File Info

Filename
.command.log
Full Path
s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9d/bacb5ddad45aa89e83a2f8717ba1a2/.command.log
Size
61.1 KB
Task
NFCORE_RNAFUSION:RNAFUSION:FUSIONINSPECTOR_WORKFLOW:FUSIONINSPECTOR (aih-tih-sc-45523c-R1_A23YTGFLT4_1)
Attempt

Content

View in new tab 
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/c1424313aaf74f05a9d9fc6e6acbbc/aih-tih-sc-45523c-R1_A23YTGFLT4_1_2.fastp.fastq.gz
  Downloading: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9d/bacb5ddad45aa89e83a2f8717ba1a2/.command.sh
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/9d/bacb5ddad45aa89e83a2f8717ba1a2/.command.run
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/6a/c9c88734d60ae422e0ad924c1a7c9b/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusionreport.tsv
  Downloading: s3://natera-rnd-pltf-dev-nextflow-scratch-01/work/97/c1424313aaf74f05a9d9fc6e6acbbc/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz
==> STAGING COMPLETE (6 inputs)

Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusionreport.tsv --gtf /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1 --shrink_introns --max_intron_length 1000 
Done.
Execution Time = 0.09 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_pair_to_mini_genome_join.pl --fusions aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusionreport.tsv --gtf /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_annot.gtf --genome_fa /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_genome.fa --out_prefix /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1 --shrink_introns --max_intron_length 1000 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/fusion_contigs.ok
Running: cp /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/cp_contigs_file_workdir
Running: cp /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/cp_gtf_file_workdir.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf > /tmp/nxf.YGSl4tcF7M/cytoBand.txt
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fasta_and_gtf_to_cytoband.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf > /tmp/nxf.YGSl4tcF7M/cytoBand.txt
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/cytoband.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed
-parsing GTF file: /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/gtf_gene_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/merged_contig_gtf_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.sorted.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.bedsort.ok
Running: bgzip -f /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.sorted.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.bgzip.ok
Running: tabix -p bed /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.sorted.bed.gz
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.bed.tabix.ok
Running: samtools faidx /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa
Execution Time = 0.00 minutes. CMD: samtools faidx /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/merged_contig_fai.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --CPU 8 --out_prefix aih-tih-sc-45523c-R1_A23YTGFLT4_1.star --out_dir /tmp/nxf.YGSl4tcF7M/fi_workdir --reads "/tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_2.fastp.fastq.gz"
* Running CMD: /usr/local/bin/STAR  --runThreadN 8  --genomeDir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_genome.fa.star.idx  --outSAMtype BAM SortedByCoordinate  --twopassMode None  --alignSJDBoverhangMin 10  --genomeSuffixLengthMax 10000 --limitBAMsortRAM 47271261705  --alignInsertionFlush Right   --alignMatesGapMax 100000  --alignIntronMax 100000  --outSAMattributes All  --readFilesIn /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_2.fastp.fastq.gz  --genomeFastaFiles /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa  --outSAMfilter KeepAllAddedReferences  --sjdbGTFfile /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  --alignSJstitchMismatchNmax 5 -1 5 5  --scoreGapNoncan -6  --readFilesCommand 'gunzip -c' 
Jun 08 09:35:35 ..... started STAR run
Jun 08 09:35:35 ..... loading genome
Jun 08 09:36:40 ... generating Suffix Array index
Jun 08 09:40:20 ... completed Suffix Array index
Jun 08 09:40:20 ..... processing annotations GTF
Jun 08 09:40:20 ..... inserting junctions into the genome indices
Jun 08 09:41:05 ..... started mapping
Jun 08 10:25:17 ..... finished mapping
Jun 08 10:25:18 ..... started sorting BAM
Jun 08 10:25:18 ..... finished successfully
* Running CMD: mv Aligned.sortedByCoord.out.bam aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
* Running CMD: samtools index aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam
Execution Time = 49.74 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/run_FI_STAR.pl --genome /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir/ref_genome.fa --patch /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa --max_mate_dist 100000 -G /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --CPU 8 --out_prefix aih-tih-sc-45523c-R1_A23YTGFLT4_1.star --out_dir /tmp/nxf.YGSl4tcF7M/fi_workdir --reads "/tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_2.fastp.fastq.gz"
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/run_STAR.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py  -i /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam   -o /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam   --remove_dups 
10:25:23 : INFO : Done.
Execution Time = 0.07 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/bam_mark_duplicates.py  -i /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.sortedByCoord.out.bam   -o /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam   --remove_dups 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/mark_dup_reads.ok
Running: samtools index /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam
Execution Time = 0.01 minutes. CMD: samtools index /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/mark_dups_reads.index.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
-parsing GTF file: /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf
-parsing /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam

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[1170000]   -done parsing /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.  Extracting junction info.
junction read elimination tally: $VAR1 = {
          'num_hits: 2 != num_counted_on_fusion_contigs 1 ' => 53,
          'num genes matched < 2' => 12,
          'num_hits: 4 != num_counted_on_fusion_contigs 3 ' => 6,
          'num_hits: 4 != num_counted_on_fusion_contigs 2 ' => 20,
          'num_hits: 3 != num_counted_on_fusion_contigs 2 ' => 8,
          'small anchor length' => 1,
          'num_hits: 5 != num_counted_on_fusion_contigs 3 ' => 21,
          'seq-similar region overlap' => 98,
          'num_hits: 3 != num_counted_on_fusion_contigs 1 ' => 15,
          'excessive soft clipping' => 7695,
          'low complexity anchor region' => 2,
          'per_id < 96' => 4603,
          ' ** passed ** ' => 3210
        };
-writing fusion junction support info.
Execution Time = 0.41 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_JUNCTION_reads_from_fusion_contig_bam.pl --gtf_file /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/get_fusion_JUNCTION_reads_from_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir   > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
-outputting the spanning read info: /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info.
 - counting read alignments among fusion contigs.

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[6000]   -fusion SPANNING read extraction for scaff: AC092807.3--DDAH1

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[83000]   -fusion SPANNING read extraction for scaff: CPSF7--PIEZO2

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[24000]   -fusion SPANNING read extraction for scaff: CPSF7--AP005139.1

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[168000]   -fusion SPANNING read extraction for scaff: DDX5--SMURF2

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[45000]   
[46000]   -fusion SPANNING read extraction for scaff: DHX38--WWOX

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[58000]   
[59000]   -fusion SPANNING read extraction for scaff: ERC1--RASSF7

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[50000]   
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[52000]   
[53000]   
[54000]   
[55000]   
[56000]   
[57000]   
[58000]   -fusion SPANNING read extraction for scaff: FSIP1--AC013652.1

[1000]   
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[6000]   
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[14000]   
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[50000]   
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[53000]   
[54000]   
[55000]   
[56000]   
[57000]   
[58000]   
[59000]   
[60000]   
[61000]   -fusion SPANNING read extraction for scaff: KIAA1958--NTRK2

[1000]   
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[6000]   
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[8000]   
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[12000]   
[13000]   
[14000]   
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[44000]   
[45000]   
[46000]   
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[48000]   
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[50000]   
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[52000]   
[53000]   
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[59000]   
[60000]   
[61000]   
[62000]   
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[65000]   
[66000]   
[67000]   
[68000]   
[69000]   
[70000]   
[71000]   
[72000]   
[73000]   
[74000]   -fusion SPANNING read extraction for scaff: LMNTD2--ERC1

[1000]   
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[4000]   
[5000]   
[6000]   
[7000]   
[8000]   
[9000]   -fusion SPANNING read extraction for scaff: NAE1--CAPNS2

[1000]   
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[3000]   
[4000]   
[5000]   
[6000]   
[7000]   
[8000]   
[9000]   
[10000]   
[11000]   
[12000]   
[13000]   
[14000]   
[15000]   
[16000]   
[17000]   
[18000]   
[19000]   
[20000]   -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC2

[1000]   
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[3000]   
[4000]   
[5000]   
[6000]   
[7000]   
[8000]   
[9000]   
[10000]   
[11000]   
[12000]   
[13000]   
[14000]   
[15000]   
[16000]   
[17000]   
[18000]   
[19000]   
[20000]   -fusion SPANNING read extraction for scaff: PCDHA11--PCDHAC1

[1000]   
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[3000]   
[4000]   
[5000]   
[6000]   
[7000]   
[8000]   
[9000]   
[10000]   
[11000]   
[12000]   
[13000]   
[14000]   
[15000]   
[16000]   
[17000]   
[18000]   
[19000]   
[20000]   
[21000]   
[22000]   
[23000]   -fusion SPANNING read extraction for scaff: PCDHA11--PCDHA13

