MultiQC Report

A modular tool to aggregate results from bioinformatics analyses across many samples into a single report.

This report has been generated by the nf-core/rnafusion analysis pipeline. For information about how to interpret these results, please see the documentation.

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Report generated on 2026-06-08, 13:04 UTC based on data in: /tmp/nxf.CkOIzMyWsy

General Statistics

Showing ⁴⁸⁰/₄₈₀ rows and ⁵⁵/₆₁ columns.

Sample Name	Total Reads	Expected Distinct	Unique Reads	Frac Antisense	RNA contaminated	RNA contamination %	Reads	Frac Unmapped	Frac Dupes	Unique Reads	Frac Reads On Target	Frac Pairs On Target	Frac Reads Off Target	Frac Pairs Off Target	Reads On Target	Pairs On Target	Reads Off Target	Pairs Off Target	Mean Depth	Q50 Depth	Frac Bases Gt30X	Frac Bases Gt100X	N Q30	GC Bias	Fragment Length Mean	Fragment Length Median	pct Mito est. counts	pct Ribo est. counts	pct Housekeeping est. counts	pct Male est. counts	pct Mito TPM	pct Ribo TPM	pct Housekeeping TPM	pct Male TPM	ChrY Signature Score	Frag Length	% Aligned	M Aligned	Duplication	% Proper Pairs	% Aligned	M Aligned	% Duplication	% > Q30	Mb Q30 bases	M Reads After Filtering	GC content	% PF	% Adapter	% Dups	% GC	Average Read Length	Median Read Length	% Failed	M Seqs	% Dups	% GC	Average Read Length	Median Read Length	% Failed	M Seqs
FFPE_HD789_01_RNA_13_A23YTGFLT4_1	9999000000	475000445	575516536	0.9471	Yes	63.13%	592637302	0.00%	0.43%	339278754	0.94%	0.97%	0.05%	0.03%	320056253	162362031	16718859	5088078	685.83	288.00	0.72%	0.62%	0.97	0.000	164.0bp	147.0bp	0.02%	1.65%	0.65%	0.00%	0.05%	4.73%	0.93%	0.00%	4.06				42.9%	90.9%	96.4%	285.7M	30.7%	97.2%	77090.7Mb	592.6M	54.6%	98.8%	43.8%
FFPE_HD789_01_RNA_13_A23YTGFLT4_1_1																																				164.0bp	97.9%	290.2M												86.8%	53%	151bp	151bp	18%	458.1M
FFPE_HD789_01_RNA_13_A23YTGFLT4_1_2																																																		86.3%	54%	151bp	151bp	18%	458.1M
FFPE_HD789_01_RNA_13_A23YTGFLT4_1_trimmed_1																																																		84.2%	54%	134bp	151bp	9%	296.3M	84.2%	54%	134bp	151bp	9%	296.3M
FFPE_HD789_01_RNA_13_A23YTGFLT4_1_trimmed_2																																																		84.7%	54%	134bp	151bp	9%	296.3M	84.7%	54%	134bp	151bp	9%	296.3M
FFPE_V4_0002_RNA_0002_A23YTGFLT4_1	9999000000	270627745	552660689	0.9621	No	0.00%	573059634	0.01%	0.54%	266013065	0.92%	0.95%	0.06%	0.05%	245562321	124568505	17171336	6066186	489.74	122.00	0.59%	0.52%	0.97	0.000	152.7bp	138.0bp	0.02%	1.65%	0.99%	0.00%	0.04%	4.49%	1.37%	0.00%	6.70				53.9%	88.6%	95.7%	274.1M	37.2%	97.2%	72826.9Mb	573.1M	55.3%	98.8%	50.5%
FFPE_V4_0002_RNA_0002_A23YTGFLT4_1_1																																				152.7bp	96.5%	276.4M												85.6%	54%	151bp	151bp	18%	290.1M
FFPE_V4_0002_RNA_0002_A23YTGFLT4_1_2																																																		84.9%	55%	151bp	151bp	18%	290.1M
FFPE_V4_0002_RNA_0002_A23YTGFLT4_1_trimmed_1																																																		85.6%	54%	131bp	147bp	9%	286.5M	85.6%	54%	131bp	147bp	9%	286.5M
FFPE_V4_0002_RNA_0002_A23YTGFLT4_1_trimmed_2																																																		85.9%	55%	131bp	147bp	9%	286.5M	85.9%	55%	131bp	147bp	9%	286.5M
NTC_0001_0001_A23YTGFLT4_1	9999000000	18766	1111	0.4541	Skipped	N/A%	7794	0.50%	0.15%	6598	0.02%	0.05%	0.38%	0.95%	140	69	2528	1225	0.00	0.00	0.00%	0.00%	0.84	0.000	39.4bp	31.0bp	0.00%	0.00%	0.21%	0.00%	0.00%	0.00%	0.48%	0.00%	0.00				31.0%	2.4%	9.3%	0.0M	47.3%	83.3%	0.7Mb	0.0M	68.7%	4.3%	51.4%
NTC_0001_0001_A23YTGFLT4_1_1																																				39.4bp	12.1%	0.0M												97.2%	77%	151bp	151bp	45%	0.1M
NTC_0001_0001_A23YTGFLT4_1_2																																																		96.3%	97%	151bp	151bp	55%	0.1M
NTC_0001_0001_A23YTGFLT4_1_trimmed_1																																																		63.4%	64%	100bp	97bp	55%	0.0M	63.4%	64%	100bp	97bp	55%	0.0M
NTC_0001_0001_A23YTGFLT4_1_trimmed_2																																																		66.1%	72%	101bp	102bp	55%	0.0M	66.1%	72%	101bp	102bp	55%	0.0M
aih-tih-sc-01c779-R1_A23YTGFLT4_1	9999000000	273699329	520063753	0.9469	No	0.00%	541706048	0.01%	0.54%	248477124	0.92%	0.95%	0.06%	0.05%	229839749	115650134	15863055	6616623	372.55	168.00	0.70%	0.58%	0.97	0.000	119.7bp	111.0bp	0.06%	1.45%	0.45%	0.00%	0.12%	3.94%	0.65%	0.00%	0.01				54.4%	86.9%	95.0%	257.4M	30.4%	97.1%	61177.8Mb	541.7M	53.8%	98.8%	75.0%
aih-tih-sc-01c779-R1_A23YTGFLT4_1_1																																				119.7bp	97.5%	264.1M												81.9%	52%	151bp	151bp	18%	274.2M
aih-tih-sc-01c779-R1_A23YTGFLT4_1_2																																																		81.3%	53%	151bp	151bp	18%	274.2M
aih-tih-sc-01c779-R1_A23YTGFLT4_1_trimmed_1																																																		82.0%	53%	116bp	117bp	9%	270.9M	82.0%	53%	116bp	117bp	9%	270.9M
aih-tih-sc-01c779-R1_A23YTGFLT4_1_trimmed_2																																																		82.2%	53%	116bp	117bp	9%	270.9M	82.2%	53%	116bp	117bp	9%	270.9M
aih-tih-sc-01eb49-R1_A23YTGFLT4_1	9999000000	432893174	569173915	0.9202	No	0.00%	593180580	0.01%	0.47%	317061853	0.94%	0.97%	0.05%	0.03%	297300040	150586055	15117626	4808045	605.12	279.00	0.76%	0.65%	0.97	0.000	153.4bp	138.0bp	0.02%	0.69%	0.42%	0.00%	0.04%	2.08%	0.65%	0.00%	5.77				46.9%	87.8%	95.2%	282.3M	31.0%	97.2%	74925.6Mb	593.2M	56.1%	98.9%	50.7%
aih-tih-sc-01eb49-R1_A23YTGFLT4_1_1																																				153.4bp	97.5%	289.2M												84.7%	54%	151bp	151bp	18%	338.4M
aih-tih-sc-01eb49-R1_A23YTGFLT4_1_2																																																		83.8%	56%	151bp	151bp	18%	338.4M
aih-tih-sc-01eb49-R1_A23YTGFLT4_1_trimmed_1																																																		83.9%	55%	130bp	147bp	9%	296.6M	83.9%	55%	130bp	147bp	9%	296.6M
aih-tih-sc-01eb49-R1_A23YTGFLT4_1_trimmed_2																																																		83.9%	56%	130bp	147bp	9%	296.6M	83.9%	56%	130bp	147bp	9%	296.6M
aih-tih-sc-0754d2-R1_A23YTGFLT4_1	9999000000	10921206	54749590	0.8895	No	0.00%	59412030	0.01%	0.78%	13081316	0.86%	0.91%	0.10%	0.09%	11239206	5644138	1243323	540876	15.54	2.00	0.11%	0.03%	0.97	0.000	113.9bp	106.0bp	0.13%	0.69%	0.52%	0.00%	0.22%	1.80%	0.87%	0.00%	0.05				78.8%	75.3%	91.2%	27.1M	40.7%	96.9%	6297.5Mb	59.4M	62.3%	97.6%	81.7%
aih-tih-sc-0754d2-R1_A23YTGFLT4_1_1																																				113.9bp	96.6%	28.7M												82.3%	58%	151bp	151bp	27%	30.4M
aih-tih-sc-0754d2-R1_A23YTGFLT4_1_2																																																		80.5%	60%	151bp	151bp	36%	30.4M
aih-tih-sc-0754d2-R1_A23YTGFLT4_1_trimmed_1																																																		82.2%	62%	109bp	107bp	18%	29.7M	82.2%	62%	109bp	107bp	18%	29.7M
aih-tih-sc-0754d2-R1_A23YTGFLT4_1_trimmed_2																																																		81.6%	62%	110bp	107bp	9%	29.7M	81.6%	62%	110bp	107bp	9%	29.7M
aih-tih-sc-1274d4-R1_A23YTGFLT4_1	9999000000	295145678	504344129	0.9364	No	0.00%	521638756	0.01%	0.50%	261822191	0.93%	0.95%	0.06%	0.05%	242672127	122236680	16272533	6639877	413.54	218.00	0.74%	0.63%	0.97	0.000	125.9bp	115.0bp	0.02%	0.90%	0.42%	0.00%	0.04%	2.77%	0.65%	0.00%	0.02				50.1%	89.3%	95.8%	249.9M	27.3%	97.4%	59950.7Mb	521.6M	53.9%	98.8%	69.5%
aih-tih-sc-1274d4-R1_A23YTGFLT4_1_1																																				125.9bp	97.5%	254.2M												81.5%	52%	151bp	151bp	18%	264.0M
aih-tih-sc-1274d4-R1_A23YTGFLT4_1_2																																																		80.8%	53%	151bp	151bp	18%	264.0M
aih-tih-sc-1274d4-R1_A23YTGFLT4_1_trimmed_1																																																		81.5%	53%	118bp	122bp	9%	260.8M	81.5%	53%	118bp	122bp	9%	260.8M
aih-tih-sc-1274d4-R1_A23YTGFLT4_1_trimmed_2																																																		81.7%	54%	118bp	122bp	9%	260.8M	81.7%	54%	118bp	122bp	9%	260.8M
aih-tih-sc-13d441-R1_A23YTGFLT4_1	9999000000	314548651	499669605	0.8919	No	0.00%	515439328	0.01%	0.49%	264018488	0.93%	0.95%	0.06%	0.05%	245030330	123688044	16233142	6391392	440.86	236.00	0.76%	0.64%	0.97	0.000	132.4bp	121.0bp	0.03%	1.18%	0.33%	0.00%	0.06%	3.68%	0.53%	0.00%	0.02				49.0%	89.8%	96.0%	247.4M	29.9%	97.3%	61657.6Mb	515.4M	54.7%	99.0%	64.6%
aih-tih-sc-13d441-R1_A23YTGFLT4_1_1																																				132.4bp	97.5%	251.3M												81.7%	53%	151bp	151bp	18%	260.4M
aih-tih-sc-13d441-R1_A23YTGFLT4_1_2																																																		80.7%	54%	151bp	151bp	18%	260.4M
aih-tih-sc-13d441-R1_A23YTGFLT4_1_trimmed_1																																																		81.8%	54%	123bp	127bp	9%	257.7M	81.8%	54%	123bp	127bp	9%	257.7M
aih-tih-sc-13d441-R1_A23YTGFLT4_1_trimmed_2																																																		81.5%	54%	123bp	127bp	9%	257.7M	81.5%	54%	123bp	127bp	9%	257.7M
aih-tih-sc-150e73-R1_A23YTGFLT4_1	9999000000	250389008	563567811	0.9419	No	0.00%	591169684	0.01%	0.56%	262204331	0.93%	0.95%	0.06%	0.05%	242767914	122394990	15270873	5822155	430.99	168.00	0.72%	0.59%	0.97	0.000	133.8bp	122.0bp	0.02%	0.89%	0.48%	0.00%	0.04%	2.63%	0.71%	0.00%	3.74				56.0%	85.4%	94.5%	279.3M	34.5%	97.2%	70437.9Mb	591.2M	58.7%	98.5%	64.0%
aih-tih-sc-150e73-R1_A23YTGFLT4_1_1																																				133.8bp	97.4%	288.0M												85.6%	56%	151bp	151bp	18%	386.5M
aih-tih-sc-150e73-R1_A23YTGFLT4_1_2																																																		84.6%	57%	151bp	151bp	18%	386.5M
aih-tih-sc-150e73-R1_A23YTGFLT4_1_trimmed_1																																																		83.7%	58%	123bp	127bp	9%	295.6M	83.7%	58%	123bp	127bp	9%	295.6M
aih-tih-sc-150e73-R1_A23YTGFLT4_1_trimmed_2																																																		83.9%	58%	123bp	127bp	9%	295.6M	83.9%	58%	123bp	127bp	9%	295.6M
aih-tih-sc-184b05-R1_A23YTGFLT4_1	9999000000	269226969	567031548	0.9300	No	0.00%	592892210	0.01%	0.54%	270865975	0.93%	0.96%	0.06%	0.04%	251301351	126925739	15205991	5653685	463.65	188.00	0.73%	0.60%	0.97	0.000	141.9bp	131.0bp	0.02%	0.69%	0.36%	0.00%	0.04%	2.04%	0.55%	0.00%	5.34				54.7%	85.7%	94.8%	280.9M	36.8%	97.3%	73841.3Mb	592.9M	58.2%	98.8%	59.1%
aih-tih-sc-184b05-R1_A23YTGFLT4_1_1																																				141.9bp	97.7%	289.6M												87.2%	56%	151bp	151bp	18%	515.1M
aih-tih-sc-184b05-R1_A23YTGFLT4_1_2																																																		86.4%	57%	151bp	151bp	18%	515.1M
aih-tih-sc-184b05-R1_A23YTGFLT4_1_trimmed_1																																																		83.4%	57%	128bp	137bp	9%	296.4M	83.4%	57%	128bp	137bp	9%	296.4M
aih-tih-sc-184b05-R1_A23YTGFLT4_1_trimmed_2																																																		83.5%	58%	128bp	137bp	9%	296.4M	83.5%	58%	128bp	137bp	9%	296.4M
aih-tih-sc-242a7d-R1_A23YTGFLT4_1	9999000000	81639968	487114310	0.8812	No	0.00%	504863246	0.01%	0.84%	81044983	0.82%	0.86%	0.14%	0.14%	66081521	33185210	11547110	5339447	98.75	41.00	0.56%	0.28%	0.97	0.000	118.2bp	111.0bp	0.01%	0.73%	0.32%	0.00%	0.03%	2.49%	0.54%	0.00%	0.01				84.5%	87.3%	95.4%	240.9M	50.2%	97.3%	56934.7Mb	504.9M	57.2%	98.9%	76.7%
aih-tih-sc-242a7d-R1_A23YTGFLT4_1_1																																				118.2bp	97.2%	245.3M												88.3%	55%	151bp	151bp	18%	255.3M
aih-tih-sc-242a7d-R1_A23YTGFLT4_1_2																																																		87.5%	56%	151bp	151bp	18%	255.3M
aih-tih-sc-242a7d-R1_A23YTGFLT4_1_trimmed_1																																																		88.4%	57%	116bp	117bp	9%	252.4M	88.4%	57%	116bp	117bp	9%	252.4M
aih-tih-sc-242a7d-R1_A23YTGFLT4_1_trimmed_2																																																		88.4%	57%	116bp	117bp	9%	252.4M	88.4%	57%	116bp	117bp	9%	252.4M
aih-tih-sc-24ecfe-R1_A23YTGFLT4_1	9999000000	20048125	61712266	0.8734	No	0.00%	66063060	0.01%	0.78%	14504700	0.80%	0.86%	0.14%	0.14%	11608015	5806697	1959532	920701	13.49	3.00	0.10%	0.02%	0.97	0.000	95.5bp	91.0bp	0.04%	0.70%	0.56%	0.00%	0.07%	2.04%	0.81%	0.00%	5.07				79.2%	78.5%	92.2%	30.5M	29.0%	96.6%	6071.8Mb	66.1M	60.8%	96.9%	92.6%
aih-tih-sc-24ecfe-R1_A23YTGFLT4_1_1																																				95.5bp	94.4%	31.2M												78.7%	57%	151bp	151bp	36%	34.1M
aih-tih-sc-24ecfe-R1_A23YTGFLT4_1_2																																																		77.5%	59%	151bp	151bp	36%	34.1M
aih-tih-sc-24ecfe-R1_A23YTGFLT4_1_trimmed_1																																																		78.4%	60%	95bp	92bp	18%	33.0M	78.4%	60%	95bp	92bp	18%	33.0M
aih-tih-sc-24ecfe-R1_A23YTGFLT4_1_trimmed_2																																																		78.4%	61%	95bp	92bp	9%	33.0M	78.4%	61%	95bp	92bp	9%	33.0M
aih-tih-sc-28eb14-R1_A23YTGFLT4_1	9999000000	480464362	574195500	0.9120	No	0.00%	593428438	0.00%	0.44%	333412074	0.94%	0.96%	0.05%	0.04%	313623112	158627752	17067003	5975747	620.94	264.00	0.73%	0.63%	0.97	0.000	147.8bp	135.0bp	0.03%	0.73%	0.40%	0.00%	0.07%	2.14%	0.56%	0.00%	0.01				44.0%	89.0%	95.9%	284.6M	28.7%	97.3%	74803.4Mb	593.4M	54.8%	98.9%	54.1%
aih-tih-sc-28eb14-R1_A23YTGFLT4_1_1																																				147.8bp	97.8%	290.2M												84.2%	54%	151bp	151bp	18%	386.0M
aih-tih-sc-28eb14-R1_A23YTGFLT4_1_2																																																		83.5%	54%	151bp	151bp	18%	386.0M
aih-tih-sc-28eb14-R1_A23YTGFLT4_1_trimmed_1																																																		82.5%	54%	129bp	142bp	9%	296.7M	82.5%	54%	129bp	142bp	9%	296.7M
aih-tih-sc-28eb14-R1_A23YTGFLT4_1_trimmed_2																																																		82.6%	55%	130bp	142bp	9%	296.7M	82.6%	55%	130bp	142bp	9%	296.7M
aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1	9999000000	369278265	559467379	0.9219	Yes	0.00%	593521540	0.01%	0.49%	303803529	0.94%	0.96%	0.05%	0.04%	284312109	143313084	15897541	6126923	509.65	256.00	0.77%	0.65%	0.97	0.000	131.9bp	121.0bp	0.03%	0.57%	0.45%	0.00%	0.06%	1.85%	0.69%	0.00%	0.01				49.1%	83.7%	93.4%	277.2M	29.4%	97.3%	70587.3Mb	593.5M	55.4%	98.9%	66.3%
aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_1																																				131.9bp	97.9%	290.6M												83.1%	54%	151bp	151bp	18%	348.1M
aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_2																																																		82.6%	55%	151bp	151bp	18%	348.1M
aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_trimmed_1																																																		82.1%	55%	122bp	127bp	9%	296.8M	82.1%	55%	122bp	127bp	9%	296.8M
aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1_trimmed_2																																																		82.3%	55%	122bp	127bp	9%	296.8M	82.3%	55%	122bp	127bp	9%	296.8M
aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1	9999000000	498735649	573488181	0.8724	No	0.00%	592677972	0.01%	0.43%	339196023	0.94%	0.97%	0.05%	0.03%	318547428	161287112	17192585	5661980	647.31	308.00	0.75%	0.66%	0.97	0.000	153.7bp	139.0bp	0.01%	0.85%	0.46%	0.00%	0.03%	2.56%	0.66%	0.00%	6.01				43.0%	89.8%	95.9%	284.3M	28.9%	97.1%	75034.9Mb	592.7M	55.0%	98.8%	50.6%
aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_1																																				153.7bp	97.8%	289.9M												86.1%	54%	151bp	151bp	18%	414.4M
aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_2																																																		85.0%	55%	151bp	151bp	18%	414.4M
aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_trimmed_1																																																		84.1%	54%	130bp	147bp	9%	296.3M	84.1%	54%	130bp	147bp	9%	296.3M
aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_trimmed_2																																																		83.9%	55%	130bp	147bp	9%	296.3M	83.9%	55%	130bp	147bp	9%	296.3M
aih-tih-sc-318819-R1_A23YTGFLT4_1	9999000000	152688253	483987404	0.8861	No	0.00%	509610650	0.01%	0.70%	153018396	0.89%	0.91%	0.09%	0.09%	135708404	68152825	14161446	6393880	193.69	89.00	0.65%	0.48%	0.97	0.000	109.6bp	104.0bp	0.06%	0.95%	0.47%	0.00%	0.12%	2.82%	0.71%	0.00%	0.02				70.4%	84.0%	94.0%	239.5M	36.2%	97.4%	54346.7Mb	509.6M	56.2%	98.8%	83.8%
aih-tih-sc-318819-R1_A23YTGFLT4_1_1																																				109.6bp	97.4%	248.2M												83.5%	54%	151bp	151bp	18%	257.9M
aih-tih-sc-318819-R1_A23YTGFLT4_1_2																																																		82.8%	54%	151bp	151bp	27%	257.9M
aih-tih-sc-318819-R1_A23YTGFLT4_1_trimmed_1																																																		83.6%	56%	109bp	107bp	9%	254.8M	83.6%	56%	109bp	107bp	9%	254.8M
aih-tih-sc-318819-R1_A23YTGFLT4_1_trimmed_2																																																		83.7%	56%	110bp	107bp	9%	254.8M	83.7%	56%	110bp	107bp	9%	254.8M
aih-tih-sc-31d31f-R1_A23YTGFLT4_1	9999000000	80720596	351520520	0.9043	No	0.00%	385445184	0.01%	0.80%	78376661	0.81%	0.86%	0.15%	0.14%	63461614	31906014	11513623	5295431	96.04	37.00	0.54%	0.27%	0.97	0.000	120.1bp	112.0bp	0.04%	0.74%	0.28%	0.00%	0.10%	2.45%	0.49%	0.00%	0.19				80.4%	75.2%	90.4%	174.3M	46.3%	97.2%	43143.8Mb	385.4M	58.3%	98.5%	76.5%
aih-tih-sc-31d31f-R1_A23YTGFLT4_1_1																																				120.1bp	96.4%	185.7M												86.6%	56%	151bp	151bp	18%	195.7M
aih-tih-sc-31d31f-R1_A23YTGFLT4_1_2																																																		85.6%	57%	151bp	151bp	27%	195.7M
aih-tih-sc-31d31f-R1_A23YTGFLT4_1_trimmed_1																																																		86.7%	58%	115bp	117bp	9%	192.7M	86.7%	58%	115bp	117bp	9%	192.7M
aih-tih-sc-31d31f-R1_A23YTGFLT4_1_trimmed_2																																																		86.6%	58%	115bp	117bp	9%	192.7M	86.6%	58%	115bp	117bp	9%	192.7M
aih-tih-sc-3270ab-R1_A23YTGFLT4_1	9999000000	430445018	574307374	0.9529	No	0.00%	592127848	0.01%	0.46%	320821849	0.94%	0.97%	0.05%	0.03%	302493124	152678895	15285482	5355768	573.56	272.00	0.75%	0.65%	0.97	0.000	140.4bp	128.0bp	0.04%	0.86%	0.49%	0.00%	0.08%	2.67%	0.74%	0.00%	5.42				46.1%	90.2%	96.2%	284.7M	28.8%	97.1%	72345.4Mb	592.1M	56.6%	98.7%	58.5%
aih-tih-sc-3270ab-R1_A23YTGFLT4_1_1																																				140.4bp	98.0%	290.1M												85.3%	55%	151bp	151bp	18%	452.8M
aih-tih-sc-3270ab-R1_A23YTGFLT4_1_2																																																		84.4%	56%	151bp	151bp	18%	452.8M
aih-tih-sc-3270ab-R1_A23YTGFLT4_1_trimmed_1																																																		82.3%	56%	126bp	137bp	9%	296.1M	82.3%	56%	126bp	137bp	9%	296.1M
aih-tih-sc-3270ab-R1_A23YTGFLT4_1_trimmed_2																																																		82.6%	56%	126bp	137bp	9%	296.1M	82.6%	56%	126bp	137bp	9%	296.1M
