[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-01eb49-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
Reading reference bed file:  ref_annot.bed  ...  Done
Load BAM file ...  Done

===================================================================
Total splicing  Events:	326074209
Known Splicing Events:	322852786
Partial Novel Splicing Events:	1727509
Novel Splicing Events:	1286433
Filtered Splicing Events:	207481

Total splicing  Junctions:	612914
Known Splicing Junctions:	292070
Partial Novel Splicing Junctions:	190044
Novel Splicing Junctions:	130800

===================================================================
Create BED file ...
Create Interact file ...