[1000]   
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[4000]   
[5000]   
[6000]   
[7000]   
[8000]   
[9000]   
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[11000]   
[12000]   
[13000]   
[14000]   
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[16000]   
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[20000]   
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[30000]   
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[40000]   
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[42000]   
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[44000]   
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[50000]   
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[52000]   
[53000]   
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[60000]   
[61000]   
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[65000]   
[66000]   
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[68000]   
[69000]   
[70000]   
[71000]   
[72000]   
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[75000]   
[76000]   -fusion SPANNING read extraction for scaff: STX16--NPEPL1

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[8000]   
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[14000]   
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[18000]   
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[20000]   
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[22000]   
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[25000]   
[26000]   -fusion SPANNING read extraction for scaff: TLK2--AC240565.1

[1000]   
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[4000]   
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[6000]   
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[8000]   
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[14000]   
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[29000]   -fusion SPANNING read extraction for scaff: TLK2--FAM157A

[1000]   
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[5000]   -fusion SPANNING read extraction for scaff: TLK2P1--AC110079.1

[1000]   
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[19000]   -fusion SPANNING read extraction for scaff: TVP23C--CDRT4

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[25000]   
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[29000]   
[30000]   -fusion SPANNING read extraction for scaff: TXNL4B--CTCF

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[50000]   
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[53000]   
[54000]   
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[57000]   
[58000]   -fusion SPANNING read extraction for scaff: TXNL4B--CARMIL2

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[69000]   
[70000]   
[71000]   
[72000]   
[73000]   -fusion SPANNING read extraction for scaff: WWP2--MPHOSPH6