aih-tih-sc-3282fd-R1_A23YTGFLT4_1	9999000000	193072018	469309781	0.8816	No	0.00%	486927850	0.01%	0.60%	194265269	0.91%	0.94%	0.07%	0.06%	176996277	89072564	14333387	6151410	271.56	135.00	0.70%	0.55%	0.97	0.000	116.5bp	109.0bp	0.05%	1.09%	0.53%	0.00%	0.10%	3.13%	0.76%	0.00%	6.03				60.5%	87.5%	95.4%	232.3M	32.5%	97.4%	53838.3Mb	486.9M	55.0%	98.9%	78.6%
aih-tih-sc-3282fd-R1_A23YTGFLT4_1_1																																				116.5bp	97.4%	237.1M												82.1%	53%	151bp	151bp	18%	246.1M
aih-tih-sc-3282fd-R1_A23YTGFLT4_1_2																																																		81.1%	54%	151bp	151bp	18%	246.1M
aih-tih-sc-3282fd-R1_A23YTGFLT4_1_trimmed_1																																																		82.2%	54%	113bp	112bp	9%	243.5M	82.2%	54%	113bp	112bp	9%	243.5M
aih-tih-sc-3282fd-R1_A23YTGFLT4_1_trimmed_2																																																		81.8%	55%	114bp	112bp	9%	243.5M	81.8%	55%	114bp	112bp	9%	243.5M
aih-tih-sc-3377ac-R1_A23YTGFLT4_1	9999000000	193475869	574765493	0.8769	Yes	0.00%	593533050	0.01%	0.63%	218070723	0.92%	0.94%	0.06%	0.06%	200771163	101036271	14166286	5942744	330.46	150.00	0.71%	0.57%	0.97	0.000	121.9bp	114.0bp	0.02%	0.68%	0.31%	0.00%	0.05%	2.25%	0.52%	0.00%	0.11				63.6%	89.0%	96.0%	284.8M	37.6%	97.3%	68121.0Mb	593.5M	57.2%	98.9%	72.9%
aih-tih-sc-3377ac-R1_A23YTGFLT4_1_1																																				121.9bp	97.9%	290.5M												86.0%	55%	151bp	151bp	18%	355.2M
aih-tih-sc-3377ac-R1_A23YTGFLT4_1_2																																																		85.0%	56%	151bp	151bp	18%	355.2M
aih-tih-sc-3377ac-R1_A23YTGFLT4_1_trimmed_1																																																		84.9%	57%	118bp	122bp	18%	296.8M	84.9%	57%	118bp	122bp	18%	296.8M
aih-tih-sc-3377ac-R1_A23YTGFLT4_1_trimmed_2																																																		84.7%	57%	118bp	122bp	9%	296.8M	84.7%	57%	118bp	122bp	9%	296.8M
aih-tih-sc-377071-R1_A23YTGFLT4_1	9999000000	470180691	573719878	0.9451	No	0.00%	593168166	0.01%	0.44%	333243842	0.93%	0.96%	0.06%	0.04%	311158284	157473728	18822305	6642408	616.95	296.00	0.76%	0.65%	0.97	0.000	150.6bp	138.0bp	0.02%	0.51%	0.34%	0.00%	0.05%	1.54%	0.50%	0.00%	0.02				44.1%	89.6%	95.8%	284.2M	29.5%	97.3%	74803.3Mb	593.2M	54.9%	98.9%	52.9%
aih-tih-sc-377071-R1_A23YTGFLT4_1_1																																				150.6bp	97.3%	288.6M												84.1%	53%	151bp	151bp	18%	328.8M
aih-tih-sc-377071-R1_A23YTGFLT4_1_2																																																		83.4%	55%	151bp	151bp	18%	328.8M
aih-tih-sc-377071-R1_A23YTGFLT4_1_trimmed_1																																																		83.5%	54%	130bp	147bp	9%	296.6M	83.5%	54%	130bp	147bp	9%	296.6M
aih-tih-sc-377071-R1_A23YTGFLT4_1_trimmed_2																																																		83.7%	55%	130bp	147bp	9%	296.6M	83.7%	55%	130bp	147bp	9%	296.6M
aih-tih-sc-3abdf4-R1_A23YTGFLT4_1	9999000000	176150060	459254520	0.8622	No	0.00%	479167942	0.01%	0.62%	181105571	0.90%	0.92%	0.09%	0.08%	162433470	81802702	15413922	6640167	269.11	112.00	0.68%	0.52%	0.97	0.000	127.7bp	119.0bp	0.02%	0.69%	0.31%	0.00%	0.05%	2.12%	0.47%	0.00%	0.04				62.6%	85.7%	95.0%	227.7M	38.9%	97.2%	55924.4Mb	479.2M	57.3%	98.7%	70.6%
aih-tih-sc-3abdf4-R1_A23YTGFLT4_1_1																																				127.7bp	96.8%	231.9M												82.4%	55%	151bp	151bp	18%	242.6M
aih-tih-sc-3abdf4-R1_A23YTGFLT4_1_2																																																		81.6%	56%	151bp	151bp	27%	242.6M
aih-tih-sc-3abdf4-R1_A23YTGFLT4_1_trimmed_1																																																		82.5%	57%	120bp	122bp	9%	239.6M	82.5%	57%	120bp	122bp	9%	239.6M
aih-tih-sc-3abdf4-R1_A23YTGFLT4_1_trimmed_2																																																		82.5%	57%	120bp	122bp	9%	239.6M	82.5%	57%	120bp	122bp	9%	239.6M
aih-tih-sc-3b436b-R1_A23YTGFLT4_1	9999000000	354250145	248531713	0.9662	No	0.00%	257622240	0.01%	0.32%	175589868	0.94%	0.96%	0.05%	0.04%	165102422	83468204	8765657	3101799	319.95	155.00	0.71%	0.57%	0.98	0.000	146.0bp	132.0bp	0.03%	1.53%	0.58%	0.00%	0.06%	4.49%	0.82%	0.00%	6.46				32.1%	89.0%	95.5%	123.1M	19.4%	97.4%	31966.8Mb	257.6M	53.2%	98.9%	56.2%
aih-tih-sc-3b436b-R1_A23YTGFLT4_1_1																																				146.0bp	97.7%	125.9M												76.5%	52%	151bp	151bp	18%	130.2M
aih-tih-sc-3b436b-R1_A23YTGFLT4_1_2																																																		76.1%	53%	151bp	151bp	18%	130.2M
aih-tih-sc-3b436b-R1_A23YTGFLT4_1_trimmed_1																																																		76.5%	52%	127bp	142bp	9%	128.8M	76.5%	52%	127bp	142bp	9%	128.8M
aih-tih-sc-3b436b-R1_A23YTGFLT4_1_trimmed_2																																																		76.8%	53%	127bp	142bp	9%	128.8M	76.8%	53%	127bp	142bp	9%	128.8M
aih-tih-sc-45523c-R1_A23YTGFLT4_1	9999000000	170069409	464703408	0.8401	No	0.00%	482225620	0.01%	0.65%	166879961	0.90%	0.93%	0.08%	0.07%	149988900	75549817	13876628	5975109	255.65	120.00	0.70%	0.54%	0.97	0.000	131.0bp	122.0bp	0.01%	0.58%	0.28%	0.00%	0.03%	2.02%	0.47%	0.00%	0.02				65.8%	88.2%	95.5%	230.3M	42.8%	97.3%	57907.4Mb	482.2M	57.4%	98.9%	67.1%
aih-tih-sc-45523c-R1_A23YTGFLT4_1_1																																				131.0bp	97.3%	234.6M												83.0%	56%	151bp	151bp	18%	243.7M
aih-tih-sc-45523c-R1_A23YTGFLT4_1_2																																																		82.4%	56%	151bp	151bp	18%	243.7M
aih-tih-sc-45523c-R1_A23YTGFLT4_1_trimmed_1																																																		83.0%	57%	123bp	127bp	9%	241.1M	83.0%	57%	123bp	127bp	9%	241.1M
aih-tih-sc-45523c-R1_A23YTGFLT4_1_trimmed_2																																																		83.2%	57%	123bp	127bp	9%	241.1M	83.2%	57%	123bp	127bp	9%	241.1M
aih-tih-sc-49909d-R1_A23YTGFLT4_1	9999000000	218683351	509196598	0.9213	No	0.00%	531275772	0.01%	0.57%	227183241	0.91%	0.94%	0.07%	0.06%	207770336	104713669	15364161	6314919	351.34	161.00	0.71%	0.58%	0.97	0.000	126.5bp	117.0bp	0.03%	0.84%	0.39%	0.00%	0.07%	2.52%	0.59%	0.00%	5.85				57.7%	85.3%	94.9%	252.2M	33.8%	97.3%	61751.0Mb	531.3M	56.4%	98.8%	70.6%
aih-tih-sc-49909d-R1_A23YTGFLT4_1_1																																				126.5bp	97.1%	257.9M												82.6%	54%	151bp	151bp	18%	268.8M
aih-tih-sc-49909d-R1_A23YTGFLT4_1_2																																																		81.8%	56%	151bp	151bp	18%	268.8M
aih-tih-sc-49909d-R1_A23YTGFLT4_1_trimmed_1																																																		82.7%	56%	119bp	122bp	9%	265.6M	82.7%	56%	119bp	122bp	9%	265.6M
aih-tih-sc-49909d-R1_A23YTGFLT4_1_trimmed_2																																																		82.6%	56%	120bp	122bp	9%	265.6M	82.6%	56%	120bp	122bp	9%	265.6M
aih-tih-sc-4bcd26-R1_A23YTGFLT4_1	9999000000	202469147	375712936	0.9431	No	0.00%	388420350	0.01%	0.53%	182988525	0.91%	0.93%	0.08%	0.07%	166427625	83651461	14494007	6390994	266.32	133.00	0.71%	0.55%	0.97	0.000	121.5bp	113.0bp	0.02%	1.18%	0.36%	0.00%	0.05%	3.65%	0.56%	0.00%	0.05				53.2%	88.6%	95.7%	185.8M	29.9%	97.3%	44172.9Mb	388.4M	53.8%	98.7%	75.1%
aih-tih-sc-4bcd26-R1_A23YTGFLT4_1_1																																				121.5bp	96.8%	188.1M												79.0%	53%	151bp	151bp	18%	196.7M
aih-tih-sc-4bcd26-R1_A23YTGFLT4_1_2																																																		78.3%	54%	151bp	151bp	18%	196.7M
aih-tih-sc-4bcd26-R1_A23YTGFLT4_1_trimmed_1																																																		79.1%	53%	117bp	117bp	9%	194.2M	79.1%	53%	117bp	117bp	9%	194.2M
aih-tih-sc-4bcd26-R1_A23YTGFLT4_1_trimmed_2																																																		79.3%	53%	117bp	117bp	9%	194.2M	79.3%	53%	117bp	117bp	9%	194.2M
aih-tih-sc-538ae3-R1_A23YTGFLT4_1	9999000000	490111858	562367782	0.9179	No	0.00%	593710370	0.01%	0.44%	335294949	0.94%	0.96%	0.05%	0.04%	315291097	159300213	16836533	6086682	601.06	293.00	0.74%	0.65%	0.97	0.000	141.4bp	128.0bp	0.05%	1.18%	0.61%	0.00%	0.11%	3.54%	0.90%	0.00%	0.01				43.8%	84.7%	94.0%	278.9M	27.5%	97.3%	72869.9Mb	593.7M	54.7%	99.0%	58.9%
aih-tih-sc-538ae3-R1_A23YTGFLT4_1_1																																				141.4bp	97.7%	290.0M												84.3%	53%	151bp	151bp	18%	409.8M
aih-tih-sc-538ae3-R1_A23YTGFLT4_1_2																																																		83.9%	54%	151bp	151bp	18%	409.8M
aih-tih-sc-538ae3-R1_A23YTGFLT4_1_trimmed_1																																																		82.2%	54%	126bp	137bp	9%	296.9M	82.2%	54%	126bp	137bp	9%	296.9M
aih-tih-sc-538ae3-R1_A23YTGFLT4_1_trimmed_2																																																		82.6%	54%	126bp	137bp	9%	296.9M	82.6%	54%	126bp	137bp	9%	296.9M
aih-tih-sc-5519ab-R1_A23YTGFLT4_1	9999000000	230501419	571734560	0.8998	No	0.00%	593086390	0.01%	0.58%	246345117	0.92%	0.94%	0.07%	0.06%	225895130	113902175	17382501	7145497	393.59	143.00	0.68%	0.55%	0.97	0.000	129.4bp	119.0bp	0.01%	0.84%	0.46%	0.00%	0.03%	2.63%	0.71%	0.00%	6.14				58.8%	87.9%	95.6%	283.6M	33.9%	97.3%	69723.8Mb	593.1M	57.2%	98.8%	67.5%
aih-tih-sc-5519ab-R1_A23YTGFLT4_1_1																																				129.4bp	97.2%	288.3M												83.6%	55%	151bp	151bp	18%	350.5M
aih-tih-sc-5519ab-R1_A23YTGFLT4_1_2																																																		83.1%	56%	151bp	151bp	18%	350.5M
aih-tih-sc-5519ab-R1_A23YTGFLT4_1_trimmed_1																																																		82.5%	56%	121bp	127bp	9%	296.5M	82.5%	56%	121bp	127bp	9%	296.5M
aih-tih-sc-5519ab-R1_A23YTGFLT4_1_trimmed_2																																																		82.8%	57%	121bp	127bp	9%	296.5M	82.8%	57%	121bp	127bp	9%	296.5M
aih-tih-sc-56cab8-R1_A23YTGFLT4_1	9999000000	373745148	569194240	0.9625	No	0.00%	593048614	0.01%	0.48%	309993584	0.93%	0.96%	0.05%	0.04%	289730712	146256902	16867060	6357581	538.16	223.00	0.71%	0.60%	0.97	0.000	139.0bp	127.0bp	0.03%	1.57%	0.49%	0.00%	0.07%	4.44%	0.71%	0.00%	2.96				48.0%	87.7%	95.2%	282.2M	29.6%	97.3%	72637.8Mb	593.0M	55.5%	98.8%	60.8%
aih-tih-sc-56cab8-R1_A23YTGFLT4_1_1																																				139.0bp	97.6%	289.5M												83.2%	54%	151bp	151bp	18%	356.7M
aih-tih-sc-56cab8-R1_A23YTGFLT4_1_2																																																		82.2%	55%	151bp	151bp	18%	356.7M
aih-tih-sc-56cab8-R1_A23YTGFLT4_1_trimmed_1																																																		82.0%	55%	126bp	132bp	9%	296.5M	82.0%	55%	126bp	132bp	9%	296.5M
aih-tih-sc-56cab8-R1_A23YTGFLT4_1_trimmed_2																																																		81.8%	55%	126bp	137bp	9%	296.5M	81.8%	55%	126bp	137bp	9%	296.5M
aih-tih-sc-573e21-R1_A23YTGFLT4_1	9999000000	211082983	572814787	0.9380	No	0.00%	591761794	0.01%	0.60%	236097926	0.93%	0.96%	0.06%	0.04%	219964106	110961122	13171163	4865270	404.60	155.00	0.71%	0.57%	0.97	0.000	138.5bp	127.0bp	0.01%	0.58%	0.37%	0.00%	0.03%	1.83%	0.57%	0.00%	5.80				60.4%	88.7%	95.9%	283.8M	39.7%	97.2%	72179.0Mb	591.8M	58.3%	98.6%	60.0%
aih-tih-sc-573e21-R1_A23YTGFLT4_1_1																																				138.5bp	98.0%	289.9M												86.4%	57%	151bp	151bp	18%	324.7M
aih-tih-sc-573e21-R1_A23YTGFLT4_1_2																																																		85.6%	57%	151bp	151bp	18%	324.7M
aih-tih-sc-573e21-R1_A23YTGFLT4_1_trimmed_1																																																		85.9%	58%	125bp	137bp	9%	295.9M	85.9%	58%	125bp	137bp	9%	295.9M
aih-tih-sc-573e21-R1_A23YTGFLT4_1_trimmed_2																																																		86.1%	58%	126bp	137bp	9%	295.9M	86.1%	58%	126bp	137bp	9%	295.9M
aih-tih-sc-598a93-R1_A23YTGFLT4_1	9999000000	31456482	113915265	0.8580	No	0.00%	121486570	0.01%	0.79%	26038232	0.80%	0.85%	0.15%	0.15%	20853436	10459849	3957632	1860304	29.08	11.00	0.26%	0.06%	0.97	0.000	112.6bp	106.0bp	0.02%	0.36%	0.12%	0.00%	0.05%	1.23%	0.23%	0.00%	0.02				79.4%	78.3%	92.7%	56.3M	42.0%	97.2%	12982.1Mb	121.5M	59.5%	98.4%	82.2%
aih-tih-sc-598a93-R1_A23YTGFLT4_1_1																																				112.6bp	96.0%	58.3M												82.0%	57%	151bp	151bp	18%	61.7M
aih-tih-sc-598a93-R1_A23YTGFLT4_1_2																																																		80.6%	58%	151bp	151bp	27%	61.7M
aih-tih-sc-598a93-R1_A23YTGFLT4_1_trimmed_1																																																		82.0%	59%	110bp	107bp	9%	60.7M	82.0%	59%	110bp	107bp	9%	60.7M
aih-tih-sc-598a93-R1_A23YTGFLT4_1_trimmed_2																																																		81.4%	59%	110bp	107bp	9%	60.7M	81.4%	59%	110bp	107bp	9%	60.7M
aih-tih-sc-5b8b4a-R1_A23YTGFLT4_1	9999000000	317597713	572007190	0.9480	No	0.00%	593009460	0.01%	0.54%	274266979	0.92%	0.95%	0.06%	0.05%	253381102	127768391	17533368	7069001	437.29	210.00	0.72%	0.62%	0.97	0.000	129.3bp	119.0bp	0.05%	1.11%	0.39%	0.00%	0.09%	3.19%	0.56%	0.00%	6.29				54.1%	88.6%	95.5%	283.3M	32.0%	97.1%	69708.6Mb	593.0M	55.3%	98.8%	68.3%
aih-tih-sc-5b8b4a-R1_A23YTGFLT4_1_1																																				129.3bp	97.3%	288.5M												83.1%	54%	151bp	151bp	18%	344.5M
aih-tih-sc-5b8b4a-R1_A23YTGFLT4_1_2																																																		82.6%	54%	151bp	151bp	18%	344.5M
aih-tih-sc-5b8b4a-R1_A23YTGFLT4_1_trimmed_1																																																		82.1%	55%	121bp	127bp	9%	296.5M	82.1%	55%	121bp	127bp	9%	296.5M
aih-tih-sc-5b8b4a-R1_A23YTGFLT4_1_trimmed_2																																																		82.4%	55%	121bp	127bp	9%	296.5M	82.4%	55%	121bp	127bp	9%	296.5M
aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1	9999000000	498188471	553544638	0.9476	No	0.00%	570485302	0.00%	0.42%	330788262	0.94%	0.96%	0.06%	0.04%	309504933	156490819	18632079	6903788	595.60	308.00	0.76%	0.66%	0.97	0.000	142.8bp	130.0bp	0.03%	0.92%	0.37%	0.00%	0.06%	2.85%	0.57%	0.00%	0.01				42.2%	90.4%	96.1%	274.2M	27.3%	97.4%	70896.0Mb	570.5M	53.7%	99.0%	57.6%
aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_1																																				142.8bp	97.6%	278.3M												82.0%	52%	151bp	151bp	18%	288.0M
aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_2																																																		81.1%	53%	151bp	151bp	18%	288.0M
aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_trimmed_1																																																		82.1%	53%	128bp	137bp	9%	285.2M	82.1%	53%	128bp	137bp	9%	285.2M
aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1_trimmed_2																																																		81.9%	53%	128bp	137bp	9%	285.2M	81.9%	53%	128bp	137bp	9%	285.2M
aih-tih-sc-607ec3-R1_A23YTGFLT4_1	9999000000	106402110	338657862	0.9537	No	0.00%	367547450	0.01%	0.66%	123795294	0.90%	0.93%	0.08%	0.07%	111728964	56137142	9589835	4161138	173.92	66.00	0.62%	0.42%	0.97	0.000	118.9bp	111.0bp	0.06%	1.10%	0.46%	0.00%	0.12%	3.36%	0.73%	0.00%	0.03				66.8%	77.8%	91.3%	167.8M	38.3%	97.2%	41200.3Mb	367.5M	57.9%	98.6%	76.7%
aih-tih-sc-607ec3-R1_A23YTGFLT4_1_1																																				118.9bp	97.3%	178.9M												82.8%	55%	151bp	151bp	27%	186.3M
aih-tih-sc-607ec3-R1_A23YTGFLT4_1_2																																																		81.9%	56%	151bp	151bp	18%	186.3M
aih-tih-sc-607ec3-R1_A23YTGFLT4_1_trimmed_1																																																		82.8%	57%	115bp	117bp	18%	183.8M	82.8%	57%	115bp	117bp	18%	183.8M
aih-tih-sc-607ec3-R1_A23YTGFLT4_1_trimmed_2																																																		82.9%	58%	115bp	117bp	9%	183.8M	82.9%	58%	115bp	117bp	9%	183.8M
aih-tih-sc-64cd51-R1_A23YTGFLT4_1	9999000000	351897516	572976387	0.9405	No	0.00%	593263512	0.01%	0.49%	300019917	0.93%	0.95%	0.06%	0.05%	278962287	140853932	17769230	6831830	497.70	239.00	0.76%	0.64%	0.97	0.000	132.0bp	122.0bp	0.05%	1.11%	0.51%	0.00%	0.10%	3.12%	0.68%	0.00%	0.01				49.7%	89.0%	95.7%	283.9M	26.9%	97.3%	70711.5Mb	593.3M	55.3%	98.9%	65.7%
aih-tih-sc-64cd51-R1_A23YTGFLT4_1_1																																				132.0bp	97.5%	289.3M												82.4%	54%	151bp	151bp	18%	316.8M
aih-tih-sc-64cd51-R1_A23YTGFLT4_1_2																																																		81.8%	55%	151bp	151bp	18%	316.8M
aih-tih-sc-64cd51-R1_A23YTGFLT4_1_trimmed_1																																																		82.0%	55%	122bp	127bp	9%	296.6M	82.0%	55%	122bp	127bp	9%	296.6M
aih-tih-sc-64cd51-R1_A23YTGFLT4_1_trimmed_2																																																		82.2%	55%	123bp	127bp	9%	296.6M	82.2%	55%	123bp	127bp	9%	296.6M
aih-tih-sc-64fc89-R1_A23YTGFLT4_1	9999000000	382297880	575237394	0.9301	No	0.00%	593662950	0.01%	0.48%	308484804	0.94%	0.96%	0.05%	0.04%	288603703	145765651	16735043	6264579	537.27	250.00	0.74%	0.63%	0.97	0.000	138.8bp	128.0bp	0.01%	1.71%	0.54%	0.00%	0.01%	5.12%	0.84%	0.00%	0.01				48.3%	89.6%	96.0%	284.9M	31.4%	97.3%	73027.0Mb	593.7M	54.9%	98.9%	60.9%
aih-tih-sc-64fc89-R1_A23YTGFLT4_1_1																																				138.8bp	97.8%	290.3M												83.8%	53%	151bp	151bp	18%	347.3M
aih-tih-sc-64fc89-R1_A23YTGFLT4_1_2																																																		82.9%	54%	151bp	151bp	18%	347.3M
aih-tih-sc-64fc89-R1_A23YTGFLT4_1_trimmed_1																																																		82.8%	54%	126bp	137bp	9%	296.8M	82.8%	54%	126bp	137bp	9%	296.8M
aih-tih-sc-64fc89-R1_A23YTGFLT4_1_trimmed_2																																																		82.7%	55%	126bp	137bp	9%	296.8M	82.7%	55%	126bp	137bp	9%	296.8M
aih-tih-sc-656590-R1_A23YTGFLT4_1	9999000000	387285213	516859552	0.9057	No	0.00%	535391458	0.01%	0.45%	292981267	0.94%	0.96%	0.05%	0.04%	274647239	138620400	14907537	5497061	514.32	218.00	0.72%	0.61%	0.97	0.000	139.2bp	128.0bp	0.03%	0.87%	0.46%	0.00%	0.05%	2.58%	0.68%	0.00%	3.61				45.6%	89.0%	95.7%	256.2M	28.3%	97.3%	65505.4Mb	535.4M	55.5%	98.9%	61.0%
aih-tih-sc-656590-R1_A23YTGFLT4_1_1																																				139.2bp	97.6%	261.4M												82.0%	54%	151bp	151bp	18%	270.7M
aih-tih-sc-656590-R1_A23YTGFLT4_1_2																																																		81.7%	55%	151bp	151bp	18%	270.7M
aih-tih-sc-656590-R1_A23YTGFLT4_1_trimmed_1																																																		82.1%	55%	126bp	132bp	9%	267.7M	82.1%	55%	126bp	132bp	9%	267.7M
aih-tih-sc-656590-R1_A23YTGFLT4_1_trimmed_2																																																		82.5%	55%	126bp	132bp	9%	267.7M	82.5%	55%	126bp	132bp	9%	267.7M
aih-tih-sc-67e6e5-R1_A23YTGFLT4_1	9999000000	159122817	566969786	0.9094	No	0.00%	591543294	0.01%	0.67%	194989104	0.90%	0.93%	0.08%	0.07%	175404551	88540827	15514417	6298150	299.66	126.00	0.71%	0.55%	0.97	0.000	132.5bp	121.0bp	0.01%	0.44%	0.30%	0.00%	0.01%	1.36%	0.48%	0.00%	6.09				67.5%	85.6%	95.0%	281.1M	41.4%	97.1%	69732.6Mb	591.5M	59.3%	98.6%	66.3%
aih-tih-sc-67e6e5-R1_A23YTGFLT4_1_1																																				132.5bp	96.9%	286.7M												87.1%	56%	151bp	151bp	18%	374.0M
aih-tih-sc-67e6e5-R1_A23YTGFLT4_1_2																																																		85.6%	58%	151bp	151bp	18%	374.0M
aih-tih-sc-67e6e5-R1_A23YTGFLT4_1_trimmed_1																																																		85.6%	59%	121bp	127bp	9%	295.8M	85.6%	59%	121bp	127bp	9%	295.8M
