% (90205480) splicing reads. 281405 splicing junctions. 189593 known splicing junctions. 91812 novel splicing junctions.
sampling 70% (97144364) splicing reads. 282489 splicing junctions. 189788 known splicing junctions. 92701 novel splicing junctions.
sampling 75% (104083247) splicing reads. 283377 splicing junctions. 189959 known splicing junctions. 93418 novel splicing junctions.
sampling 80% (111022130) splicing reads. 284094 splicing junctions. 190098 known splicing junctions. 93996 novel splicing junctions.
sampling 85% (117961013) splicing reads. 284669 splicing junctions. 190186 known splicing junctions. 94483 novel splicing junctions.
sampling 90% (124899896) splicing reads. 285086 splicing junctions. 190268 known splicing junctions. 94818 novel splicing junctions.
sampling 95% (131838779) splicing reads. 285419 splicing junctions. 190320 known splicing junctions. 95099 novel splicing junctions.
sampling 100% (138777663) splicing reads. 285628 splicing junctions. 190361 known splicing junctions. 95267 novel splicing junctions.