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[183000]   
[184000]   
[185000]   
[186000]   
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[188000]   
[189000]   
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[192000]   
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[199000]   
[200000]   
[201000]   
[202000]   
[203000]   -fusion SPANNING read extraction for scaff: ZFHX3--CNTNAP4
-filtered reads reasons: $VAR1 = {
          'lacks exon overlap' => 47046,
          'seq similar region alignment' => 19027
        };
Execution Time = 1.17 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_fusion_SPANNING_reads_from_bam.from_chim_summary.pl --gtf_file /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf --MIN_ALIGN_PER_ID 96 --bam /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam --junction_info /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info --genome_lib_dir ctat_genome_lib_build_dir   > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/get_fusion_SPANNING_reads_from_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/coalesce_junction_and_spanning_info.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junction_info /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_spanning_info 1  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/coalesce_junc_n_span.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj
EM: Starting log likelihood: -3012.042627
EM: Round [1] log likelihood: -3011.682253
EM: Round [2] log likelihood: -3011.396539
EM: Round [3] log likelihood: -3011.133574
EM: Round [4] log likelihood: -3010.890806
EM: Round [5] log likelihood: -3010.666324
EM: Round [6] log likelihood: -3010.458448
EM: Round [7] log likelihood: -3010.265695
EM: Round [8] log likelihood: -3010.086749
EM: Round [9] log likelihood: -3009.920437
EM: Round [10] log likelihood: -3009.765713
EM: Round [11] log likelihood: -3009.621635
EM: Round [12] log likelihood: -3009.487357
EM: Round [13] log likelihood: -3009.362113
EM: Round [14] log likelihood: -3009.245211
EM: Round [15] log likelihood: -3009.136023
EM: Round [16] log likelihood: -3009.033976
EM: Round [17] log likelihood: -3008.938549
EM: Round [18] log likelihood: -3008.849264
EM: Round [19] log likelihood: -3008.765686
EM: Round [20] log likelihood: -3008.687413
EM: Round [21] log likelihood: -3008.614078
EM: Round [22] log likelihood: -3008.545341
EM: Round [23] log likelihood: -3008.480891
EM: Round [24] log likelihood: -3008.420439
EM: Round [25] log likelihood: -3008.363719
EM: Round [26] log likelihood: -3008.310485
EM: Round [27] log likelihood: -3008.260508
EM: Round [28] log likelihood: -3008.213576
EM: Round [29] log likelihood: -3008.169494
EM: Round [30] log likelihood: -3008.128079
EM: Round [31] log likelihood: -3008.089161
EM: Round [32] log likelihood: -3008.052582
EM: Round [33] log likelihood: -3008.018195
EM: Round [34] log likelihood: -3007.985863
EM: Round [35] log likelihood: -3007.955458
EM: Round [36] log likelihood: -3007.926861
EM: Round [37] log likelihood: -3007.899960
EM: Round [38] log likelihood: -3007.874652
EM: Round [39] log likelihood: -3007.850838
EM: Round [40] log likelihood: -3007.828429
EM: Round [41] log likelihood: -3007.807338
EM: Round [42] log likelihood: -3007.787487
EM: Round [43] log likelihood: -3007.768800
EM: Round [44] log likelihood: -3007.751207
EM: Round [45] log likelihood: -3007.734643
EM: Round [46] log likelihood: -3007.719047
EM: Round [47] log likelihood: -3007.704360
EM: Round [48] log likelihood: -3007.690529
EM: Round [49] log likelihood: -3007.677503
EM: Round [50] log likelihood: -3007.665234
EM: Round [51] log likelihood: -3007.653678
EM: Round [52] log likelihood: -3007.642792
EM: Round [53] log likelihood: -3007.632538
EM: Round [54] log likelihood: -3007.622877
EM: Round [55] log likelihood: -3007.613776
EM: Round [56] log likelihood: -3007.605201
EM: Round [57] log likelihood: -3007.597121
EM: Round [58] log likelihood: -3007.589509
EM: Round [59] log likelihood: -3007.582335
EM: Round [60] log likelihood: -3007.575576
EM: Round [61] log likelihood: -3007.569206
EM: Round [62] log likelihood: -3007.563203
EM: Round [63] log likelihood: -3007.557546
EM: Round [64] log likelihood: -3007.552215
EM: Round [65] log likelihood: -3007.547191
EM: Round [66] log likelihood: -3007.542455
EM: Round [67] log likelihood: -3007.537992
EM: Round [68] log likelihood: -3007.533786
EM: Round [69] log likelihood: -3007.529821
EM: Round [70] log likelihood: -3007.526084
EM: Round [71] log likelihood: -3007.522562
EM: Round [72] log likelihood: -3007.519241
EM: Round [73] log likelihood: -3007.516112
EM: Round [74] log likelihood: -3007.513162
EM: Round [75] log likelihood: -3007.510381
EM: Round [76] log likelihood: -3007.507760
EM: Round [77] log likelihood: -3007.505289
EM: Round [78] log likelihood: -3007.502959
EM: Round [79] log likelihood: -3007.500763
EM: Round [80] log likelihood: -3007.498693
EM: Round [81] log likelihood: -3007.496742
EM: Round [82] log likelihood: -3007.494902
EM: Round [83] log likelihood: -3007.493168
EM: Round [84] log likelihood: -3007.491533
EM: Round [85] log likelihood: -3007.489992
EM: Round [86] log likelihood: -3007.488539
EM: Round [87] log likelihood: -3007.487169
EM: Round [88] log likelihood: -3007.485878
EM: Round [89] log likelihood: -3007.484661
EM: Round [90] log likelihood: -3007.483513
EM: Round [91] log likelihood: -3007.482431
EM: Round [92] log likelihood: -3007.481410
EM: Round [93] log likelihood: -3007.480449
EM: Round [94] log likelihood: -3007.479542
EM: Round [95] log likelihood: -3007.478687
EM: Round [96] log likelihood: -3007.477881
EM: Round [97] log likelihood: -3007.477121
EM: Round [98] log likelihood: -3007.476405
EM: Round [99] log likelihood: -3007.475729
EM: Round [100] log likelihood: -3007.475093