aih-tih-sc-67e6e5-R1_A23YTGFLT4_1_trimmed_2																																																		85.1%	59%	121bp	127bp	9%	295.8M	85.1%	59%	121bp	127bp	9%	295.8M
aih-tih-sc-69be7e-R1_A23YTGFLT4_1	9990000000	275096741	571637230	0.8825	Yes	0.00%	592374120	0.01%	0.54%	272241509	0.92%	0.95%	0.07%	0.05%	251046644	126759962	17828023	6953143	452.24	181.00	0.73%	0.60%	0.97	0.000	137.3bp	126.0bp	0.02%	0.93%	0.32%	0.00%	0.03%	2.72%	0.50%	0.00%	6.15				54.4%	88.2%	95.7%	283.3M	35.1%	97.0%	71708.8Mb	592.4M	58.8%	98.7%	62.1%
aih-tih-sc-69be7e-R1_A23YTGFLT4_1_1																																				137.3bp	97.4%	288.4M												83.9%	57%	151bp	151bp	18%	309.8M
aih-tih-sc-69be7e-R1_A23YTGFLT4_1_2																																																		83.4%	58%	151bp	151bp	18%	309.8M
aih-tih-sc-69be7e-R1_A23YTGFLT4_1_trimmed_1																																																		83.8%	58%	125bp	132bp	9%	296.2M	83.8%	58%	125bp	132bp	9%	296.2M
aih-tih-sc-69be7e-R1_A23YTGFLT4_1_trimmed_2																																																		84.1%	59%	125bp	132bp	9%	296.2M	84.1%	59%	125bp	132bp	9%	296.2M
aih-tih-sc-6c4b48-R1_A23YTGFLT4_1	9999000000	574757938	574305444	0.9140	No	0.00%	593375942	0.00%	0.41%	350239699	0.94%	0.96%	0.05%	0.04%	328805330	166907901	18837309	6059250	692.18	264.00	0.70%	0.61%	0.97	0.000	161.5bp	147.0bp	0.02%	0.92%	0.44%	0.00%	0.05%	2.83%	0.65%	0.00%	5.91				41.2%	90.1%	95.9%	284.6M	28.2%	97.3%	76989.7Mb	593.4M	54.8%	98.9%	46.0%
aih-tih-sc-6c4b48-R1_A23YTGFLT4_1_1																																				161.5bp	97.6%	289.7M												85.3%	53%	151bp	151bp	18%	463.9M
aih-tih-sc-6c4b48-R1_A23YTGFLT4_1_2																																																		84.8%	55%	151bp	151bp	18%	463.9M
aih-tih-sc-6c4b48-R1_A23YTGFLT4_1_trimmed_1																																																		82.6%	54%	133bp	151bp	9%	296.7M	82.6%	54%	133bp	151bp	9%	296.7M
aih-tih-sc-6c4b48-R1_A23YTGFLT4_1_trimmed_2																																																		83.0%	55%	133bp	151bp	9%	296.7M	83.0%	55%	133bp	151bp	9%	296.7M
aih-tih-sc-6cc13c-R1_A23YTGFLT4_1	9999000000	368932334	574075198	0.8820	No	0.00%	593770278	0.01%	0.52%	284497658	0.93%	0.95%	0.06%	0.05%	264259937	133262026	17146047	6870157	463.01	212.00	0.75%	0.62%	0.97	0.000	128.9bp	119.0bp	0.02%	1.59%	0.45%	0.00%	0.03%	4.51%	0.70%	0.00%	0.02				52.4%	88.4%	95.7%	284.1M	31.4%	97.4%	69893.4Mb	593.8M	55.7%	99.0%	68.3%
aih-tih-sc-6cc13c-R1_A23YTGFLT4_1_1																																				128.9bp	97.7%	290.1M												83.9%	54%	151bp	151bp	18%	407.1M
aih-tih-sc-6cc13c-R1_A23YTGFLT4_1_2																																																		83.0%	55%	151bp	151bp	18%	407.1M
aih-tih-sc-6cc13c-R1_A23YTGFLT4_1_trimmed_1																																																		81.8%	55%	121bp	127bp	9%	296.9M	81.8%	55%	121bp	127bp	9%	296.9M
aih-tih-sc-6cc13c-R1_A23YTGFLT4_1_trimmed_2																																																		81.6%	55%	121bp	127bp	9%	296.9M	81.6%	55%	121bp	127bp	9%	296.9M
aih-tih-sc-6d9e6f-R1_A23YTGFLT4_1	9999000000	384987376	574621606	0.9159	No	0.00%	592794558	0.00%	0.47%	314694859	0.93%	0.95%	0.06%	0.05%	293010372	147922260	18748607	7238082	536.37	295.00	0.77%	0.67%	0.97	0.000	136.6bp	125.0bp	0.06%	1.19%	0.42%	0.00%	0.12%	3.58%	0.66%	0.00%	0.05				47.1%	90.0%	96.0%	284.5M	29.3%	97.3%	71503.2Mb	592.8M	54.1%	98.8%	62.0%
aih-tih-sc-6d9e6f-R1_A23YTGFLT4_1_1																																				136.6bp	97.5%	289.0M												83.0%	53%	151bp	151bp	18%	345.5M
aih-tih-sc-6d9e6f-R1_A23YTGFLT4_1_2																																																		82.4%	54%	151bp	151bp	18%	345.5M
aih-tih-sc-6d9e6f-R1_A23YTGFLT4_1_trimmed_1																																																		81.9%	53%	124bp	132bp	9%	296.4M	81.9%	53%	124bp	132bp	9%	296.4M
aih-tih-sc-6d9e6f-R1_A23YTGFLT4_1_trimmed_2																																																		82.3%	54%	124bp	132bp	9%	296.4M	82.3%	54%	124bp	132bp	9%	296.4M
aih-tih-sc-714733-R1_A23YTGFLT4_1	9999000000	361021493	573069904	0.9120	No	0.00%	593455294	0.01%	0.49%	303947808	0.93%	0.96%	0.06%	0.04%	283663121	143395695	16856126	6187115	536.05	261.00	0.76%	0.65%	0.97	0.000	140.6bp	129.0bp	0.07%	0.76%	0.40%	0.00%	0.15%	2.38%	0.57%	0.00%	0.01				49.1%	88.9%	95.6%	283.7M	31.5%	97.4%	73376.8Mb	593.5M	55.0%	98.9%	58.4%
aih-tih-sc-714733-R1_A23YTGFLT4_1_1																																				140.6bp	97.7%	289.8M												84.0%	54%	151bp	151bp	18%	347.2M
aih-tih-sc-714733-R1_A23YTGFLT4_1_2																																																		83.6%	54%	151bp	151bp	18%	347.2M
aih-tih-sc-714733-R1_A23YTGFLT4_1_trimmed_1																																																		82.9%	54%	127bp	137bp	9%	296.7M	82.9%	54%	127bp	137bp	9%	296.7M
aih-tih-sc-714733-R1_A23YTGFLT4_1_trimmed_2																																																		83.4%	55%	127bp	137bp	9%	296.7M	83.4%	55%	127bp	137bp	9%	296.7M
aih-tih-sc-776623-R1_A23YTGFLT4_1	9999000000	193366973	572769971	0.8997	No	0.00%	593603618	0.01%	0.64%	211966757	0.91%	0.95%	0.07%	0.05%	193796793	98001962	14728983	5656628	348.81	167.00	0.73%	0.59%	0.97	0.000	134.7bp	122.0bp	0.02%	1.03%	0.32%	0.00%	0.05%	3.29%	0.53%	0.00%	0.01				64.7%	87.6%	95.5%	283.6M	40.2%	97.2%	71424.1Mb	593.6M	56.3%	98.9%	62.7%
aih-tih-sc-776623-R1_A23YTGFLT4_1_1																																				134.7bp	97.8%	290.4M												86.6%	54%	151bp	151bp	18%	350.8M
aih-tih-sc-776623-R1_A23YTGFLT4_1_2																																																		85.1%	55%	151bp	151bp	27%	350.8M
aih-tih-sc-776623-R1_A23YTGFLT4_1_trimmed_1																																																		85.6%	56%	124bp	132bp	9%	296.8M	85.6%	56%	124bp	132bp	9%	296.8M
aih-tih-sc-776623-R1_A23YTGFLT4_1_trimmed_2																																																		84.7%	56%	124bp	132bp	18%	296.8M	84.7%	56%	124bp	132bp	18%	296.8M
aih-tih-sc-790e56-R1_A23YTGFLT4_1	9999000000	338645199	325275426	0.9341	No	0.00%	339637332	0.01%	0.37%	213367850	0.93%	0.95%	0.06%	0.05%	198817735	100364131	12473045	4847425	360.23	181.00	0.72%	0.60%	0.97	0.000	137.1bp	126.0bp	0.02%	0.66%	0.35%	0.00%	0.04%	2.04%	0.53%	0.00%	0.01				37.4%	87.2%	94.9%	161.2M	22.0%	97.3%	40937.1Mb	339.6M	55.1%	98.9%	62.8%
aih-tih-sc-790e56-R1_A23YTGFLT4_1_1																																				137.1bp	96.9%	164.5M												75.3%	53%	151bp	151bp	18%	171.7M
aih-tih-sc-790e56-R1_A23YTGFLT4_1_2																																																		74.8%	54%	151bp	151bp	18%	171.7M
aih-tih-sc-790e56-R1_A23YTGFLT4_1_trimmed_1																																																		75.3%	54%	124bp	132bp	9%	169.8M	75.3%	54%	124bp	132bp	9%	169.8M
aih-tih-sc-790e56-R1_A23YTGFLT4_1_trimmed_2																																																		75.5%	55%	124bp	132bp	9%	169.8M	75.5%	55%	124bp	132bp	9%	169.8M
aih-tih-sc-7f9674-R1_A23YTGFLT4_1	9999000000	391189101	578029880	0.8977	No	0.00%	593424758	0.00%	0.49%	303256292	0.94%	0.96%	0.05%	0.04%	284310897	143494298	16241079	6126362	524.29	243.00	0.76%	0.64%	0.97	0.000	133.2bp	121.0bp	0.03%	0.91%	0.35%	0.00%	0.07%	2.99%	0.56%	0.00%	0.03				49.1%	91.2%	96.5%	286.3M	29.4%	97.3%	71079.2Mb	593.4M	56.2%	98.9%	63.0%
aih-tih-sc-7f9674-R1_A23YTGFLT4_1_1																																				133.2bp	98.0%	290.7M												85.1%	54%	151bp	151bp	18%	399.8M
aih-tih-sc-7f9674-R1_A23YTGFLT4_1_2																																																		84.3%	55%	151bp	151bp	18%	399.8M
aih-tih-sc-7f9674-R1_A23YTGFLT4_1_trimmed_1																																																		83.2%	55%	123bp	132bp	9%	296.7M	83.2%	55%	123bp	132bp	9%	296.7M
aih-tih-sc-7f9674-R1_A23YTGFLT4_1_trimmed_2																																																		83.3%	56%	123bp	132bp	9%	296.7M	83.3%	56%	123bp	132bp	9%	296.7M
aih-tih-sc-7fa0fb-R1_A23YTGFLT4_1	9990000000	138328727	359160713	0.8609	No	0.00%	372955428	0.01%	0.61%	146073351	0.90%	0.92%	0.09%	0.08%	131060967	65783108	12977056	5884202	191.60	88.00	0.66%	0.47%	0.97	0.000	108.5bp	101.0bp	0.05%	0.84%	0.31%	0.00%	0.12%	2.67%	0.49%	0.00%	0.03				61.2%	87.7%	95.4%	177.8M	29.5%	97.4%	39228.7Mb	373.0M	54.8%	98.7%	83.0%
aih-tih-sc-7fa0fb-R1_A23YTGFLT4_1_1																																				108.5bp	97.1%	181.0M												80.8%	53%	151bp	151bp	18%	188.9M
aih-tih-sc-7fa0fb-R1_A23YTGFLT4_1_2																																																		79.9%	54%	151bp	151bp	27%	188.9M
aih-tih-sc-7fa0fb-R1_A23YTGFLT4_1_trimmed_1																																																		80.9%	54%	108bp	107bp	9%	186.5M	80.9%	54%	108bp	107bp	9%	186.5M
aih-tih-sc-7fa0fb-R1_A23YTGFLT4_1_trimmed_2																																																		80.7%	54%	108bp	107bp	9%	186.5M	80.7%	54%	108bp	107bp	9%	186.5M
aih-tih-sc-816ec7-R1_A23YTGFLT4_1	9999000000	373641925	573534903	0.9163	No	0.00%	593370558	0.01%	0.48%	310841479	0.93%	0.96%	0.05%	0.04%	290609841	146752529	16969399	6304722	542.57	256.00	0.73%	0.63%	0.97	0.000	139.1bp	127.0bp	0.04%	0.99%	0.52%	0.00%	0.08%	2.97%	0.78%	0.00%	5.89				47.9%	89.1%	95.8%	284.3M	30.6%	97.2%	72467.9Mb	593.4M	55.9%	98.9%	61.0%
aih-tih-sc-816ec7-R1_A23YTGFLT4_1_1																																				139.1bp	97.8%	290.2M												84.6%	54%	151bp	151bp	18%	379.7M
aih-tih-sc-816ec7-R1_A23YTGFLT4_1_2																																																		83.9%	55%	151bp	151bp	18%	379.7M
aih-tih-sc-816ec7-R1_A23YTGFLT4_1_trimmed_1																																																		82.9%	55%	126bp	132bp	9%	296.7M	82.9%	55%	126bp	132bp	9%	296.7M
aih-tih-sc-816ec7-R1_A23YTGFLT4_1_trimmed_2																																																		83.1%	56%	126bp	132bp	9%	296.7M	83.1%	56%	126bp	132bp	9%	296.7M
aih-tih-sc-885658-R1_A23YTGFLT4_1	9999000000	196660712	522211385	0.8894	No	0.00%	541209348	0.01%	0.61%	209996180	0.90%	0.93%	0.08%	0.07%	189228689	95441738	17377387	7346341	328.59	168.00	0.73%	0.59%	0.97	0.000	135.0bp	125.0bp	0.03%	0.76%	0.26%	0.00%	0.06%	2.36%	0.42%	0.00%	0.04				61.6%	88.1%	95.6%	258.8M	40.3%	97.2%	65657.4Mb	541.2M	56.1%	98.9%	64.6%
aih-tih-sc-885658-R1_A23YTGFLT4_1_1																																				135.0bp	96.9%	262.2M												81.4%	54%	151bp	151bp	18%	273.7M
aih-tih-sc-885658-R1_A23YTGFLT4_1_2																																																		80.8%	55%	151bp	151bp	18%	273.7M
aih-tih-sc-885658-R1_A23YTGFLT4_1_trimmed_1																																																		81.6%	55%	125bp	132bp	9%	270.6M	81.6%	55%	125bp	132bp	9%	270.6M
aih-tih-sc-885658-R1_A23YTGFLT4_1_trimmed_2																																																		81.7%	56%	125bp	132bp	9%	270.6M	81.7%	56%	125bp	132bp	9%	270.6M
aih-tih-sc-89f3a3-R1_A23YTGFLT4_1	9999000000	490646147	575937332	0.9040	No	0.00%	593885744	0.00%	0.42%	344762162	0.94%	0.96%	0.06%	0.04%	322692641	163488482	19160699	6680241	642.13	317.00	0.76%	0.66%	0.97	0.000	152.2bp	139.0bp	0.03%	0.69%	0.33%	0.00%	0.07%	2.10%	0.49%	0.00%	7.82				42.2%	90.1%	96.2%	285.6M	29.1%	97.2%	75346.6Mb	593.9M	55.3%	99.0%	52.0%
aih-tih-sc-89f3a3-R1_A23YTGFLT4_1_1																																				152.2bp	97.6%	289.9M												85.6%	54%	151bp	151bp	18%	445.4M
aih-tih-sc-89f3a3-R1_A23YTGFLT4_1_2																																																		85.0%	55%	151bp	151bp	18%	445.4M
aih-tih-sc-89f3a3-R1_A23YTGFLT4_1_trimmed_1																																																		83.0%	55%	130bp	147bp	9%	296.9M	83.0%	55%	130bp	147bp	9%	296.9M
aih-tih-sc-89f3a3-R1_A23YTGFLT4_1_trimmed_2																																																		83.1%	55%	131bp	147bp	9%	296.9M	83.1%	55%	131bp	147bp	9%	296.9M
aih-tih-sc-8c7069-R1_A23YTGFLT4_1	9999000000	171771880	461004994	0.8775	Yes	0.62%	480575238	0.01%	0.62%	184227095	0.91%	0.93%	0.08%	0.07%	166840859	83904499	14545738	6389168	261.18	133.00	0.72%	0.56%	0.97	0.000	119.1bp	111.0bp	0.04%	0.61%	0.26%	0.00%	0.09%	2.01%	0.45%	0.00%	0.02				62.0%	85.4%	95.0%	228.2M	35.1%	97.3%	53972.6Mb	480.6M	56.3%	99.0%	76.4%
aih-tih-sc-8c7069-R1_A23YTGFLT4_1_1																																				119.1bp	97.3%	233.7M												81.6%	55%	151bp	151bp	18%	242.8M
aih-tih-sc-8c7069-R1_A23YTGFLT4_1_2																																																		80.9%	55%	151bp	151bp	18%	242.8M
aih-tih-sc-8c7069-R1_A23YTGFLT4_1_trimmed_1																																																		81.7%	56%	115bp	117bp	9%	240.3M	81.7%	56%	115bp	117bp	9%	240.3M
aih-tih-sc-8c7069-R1_A23YTGFLT4_1_trimmed_2																																																		81.7%	56%	115bp	117bp	9%	240.3M	81.7%	56%	115bp	117bp	9%	240.3M
aih-tih-sc-8e3550-R1_A23YTGFLT4_1	9999000000	378214594	299459905	0.9399	No	0.00%	309940838	0.01%	0.34%	203116073	0.94%	0.96%	0.05%	0.04%	190944775	96537617	10485343	3695271	374.85	168.00	0.71%	0.59%	0.97	0.000	148.5bp	135.0bp	0.02%	1.22%	0.50%	0.00%	0.05%	3.72%	0.73%	0.00%	0.01				34.7%	89.2%	95.8%	148.5M	22.3%	97.3%	38742.7Mb	309.9M	55.2%	98.7%	54.2%
aih-tih-sc-8e3550-R1_A23YTGFLT4_1_1																																				148.5bp	97.6%	151.3M												78.5%	54%	151bp	151bp	18%	157.0M
aih-tih-sc-8e3550-R1_A23YTGFLT4_1_2																																																		77.9%	55%	151bp	151bp	18%	157.0M
aih-tih-sc-8e3550-R1_A23YTGFLT4_1_trimmed_1																																																		78.6%	54%	128bp	142bp	9%	155.0M	78.6%	54%	128bp	142bp	9%	155.0M
aih-tih-sc-8e3550-R1_A23YTGFLT4_1_trimmed_2																																																		78.8%	55%	129bp	142bp	9%	155.0M	78.8%	55%	129bp	142bp	9%	155.0M
aih-tih-sc-92d360-R1_A23YTGFLT4_1	9999000000	183563198	462870773	0.9204	No	0.00%	484183660	0.00%	0.60%	191680710	0.91%	0.93%	0.07%	0.07%	175112078	87977161	14163134	6190789	271.39	126.00	0.69%	0.54%	0.98	0.000	116.6bp	109.0bp	0.05%	1.08%	0.41%	0.00%	0.10%	3.42%	0.70%	0.00%	0.01				60.7%	84.4%	94.6%	229.1M	32.8%	97.4%	53635.4Mb	484.2M	54.6%	98.8%	78.0%
aih-tih-sc-92d360-R1_A23YTGFLT4_1_1																																				116.6bp	97.5%	235.9M												81.8%	53%	151bp	151bp	18%	245.1M
aih-tih-sc-92d360-R1_A23YTGFLT4_1_2																																																		80.8%	53%	151bp	151bp	27%	245.1M
aih-tih-sc-92d360-R1_A23YTGFLT4_1_trimmed_1																																																		81.8%	54%	114bp	112bp	9%	242.1M	81.8%	54%	114bp	112bp	9%	242.1M
aih-tih-sc-92d360-R1_A23YTGFLT4_1_trimmed_2																																																		81.7%	54%	114bp	112bp	9%	242.1M	81.7%	54%	114bp	112bp	9%	242.1M
aih-tih-sc-966ec5-R1_A23YTGFLT4_1	9999000000	277092316	568639468	0.8694	Yes	0.00%	591417416	0.01%	0.55%	265204519	0.92%	0.95%	0.06%	0.05%	245268729	123337342	16479504	6834072	406.62	187.00	0.72%	0.60%	0.97	0.000	122.1bp	114.0bp	0.02%	0.95%	0.46%	0.00%	0.04%	2.89%	0.69%	0.00%	0.02				55.5%	87.1%	95.2%	281.5M	31.7%	97.2%	67483.7Mb	591.4M	56.3%	98.6%	73.5%
aih-tih-sc-966ec5-R1_A23YTGFLT4_1_1																																				122.1bp	97.4%	288.0M												83.7%	55%	151bp	151bp	18%	349.2M
aih-tih-sc-966ec5-R1_A23YTGFLT4_1_2																																																		82.6%	55%	151bp	151bp	27%	349.2M
aih-tih-sc-966ec5-R1_A23YTGFLT4_1_trimmed_1																																																		82.4%	56%	117bp	117bp	9%	295.7M	82.4%	56%	117bp	117bp	9%	295.7M
aih-tih-sc-966ec5-R1_A23YTGFLT4_1_trimmed_2																																																		82.5%	56%	118bp	117bp	9%	295.7M	82.5%	56%	118bp	117bp	9%	295.7M
aih-tih-sc-96d0f6-R1_A23YTGFLT4_1	9999000000	237016808	572715055	0.9281	No	0.00%	592751432	0.01%	0.58%	247022941	0.93%	0.95%	0.06%	0.05%	229330057	115472932	14697696	5923853	387.57	180.00	0.73%	0.60%	0.97	0.000	124.5bp	114.0bp	0.02%	0.88%	0.53%	0.00%	0.04%	2.73%	0.81%	0.00%	0.01				58.6%	89.0%	95.7%	283.5M	32.8%	97.4%	68243.7Mb	592.8M	55.5%	98.8%	71.0%
aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_1																																				124.5bp	97.8%	289.7M												84.9%	54%	151bp	151bp	18%	353.1M
aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_2																																																		84.2%	54%	151bp	151bp	18%	353.1M
aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_trimmed_1																																																		83.7%	55%	118bp	122bp	9%	296.4M	83.7%	55%	118bp	122bp	9%	296.4M
aih-tih-sc-96d0f6-R1_A23YTGFLT4_1_trimmed_2																																																		83.9%	55%	118bp	122bp	9%	296.4M	83.9%	55%	118bp	122bp	9%	296.4M
aih-tih-sc-9703f5-R1_A23YTGFLT4_1	9999000000	115016025	323021517	0.9453	No	0.00%	336944024	0.01%	0.68%	109445260	0.88%	0.90%	0.10%	0.10%	95888116	48118739	11234953	5173330	135.42	58.00	0.61%	0.38%	0.98	0.000	109.6bp	104.0bp	0.03%	1.07%	0.41%	0.00%	0.06%	3.29%	0.64%	0.00%	0.04				68.0%	85.7%	94.9%	159.9M	34.9%	97.4%	35741.2Mb	336.9M	55.7%	98.9%	84.8%
aih-tih-sc-9703f5-R1_A23YTGFLT4_1_1																																				109.6bp	96.7%	162.9M												79.8%	54%	151bp	151bp	18%	170.4M
aih-tih-sc-9703f5-R1_A23YTGFLT4_1_2																																																		78.8%	54%	151bp	151bp	27%	170.4M
aih-tih-sc-9703f5-R1_A23YTGFLT4_1_trimmed_1																																																		79.8%	55%	109bp	107bp	9%	168.5M	79.8%	55%	109bp	107bp	9%	168.5M
aih-tih-sc-9703f5-R1_A23YTGFLT4_1_trimmed_2																																																		79.5%	55%	109bp	107bp	9%	168.5M	79.5%	55%	109bp	107bp	9%	168.5M
aih-tih-sc-992d78-R1_A23YTGFLT4_1	9999000000	297196490	519132986	0.9354	No	0.00%	537446608	0.00%	0.51%	265871378	0.93%	0.95%	0.06%	0.05%	247727759	124807396	15811326	6309971	413.50	184.00	0.71%	0.59%	0.97	0.000	123.1bp	112.0bp	0.06%	1.18%	0.49%	0.00%	0.12%	3.27%	0.70%	0.00%	3.62				50.8%	89.2%	95.6%	256.8M	28.7%	97.4%	61026.1Mb	537.4M	54.1%	98.7%	72.0%
aih-tih-sc-992d78-R1_A23YTGFLT4_1_1																																				123.1bp	97.7%	262.5M												83.0%	53%	151bp	151bp	18%	272.2M
aih-tih-sc-992d78-R1_A23YTGFLT4_1_2																																																		82.2%	54%	151bp	151bp	18%	272.2M
aih-tih-sc-992d78-R1_A23YTGFLT4_1_trimmed_1																																																		83.0%	53%	117bp	117bp	9%	268.7M	83.0%	53%	117bp	117bp	9%	268.7M
aih-tih-sc-992d78-R1_A23YTGFLT4_1_trimmed_2																																																		83.2%	54%	117bp	117bp	9%	268.7M	83.2%	54%	117bp	117bp	9%	268.7M
aih-tih-sc-9b89b4-R1_A23YTGFLT4_1	9999000000	365626981	576362211	0.9106	No	0.00%	593147514	0.00%	0.51%	293592441	0.94%	0.96%	0.05%	0.04%	274870605	138583724	15929576	6155644	497.32	231.00	0.75%	0.63%	0.97	0.000	132.3bp	121.0bp	0.03%	0.85%	0.36%	0.00%	0.07%	2.59%	0.56%	0.00%	0.01				50.7%	90.6%	96.3%	285.6M	29.6%	97.2%	70586.5Mb	593.1M	56.0%	98.9%	65.4%
aih-tih-sc-9b89b4-R1_A23YTGFLT4_1_1																																				132.3bp	97.9%	290.5M												81.8%	54%	151bp	151bp	18%	328.9M
aih-tih-sc-9b89b4-R1_A23YTGFLT4_1_2																																																		80.9%	55%	151bp	151bp	18%	328.9M
aih-tih-sc-9b89b4-R1_A23YTGFLT4_1_trimmed_1																																																		81.2%	55%	122bp	127bp	9%	296.6M	81.2%	55%	122bp	127bp	9%	296.6M
aih-tih-sc-9b89b4-R1_A23YTGFLT4_1_trimmed_2																																																		81.1%	56%	122bp	127bp	9%	296.6M	81.1%	56%	122bp	127bp	9%	296.6M
aih-tih-sc-9e2647-R1_A23YTGFLT4_1	9999000000	455982070	575064262	0.9059	No	0.00%	593495710	0.00%	0.44%	329891718	0.94%	0.96%	0.05%	0.04%	309507089	156754387	17919412	6193259	616.34	266.00	0.73%	0.63%	0.97	0.000	149.6bp	135.0bp	0.09%	1.90%	0.48%	0.00%	0.18%	5.40%	0.74%	0.00%	6.24				44.6%	90.1%	96.0%	284.9M	30.2%	97.4%	75065.3Mb	593.5M	54.2%	98.9%	52.6%
aih-tih-sc-9e2647-R1_A23YTGFLT4_1_1																																				149.6bp	97.6%	289.6M												84.4%	53%	151bp	151bp	18%	330.3M
aih-tih-sc-9e2647-R1_A23YTGFLT4_1_2																																																		83.9%	54%	151bp	151bp	18%	330.3M
aih-tih-sc-9e2647-R1_A23YTGFLT4_1_trimmed_1																																																		83.9%	53%	130bp	147bp	9%	296.7M	83.9%	53%	130bp	147bp	9%	296.7M
aih-tih-sc-9e2647-R1_A23YTGFLT4_1_trimmed_2																																																		84.2%	54%	130bp	147bp	9%	296.7M	84.2%	54%	130bp	147bp	9%	296.7M
aih-tih-sc-a51633-R1_A23YTGFLT4_1	9999000000	416796243	566709574	0.8630	No	0.00%	592543670	0.01%	0.46%	322475334	0.93%	0.96%	0.05%	0.04%	300865447	152424714	17365653	5782333	612.10	243.00	0.74%	0.63%	0.97	0.000	156.1bp	141.0bp	0.06%	0.79%	0.36%	0.00%	0.14%	2.44%	0.59%	0.00%	6.19				45.9%	86.0%	94.8%	280.9M	31.6%	97.1%	75996.1Mb	592.5M	58.3%	98.8%	48.7%
aih-tih-sc-a51633-R1_A23YTGFLT4_1_1																																				156.1bp	97.3%	288.4M												86.9%	57%	151bp	151bp	18%	514.9M
aih-tih-sc-a51633-R1_A23YTGFLT4_1_2																																																		86.1%	58%	151bp	151bp	18%	514.9M
aih-tih-sc-a51633-R1_A23YTGFLT4_1_trimmed_1																																																		83.5%	58%	132bp	151bp	9%	296.3M	83.5%	58%	132bp	151bp	9%	296.3M
aih-tih-sc-a51633-R1_A23YTGFLT4_1_trimmed_2																																																		83.7%	58%	132bp	151bp	9%	296.3M	83.7%	58%	132bp	151bp	9%	296.3M
aih-tih-sc-a558c6-R1_A23YTGFLT4_1	9999000000	93511783	281635658	0.9123	No	0.00%	295175774	0.01%	0.69%	91981936	0.87%	0.90%	0.11%	0.10%	79940567	40116090	9864757	4539572	113.82	48.00	0.58%	0.34%	0.97	0.000	112.5bp	106.0bp	0.01%	1.13%	0.31%	0.00%	0.03%	3.52%	0.52%	0.00%	0.04				69.3%	84.4%	94.5%	139.4M	37.4%	97.4%	31729.8Mb	295.2M	57.1%	98.6%	82.7%
aih-tih-sc-a558c6-R1_A23YTGFLT4_1_1																																				112.5bp	96.6%	142.6M												80.1%	55%	151bp	151bp	18%	149.6M
aih-tih-sc-a558c6-R1_A23YTGFLT4_1_2																																																		79.1%	55%	151bp	151bp	18%	149.6M
aih-tih-sc-a558c6-R1_A23YTGFLT4_1_trimmed_1																																																		80.1%	56%	110bp	107bp	9%	147.6M	80.1%	56%	110bp	107bp	9%	147.6M
aih-tih-sc-a558c6-R1_A23YTGFLT4_1_trimmed_2																																																		80.0%	57%	110bp	107bp	9%	147.6M	80.0%	57%	110bp	107bp	9%	147.6M
aih-tih-sc-a8a787-R1_A23YTGFLT4_1	9999000000	505297949	574078006	0.8977	No	0.00%	593329750	0.00%	0.43%	335595899	0.95%	0.97%	0.05%	0.03%	317294770	160736433	15658305	4949422	651.09	298.00	0.75%	0.65%	0.97	0.000	155.6bp	140.0bp	0.03%	1.08%	0.62%	0.00%	0.05%	3.15%	0.87%	0.00%	0.01				43.6%	90.1%	96.0%	284.7M	29.4%	97.3%	75817.2Mb	593.3M	55.5%	98.9%	49.7%
aih-tih-sc-a8a787-R1_A23YTGFLT4_1_1																																				155.6bp	98.0%	290.7M												86.5%	54%	151bp	151bp	18%	544.3M
aih-tih-sc-a8a787-R1_A23YTGFLT4_1_2																																																		85.7%	55%	151bp	151bp	18%	544.3M
aih-tih-sc-a8a787-R1_A23YTGFLT4_1_trimmed_1																																																		82.8%	55%	131bp	151bp	9%	296.7M	82.8%	55%	131bp	151bp	9%	296.7M
aih-tih-sc-a8a787-R1_A23YTGFLT4_1_trimmed_2																																																		82.9%	55%	131bp	151bp	9%	296.7M	82.9%	55%	131bp	151bp	9%	296.7M
aih-tih-sc-a97ce1-R1_A23YTGFLT4_1	9999000000	105147150	413650898	0.9280	No	0.00%	432878552	0.01%	0.75%	106399511	0.86%	0.90%	0.11%	0.10%	91141385	45840014	11853631	5317434	138.86	53.00	0.60%	0.36%	0.97	0.000	121.6bp	114.0bp	0.04%	0.78%	0.29%	0.00%	0.09%	2.46%	0.48%	0.00%	0.02				76.0%	85.0%	94.6%	204.7M	45.6%	97.2%	49295.1Mb	432.9M	58.0%	98.7%	75.4%
aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_1																																				121.6bp	96.9%	209.8M												86.2%	55%	151bp	151bp	18%	219.2M
aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_2																																																		85.2%	56%	151bp	151bp	27%	219.2M
aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_trimmed_1																																																		86.2%	57%	117bp	117bp	9%	216.4M	86.2%	57%	117bp	117bp	9%	216.4M
aih-tih-sc-a97ce1-R1_A23YTGFLT4_1_trimmed_2																																																		86.2%	58%	117bp	117bp	9%	216.4M	86.2%	58%	117bp	117bp	9%	216.4M
aih-tih-sc-a9fe91-R1_A23YTGFLT4_1	9999000000	472515807	560703473	0.8943	Yes	0.00%	593245102	0.01%	0.44%	331851196	0.94%	0.96%	0.05%	0.04%	311245710	157593061	17117893	5841538	616.72	271.00	0.74%	0.64%	0.97	0.000	148.7bp	135.0bp	0.04%	0.71%	0.55%	0.00%	0.09%	2.27%	0.84%	0.00%	4.90				44.3%	84.4%	93.8%	278.1M	28.3%	97.3%	74635.4Mb	593.2M	56.5%	98.9%	53.6%
aih-tih-sc-a9fe91-R1_A23YTGFLT4_1_1																																				148.7bp	97.8%	290.1M												85.2%	55%	151bp	151bp	18%	525.4M
aih-tih-sc-a9fe91-R1_A23YTGFLT4_1_2																																																		84.7%	56%	151bp	151bp	18%	525.4M
aih-tih-sc-a9fe91-R1_A23YTGFLT4_1_trimmed_1																																																		81.4%	56%	129bp	142bp	9%	296.6M	81.4%	56%	129bp	142bp	9%	296.6M
aih-tih-sc-a9fe91-R1_A23YTGFLT4_1_trimmed_2																																																		81.8%	56%	129bp	142bp	9%	296.6M	81.8%	56%	129bp	142bp	9%	296.6M
aih-tih-sc-b567d0-R1_A23YTGFLT4_1	9999000000	392875162	446882544	0.9526	No	0.00%	460622242	0.00%	0.41%	270798770	0.94%	0.96%	0.05%	0.04%	253903698	128106991	14695345	5589194	472.30	226.00	0.75%	0.63%	0.97	0.000	139.2bp	127.0bp	0.05%	1.15%	0.44%	0.00%	0.10%	3.41%	0.65%	0.00%	0.02				41.4%	90.4%	96.2%	221.5M	24.8%	97.4%	56076.9Mb	460.6M	54.1%	98.9%	61.4%
aih-tih-sc-b567d0-R1_A23YTGFLT4_1_1																																				139.2bp	97.6%	224.8M												79.0%	52%	151bp	151bp	18%	233.0M
aih-tih-sc-b567d0-R1_A23YTGFLT4_1_2																																																		78.4%	54%	151bp	151bp	18%	233.0M
aih-tih-sc-b567d0-R1_A23YTGFLT4_1_trimmed_1																																																		79.0%	53%	125bp	132bp	9%	230.3M	79.0%	53%	125bp	132bp	9%	230.3M
aih-tih-sc-b567d0-R1_A23YTGFLT4_1_trimmed_2																																																		79.2%	54%	125bp	132bp	9%	230.3M	79.2%	54%	125bp	132bp	9%	230.3M
aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1	9999000000	291968844	571527437	0.9432	No	0.00%	593640818	0.00%	0.54%	271782662	0.93%	0.96%	0.06%	0.04%	253639784	127929525	15261579	5869605	453.51	217.00	0.76%	0.63%	0.97	0.000	133.0bp	122.0bp	0.03%	0.72%	0.32%	0.00%	0.05%	2.14%	0.46%	0.00%	0.01				54.5%	88.1%	95.5%	283.4M	33.3%	97.2%	70788.0Mb	593.6M	55.5%	98.9%	65.8%
aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_1																																				133.0bp	98.0%	291.0M												85.9%	54%	151bp	151bp	18%	435.0M
aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_2																																																		85.0%	55%	151bp	151bp	18%	435.0M
aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_trimmed_1																																																		83.3%	55%	123bp	127bp	9%	296.8M	83.3%	55%	123bp	127bp	9%	296.8M
aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1_trimmed_2																																																		83.2%	55%	123bp	127bp	9%	296.8M	83.2%	55%	123bp	127bp	9%	296.8M
aih-tih-sc-b6fd19-R1_A23YTGFLT4_1	9999000000	398742185	575184954	0.9100	No	0.00%	593371230	0.01%	0.46%	318113142	0.94%	0.96%	0.05%	0.04%	297947162	151062563	17103345	5710734	596.87	274.00	0.76%	0.65%	0.97	0.000	153.1bp	138.0bp	0.06%	0.88%	0.37%	0.00%	0.12%	2.65%	0.58%	0.00%	5.40				46.6%	89.7%	96.0%	284.8M	31.8%	97.3%	75222.2Mb	593.4M	56.0%	98.9%	51.0%
aih-tih-sc-b6fd19-R1_A23YTGFLT4_1_1																																				153.1bp	97.5%	289.4M												86.2%	54%	151bp	151bp	18%	417.3M
aih-tih-sc-b6fd19-R1_A23YTGFLT4_1_2																																																		85.6%	55%	151bp	151bp	18%	417.3M
aih-tih-sc-b6fd19-R1_A23YTGFLT4_1_trimmed_1																																																		84.1%	55%	130bp	147bp	9%	296.7M	84.1%	55%	130bp	147bp	9%	296.7M
aih-tih-sc-b6fd19-R1_A23YTGFLT4_1_trimmed_2																																																		84.2%	56%	130bp	147bp	9%	296.7M	84.2%	56%	130bp	147bp	9%	296.7M
aih-tih-sc-b8457f-R1_A23YTGFLT4_1	9999000000	581986569	567542604	0.9423	No	0.00%	593779020	0.00%	0.40%	354198984	0.94%	0.97%	0.05%	0.03%	333504151	168920889	17940850	5965840	678.17	302.00	0.73%	0.64%	0.97	0.000	152.6bp	138.0bp	0.03%	1.49%	0.57%	0.00%	0.06%	4.33%	0.83%	0.00%	0.02				40.5%	86.7%	94.8%	281.3M	27.4%	97.3%	75523.5Mb	593.8M	54.8%	99.0%	51.1%
aih-tih-sc-b8457f-R1_A23YTGFLT4_1_1																																				152.6bp	97.7%	290.2M												85.2%	54%	151bp	151bp	18%	421.4M
aih-tih-sc-b8457f-R1_A23YTGFLT4_1_2																																																		84.4%	54%	151bp	151bp	18%	421.4M
aih-tih-sc-b8457f-R1_A23YTGFLT4_1_trimmed_1																																																		83.1%	54%	131bp	147bp	9%	296.9M	83.1%	54%	131bp	147bp	9%	296.9M
aih-tih-sc-b8457f-R1_A23YTGFLT4_1_trimmed_2																																																		83.0%	55%	131bp	147bp	9%	296.9M	83.0%	55%	131bp	147bp	9%	296.9M
aih-tih-sc-bbc28d-R1_A23YTGFLT4_1	9990000000	183840314	396710991	0.8655	No	0.00%	414099224	0.01%	0.66%	140488028	0.89%	0.91%	0.09%	0.09%	125052960	62762866	12900521	5851843	177.33	85.00	0.67%	0.47%	0.97	0.000	109.3bp	103.0bp	0.03%	1.01%	0.42%	0.00%	0.06%	2.91%	0.61%	0.00%	0.03				66.5%	86.0%	94.6%	195.9M	34.9%	97.3%	43652.0Mb	414.1M	54.8%	98.8%	85.0%
aih-tih-sc-bbc28d-R1_A23YTGFLT4_1_1																																				109.3bp	96.5%	199.8M												82.0%	53%	151bp	151bp	18%	209.6M
aih-tih-sc-bbc28d-R1_A23YTGFLT4_1_2																																																		81.0%	54%	151bp	151bp	18%	209.6M
aih-tih-sc-bbc28d-R1_A23YTGFLT4_1_trimmed_1																																																		82.1%	54%	108bp	107bp	9%	207.0M	82.1%	54%	108bp	107bp	9%	207.0M
aih-tih-sc-bbc28d-R1_A23YTGFLT4_1_trimmed_2																																																		81.8%	55%	108bp	107bp	9%	207.0M	81.8%	55%	108bp	107bp	9%	207.0M
aih-tih-sc-c35cc3-R1_A23YTGFLT4_1	9999000000	175272612	483588295	0.8774	Yes	0.18%	501372080	0.01%	0.64%	182566321	0.91%	0.93%	0.08%	0.07%	165888720	83440542	13822661	5973471	266.80	126.00	0.70%	0.54%	0.98	0.000	121.8bp	114.0bp	0.09%	1.04%	0.47%	0.00%	0.18%	3.12%	0.70%	0.00%	0.13				64.0%	87.6%	95.4%	239.2M	37.8%	97.4%	57556.1Mb	501.4M	55.0%	99.0%	74.6%
aih-tih-sc-c35cc3-R1_A23YTGFLT4_1_1																																				121.8bp	97.4%	244.1M												82.9%	53%	151bp	151bp	18%	253.3M
aih-tih-sc-c35cc3-R1_A23YTGFLT4_1_2																																																		82.1%	54%	151bp	151bp	18%	253.3M
aih-tih-sc-c35cc3-R1_A23YTGFLT4_1_trimmed_1																																																		82.9%	54%	118bp	117bp	9%	250.7M	82.9%	54%	118bp	117bp	9%	250.7M
aih-tih-sc-c35cc3-R1_A23YTGFLT4_1_trimmed_2																																																		82.9%	55%	118bp	117bp	9%	250.7M	82.9%	55%	118bp	117bp	9%	250.7M
aih-tih-sc-c400f1-R1_A23YTGFLT4_1	9999000000	295632332	568179184	0.9373	No	0.00%	592934392	0.01%	0.52%	285175764	0.93%	0.95%	0.06%	0.05%	264857203	133638726	16784535	6536745	473.46	223.00	0.73%	0.62%	0.97	0.000	133.2bp	122.0bp	0.04%	1.14%	0.43%	0.00%	0.08%	3.45%	0.68%	0.00%	4.64				52.2%	86.9%	95.0%	281.7M	32.0%	97.3%	71059.3Mb	592.9M	56.6%	98.8%	64.4%
aih-tih-sc-c400f1-R1_A23YTGFLT4_1_1																																				133.2bp	97.6%	289.3M												83.6%	55%	151bp	151bp	18%	375.3M
aih-tih-sc-c400f1-R1_A23YTGFLT4_1_2																																																		83.0%	55%	151bp	151bp	18%	375.3M
aih-tih-sc-c400f1-R1_A23YTGFLT4_1_trimmed_1																																																		81.9%	56%	123bp	127bp	9%	296.5M	81.9%	56%	123bp	127bp	9%	296.5M
aih-tih-sc-c400f1-R1_A23YTGFLT4_1_trimmed_2																																																		82.1%	56%	123bp	127bp	9%	296.5M	82.1%	56%	123bp	127bp	9%	296.5M
aih-tih-sc-c493d4-R1_A23YTGFLT4_1	9999000000	154776510	470551883	0.9415	No	0.00%	483382014	0.01%	0.67%	159679253	0.91%	0.93%	0.08%	0.07%	144753315	72766610	12157779	5331127	228.21	100.00	0.67%	0.50%	0.97	0.000	115.2bp	108.0bp	0.02%	0.82%	0.41%	0.00%	0.05%	2.90%	0.70%	0.00%	0.02				67.4%	90.4%	96.4%	232.9M	36.7%	97.3%	53789.8Mb	483.4M	55.9%	99.0%	78.4%
aih-tih-sc-c493d4-R1_A23YTGFLT4_1_1																																				115.2bp	97.8%	236.4M												83.8%	54%	151bp	151bp	18%	244.1M
aih-tih-sc-c493d4-R1_A23YTGFLT4_1_2																																																		82.9%	55%	151bp	151bp	18%	244.1M
aih-tih-sc-c493d4-R1_A23YTGFLT4_1_trimmed_1																																																		83.9%	55%	114bp	112bp	18%	241.7M	83.9%	55%	114bp	112bp	18%	241.7M
aih-tih-sc-c493d4-R1_A23YTGFLT4_1_trimmed_2																																																		83.7%	56%	114bp	112bp	18%	241.7M	83.7%	56%	114bp	112bp	18%	241.7M
aih-tih-sc-cece97-R1_A23YTGFLT4_1	9999000000	199813746	547910038	0.9304	No	0.00%	593000840	0.01%	0.62%	228145733	0.91%	0.94%	0.07%	0.06%	208364870	104925699	15710476	6543343	349.25	171.00	0.74%	0.60%	0.97	0.000	127.7bp	118.0bp	0.03%	0.65%	0.35%	0.00%	0.07%	2.12%	0.56%	0.00%	5.92				62.0%	78.8%	91.5%	271.3M	36.7%	97.3%	69453.9Mb	593.0M	57.0%	98.8%	70.1%
aih-tih-sc-cece97-R1_A23YTGFLT4_1_1																																				127.7bp	97.6%	289.3M												84.1%	55%	151bp	151bp	18%	349.0M
aih-tih-sc-cece97-R1_A23YTGFLT4_1_2																																																		83.1%	56%	151bp	151bp	18%	349.0M
aih-tih-sc-cece97-R1_A23YTGFLT4_1_trimmed_1																																																		82.9%	56%	120bp	122bp	9%	296.5M	82.9%	56%	120bp	122bp	9%	296.5M
aih-tih-sc-cece97-R1_A23YTGFLT4_1_trimmed_2																																																		82.7%	57%	120bp	122bp	9%	296.5M	82.7%	57%	120bp	122bp	9%	296.5M
aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1	9999000000	284919873	575724597	0.9387	Yes	0.00%	594440514	0.01%	0.55%	267121207	0.93%	0.95%	0.06%	0.05%	248217721	125108392	15412764	6184855	434.46	218.00	0.76%	0.64%	0.97	0.000	128.5bp	119.0bp	0.03%	0.77%	0.38%	0.00%	0.07%	2.56%	0.60%	0.00%	0.01				55.4%	89.7%	96.0%	285.2M	31.9%	97.3%	70224.0Mb	594.4M	55.5%	99.1%	68.4%
aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_1																																				128.5bp	97.8%	290.7M												83.1%	53%	151bp	151bp	18%	364.2M
aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_2																																																		82.6%	54%	151bp	151bp	18%	364.2M
aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_trimmed_1																																																		81.6%	55%	121bp	127bp	9%	297.2M	81.6%	55%	121bp	127bp	9%	297.2M
aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1_trimmed_2																																																		81.7%	55%	121bp	127bp	9%	297.2M	81.7%	55%	121bp	127bp	9%	297.2M
aih-tih-sc-d5fda9-R1_A23YTGFLT4_1	9999000000	19116407	54328423	0.8091	No	0.00%	57569804	0.01%	0.77%	13175222	0.74%	0.79%	0.21%	0.21%	9766659	4891196	2747483	1328764	12.02	5.00	0.10%	0.01%	0.97	0.000	105.2bp	100.0bp	0.01%	0.48%	0.20%	0.00%	0.02%	1.50%	0.31%	0.00%	1.11				78.0%	80.1%	93.1%	26.8M	36.6%	97.3%	5840.5Mb	57.6M	57.4%	98.2%	88.3%
aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_1																																				105.2bp	94.4%	27.2M												78.2%	55%	151bp	151bp	18%	29.3M
aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_2																																																		77.3%	56%	151bp	151bp	36%	29.3M
aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_trimmed_1																																																		78.1%	57%	104bp	102bp	9%	28.8M	78.1%	57%	104bp	102bp	9%	28.8M
aih-tih-sc-d5fda9-R1_A23YTGFLT4_1_trimmed_2																																																		77.9%	57%	104bp	102bp	9%	28.8M	77.9%	57%	104bp	102bp	9%	28.8M
aih-tih-sc-d666f1-R1_A23YTGFLT4_1	9999000000	132623466	499363606	0.8747	No	0.00%	528641666	0.01%	0.70%	160714814	0.89%	0.92%	0.09%	0.08%	142478318	71602063	13733890	6057361	208.91	91.00	0.66%	0.48%	0.97	0.000	111.6bp	104.0bp	0.03%	0.86%	0.52%	0.00%	0.06%	2.50%	0.79%	0.00%	0.02				70.2%	81.5%	93.6%	247.3M	34.3%	97.2%	56228.2Mb	528.6M	58.5%	98.7%	81.5%
aih-tih-sc-d666f1-R1_A23YTGFLT4_1_1																																				111.6bp	96.6%	255.4M												82.6%	55%	151bp	151bp	18%	267.9M
aih-tih-sc-d666f1-R1_A23YTGFLT4_1_2																																																		81.6%	57%	151bp	151bp	27%	267.9M
aih-tih-sc-d666f1-R1_A23YTGFLT4_1_trimmed_1																																																		82.7%	58%	109bp	107bp	9%	264.3M	82.7%	58%	109bp	107bp	9%	264.3M
aih-tih-sc-d666f1-R1_A23YTGFLT4_1_trimmed_2																																																		82.6%	58%	110bp	107bp	9%	264.3M	82.6%	58%	110bp	107bp	9%	264.3M
aih-tih-sc-d963a2-R1_A23YTGFLT4_1	9999000000	382932915	564752852	0.9470	No	0.00%	592704454	0.01%	0.47%	317004147	0.93%	0.96%	0.06%	0.04%	295401366	149590541	18171321	6361519	580.59	252.00	0.73%	0.63%	0.97	0.000	151.0bp	138.0bp	0.02%	0.93%	0.41%	0.00%	0.05%	2.81%	0.62%	0.00%	4.13				46.8%	85.6%	94.5%	280.1M	31.7%	97.2%	75128.7Mb	592.7M	57.3%	98.8%	52.4%
aih-tih-sc-d963a2-R1_A23YTGFLT4_1_1																																				151.0bp	97.3%	288.4M												85.2%	56%	151bp	151bp	18%	409.2M
aih-tih-sc-d963a2-R1_A23YTGFLT4_1_2																																																		84.6%	57%	151bp	151bp	18%	409.2M
aih-tih-sc-d963a2-R1_A23YTGFLT4_1_trimmed_1																																																		83.0%	57%	130bp	147bp	9%	296.4M	83.0%	57%	130bp	147bp	9%	296.4M