EM: Round [101] log likelihood: -3007.474492
EM: Round [102] log likelihood: -3007.473926
EM: Round [103] log likelihood: -3007.473392
EM: Round [104] log likelihood: -3007.472889
EM: Round [105] log likelihood: -3007.472415
EM: Round [106] log likelihood: -3007.471968
EM: Round [107] log likelihood: -3007.471546
EM: Round [108] log likelihood: -3007.471148
EM: Round [109] log likelihood: -3007.470774
EM: Round [110] log likelihood: -3007.470420
EM: Round [111] log likelihood: -3007.470087
EM: Round [112] log likelihood: -3007.469773
EM: Round [113] log likelihood: -3007.469477
EM: Round [114] log likelihood: -3007.469197
EM: Round [115] log likelihood: -3007.468934
EM: Round [116] log likelihood: -3007.468686
EM: Round [117] log likelihood: -3007.468452
EM: Round [118] log likelihood: -3007.468231
EM: Round [119] log likelihood: -3007.468023
EM: Round [120] log likelihood: -3007.467827
EM: Round [121] log likelihood: -3007.467642
EM: Round [122] log likelihood: -3007.467467
EM: Round [123] log likelihood: -3007.467303
EM: Round [124] log likelihood: -3007.467148
EM: Round [125] log likelihood: -3007.467002
EM: Round [126] log likelihood: -3007.466864
EM: Round [127] log likelihood: -3007.466734
EM: Round [128] log likelihood: -3007.466611
EM: Round [129] log likelihood: -3007.466496
EM: Round [130] log likelihood: -3007.466387
EM: Round [131] log likelihood: -3007.466284
EM: Round [132] log likelihood: -3007.466188
EM: Stopping iterations at round 132 due to insufficient improvement in likelihood.
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/init_EM_adj_counts.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/filter_fusions_by_frag_thresholds.pl --min_junction_reads 0 --min_sum_frags 1 --min_novel_junction_support 3 --min_spanning_frags_only 5 --fusion_preds /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj --require_LDAS 1 > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/filter_by_frag_threshs.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,JunctionReads  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_extract_junc_reads.ok
Running: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam"
[bam_sort_core] merging from 0 files and 8 in-memory blocks...
Execution Time = 0.00 minutes. CMD: bash -c "set -eof pipefail; /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_junction_reads_by_accession.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_junction_read_accs /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_junc_reads.sam | samtools view -@ 8 -bT /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam"
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_junc_reads_bam.ok
Running: samtools index /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam
Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/samtools_idx_junc_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bam_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bedsort.ok
Running: bgzip -f /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bgzip.ok
Running: tabix -p bed /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.bed.sorted.bed.gz
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.junction_reads.bam.tabix.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_extractions.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary LeftGene,RightGene,SpanningFrags  > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/span_reads_acc.ok
Running: samtools view -H /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam
Execution Time = 0.00 minutes. CMD: samtools view -H /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam -b -o /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/init_spanning_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam || : 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/retrieve_fusion_spanning_reads_by_accession.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.fusion_spanning_read_accs /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam.fusion_span_reads.sam | samtools view -@ 8 -bT /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa -  | samtools sort -@ 8 - -o /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam || : 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_spanning_reads.ok
Running: samtools index /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam
Execution Time = 0.00 minutes. CMD: samtools index /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/samtools_index_span_reads_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_pair_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bam_to_bed.ok
Running: sort -k1,1 -k2,2n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: sort -k1,1 -k2,2n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bedsort.ok
Running: bgzip -f /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Execution Time = 0.00 minutes. CMD: bgzip -f /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bgzip.ok
Running: tabix -p bed /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed.gz
Execution Time = 0.00 minutes. CMD: tabix -p bed /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.bed.sorted.bed.gz
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.spanning_reads.bam.tabix.ok
Running: cp /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.star.cSorted.dupsMarked.bam /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/cp_consol_bam.ok
Running: samtools index /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam
Execution Time = 0.01 minutes. CMD: samtools index /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/index_consol_bam.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam --max_insert_size 10000000 
-extracting read coordinates from /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam into /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords

CMD: touch /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords.ok
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k2,2 -k4,4n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.read_coords.sort_by_readname
CMD: /usr/bin/sort --parallel=1 -S10G -T . -k1,1 -k3,3n /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.frag_coords.coord_sorted
Execution Time = 0.20 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/SAM_to_frag_coords.pl --sam /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam --max_insert_size 10000000 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_sam_frag_coordss.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.FusionJuncSpan
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_summary_to_igv_JuncSpan_fmt.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.consolidated.bam.frag_coords > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.FusionJuncSpan
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_juncspan_fmt.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.Pfam.gff3 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_pfam_domain_info.pl --finspector_gtf /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.Pfam.gff3 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_pfam_gff3.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.Pfam.gff3 > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.Pfam.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.Pfam.gff3 > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.Pfam.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_pfam_bed.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/get_seq_similar_region_FI_coordinates.pl --finspector_gtf /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  --genome_lib_dir ctat_genome_lib_build_dir  > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_seqsim_gff3.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.seqsimilar.bed
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/transcript_gff3_to_bed.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.seqsimilar.gff3 > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.igv.seqsimilar.bed
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/prep_igv_seqsim_bed.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/append_breakpoint_junction_info_via_FI_contigs.pl /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa > /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/add_splice_info.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl  --fusion_preds /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/blast_filter.pl --fusion_preds /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --genome_lib_dir ctat_genome_lib_build_dir
CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionFilter/util/promiscuity_filter.pl --fusion_preds /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter  --genome_lib_dir ctat_genome_lib_build_dir  --max_promiscuity 10  --min_pct_dom_promiscuity 50 
CMD: cp /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo.post_blast_filter.post_promisc_filter /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/blast_and_promiscuity_filter.pl  --fusion_preds /tmp/nxf.YGSl4tcF7M/fi_workdir/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_preds.coalesced.summary.EMadj.min_frag_thresh.wSpliceInfo --out_prefix /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1 --genome_lib_dir ctat_genome_lib_build_dir --max_promiscuity 10 --min_pct_dom_promiscuity 50
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/blast_filter.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl  --fusions /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir
-done, see /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/../FusionFilter/util/filter_by_annotation_rules.pl  --fusions /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter --genome_lib_dir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/annot_filter.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj
EM: Starting log likelihood: -2836.006779
EM: Round [1] log likelihood: -2836.006748
EM: Stopping iterations at round 1 due to insufficient improvement in likelihood.
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_EM_runner.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/EM_adj_counts.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM
-total frags in /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz: 241112810
Execution Time = 3.40 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/incorporate_FFPM_into_final_report.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1_1.fastp.fastq.gz /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/add_FFPM.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa --gtf /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  > /tmp/nxf.YGSl4tcF7M/fi_workdir/microH.dat
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/find_microhomologies_by_kmer_matches.pl --fasta /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fa --gtf /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gtf  > /tmp/nxf.YGSl4tcF7M/fi_workdir/microH.dat
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/microH.dat.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.YGSl4tcF7M/fi_workdir/microH.dat /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM  >  /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/misc/append_microH_distance.py /tmp/nxf.YGSl4tcF7M/fi_workdir/microH.dat /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM  >  /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/append_microH_info.ok
Running: cp /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv
Execution Time = 0.00 minutes. CMD: cp /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.post_blast_and_promiscuity_filter.annot_filter.pass.EMadj.FFPM.wMicroH /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/cp_final.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads  > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/column_exclusions.pl /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.tsv JunctionReads,SpanningFrags,CounterFusionLeftReads,CounterFusionRightReads  > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/final.abridged.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.YGSl4tcF7M --json_outfile /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_inspector_web.json --file_prefix aih-tih-sc-45523c-R1_A23YTGFLT4_1
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/util/create_fusion_inspector_igvjs.py --fusion_inspector_directory /tmp/nxf.YGSl4tcF7M --json_outfile /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_inspector_web.json --file_prefix aih-tih-sc-45523c-R1_A23YTGFLT4_1
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/create_fi_igvjs.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_inspector_web.json --input_file_prefix aih-tih-sc-45523c-R1_A23YTGFLT4_1 --html_output /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_inspector_web.html
Warning - not locating file: /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.gmap_trinity_GG.fusions.gff3.bed
Execution Time = 0.01 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/fusion-reports/create_fusion_report.py --html_template /usr/local/src/STAR-Fusion/FusionInspector/util/fusion_report_html_template/igvjs_fusion.html --fusions_json /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_inspector_web.json --input_file_prefix aih-tih-sc-45523c-R1_A23YTGFLT4_1 --html_output /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.fusion_inspector_web.html
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/fusion_reports_html.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv  --genome_lib_dir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated 
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/FusionAnnotator --annotate /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv  --genome_lib_dir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/fusion_annotator.ok
Running: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated  --genome_lib_dir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect 
WARNING, no entry stored in dbm for [ENSG00000226049.3]
WARNING, no entry stored in dbm for [ENSG00000260404.3]
WARNING, no entry stored in dbm for [ENSG00000280136.2]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000282057.1]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
WARNING, no entry stored in dbm for [ENSG00000259345.7]
Execution Time = 0.00 minutes. CMD: /usr/local/src/STAR-Fusion/FusionInspector/FusionAnnotator/util/fusion_to_coding_region_effect.pl --fusions /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated  --genome_lib_dir /tmp/nxf.YGSl4tcF7M/ctat_genome_lib_build_dir > /tmp/nxf.YGSl4tcF7M/aih-tih-sc-45523c-R1_A23YTGFLT4_1.FusionInspector.fusions.abridged.tsv.annotated.coding_effect 
Running: touch /tmp/nxf.YGSl4tcF7M/chckpts_dir/fusion_coding_region_effect.ok
ls: cannot access 'IGV_inputs': No such file or directory