aih-tih-sc-d963a2-R1_A23YTGFLT4_1_trimmed_2																																																		83.5%	57%	130bp	147bp	9%	296.4M	83.5%	57%	130bp	147bp	9%	296.4M
aih-tih-sc-da06ce-R1_A23YTGFLT4_1	9999000000	152843059	571775770	0.8876	No	0.00%	592419344	0.01%	0.71%	171497743	0.90%	0.94%	0.08%	0.06%	153620682	77815958	13195977	4995491	281.63	101.00	0.68%	0.50%	0.97	0.000	141.4bp	128.0bp	0.01%	0.37%	0.24%	0.00%	0.03%	1.25%	0.43%	0.00%	0.00				71.6%	87.1%	95.6%	283.1M	46.6%	97.0%	72343.3Mb	592.4M	60.4%	98.7%	58.0%
aih-tih-sc-da06ce-R1_A23YTGFLT4_1_1																																				141.4bp	97.3%	288.1M												88.8%	58%	151bp	151bp	18%	384.2M
aih-tih-sc-da06ce-R1_A23YTGFLT4_1_2																																																		87.4%	59%	151bp	151bp	18%	384.2M
aih-tih-sc-da06ce-R1_A23YTGFLT4_1_trimmed_1																																																		87.3%	60%	126bp	137bp	18%	296.2M	87.3%	60%	126bp	137bp	18%	296.2M
aih-tih-sc-da06ce-R1_A23YTGFLT4_1_trimmed_2																																																		86.8%	60%	126bp	137bp	9%	296.2M	86.8%	60%	126bp	137bp	9%	296.2M
aih-tih-sc-e08fa3-R1_A23YTGFLT4_1	9999000000	431780935	557717276	0.9305	No	0.00%	592218322	0.01%	0.47%	316600962	0.94%	0.96%	0.05%	0.04%	297269869	150158248	15624938	5484428	575.06	275.00	0.75%	0.65%	0.97	0.000	141.1bp	127.0bp	0.06%	1.34%	0.48%	0.00%	0.13%	4.26%	0.79%	0.00%	5.66				46.8%	84.3%	93.4%	276.5M	28.9%	97.3%	72328.6Mb	592.2M	55.6%	98.7%	57.6%
aih-tih-sc-e08fa3-R1_A23YTGFLT4_1_1																																				141.1bp	98.1%	290.5M												85.6%	54%	151bp	151bp	18%	409.9M
aih-tih-sc-e08fa3-R1_A23YTGFLT4_1_2																																																		84.8%	55%	151bp	151bp	18%	409.9M
aih-tih-sc-e08fa3-R1_A23YTGFLT4_1_trimmed_1																																																		83.6%	55%	126bp	137bp	9%	296.1M	83.6%	55%	126bp	137bp	9%	296.1M
aih-tih-sc-e08fa3-R1_A23YTGFLT4_1_trimmed_2																																																		83.8%	55%	126bp	137bp	9%	296.1M	83.8%	55%	126bp	137bp	9%	296.1M
aih-tih-sc-e1cae1-R1_A23YTGFLT4_1	9999000000	104291763	335481870	0.9373	No	0.00%	350556998	0.01%	0.69%	107103846	0.86%	0.89%	0.12%	0.11%	91749461	46159761	12649563	5771696	142.35	56.00	0.59%	0.39%	0.97	0.000	121.0bp	113.0bp	0.02%	1.41%	0.39%	0.00%	0.04%	4.26%	0.59%	0.00%	0.04				70.0%	86.0%	94.8%	166.3M	39.8%	97.3%	39508.1Mb	350.6M	55.4%	98.7%	75.9%
aih-tih-sc-e1cae1-R1_A23YTGFLT4_1_1																																				121.0bp	96.3%	168.8M												82.3%	53%	151bp	151bp	18%	177.5M
aih-tih-sc-e1cae1-R1_A23YTGFLT4_1_2																																																		81.4%	54%	151bp	151bp	27%	177.5M
aih-tih-sc-e1cae1-R1_A23YTGFLT4_1_trimmed_1																																																		82.4%	55%	116bp	117bp	9%	175.3M	82.4%	55%	116bp	117bp	9%	175.3M
aih-tih-sc-e1cae1-R1_A23YTGFLT4_1_trimmed_2																																																		82.2%	55%	116bp	117bp	9%	175.3M	82.2%	55%	116bp	117bp	9%	175.3M
aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1	9999000000	352118064	544277140	0.9356	No	0.00%	562448168	0.00%	0.48%	291629713	0.93%	0.95%	0.06%	0.05%	271783948	136899330	17062664	6887006	468.90	231.00	0.75%	0.63%	0.97	0.000	127.1bp	117.0bp	0.05%	1.15%	0.50%	0.00%	0.10%	3.35%	0.73%	0.00%	0.03				48.4%	89.6%	95.8%	269.5M	27.6%	97.4%	65732.7Mb	562.4M	53.8%	98.9%	69.8%
aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1_1																																				127.1bp	97.6%	274.5M												80.7%	53%	151bp	151bp	18%	284.4M
aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1_2																																																		80.3%	53%	151bp	151bp	18%	284.4M
aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1_trimmed_1																																																		80.7%	53%	120bp	122bp	9%	281.2M	80.7%	53%	120bp	122bp	9%	281.2M
aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1_trimmed_2																																																		81.1%	53%	120bp	122bp	9%	281.2M	81.1%	53%	120bp	122bp	9%	281.2M
aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1	9999000000	169212372	464760605	0.8884	No	0.00%	482486104	0.01%	0.61%	186895090	0.90%	0.93%	0.08%	0.07%	168895388	84937527	14940345	6506493	264.87	132.00	0.71%	0.56%	0.97	0.000	117.9bp	109.0bp	0.02%	0.89%	0.36%	0.00%	0.05%	2.82%	0.57%	0.00%	0.09				61.7%	87.5%	95.4%	230.1M	33.6%	97.3%	53770.4Mb	482.5M	56.6%	98.7%	76.1%
aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_1																																				117.9bp	97.3%	234.8M												80.9%	55%	151bp	151bp	18%	244.4M
aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_2																																																		80.0%	55%	151bp	151bp	18%	244.4M
aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_trimmed_1																																																		81.0%	56%	114bp	112bp	9%	241.2M	81.0%	56%	114bp	112bp	9%	241.2M
aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1_trimmed_2																																																		80.9%	56%	115bp	117bp	9%	241.2M	80.9%	56%	115bp	117bp	9%	241.2M
aih-tih-sc-eecf9f-R1_A23YTGFLT4_1	9990000000	180219323	575460288	0.9298	No	0.00%	592438956	0.01%	0.68%	192168637	0.92%	0.96%	0.06%	0.04%	177384634	89474692	10915381	4097572	320.40	68.00	0.62%	0.44%	0.97	0.000	134.5bp	124.0bp	0.00%	0.34%	0.26%	0.00%	0.01%	1.28%	0.52%	0.00%	0.01				68.0%	89.7%	96.1%	284.7M	44.0%	96.9%	71656.1Mb	592.4M	63.6%	98.7%	62.1%
aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_1																																				134.5bp	98.2%	291.0M												91.3%	61%	151bp	151bp	27%	577.9M
aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_2																																																		90.5%	61%	151bp	151bp	18%	577.9M
aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_trimmed_1																																																		88.0%	63%	125bp	132bp	18%	296.2M	88.0%	63%	125bp	132bp	18%	296.2M
aih-tih-sc-eecf9f-R1_A23YTGFLT4_1_trimmed_2																																																		88.3%	63%	125bp	132bp	9%	296.2M	88.3%	63%	125bp	132bp	9%	296.2M
aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1	9999000000	235065008	572269137	0.9141	No	0.00%	592764628	0.01%	0.58%	246285895	0.92%	0.95%	0.06%	0.05%	227498910	114692555	15259253	6095010	401.59	198.00	0.73%	0.61%	0.97	0.000	133.5bp	124.0bp	0.05%	0.89%	0.55%	0.00%	0.10%	2.72%	0.79%	0.00%	0.01				58.8%	88.7%	95.7%	283.5M	37.9%	97.3%	71989.3Mb	592.8M	56.1%	98.8%	64.2%
aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_1																																				133.5bp	97.6%	289.4M												84.6%	54%	151bp	151bp	18%	363.2M
aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_2																																																		83.7%	55%	151bp	151bp	18%	363.2M
aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_trimmed_1																																																		83.1%	55%	125bp	132bp	9%	296.4M	83.1%	55%	125bp	132bp	9%	296.4M
aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1_trimmed_2																																																		83.1%	56%	125bp	132bp	9%	296.4M	83.1%	56%	125bp	132bp	9%	296.4M
aih-tih-sc-f26674-R1_A23YTGFLT4_1	9999000000	460177290	577613399	0.9703	No	0.00%	593168900	0.01%	0.45%	325037715	0.94%	0.96%	0.05%	0.04%	306305145	154483633	15626476	5753997	567.60	287.00	0.76%	0.66%	0.97	0.000	135.3bp	123.0bp	0.02%	0.59%	0.51%	0.00%	0.04%	1.87%	0.77%	0.00%	5.26				45.4%	91.3%	96.4%	285.9M	28.6%	97.4%	71918.1Mb	593.2M	54.6%	98.9%	62.6%
aih-tih-sc-f26674-R1_A23YTGFLT4_1_1																																				135.3bp	98.1%	290.8M												85.8%	53%	151bp	151bp	18%	425.5M
aih-tih-sc-f26674-R1_A23YTGFLT4_1_2																																																		85.1%	54%	151bp	151bp	18%	425.5M
aih-tih-sc-f26674-R1_A23YTGFLT4_1_trimmed_1																																																		83.6%	54%	124bp	132bp	9%	296.6M	83.6%	54%	124bp	132bp	9%	296.6M
aih-tih-sc-f26674-R1_A23YTGFLT4_1_trimmed_2																																																		83.8%	54%	125bp	132bp	9%	296.6M	83.8%	54%	125bp	132bp	9%	296.6M
aih-tih-sc-f27606-R1_A23YTGFLT4_1	9999000000	105446913	551298981	0.9252	No	0.00%	582954032	0.01%	0.80%	114024804	0.85%	0.90%	0.11%	0.10%	96725416	48608630	12605296	5593279	148.18	54.00	0.59%	0.38%	0.97	0.000	127.3bp	119.0bp	0.01%	0.65%	0.28%	0.00%	0.03%	2.01%	0.44%	0.00%	4.76				81.1%	82.6%	93.8%	273.3M	52.4%	97.1%	68251.2Mb	583.0M	59.1%	98.6%	71.3%
aih-tih-sc-f27606-R1_A23YTGFLT4_1_1																																				127.3bp	97.1%	283.0M												87.6%	56%	151bp	151bp	18%	295.7M
aih-tih-sc-f27606-R1_A23YTGFLT4_1_2																																																		86.4%	58%	151bp	151bp	18%	295.7M
aih-tih-sc-f27606-R1_A23YTGFLT4_1_trimmed_1																																																		87.8%	58%	120bp	122bp	9%	291.5M	87.8%	58%	120bp	122bp	9%	291.5M
aih-tih-sc-f27606-R1_A23YTGFLT4_1_trimmed_2																																																		87.5%	59%	121bp	122bp	9%	291.5M	87.5%	59%	121bp	122bp	9%	291.5M
aih-tih-sc-f2fa3a-R1_A23YTGFLT4_1	9999000000	258702228	575156443	0.9264	No	0.00%	593774010	0.01%	0.58%	249964324	0.93%	0.95%	0.06%	0.05%	231627435	116480367	15052125	6319670	377.40	176.00	0.73%	0.60%	0.97	0.000	116.9bp	109.0bp	0.03%	0.91%	0.53%	0.00%	0.06%	3.03%	0.84%	0.00%	5.49				58.2%	89.8%	95.8%	284.4M	31.2%	97.4%	66493.7Mb	593.8M	54.9%	99.0%	76.6%
aih-tih-sc-f2fa3a-R1_A23YTGFLT4_1_1																																				116.9bp	97.9%	290.7M												82.2%	53%	151bp	151bp	18%	320.7M
aih-tih-sc-f2fa3a-R1_A23YTGFLT4_1_2																																																		81.4%	54%	151bp	151bp	18%	320.7M
aih-tih-sc-f2fa3a-R1_A23YTGFLT4_1_trimmed_1																																																		81.7%	54%	115bp	117bp	9%	296.9M	81.7%	54%	115bp	117bp	9%	296.9M
aih-tih-sc-f2fa3a-R1_A23YTGFLT4_1_trimmed_2																																																		81.7%	55%	115bp	117bp	9%	296.9M	81.7%	55%	115bp	117bp	9%	296.9M
aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1	9990000000	467936960	574643850	0.8897	No	0.00%	593599284	0.01%	0.44%	332738965	0.94%	0.96%	0.05%	0.04%	311521501	157324503	17844932	6636494	591.41	280.00	0.75%	0.65%	0.97	0.000	140.8bp	129.0bp	0.02%	0.79%	0.47%	0.00%	0.05%	2.52%	0.72%	0.00%	0.01				44.2%	90.0%	95.9%	284.7M	28.0%	97.4%	73078.1Mb	593.6M	54.0%	98.9%	59.6%
aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_1																																				140.8bp	97.8%	290.2M												82.6%	53%	151bp	151bp	18%	307.2M
aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_2																																																		82.0%	54%	151bp	151bp	18%	307.2M
aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_trimmed_1																																																		82.5%	53%	126bp	137bp	9%	296.8M	82.5%	53%	126bp	137bp	9%	296.8M
aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1_trimmed_2																																																		82.7%	54%	126bp	137bp	9%	296.8M	82.7%	54%	126bp	137bp	9%	296.8M
aih-tih-sc-f7ac14-R1_A23YTGFLT4_1	9999000000	116928297	555234994	0.9241	No	0.00%	581184886	0.01%	0.76%	138430228	0.88%	0.92%	0.09%	0.08%	121863243	61163042	12471341	5582208	176.30	68.00	0.63%	0.42%	0.97	0.000	111.5bp	105.0bp	0.01%	0.55%	0.38%	0.00%	0.03%	1.70%	0.58%	0.00%	5.65				76.7%	85.4%	94.6%	274.9M	41.7%	97.3%	61798.3Mb	581.2M	58.0%	98.8%	82.5%
aih-tih-sc-f7ac14-R1_A23YTGFLT4_1_1																																				111.5bp	97.4%	283.0M												85.9%	55%	151bp	151bp	27%	294.2M
aih-tih-sc-f7ac14-R1_A23YTGFLT4_1_2																																																		85.0%	56%	151bp	151bp	27%	294.2M
aih-tih-sc-f7ac14-R1_A23YTGFLT4_1_trimmed_1																																																		86.0%	57%	109bp	107bp	9%	290.6M	86.0%	57%	109bp	107bp	9%	290.6M
aih-tih-sc-f7ac14-R1_A23YTGFLT4_1_trimmed_2																																																		86.0%	58%	109bp	107bp	9%	290.6M	86.0%	58%	109bp	107bp	9%	290.6M
aih-tih-sc-f834a3-R1_A23YTGFLT4_1	9999000000	199042486	448934922	0.8881	No	0.00%	467683832	0.01%	0.57%	202727848	0.90%	0.92%	0.09%	0.08%	181754753	91702200	18253598	7802368	319.97	147.00	0.73%	0.57%	0.97	0.000	133.9bp	124.0bp	0.04%	0.29%	0.26%	0.00%	0.11%	0.98%	0.38%	0.00%	0.07				57.0%	87.3%	95.1%	222.4M	35.2%	97.2%	56313.2Mb	467.7M	56.7%	98.7%	63.9%
aih-tih-sc-f834a3-R1_A23YTGFLT4_1_1																																				133.9bp	96.5%	225.7M												79.8%	55%	151bp	151bp	18%	236.9M
aih-tih-sc-f834a3-R1_A23YTGFLT4_1_2																																																		79.2%	55%	151bp	151bp	18%	236.9M
aih-tih-sc-f834a3-R1_A23YTGFLT4_1_trimmed_1																																																		79.8%	56%	124bp	132bp	9%	233.8M	79.8%	56%	124bp	132bp	9%	233.8M
aih-tih-sc-f834a3-R1_A23YTGFLT4_1_trimmed_2																																																		80.1%	56%	124bp	132bp	9%	233.8M	80.1%	56%	124bp	132bp	9%	233.8M
aih-tih-sc-f8efb2-R1_A23YTGFLT4_1	9999000000	164290697	568086732	0.9337	No	0.00%	592891376	0.01%	0.70%	180584968	0.89%	0.93%	0.08%	0.07%	161601744	81281231	14904797	6484978	246.10	109.00	0.70%	0.52%	0.97	0.000	115.3bp	107.0bp	0.02%	0.76%	0.47%	0.00%	0.04%	2.22%	0.67%	0.00%	0.01				70.0%	86.4%	94.9%	281.2M	38.4%	97.3%	65376.8Mb	592.9M	57.4%	98.8%	78.8%
aih-tih-sc-f8efb2-R1_A23YTGFLT4_1_1																																				115.3bp	97.2%	288.2M												85.4%	55%	151bp	151bp	18%	339.7M
aih-tih-sc-f8efb2-R1_A23YTGFLT4_1_2																																																		84.4%	56%	151bp	151bp	27%	339.7M
aih-tih-sc-f8efb2-R1_A23YTGFLT4_1_trimmed_1																																																		84.5%	57%	113bp	112bp	9%	296.4M	84.5%	57%	113bp	112bp	9%	296.4M
aih-tih-sc-f8efb2-R1_A23YTGFLT4_1_trimmed_2																																																		84.3%	57%	113bp	112bp	9%	296.4M	84.3%	57%	113bp	112bp	9%	296.4M
aih-tih-sc-fa55c3-R1_A23YTGFLT4_1	9999000000	309015968	574151351	0.8814	Yes	0.00%	593580690	0.01%	0.51%	293015224	0.93%	0.95%	0.06%	0.05%	271648640	137050412	18034418	7142819	481.94	253.00	0.77%	0.65%	0.97	0.000	131.4bp	120.0bp	0.03%	0.95%	0.41%	0.00%	0.07%	2.89%	0.62%	0.00%	6.25				50.9%	88.8%	95.8%	284.3M	30.4%	97.3%	70535.7Mb	593.6M	55.4%	98.9%	66.0%
aih-tih-sc-fa55c3-R1_A23YTGFLT4_1_1																																				131.4bp	97.6%	289.6M												81.1%	54%	151bp	151bp	18%	301.4M
aih-tih-sc-fa55c3-R1_A23YTGFLT4_1_2																																																		80.4%	54%	151bp	151bp	18%	301.4M
aih-tih-sc-fa55c3-R1_A23YTGFLT4_1_trimmed_1																																																		81.1%	55%	122bp	127bp	9%	296.8M	81.1%	55%	122bp	127bp	9%	296.8M
aih-tih-sc-fa55c3-R1_A23YTGFLT4_1_trimmed_2																																																		81.2%	55%	122bp	127bp	9%	296.8M	81.2%	55%	122bp	127bp	9%	296.8M
aih-tih-sc-fba77b-R1_A23YTGFLT4_1	9999000000	27659935	98978253	0.8253	No	0.00%	105718918	0.01%	0.80%	21484555	0.77%	0.82%	0.18%	0.18%	16510980	8266743	3907790	1872802	20.26	7.00	0.19%	0.03%	0.97	0.000	99.1bp	95.0bp	0.02%	0.57%	0.22%	0.00%	0.04%	1.85%	0.37%	0.00%	0.02				80.5%	76.6%	92.5%	48.9M	36.1%	97.1%	10221.4Mb	105.7M	58.9%	98.1%	90.9%
aih-tih-sc-fba77b-R1_A23YTGFLT4_1_1																																				99.1bp	95.5%	50.5M												81.5%	56%	151bp	151bp	27%	53.9M
aih-tih-sc-fba77b-R1_A23YTGFLT4_1_2																																																		80.4%	56%	151bp	151bp	36%	53.9M
aih-tih-sc-fba77b-R1_A23YTGFLT4_1_trimmed_1																																																		81.4%	58%	99bp	97bp	9%	52.9M	81.4%	58%	99bp	97bp	9%	52.9M
aih-tih-sc-fba77b-R1_A23YTGFLT4_1_trimmed_2																																																		81.1%	59%	100bp	97bp	9%	52.9M	81.1%	59%	100bp	97bp	9%	52.9M
aih-tih-sc-fe8311-R1_A23YTGFLT4_1	9999000000	347212644	575848216	0.9221	No	0.00%	593310838	0.01%	0.53%	281399744	0.93%	0.96%	0.05%	0.04%	262398939	132514407	15407077	5747268	493.54	220.00	0.73%	0.62%	0.97	0.000	137.4bp	126.0bp	0.02%	0.76%	0.38%	0.00%	0.05%	2.55%	0.61%	0.00%	0.01				52.9%	90.5%	96.0%	284.9M	33.3%	97.2%	73073.7Mb	593.3M	56.9%	98.9%	59.7%
aih-tih-sc-fe8311-R1_A23YTGFLT4_1_1																																				137.4bp	97.2%	288.5M												85.0%	55%	151bp	151bp	18%	395.7M
aih-tih-sc-fe8311-R1_A23YTGFLT4_1_2																																																		84.1%	56%	151bp	151bp	18%	395.7M
aih-tih-sc-fe8311-R1_A23YTGFLT4_1_trimmed_1																																																		83.1%	56%	127bp	137bp	9%	296.7M	83.1%	56%	127bp	137bp	9%	296.7M
aih-tih-sc-fe8311-R1_A23YTGFLT4_1_trimmed_2																																																		83.0%	57%	127bp	137bp	9%	296.7M	83.0%	57%	127bp	137bp	9%	296.7M
aih-tih-sc-ffabe0-R1_A23YTGFLT4_1	9999000000	250234034	573353800	0.8837	No	0.00%	592574562	0.01%	0.57%	257541980	0.92%	0.94%	0.07%	0.06%	236730716	119287261	17233661	7072130	407.46	218.00	0.75%	0.63%	0.97	0.000	126.3bp	116.0bp	0.02%	0.94%	0.37%	0.00%	0.04%	3.00%	0.60%	0.00%	0.02				56.9%	89.7%	95.9%	284.0M	33.2%	97.3%	69098.5Mb	592.6M	54.6%	98.8%	68.4%
aih-tih-sc-ffabe0-R1_A23YTGFLT4_1_1																																				126.3bp	97.5%	288.9M												83.0%	53%	151bp	151bp	18%	306.9M
aih-tih-sc-ffabe0-R1_A23YTGFLT4_1_2																																																		82.1%	54%	151bp	151bp	18%	306.9M
aih-tih-sc-ffabe0-R1_A23YTGFLT4_1_trimmed_1																																																		82.9%	54%	120bp	122bp	9%	296.3M	82.9%	54%	120bp	122bp	9%	296.3M
aih-tih-sc-ffabe0-R1_A23YTGFLT4_1_trimmed_2																																																		82.8%	54%	120bp	122bp	9%	296.3M	82.8%	54%	120bp	122bp	9%	296.3M
aih-tih-sc-ffd2af-R1_A23YTGFLT4_1	9999000000	147197554	421206483	0.8998	No	0.00%	439194070	0.01%	0.67%	146974937	0.88%	0.91%	0.09%	0.09%	129652130	65112184	13903266	6307376	190.22	89.00	0.65%	0.48%	0.97	0.000	114.1bp	107.0bp	0.03%	0.80%	0.37%	0.00%	0.06%	2.45%	0.56%	0.00%	0.02				67.1%	86.7%	95.0%	208.5M	35.1%	97.4%	47583.1Mb	439.2M	55.4%	98.9%	81.8%
aih-tih-sc-ffd2af-R1_A23YTGFLT4_1_1																																				114.1bp	96.8%	212.6M												80.6%	53%	151bp	151bp	18%	222.0M
aih-tih-sc-ffd2af-R1_A23YTGFLT4_1_2																																																		79.7%	54%	151bp	151bp	27%	222.0M
aih-tih-sc-ffd2af-R1_A23YTGFLT4_1_trimmed_1																																																		80.6%	55%	111bp	112bp	9%	219.6M	80.6%	55%	111bp	112bp	9%	219.6M
aih-tih-sc-ffd2af-R1_A23YTGFLT4_1_trimmed_2																																																		80.5%	55%	111bp	112bp	9%	219.6M	80.5%	55%	111bp	112bp	9%	219.6M

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-general_stats_table

Sort	Group	Column	Description	ID	Scale
\|\|	Custom content: Preseq	Total Reads	Total reads from preseq analysis	`total_reads`	None
\|\|	Custom content: Preseq	Expected Distinct	Expected distinct molecules at maximum sequencing depth	`expected_distinct`	None
\|\|	Custom content: RSeQC	Unique Reads	Reads with mapq >= mapq_cut (uniquely mapped)	`unique_reads_rseqc`	None
\|\|	Custom content: RSeQC	Frac Antisense	Fraction of reads explained by antisense orientation (1+-,1-+,2++,2--)	`frac_antisense`	None
\|\|	Custom content: RNA contamination	RNA contaminated	Whether the sample was classified as contaminated from VAF_p90	`is_contaminated`	None
\|\|	Custom content: RNA contamination	RNA contamination %	Estimated RNA contamination from VAF_p90; 0% for samples classified as clean	`contamination_pct`	None
\|\|	Custom content: SeqTools (general stats)	Reads	SeqTools (general stats) — reads	`reads`	None
\|\|	Custom content: SeqTools (general stats)	Frac Unmapped	SeqTools (general stats) — frac unmapped	`frac_unmapped`	None
\|\|	Custom content: SeqTools (general stats)	Frac Dupes	SeqTools (general stats) — frac dupes	`frac_dupes`	None
\|\|	Custom content: SeqTools (general stats)	Unique Reads	SeqTools (general stats) — unique reads	`unique_reads`	None
\|\|	Custom content: SeqTools (general stats)	Frac Reads On Target	SeqTools (general stats) — frac reads on target	`frac_reads_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Pairs On Target	SeqTools (general stats) — frac pairs on target	`frac_pairs_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Reads Off Target	SeqTools (general stats) — frac reads off target	`frac_reads_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Frac Pairs Off Target	SeqTools (general stats) — frac pairs off target	`frac_pairs_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Reads On Target	SeqTools (general stats) — reads on target	`reads_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Pairs On Target	SeqTools (general stats) — pairs on target	`pairs_on_target`	None
\|\|	Custom content: SeqTools (general stats)	Reads Off Target	SeqTools (general stats) — reads off target	`reads_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Pairs Off Target	SeqTools (general stats) — pairs off target	`pairs_off_target`	None
\|\|	Custom content: SeqTools (general stats)	Mean Depth	SeqTools (general stats) — mean depth	`mean_depth`	None
\|\|	Custom content: SeqTools (general stats)	Q50 Depth	SeqTools (general stats) — q50 depth	`q50_depth`	None
\|\|	Custom content: SeqTools (general stats)	Frac Bases Gt30X	SeqTools (general stats) — frac bases gt30x	`frac_bases_gt30x`	None
\|\|	Custom content: SeqTools (general stats)	Frac Bases Gt100X	SeqTools (general stats) — frac bases gt100x	`frac_bases_gt100x`	None
\|\|	Custom content: SeqTools (general stats)	N Q30	SeqTools (general stats) — n q30	`n_q30`	None
\|\|	Custom content: SeqTools (general stats)	GC Bias	SeqTools (general stats) — Ratio of high GC (63-100%) to low GC (0-43%) reads	`gc_bias`	None
\|\|	Custom content: Kallisto	Fragment Length Mean	Mean fragment length from kallisto fragment length distribution	`fragment_length_mean`	None
\|\|	Custom content: Kallisto	Fragment Length Median	Median fragment length from kallisto fragment length distribution	`fragment_length_median`	None
\|\|	Custom content: Expression QC	pct Mito est. counts	Percentage of estimated counts from mitochondrial genes; high values can indicate cell damage or stress.	`pct_mito_est_counts`	None
\|\|	Custom content: Expression QC	pct Ribo est. counts	Percentage of estimated counts from ribosomal genes; useful for assessing RNA quality and technical variation.	`pct_ribo_est_counts`	None
\|\|	Custom content: Expression QC	pct Housekeeping est. counts	Percentage of estimated counts from housekeeping genes.	`pct_hk_est_counts`	None
\|\|	Custom content: Expression QC	pct Male est. counts	pct Male est. counts	`pct_male_est_counts`	None
\|\|	Custom content: Expression QC	pct Mito TPM	Percentage of TPM from mitochondrial genes; high values can indicate cell damage or stress.	`pct_mito_tpm`	None
\|\|	Custom content: Expression QC	pct Ribo TPM	Percentage of TPM from ribosomal genes; useful for assessing RNA quality and technical variation.	`pct_ribo_tpm`	None
\|\|	Custom content: Expression QC	pct Housekeeping TPM	Percentage of TPM from housekeeping genes.	`pct_hk_tpm`	None
\|\|	Custom content: Expression QC	pct Male TPM	pct Male TPM	`pct_male_tpm`	None
\|\|	Custom content: Expression QC	ChrY Signature Score	Mean log2(TPM+1) score for chrY signature genes; used for sex verification.	`score_log2tpm1_mean_chry_signature`	None
\|\|	Kallisto	Frag Length	Estimated average fragment length	`fragment_length`	None
\|\|	Kallisto	% Aligned	% processed reads that were pseudoaligned	`percent_aligned`	None
\|\|	Kallisto	M Aligned	Pseudoaligned reads (millions)	`pseudoaligned_reads`	read_count
\|\|	Picard: Mark Duplicates	Duplication	Mark Duplicates - Percent Duplication	`PERCENT_DUPLICATION`	None
\|\|	RSeQC	% Proper Pairs	% Reads mapped in proper pairs	`proper_pairs_percent`	None
\|\|	STAR	% Aligned	% Uniquely mapped reads	`uniquely_mapped_percent`	None
\|\|	STAR	M Aligned	Uniquely mapped reads (millions)	`uniquely_mapped`	read_count
\|\|	fastp	% Duplication	Duplication rate before filtering	`pct_duplication`	None
\|\|	fastp	% > Q30	Percentage of reads > Q30 after filtering	`after_filtering_q30_rate`	None
\|\|	fastp	Mb Q30 bases	Bases > Q30 after filtering (millions)	`after_filtering_q30_bases`	base_count
\|\|	fastp	M Reads After Filtering	Total reads after filtering (millions)	`filtering_result_passed_filter_reads`	read_count
\|\|	fastp	GC content	GC content after filtering	`after_filtering_gc_content`	None
\|\|	fastp	% PF	Percent reads passing filter	`pct_surviving`	None
\|\|	fastp	% Adapter	Percentage adapter-trimmed reads	`pct_adapter`	None
\|\|	FastQC (raw)	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC (raw)	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC (raw)	Average Read Length	Average Read Length (bp)	`avg_sequence_length`	None
\|\|	FastQC (raw)	Median Read Length	Median Read Length (bp)	`median_sequence_length`	None
\|\|	FastQC (raw)	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC (raw)	M Seqs	Total Sequences (millions)	`total_sequences`	read_count
\|\|	FastQC (trimmed)	% Dups	% Duplicate Reads	`percent_duplicates`	None
\|\|	FastQC (trimmed)	% GC	Average % GC Content	`percent_gc`	None
\|\|	FastQC (trimmed)	Average Read Length	Average Read Length (bp)	`avg_sequence_length`	None
\|\|	FastQC (trimmed)	Median Read Length	Median Read Length (bp)	`median_sequence_length`	None
\|\|	FastQC (trimmed)	% Failed	Percentage of modules failed in FastQC report (includes those not plotted here)	`percent_fails`	None
\|\|	FastQC (trimmed)	M Seqs	Total Sequences (millions)	`total_sequences`	read_count

Kallisto

Kallisto is a program for quantifying abundances of transcripts from RNA-Seq data.DOI: 10.1038/nbt.3519.

Created with MultiQC

Picard

Picard is a set of Java command line tools for manipulating high-throughput sequencing data.

Mark Duplicates

Number of reads, categorised by duplication state. Pair counts are doubled - see help text for details.

The table in the Picard metrics file contains some columns referring read pairs and some referring to single reads.

To make the numbers in this plot sum correctly, values referring to pairs are doubled according to the scheme below:

READS_IN_DUPLICATE_PAIRS = 2 * READ_PAIR_DUPLICATES
READS_IN_UNIQUE_PAIRS = 2 * (READ_PAIRS_EXAMINED - READ_PAIR_DUPLICATES)
READS_IN_UNIQUE_UNPAIRED = UNPAIRED_READS_EXAMINED - UNPAIRED_READ_DUPLICATES
READS_IN_DUPLICATE_PAIRS_OPTICAL = 2 * READ_PAIR_OPTICAL_DUPLICATES
READS_IN_DUPLICATE_PAIRS_NONOPTICAL = READS_IN_DUPLICATE_PAIRS - READS_IN_DUPLICATE_PAIRS_OPTICAL
READS_IN_DUPLICATE_UNPAIRED = UNPAIRED_READ_DUPLICATES
READS_UNMAPPED = UNMAPPED_READS

Created with MultiQC

Preseq

Preseq estimates the complexity of a library, showing how many additional unique reads are sequenced for increasing total read count. A shallow curve indicates complexity saturation. The dashed line shows a perfectly complex library where total reads = unique reads.DOI: 10.1038/nmeth.2375.

Complexity curve (molecule count)

Note that the x-axis is trimmed at the point where all the datasets show 80% of their maximum y-value, to avoid ridiculous scales.

Created with MultiQC

RSeQC

RSeQC package provides a number of useful modules that can comprehensively evaluate high throughput RNA-seq data.DOI: 10.1093/bioinformatics/bts356.

Read Distribution

Read Distribution calculates how mapped reads are distributed over genome features.

Created with MultiQC

Inner Distance

Inner Distance calculates the inner distance (or insert size) between two paired RNA reads. Note that this can be negative if fragments overlap.

Created with MultiQC

Read Duplication

read_duplication.py calculates how many alignment positions have a certain number of exact duplicates. Note - plot truncated at 500 occurrences and binned.

Created with MultiQC

Junction Annotation

Junction annotation compares detected splice junctions to a reference gene model. An RNA read can be spliced 2 or more times, each time is called a splicing event.

Created with MultiQC

Junction Saturation

Junction Saturation counts the number of known splicing junctions that are observed in each dataset. If sequencing depth is sufficient, all (annotated) splice junctions should be rediscovered, resulting in a curve that reaches a plateau. Missing low abundance splice junctions can affect downstream analysis.

Click a line to see the data side by side (as in the original RSeQC plot).

Created with MultiQC

Infer experiment

Infer experiment counts the percentage of reads and read pairs that match the strandedness of overlapping transcripts. It can be used to infer whether RNA-seq library preps are stranded (sense or antisense).

Created with MultiQC

Bam Stat

All numbers reported in millions.

Created with MultiQC

Showing ⁹⁶/₉₆ rows and ¹⁴/₁₄ columns.

Sample Name	Total records	QC failed	Duplicates	Non primary hit	Unmapped	Unique	Read-1	Read-2	+ve strand	-ve strand	Non-splice reads	Splice reads	Proper pairs	Different chrom
FFPE_HD789_01_RNA_13_A23YTGFLT4_1	631.5M	0.0M	0.0M	37.1M	2.5M	575.5M	287.9M	287.6M	287.8M	287.7M	273.2M	302.4M	574.0M	0.0M
FFPE_V4_0002_RNA_0002_A23YTGFLT4_1	621.9M	0.0M	0.0M	46.8M	3.3M	552.7M	276.5M	276.2M	276.4M	276.3M	285.3M	267.4M	551.1M	0.0M
NTC_0001_0001_A23YTGFLT4_1	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M	0.0M
aih-tih-sc-01c779-R1_A23YTGFLT4_1	596.5M	0.0M	0.0M	52.4M	2.8M	520.1M	260.2M	259.9M	260.1M	260.0M	295.1M	224.9M	518.2M	0.0M
aih-tih-sc-01eb49-R1_A23YTGFLT4_1	646.6M	0.0M	0.0M	51.4M	4.6M	569.2M	284.7M	284.5M	284.6M	284.5M	300.9M	268.3M	567.5M	0.0M
aih-tih-sc-0754d2-R1_A23YTGFLT4_1	72.4M	0.0M	0.0M	12.7M	0.6M	54.7M	27.4M	27.4M	27.4M	27.4M	38.7M	16.1M	54.5M	0.0M
aih-tih-sc-1274d4-R1_A23YTGFLT4_1	562.9M	0.0M	0.0M	39.2M	2.9M	504.3M	252.3M	252.0M	252.2M	252.1M	291.1M	213.2M	502.7M	0.0M
aih-tih-sc-13d441-R1_A23YTGFLT4_1	554.4M	0.0M	0.0M	36.9M	2.8M	499.7M	250.0M	249.7M	249.9M	249.8M	276.1M	223.6M	498.0M	0.0M
aih-tih-sc-150e73-R1_A23YTGFLT4_1	657.5M	0.0M	0.0M	64.1M	4.2M	563.6M	281.9M	281.6M	281.8M	281.8M	322.0M	241.5M	561.8M	0.0M
aih-tih-sc-184b05-R1_A23YTGFLT4_1	659.5M	0.0M	0.0M	64.2M	4.4M	567.0M	283.7M	283.3M	283.5M	283.5M	315.5M	251.5M	565.2M	0.0M
aih-tih-sc-242a7d-R1_A23YTGFLT4_1	555.8M	0.0M	0.0M	48.5M	3.4M	487.1M	243.8M	243.3M	243.6M	243.5M	282.4M	204.7M	485.3M	0.0M
aih-tih-sc-24ecfe-R1_A23YTGFLT4_1	78.2M	0.0M	0.0M	11.7M	0.9M	61.7M	30.9M	30.8M	30.9M	30.8M	43.3M	18.4M	61.4M	0.0M
aih-tih-sc-28eb14-R1_A23YTGFLT4_1	643.4M	0.0M	0.0M	47.8M	2.7M	574.2M	287.3M	286.9M	287.1M	287.1M	298.9M	275.3M	572.5M	0.0M
aih-tih-sc-2b9bfa-R1_A23YTGFLT4_1	666.3M	0.0M	0.0M	70.5M	3.6M	559.5M	279.9M	279.6M	279.8M	279.7M	313.5M	246.0M	557.7M	0.0M
aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1	636.3M	0.0M	0.0M	41.6M	3.5M	573.5M	286.9M	286.6M	286.8M	286.7M	293.5M	280.0M	571.7M	0.0M
aih-tih-sc-318819-R1_A23YTGFLT4_1	573.9M	0.0M	0.0M	61.9M	3.1M	484.0M	242.2M	241.8M	242.0M	242.0M	297.6M	186.4M	482.3M	0.0M
aih-tih-sc-31d31f-R1_A23YTGFLT4_1	466.0M	0.0M	0.0M	79.0M	3.4M	351.5M	175.9M	175.6M	175.8M	175.8M	230.8M	120.7M	350.5M	0.0M
aih-tih-sc-3270ab-R1_A23YTGFLT4_1	634.7M	0.0M	0.0M	40.4M	3.0M	574.3M	287.3M	287.0M	287.2M	287.1M	305.2M	269.1M	572.5M	0.0M
aih-tih-sc-3282fd-R1_A23YTGFLT4_1	534.3M	0.0M	0.0M	45.2M	2.9M	469.3M	234.8M	234.5M	234.7M	234.6M	276.4M	192.9M	467.7M	0.0M
aih-tih-sc-3377ac-R1_A23YTGFLT4_1	643.9M	0.0M	0.0M	48.1M	3.1M	574.8M	287.6M	287.1M	287.5M	287.3M	310.6M	264.2M	573.0M	0.0M
aih-tih-sc-377071-R1_A23YTGFLT4_1	638.3M	0.0M	0.0M	42.9M	3.3M	573.7M	287.0M	286.7M	286.9M	286.8M	295.8M	278.0M	571.6M	0.0M
aih-tih-sc-3abdf4-R1_A23YTGFLT4_1	534.4M	0.0M	0.0M	53.3M	3.3M	459.3M	229.8M	229.4M	229.6M	229.6M	280.2M	179.1M	457.9M	0.0M
aih-tih-sc-3b436b-R1_A23YTGFLT4_1	278.3M	0.0M	0.0M	19.7M	1.7M	248.5M	124.3M	124.2M	124.3M	124.3M	126.3M	122.2M	247.7M	0.0M
aih-tih-sc-45523c-R1_A23YTGFLT4_1	525.3M	0.0M	0.0M	41.3M	3.0M	464.7M	232.6M	232.1M	232.4M	232.3M	265.9M	198.8M	463.3M	0.0M
aih-tih-sc-49909d-R1_A23YTGFLT4_1	595.3M	0.0M	0.0M	61.6M	4.0M	509.2M	254.8M	254.4M	254.6M	254.6M	302.5M	206.7M	507.5M	0.0M
aih-tih-sc-4bcd26-R1_A23YTGFLT4_1	422.3M	0.0M	0.0M	32.0M	2.1M	375.7M	188.0M	187.7M	187.9M	187.8M	229.2M	146.5M	374.3M	0.0M
aih-tih-sc-538ae3-R1_A23YTGFLT4_1	661.9M	0.0M	0.0M	66.1M	3.2M	562.4M	281.3M	281.1M	281.2M	281.2M	297.3M	265.1M	560.7M	0.0M
aih-tih-sc-5519ab-R1_A23YTGFLT4_1	648.7M	0.0M	0.0M	53.5M	3.1M	571.7M	286.1M	285.7M	285.9M	285.8M	347.0M	224.8M	570.1M	0.0M
aih-tih-sc-56cab8-R1_A23YTGFLT4_1	646.9M	0.0M	0.0M	51.6M	3.4M	569.2M	284.8M	284.4M	284.6M	284.6M	308.7M	260.5M	567.5M	0.0M
aih-tih-sc-573e21-R1_A23YTGFLT4_1	644.0M	0.0M	0.0M	49.9M	3.0M	572.8M	286.6M	286.2M	286.5M	286.3M	303.3M	269.5M	571.0M	0.0M
aih-tih-sc-598a93-R1_A23YTGFLT4_1	144.9M	0.0M	0.0M	22.8M	1.2M	113.9M	57.0M	56.9M	57.0M	57.0M	79.4M	34.5M	113.5M	0.0M
aih-tih-sc-5b8b4a-R1_A23YTGFLT4_1	643.5M	0.0M	0.0M	48.0M	3.4M	572.0M	286.2M	285.8M	286.0M	286.0M	325.3M	246.7M	570.1M	0.0M
aih-tih-sc-5ceb9a-R1_A23YTGFLT4_1	610.0M	0.0M	0.0M	37.4M	2.7M	553.5M	276.9M	276.6M	276.8M	276.7M	291.0M	262.5M	551.7M	0.0M
aih-tih-sc-607ec3-R1_A23YTGFLT4_1	434.1M	0.0M	0.0M	65.0M	2.5M	338.7M	169.5M	169.2M	169.3M	169.3M	210.6M	128.0M	337.6M	0.0M
aih-tih-sc-64cd51-R1_A23YTGFLT4_1	642.0M	0.0M	0.0M	46.4M	3.3M	573.0M	286.6M	286.3M	286.5M	286.5M	316.1M	256.9M	571.1M	0.0M
aih-tih-sc-64fc89-R1_A23YTGFLT4_1	640.0M	0.0M	0.0M	43.9M	3.1M	575.2M	287.8M	287.5M	287.7M	287.6M	307.7M	267.6M	573.3M	0.0M
aih-tih-sc-656590-R1_A23YTGFLT4_1	578.7M	0.0M	0.0M	41.4M	3.4M	516.9M	258.6M	258.3M	258.5M	258.4M	285.9M	230.9M	515.3M	0.0M
aih-tih-sc-67e6e5-R1_A23YTGFLT4_1	660.5M	0.0M	0.0M	66.5M	4.1M	567.0M	283.6M	283.3M	283.5M	283.5M	344.9M	222.1M	565.3M	0.0M
aih-tih-sc-69be7e-R1_A23YTGFLT4_1	646.1M	0.0M	0.0M	51.5M	3.4M	571.6M	286.0M	285.6M	285.8M	285.8M	339.2M	232.5M	569.9M	0.0M
aih-tih-sc-6c4b48-R1_A23YTGFLT4_1	635.3M	0.0M	0.0M	39.8M	2.6M	574.3M	287.3M	287.0M	287.2M	287.1M	288.4M	285.9M	572.5M	0.0M
aih-tih-sc-6cc13c-R1_A23YTGFLT4_1	646.8M	0.0M	0.0M	50.5M	3.1M	574.1M	287.2M	286.9M	287.1M	287.0M	318.2M	255.8M	572.1M	0.0M
aih-tih-sc-6d9e6f-R1_A23YTGFLT4_1	636.4M	0.0M	0.0M	41.3M	2.9M	574.6M	287.5M	287.2M	287.3M	287.3M	314.7M	259.9M	572.7M	0.0M
aih-tih-sc-714733-R1_A23YTGFLT4_1	642.2M	0.0M	0.0M	46.3M	3.4M	573.1M	286.7M	286.3M	286.6M	286.5M	304.3M	268.8M	571.1M	0.0M
aih-tih-sc-776623-R1_A23YTGFLT4_1	651.3M	0.0M	0.0M	55.0M	3.4M	572.8M	286.4M	286.4M	286.4M	286.3M	308.9M	263.8M	570.8M	0.0M
aih-tih-sc-790e56-R1_A23YTGFLT4_1	371.9M	0.0M	0.0M	30.9M	2.1M	325.3M	162.7M	162.5M	162.7M	162.6M	189.3M	136.0M	324.2M	0.0M
aih-tih-sc-7f9674-R1_A23YTGFLT4_1	631.5M	0.0M	0.0M	35.7M	2.7M	578.0M	289.2M	288.9M	289.1M	289.0M	318.9M	259.2M	576.1M	0.0M
aih-tih-sc-7fa0fb-R1_A23YTGFLT4_1	408.3M	0.0M	0.0M	33.8M	2.0M	359.2M	179.7M	179.4M	179.6M	179.6M	222.8M	136.4M	358.0M	0.0M
aih-tih-sc-816ec7-R1_A23YTGFLT4_1	641.7M	0.0M	0.0M	46.1M	3.3M	573.5M	286.9M	286.6M	286.8M	286.7M	307.8M	265.7M	571.8M	0.0M
aih-tih-sc-885658-R1_A23YTGFLT4_1	591.1M	0.0M	0.0M	47.7M	3.4M	522.2M	261.3M	260.9M	261.1M	261.1M	310.7M	211.5M	520.6M	0.0M
aih-tih-sc-89f3a3-R1_A23YTGFLT4_1	637.0M	0.0M	0.0M	41.0M	2.9M	575.9M	288.1M	287.8M	288.0M	288.0M	308.3M	267.6M	574.2M	0.0M
aih-tih-sc-8c7069-R1_A23YTGFLT4_1	537.8M	0.0M	0.0M	55.1M	2.8M	461.0M	230.7M	230.3M	230.5M	230.5M	281.8M	179.2M	459.4M	0.0M
aih-tih-sc-8e3550-R1_A23YTGFLT4_1	334.9M	0.0M	0.0M	23.8M	1.7M	299.5M	149.8M	149.6M	149.7M	149.7M	156.3M	143.1M	298.6M	0.0M
aih-tih-sc-92d360-R1_A23YTGFLT4_1	546.5M	0.0M	0.0M	60.3M	2.4M	462.9M	231.6M	231.3M	231.5M	231.4M	278.7M	184.2M	461.3M	0.0M
aih-tih-sc-966ec5-R1_A23YTGFLT4_1	650.4M	0.0M	0.0M	56.5M	3.5M	568.6M	284.5M	284.1M	284.4M	284.3M	339.4M	229.2M	566.6M	0.0M
aih-tih-sc-96d0f6-R1_A23YTGFLT4_1	641.0M	0.0M	0.0M	45.7M	3.0M	572.7M	286.5M	286.2M	286.4M	286.3M	317.8M	254.9M	570.7M	0.0M
aih-tih-sc-9703f5-R1_A23YTGFLT4_1	375.5M	0.0M	0.0M	37.0M	2.3M	323.0M	161.6M	161.4M	161.5M	161.5M	208.3M	114.8M	321.9M	0.0M
aih-tih-sc-992d78-R1_A23YTGFLT4_1	579.8M	0.0M	0.0M	40.0M	2.3M	519.1M	259.7M	259.4M	259.6M	259.5M	293.1M	226.0M	517.2M	0.0M
aih-tih-sc-9b89b4-R1_A23YTGFLT4_1	634.2M	0.0M	0.0M	38.8M	2.8M	576.4M	288.3M	288.0M	288.2M	288.1M	307.1M	269.2M	574.5M	0.0M
aih-tih-sc-9e2647-R1_A23YTGFLT4_1	636.3M	0.0M	0.0M	40.6M	2.5M	575.1M	287.7M	287.4M	287.5M	287.5M	291.6M	283.4M	573.2M	0.0M
aih-tih-sc-a51633-R1_A23YTGFLT4_1	656.7M	0.0M	0.0M	62.0M	4.2M	566.7M	283.5M	283.2M	283.4M	283.3M	317.7M	249.1M	564.9M	0.0M
aih-tih-sc-a558c6-R1_A23YTGFLT4_1	332.8M	0.0M	0.0M	36.3M	2.2M	281.6M	140.9M	140.7M	140.8M	140.8M	190.0M	91.6M	280.7M	0.0M
aih-tih-sc-a8a787-R1_A23YTGFLT4_1	635.5M	0.0M	0.0M	40.2M	2.6M	574.1M	287.1M	286.9M	287.1M	287.0M	285.2M	288.9M	572.4M	0.0M
aih-tih-sc-a97ce1-R1_A23YTGFLT4_1	485.0M	0.0M	0.0M	50.1M	3.4M	413.7M	207.0M	206.6M	206.8M	206.8M	257.8M	155.9M	412.2M	0.0M
aih-tih-sc-a9fe91-R1_A23YTGFLT4_1	662.5M	0.0M	0.0M	67.2M	3.5M	560.7M	280.5M	280.2M	280.4M	280.3M	300.2M	260.5M	559.1M	0.0M
aih-tih-sc-b567d0-R1_A23YTGFLT4_1	493.0M	0.0M	0.0M	30.7M	2.2M	446.9M	223.6M	223.3M	223.5M	223.4M	238.5M	208.4M	445.5M	0.0M
aih-tih-sc-b6a2e8-R1_A23YTGFLT4_1	646.8M	0.0M	0.0M	51.0M	2.9M	571.5M	285.9M	285.6M	285.8M	285.7M	307.4M	264.2M	569.9M	0.0M
aih-tih-sc-b6fd19-R1_A23YTGFLT4_1	639.1M	0.0M	0.0M	43.3M	3.1M	575.2M	287.7M	287.4M	287.6M	287.6M	300.8M	274.4M	573.2M	0.0M
aih-tih-sc-b8457f-R1_A23YTGFLT4_1	652.3M	0.0M	0.0M	56.4M	2.8M	567.5M	283.9M	283.6M	283.8M	283.8M	287.1M	280.5M	565.8M	0.0M
aih-tih-sc-bbc28d-R1_A23YTGFLT4_1	459.5M	0.0M	0.0M	43.1M	2.5M	396.7M	198.5M	198.2M	198.4M	198.3M	243.9M	152.8M	395.0M	0.0M
aih-tih-sc-c35cc3-R1_A23YTGFLT4_1	549.8M	0.0M	0.0M	46.2M	2.9M	483.6M	242.0M	241.6M	241.8M	241.7M	272.5M	211.1M	481.8M	0.0M
aih-tih-sc-c400f1-R1_A23YTGFLT4_1	652.3M	0.0M	0.0M	57.2M	3.5M	568.2M	284.3M	283.9M	284.1M	284.1M	320.5M	247.7M	566.5M	0.0M
aih-tih-sc-c493d4-R1_A23YTGFLT4_1	518.6M	0.0M	0.0M	33.1M	2.8M	470.6M	235.5M	235.1M	235.4M	235.2M	250.2M	220.4M	468.9M	0.0M
aih-tih-sc-cece97-R1_A23YTGFLT4_1	692.7M	0.0M	0.0M	97.3M	4.1M	547.9M	274.2M	273.8M	274.0M	273.9M	335.2M	212.7M	546.1M	0.0M
aih-tih-sc-d1f1a3-R1_A23YTGFLT4_1	639.9M	0.0M	0.0M	43.1M	3.5M	575.7M	288.1M	287.7M	287.9M	287.8M	318.8M	256.9M	573.9M	0.0M
aih-tih-sc-d5fda9-R1_A23YTGFLT4_1	67.5M	0.0M	0.0M	9.5M	0.7M	54.3M	27.2M	27.1M	27.2M	27.2M	40.4M	14.0M	54.1M	0.0M
aih-tih-sc-d666f1-R1_A23YTGFLT4_1	610.6M	0.0M	0.0M	79.5M	4.5M	499.4M	249.9M	249.5M	249.7M	249.7M	326.6M	172.8M	497.7M	0.0M
aih-tih-sc-d963a2-R1_A23YTGFLT4_1	658.2M	0.0M	0.0M	63.5M	3.4M	564.8M	282.5M	282.2M	282.4M	282.4M	311.7M	253.1M	563.1M	0.0M
aih-tih-sc-da06ce-R1_A23YTGFLT4_1	653.8M	0.0M	0.0M	58.6M	4.7M	571.8M	286.0M	285.8M	286.0M	285.8M	293.6M	278.2M	569.8M	0.0M
aih-tih-sc-e08fa3-R1_A23YTGFLT4_1	659.6M	0.0M	0.0M	65.4M	3.7M	557.7M	279.0M	278.7M	278.9M	278.8M	293.8M	263.9M	556.0M	0.0M
aih-tih-sc-e1cae1-R1_A23YTGFLT4_1	388.8M	0.0M	0.0M	36.8M	2.7M	335.5M	167.9M	167.6M	167.8M	167.7M	217.3M	118.2M	334.5M	0.0M
aih-tih-sc-ec2fd6-R1_A23YTGFLT4_1	605.3M	0.0M	0.0M	40.5M	2.8M	544.3M	272.3M	272.0M	272.2M	272.1M	298.0M	246.2M	542.4M	0.0M
aih-tih-sc-ecc0bc-R1_A23YTGFLT4_1	529.1M	0.0M	0.0M	44.5M	3.1M	464.8M	232.6M	232.2M	232.4M	232.3M	280.8M	184.0M	463.2M	0.0M
aih-tih-sc-eecf9f-R1_A23YTGFLT4_1	639.1M	0.0M	0.0M	44.0M	3.9M	575.5M	288.0M	287.4M	287.9M	287.6M	273.0M	302.5M	573.3M	0.0M
aih-tih-sc-f0dbdf-R1_A23YTGFLT4_1	643.2M	0.0M	0.0M	48.0M	3.5M	572.3M	286.3M	285.9M	286.2M	286.1M	313.4M	258.9M	570.4M	0.0M
aih-tih-sc-f26674-R1_A23YTGFLT4_1	630.3M	0.0M	0.0M	34.7M	3.1M	577.6M	289.0M	288.6M	288.9M	288.8M	299.0M	278.6M	575.5M	0.0M
aih-tih-sc-f27606-R1_A23YTGFLT4_1	665.0M	0.0M	0.0M	79.7M	4.7M	551.3M	275.9M	275.4M	275.7M	275.6M	348.8M	202.5M	549.6M	0.0M
aih-tih-sc-f2fa3a-R1_A23YTGFLT4_1	638.1M	0.0M	0.0M	41.5M	3.3M	575.2M	287.8M	287.4M	287.7M	287.5M	316.2M	259.0M	572.9M	0.0M
aih-tih-sc-f6bb6f-R1_A23YTGFLT4_1	636.4M	0.0M	0.0M	40.6M	3.4M	574.6M	287.5M	287.1M	287.4M	287.3M	304.8M	269.8M	572.8M	0.0M
aih-tih-sc-f7ac14-R1_A23YTGFLT4_1	648.1M	0.0M	0.0M	64.3M	4.1M	555.2M	277.8M	277.4M	277.7M	277.6M	360.0M	195.2M	553.4M	0.0M
aih-tih-sc-f834a3-R1_A23YTGFLT4_1	512.8M	0.0M	0.0M	43.3M	2.7M	448.9M	224.7M	224.3M	224.5M	224.5M	287.2M	161.7M	447.5M	0.0M
aih-tih-sc-f8efb2-R1_A23YTGFLT4_1	655.6M	0.0M	0.0M	60.0M	4.1M	568.1M	284.2M	283.9M	284.1M	284.0M	345.2M	222.9M	566.2M	0.0M
aih-tih-sc-fa55c3-R1_A23YTGFLT4_1	644.0M	0.0M	0.0M	47.9M	3.3M	574.2M	287.2M	286.9M	287.1M	287.0M	328.3M	245.9M	572.2M	0.0M
aih-tih-sc-fba77b-R1_A23YTGFLT4_1	128.8M	0.0M	0.0M	22.4M	1.1M	99.0M	49.6M	49.4M	49.5M	49.5M	72.9M	26.1M	98.6M	0.0M
aih-tih-sc-fe8311-R1_A23YTGFLT4_1	634.0M	0.0M	0.0M	38.1M	3.6M	575.8M	288.1M	287.8M	288.0M	287.8M	292.9M	282.9M	573.8M	0.0M
aih-tih-sc-ffabe0-R1_A23YTGFLT4_1	637.3M	0.0M	0.0M	42.4M	3.6M	573.4M	286.9M	286.5M	286.7M	286.6M	316.0M	257.4M	571.5M	0.0M
aih-tih-sc-ffd2af-R1_A23YTGFLT4_1	484.0M	0.0M	0.0M	42.9M	3.4M	421.2M	210.8M	210.4M	210.6M	210.6M	273.6M	147.6M	419.8M	0.0M

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-rseqc_bam_stat

Sort	Column	Description	ID	Scale
\|\|	Total records	Total records	`total_records`	read_count
\|\|	QC failed	QC failed	`qc_failed`	read_count
\|\|	Duplicates	Optical/PCR duplicate	`optical_pcr_duplicate`	read_count
\|\|	Non primary hit	Non primary hit	`non_primary_hits`	read_count
\|\|	Unmapped	Unmapped reads	`unmapped_reads`	read_count
\|\|	Unique	mapq >= mapq_cut (unique)	`mapq_gte_mapq_cut_unique`	read_count
\|\|	Read-1	Read-1	`read_1`	read_count
\|\|	Read-2	Read-2	`read_2`	read_count
\|\|	+ve strand	Reads map to '+'	`reads_map_to_sense`	read_count
\|\|	-ve strand	Reads map to '-'	`reads_map_to_antisense`	read_count
\|\|	Non-splice reads	Non-splice reads	`non-splice_reads`	read_count
\|\|	Splice reads	Splice reads	`splice_reads`	read_count
\|\|	Proper pairs	Reads mapped in proper pairs	`reads_mapped_in_proper_pairs`	read_count
\|\|	Different chrom	Proper-paired reads map to different chrom	`proper-paired_reads_map_to_different_chrom`	read_count

STAR

STAR is an ultrafast universal RNA-seq aligner.DOI: 10.1093/bioinformatics/bts635.

Alignment Scores

Created with MultiQC

Gene Counts

Statistics from results generated using --quantMode GeneCounts. The three tabs show counts for unstranded RNA-seq, counts for the 1st read strand aligned with RNA and counts for the 2nd read strand aligned with RNA.

Created with MultiQC

fastp

fastp An ultra-fast all-in-one FASTQ preprocessor (QC, adapters, trimming, filtering, splitting...).DOI: 10.1093/bioinformatics/bty560.

Filtered Reads

Filtering statistics of sampled reads.

Created with MultiQC

Insert Sizes

Insert size estimation of sampled reads.

Created with MultiQC

Sequence Quality

Average sequencing quality over each base of all reads.

Created with MultiQC

GC Content

Average GC content over each base of all reads.

Created with MultiQC

N content

Average N content over each base of all reads.

Created with MultiQC

FastQC (raw)

FastQC (raw) This section of the report shows FastQC results before adapter trimming.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

Only sequences which first appear in the first 100,000 sequences in each file are analysed. This should be enough to get a good impression for the duplication levels in the whole file. Each sequence is tracked to the end of the file to give a representative count of the overall duplication level.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

The y-axis on the graph shows the quality scores. The higher the score, the better the base call. The background of the graph divides the y axis into very good quality calls (green), calls of reasonable quality (orange), and calls of poor quality (red). The quality of calls on most platforms will degrade as the run progresses, so it is common to see base calls falling into the orange area towards the end of a read.

Created with MultiQC

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

The per sequence quality score report allows you to see if a subset of your sequences have universally low quality values. It is often the case that a subset of sequences will have universally poor quality, however these should represent only a small percentage of the total sequences.

Created with MultiQC

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To enable multiple samples to be shown in a single plot, the base composition data is shown as a heatmap. The colours represent the balance between the four bases: an even distribution should give an even muddy brown colour. Hover over the plot to see the percentage of the four bases under the cursor.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

In a random library you would expect that there would be little to no difference between the different bases of a sequence run, so the lines in this plot should run parallel with each other. The relative amount of each base should reflect the overall amount of these bases in your genome, but in any case they should not be hugely imbalanced from each other.

It's worth noting that some types of library will always produce biased sequence composition, normally at the start of the read. Libraries produced by priming using random hexamers (including nearly all RNA-Seq libraries) and those which were fragmented using transposases inherit an intrinsic bias in the positions at which reads start. This bias does not concern an absolute sequence, but instead provides enrichement of a number of different K-mers at the 5' end of the reads. Whilst this is a true technical bias, it isn't something which can be corrected by trimming and in most cases doesn't seem to adversely affect the downstream analysis.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

In a normal random library you would expect to see a roughly normal distribution of GC content where the central peak corresponds to the overall GC content of the underlying genome. Since we don't know the the GC content of the genome the modal GC content is calculated from the observed data and used to build a reference distribution.

An unusually shaped distribution could indicate a contaminated library or some other kinds of biased subset. A normal distribution which is shifted indicates some systematic bias which is independent of base position. If there is a systematic bias which creates a shifted normal distribution then this won't be flagged as an error by the module since it doesn't know what your genome's GC content should be.

Created with MultiQC

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

If a sequencer is unable to make a base call with sufficient confidence then it will normally substitute an N rather than a conventional base call. This graph shows the percentage of base calls at each position for which an N was called.

It's not unusual to see a very low proportion of Ns appearing in a sequence, especially nearer the end of a sequence. However, if this proportion rises above a few percent it suggests that the analysis pipeline was unable to interpret the data well enough to make valid base calls.

Created with MultiQC

Sequence Length Distribution

The distribution of fragment sizes (read lengths) found. See the FastQC help

Created with MultiQC

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

In a diverse library most sequences will occur only once in the final set. A low level of duplication may indicate a very high level of coverage of the target sequence, but a high level of duplication is more likely to indicate some kind of enrichment bias (eg PCR over amplification). This graph shows the degree of duplication for every sequence in a library: the relative number of sequences with different degrees of duplication.

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

In a properly diverse library most sequences should fall into the far left of the plot in both the red and blue lines. A general level of enrichment, indicating broad oversequencing in the library will tend to flatten the lines, lowering the low end and generally raising other categories. More specific enrichments of subsets, or the presence of low complexity contaminants will tend to produce spikes towards the right of the plot.

Created with MultiQC

Overrepresented sequences by sample

The total amount of overrepresented sequences found in each library.

FastQC calculates and lists overrepresented sequences in FastQ files. It would not be possible to show this for all samples in a MultiQC report, so instead this plot shows the number of sequences categorized as overrepresented.

Sometimes, a single sequence may account for a large number of reads in a dataset. To show this, the bars are split into two: the first shows the overrepresented reads that come from the single most common sequence. The second shows the total count from all remaining overrepresented sequences.

From the FastQC Help:

A normal high-throughput library will contain a diverse set of sequences, with no individual sequence making up a tiny fraction of the whole. Finding that a single sequence is very overrepresented in the set either means that it is highly biologically significant, or indicates that the library is contaminated, or not as diverse as you expected.

FastQC lists all the sequences which make up more than 0.1% of the total. To conserve memory only sequences which appear in the first 100,000 sequences are tracked to the end of the file. It is therefore possible that a sequence which is overrepresented but doesn't appear at the start of the file for some reason could be missed by this module.

Created with MultiQC

Top overrepresented sequences

Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

Showing ²⁰/₂₀ rows and ³/₃ columns.

Overrepresented sequence	Samples	Occurrences	% of all reads
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	192	198583646	0.1850%
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	4	1852	0.0000%
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	4	1734	0.0000%
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG	4	883	0.0000%
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT	3	466	0.0000%
GGGGGAGAGAGAGAGAGAGAGGGCGC	2	12	0.0000%
GCGCCCTCTCTCTCTCTCTCTCCCCC	2	12	0.0000%
GATCGGAAGAGCACACGTCTGAACTCAGTCACCAAGACTCAAATCTGGGG	2	201	0.0000%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTCATCCAATCTGGG	1	371372	0.0003%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACTCATCCAATCTAGG	1	60962	0.0001%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGAATAGAATCTGGG	1	132195	0.0001%
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACCGAATAGAATCTAGG	1	53724	0.0001%
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC	1	68	0.0000%
GACTGGAGTTCAGACGTGTGCTCTCCGATC	1	22	0.0000%
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC	1	21	0.0000%
GACTGGAGTTCAGACGTGTGCTCTTCCGATC	1	20	0.0000%
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	1	19	0.0000%
GCGGGGGAGAGAGAGAGAGAGAGGGCGC	1	13	0.0000%
TCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	12	0.0000%
TCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	11	0.0000%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-fastqc_top_overrepresented_sequences_table

Sort	Group	Column	Description	ID	Scale
\|\|	FastQC (raw)	Samples	Number of samples where this sequence is overrepresented	`samples`	None
\|\|	FastQC (raw)	Occurrences	Total number of occurrences of the sequence (among the samples where the sequence is overrepresented)	`total_count`	None
\|\|	FastQC (raw)	% of all reads	Total number of occurrences as the percentage of all reads (among samples where the sequence is overrepresented)	`total_percent`	None

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

The plot shows a cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position. Once a sequence has been seen in a read it is counted as being present right through to the end of the read so the percentages you see will only increase as the read length goes on.

Created with MultiQC

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

FastQC assigns a status for each section of the report. These give a quick evaluation of whether the results of the analysis seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

It is important to stress that although the analysis results appear to give a pass/fail result, these evaluations must be taken in the context of what you expect from your library. A 'normal' sample as far as FastQC is concerned is random and diverse. Some experiments may be expected to produce libraries which are biased in particular ways. You should treat the summary evaluations therefore as pointers to where you should concentrate your attention and understand why your library may not look random and diverse.

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

In this heatmap, we summarise all of these into a single heatmap for a quick overview. Note that not all FastQC sections have plots in MultiQC reports, but all status checks are shown in this heatmap.

Min:

Max:

Created with MultiQC

FastQC (trimmed)

FastQC (trimmed) This section of the report shows FastQC results after adapter trimming.

Sequence Counts

Sequence counts for each sample. Duplicate read counts are an estimate only.

This plot show the total number of reads, broken down into unique and duplicate if possible (only more recent versions of FastQC give duplicate info).

You can read more about duplicate calculation in the FastQC documentation. A small part has been copied here for convenience:

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Sequence Quality Histograms

The mean quality value across each base position in the read.

To enable multiple samples to be plotted on the same graph, only the mean quality scores are plotted (unlike the box plots seen in FastQC reports).

Taken from the FastQC help:

Created with MultiQC

Per Sequence Quality Scores

The number of reads with average quality scores. Shows if a subset of reads has poor quality.

From the FastQC help:

Created with MultiQC

Per Base Sequence Content

The proportion of each base position for which each of the four normal DNA bases has been called.

To see the data as a line plot, as in the original FastQC graph, click on a sample track.

From the FastQC help:

Per Base Sequence Content plots out the proportion of each base position in a file for which each of the four normal DNA bases has been called.

Click a sample row to see a line plot for that dataset.

Rollover for sample name

Position: -

%T: -

%C: -

%A: -

%G: -

Per Sequence GC Content

The average GC content of reads. Normal random library typically have a roughly normal distribution of GC content.

From the FastQC help:

This module measures the GC content across the whole length of each sequence in a file and compares it to a modelled normal distribution of GC content.

Created with MultiQC

Per Base N Content

The percentage of base calls at each position for which an N was called.

From the FastQC help:

Created with MultiQC

Sequence Length Distribution

The distribution of fragment sizes (read lengths) found. See the FastQC help

Created with MultiQC

Sequence Duplication Levels

The relative level of duplication found for every sequence.

From the FastQC Help:

The duplication detection requires an exact sequence match over the whole length of the sequence. Any reads over 75bp in length are truncated to 50bp for this analysis.

Created with MultiQC

Overrepresented sequences by sample

The total amount of overrepresented sequences found in each library.

From the FastQC Help:

Created with MultiQC

Top overrepresented sequences

Top overrepresented sequences across all samples. The table shows 20 most overrepresented sequences across all samples, ranked by the number of samples they occur in.

Showing ²⁰/₂₀ rows and ³/₃ columns.

Overrepresented sequence	Samples	Occurrences	% of all reads
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG	96	81076918	0.1659%
TCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	2	926	0.0000%
GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA	2	862	0.0000%
AGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAG	2	437	0.0000%
GGGGGAGAGAGAGAGAGAGAGGGCGC	2	12	0.0000%
GCGCCCTCTCTCTCTCTCTCTCCCCC	2	12	0.0000%
CTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCT	2	234	0.0000%
GCTGCCGAGTGTTACAGCCCCCCCGGCAGC	1	68	0.0000%
GACTGGAGTTCAGACGTGTGCTCTCCGATC	1	22	0.0000%
CTTCGCGCCCTCTCTCTCTCTCTCTCCCCC	1	21	0.0000%
GACTGGAGTTCAGACGTGTGCTCTTCCGATC	1	20	0.0000%
GCTGCCCCCGAGTGTTACAGCCCCCCCGGCAGCAGC	1	19	0.0000%
GCGGGGGAGAGAGAGAGAGAGAGGGCGC	1	13	0.0000%
TCTCTCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	12	0.0000%
TCTCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	11	0.0000%
TCTCTTTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTC	1	8	0.0000%
TCTCGATCCACGTCCCGGGAACGGTGCCGGGACTCCCTGTGTCTGTGGTC	1	8	0.0000%
GTGCCAATGGTGCTCCTGGTATTGCTGGTGCTCCTGGCTTCCCTGGTGCC	1	7	0.0000%
GCGGGGGAGAGAGAGAGAGAGAGGGCGCG	1	7	0.0000%
TCTAGATGAGCAGCTGTCCCAGGTCCGAGAGGCCGACCGGCACCCGGGCA	1	6	0.0000%

Uncheck the tick box to hide columns. Click and drag the handle on the left to change order. Table ID: table-fastqc_top_overrepresented_sequences_table

Sort	Group	Column	Description	ID	Scale
\|\|	FastQC (trimmed)	Samples	Number of samples where this sequence is overrepresented	`samples`	None
\|\|	FastQC (trimmed)	Occurrences	Total number of occurrences of the sequence (among the samples where the sequence is overrepresented)	`total_count`	None
\|\|	FastQC (trimmed)	% of all reads	Total number of occurrences as the percentage of all reads (among samples where the sequence is overrepresented)	`total_percent`	None

Adapter Content

The cumulative percentage count of the proportion of your library which has seen each of the adapter sequences at each position.

Note that only samples with ≥ 0.1% adapter contamination are shown.

There may be several lines per sample, as one is shown for each adapter detected in the file.

From the FastQC Help:

Created with MultiQC

Status Checks

Status for each FastQC section showing whether results seem entirely normal (green), slightly abnormal (orange) or very unusual (red).

Specific guidance on how to interpret the output of each module can be found in the relevant report section, or in the FastQC help.

Min:

Max:

Created with MultiQC

Software Versions

Software Versions lists versions of software tools extracted from file contents.

Group	Software	Version
AGAT_CONVERTSPGFF2TSV	agat	`1.2.0`
ARRIBA	arriba	`2.4.0`
ARRIBA_FILTER	filter_arriba_main.py	`1.0`
	pandas	`3.0.0`
	pyyaml	`6.0.3`
ARRIBA_VISUALISATION	arriba	`2.4.0`
CANCER_INTRONS_TO_VCF	python	`3.11.0`
CONTAMINATION_SKIPPED_LOW_READS	contamination_skipped_low_reads	`1.0.0`
CTATSPLICING_STARTOCANCERINTRONS	ctat-splicing	`0.0.3`
EXTRACT_VAF_FEATURES	extract_vaf_features	`1.0.0`
	python	`3.11.14`
FASTQC	fastqc	`0.12.1`
FASTQC_FOR_FASTP	fastqc	`0.12.1`
FUSIONINSPECTOR	STAR-Fusion	`1.12.0`
FUSIONREPORT	fusion_report	`2.1.5`
	fusion_report DB retrieval	`null`
GATK4_MARKDUPLICATES	gatk4	`4.4.0.0`
	samtools	`1.17`
GTF2BED	perl	`5.26.2`
KALLISTO_QUANT	kallisto	`0.51.1`
KALLISTO_TO_TX2GENE_QC	expression_qc_and_signatures	`1.0.0`
	python	`3.11.0`
NGSCheckMATE	ngscheckmate	`1.0.0`
PREDICT_CONTAMINATION	predict_contamination	`1.0.0`
	python	`3.11.14`
PRESEQ_LCEXTRAP	preseq	`3.2.0`
PRESEQ_TO_MULTIQC	preseq_to_multiqc	`1.0.0`
	python	`3.11.14`
RSEQC_BAMSTAT	rseqc	`5.0.2`
RSEQC_INFEREXPERIMENT	rseqc	`5.0.2`
RSEQC_INNERDISTANCE	rseqc	`5.0.2`
RSEQC_JUNCTIONANNOTATION	rseqc	`5.0.2`
RSEQC_JUNCTIONSATURATION	rseqc	`5.0.2`
RSEQC_READDISTRIBUTION	rseqc	`5.0.2`
RSEQC_READDUPLICATION	rseqc	`5.0.2`
RSEQC_TO_MULTIQC	python	`3.11.14`
	rseqc_to_multiqc	`1.0.0`
SAMTOOLS_INDEX_ALIGNMENT_CRAM	samtools	`1.17`
SAMTOOLS_INDEX_STAR_ALIGNMENT	samtools	`1.17`
SAMTOOLS_VIEW_ALIGNMENT_CRAM	samtools	`1.17`
SEQTOOL_TO_MULTIQC	python	`3.11.14`
	seqtool_to_multiqc	`1.0.0`
STARFUSION	STAR-Fusion	`1.12.0`
STARFUSION_FILTER	boto3	`unknown`
	filter_starfusion_main.py	`1.0`
	numpy	`2.4.1`
	pandas	`3.0.0`
	pyyaml	`6.0.3`
STAR_ALIGNMENT	gawk	`5.1.0`
	samtools	`1.16.1`
	star	`2.7.10a0`
VCF_COLLECT	HGNC DB retrieval	`2025-01-22/23:32`
	python	`3.11.14`
Workflow	Nextflow	`25.10.2`
	nf-core/rnafusion	`3.0.2`
fastp	fastp	`0.23.4`

nf-core/rnafusion Methods Description

Suggested text and references to use when describing pipeline usage within the methods section of a publication.

Methods

Data was processed using nf-core/rnafusion v3.0.2 of the nf-core collection of workflows (Ewels et al., 2020), utilising reproducible software environments from the Bioconda (Grüning et al., 2018) and Biocontainers (da Veiga Leprevost et al., 2017) projects.

The pipeline was executed with Nextflow v25.10.2 (Di Tommaso et al., 2017) with the following command:

nextflow run /code/main.nf -output-dir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0021/A23YTGFLT4/nfcore_rnafusion__73d51683--20260607-225254' --project-dir /Users/anaik/Documents/Altera/rnafusion -profile docker,eks,std_run -name dev_rna_exp0021-73d51683 --rundir 's3://natera-labdata-rnd-dev-s3/illumina/20260529_LH00995_0048_A23YTGFLT4/' --bclconvert_samplesheet 's3://natera-labdata-rnd-dev-s3/illumina/20260529_LH00995_0048_A23YTGFLT4/bclconvert_samplesheet.csv' --flowcell_id 20260529_LH00995_0048_A23YTGFLT4 --outdir 's3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0021/A23YTGFLT4/nfcore_rnafusion__73d51683--20260607-225254'

References

Di Tommaso, P., Chatzou, M., Floden, E. W., Barja, P. P., Palumbo, E., & Notredame, C. (2017). Nextflow enables reproducible computational workflows. Nature Biotechnology, 35(4), 316-319. doi: 10.1038/nbt.3820
Ewels, P. A., Peltzer, A., Fillinger, S., Patel, H., Alneberg, J., Wilm, A., Garcia, M. U., Di Tommaso, P., & Nahnsen, S. (2020). The nf-core framework for community-curated bioinformatics pipelines. Nature Biotechnology, 38(3), 276-278. doi: 10.1038/s41587-020-0439-x
Grüning, B., Dale, R., Sjödin, A., Chapman, B. A., Rowe, J., Tomkins-Tinch, C. H., Valieris, R., Köster, J., & Bioconda Team. (2018). Bioconda: sustainable and comprehensive software distribution for the life sciences. Nature Methods, 15(7), 475–476. doi: 10.1038/s41592-018-0046-7
da Veiga Leprevost, F., Grüning, B. A., Alves Aflitos, S., Röst, H. L., Uszkoreit, J., Barsnes, H., Vaudel, M., Moreno, P., Gatto, L., Weber, J., Bai, M., Jimenez, R. C., Sachsenberg, T., Pfeuffer, J., Vera Alvarez, R., Griss, J., Nesvizhskii, A. I., & Perez-Riverol, Y. (2017). BioContainers: an open-source and community-driven framework for software standardization. Bioinformatics (Oxford, England), 33(16), 2580–2582. doi: 10.1093/bioinformatics/btx192

Notes:

If available, make sure to update the text to include the Zenodo DOI of version of the pipeline used.
The command above does not include parameters contained in any configs or profiles that may have been used. Ensure the config file is also uploaded with your publication!
You should also cite all software used within this run. Check the "Software Versions" of this report to get version information.

nf-core/rnafusion Workflow Summary

- this information is collected when the pipeline is started.

Core Nextflow options

runName: dev_rna_exp0021-73d51683
containerEngine: docker
launchDir: /code
workDir: /natera-rnd-pltf-dev-nextflow-scratch-01/work
projectDir: /code
userName: nextflow
profile: docker,eks,std_run
configFiles: N/A

Skip steps

skip_picard_qc: true

Input/output options

outdir: s3://natera-platform-sandbox/pipeline-outputs/cgp_runs/nextflow/dev_rna_exp0021/A23YTGFLT4/nfcore_rnafusion__73d51683--20260607-225254
rundir: s3://natera-labdata-rnd-dev-s3/illumina/20260529_LH00995_0048_A23YTGFLT4/
flowcell_id: 20260529_LH00995_0048_A23YTGFLT4
strand: reverse
bclconvert_samplesheet: s3://natera-labdata-rnd-dev-s3/illumina/20260529_LH00995_0048_A23YTGFLT4/bclconvert_samplesheet.csv
infer_sample_type_map: true
sample_type_map_positive_substring: HD789
genomes_base: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38
read_length: 150
arriba: true
arriba_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/
arriba_ref_blacklist: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/blacklist_hg38_GRCh38_v2.4.0.tsv.gz
arriba_ref_cytobands: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/cytobands_hg38_GRCh38_v2.4.0.tsv
arriba_ref_known_fusions: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/known_fusions_hg38_GRCh38_v2.4.0.tsv.gz
arriba_ref_protein_domains: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/arriba/protein_domains_hg38_GRCh38_v2.4.0.gff3
arriba_gtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir//ref_annot.gtf
ensembl_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/
fusioncatcher_limitSjdbInsertNsj: 2000000
fusioncatcher_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusioncatcher/human_v102/
fusioninspector_limitSjdbInsertNsj: 1000000
fusionreport_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/fusion_report_db/
hgnc_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/hgnc_complete_set.txt
hgnc_date: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/hgnc/HGNC-DB-timestamp.txt
starfusion: true
starfusion_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/starfusion/ctat_genome_lib_build_dir/
starindex_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
starindex_ensembl_ref: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/star/
tools_cutoff: 1

Read trimming options

fastp_trim: true
fastp_reads_to_process: 300000000
adapter_fasta: []

Alignment compression options

cram: starfusion,arriba

Reference genome options

genome: GRCh38
fasta: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa
fai: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.all.fa.fai
gtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.gtf
chrgtf: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf
transcript: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.cdna.all.fa.gz
refflat: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.chr.gtf.refflat
rrna_intervals: s3://natera-platform-sandbox/pipeline-resources/AIH/rna/GRCh38/ensembl/Homo_sapiens.GRCh38.102.interval_list

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FastQC: Top overrepresented sequences: Columns

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Software Versions

nf-core/rnafusion Methods Description

Methods

References

Notes:

nf-core/rnafusion Workflow Summary

v1.21

Highlight Samples

Rename Samples

Show / Hide Samples

Mark Duplicates

Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

Per Sequence GC Content

Per Base N Content

Sequence Duplication Levels

Overrepresented sequences by sample

Adapter Content

Status Checks

Sequence Counts

Sequence Quality Histograms

Per Sequence Quality Scores

Per Base Sequence Content

Per Sequence GC Content

Per Base N Content

Sequence Duplication Levels

Overrepresented sequences by sample

Adapter Content

Status Checks