+ mkdir aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1 + all_qc -bam aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bam -beds Twist_rna_exome_AND_fusion_hg38.bed -dbsnp_vcf Homo_sapiens_assembly38.dbsnp138.vcf.gz -contamination_bed dbsnp_hg38_contam_sites.bed -output_folder aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1 -reference ref_genome.fa -region chr20 [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [mpileup] 1 samples in 1 input files [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources /opt/conda/lib/python3.9/site-packages/pyranges/__init__.py:8: UserWarning: pkg_resources is deprecated as an API. See https://setuptools.pypa.io/en/latest/pkg_resources.html. The pkg_resources package is slated for removal as early as 2025-11-30. Refrain from using this package or pin to Setuptools<81. import pkg_resources [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai 0it [00:00, ?it/s] 139696it [00:00, 1396878.07it/s] 289600it [00:00, 1456944.08it/s] 452662it [00:00, 1536221.88it/s] 611587it [00:00, 1557137.82it/s] 778760it [00:00, 1598440.96it/s] 946162it [00:00, 1624111.50it/s] 1108574it [00:00, 1623652.87it/s] 1275533it [00:00, 1638257.07it/s] 1443016it [00:00, 1649668.17it/s] 1611628it [00:01, 1660903.69it/s] 1777719it [00:01, 1658757.89it/s] 1944633it [00:01, 1661895.62it/s] 2110824it [00:01, 1642881.82it/s] 2278617it [00:01, 1653362.19it/s] 2451572it [00:01, 1676042.94it/s] 2625122it [00:01, 1693847.41it/s] 2794545it [00:01, 1689685.84it/s] 2963541it [00:01, 1686269.99it/s] 3132854it [00:01, 1688304.24it/s] 3301699it [00:02, 1673691.91it/s] 3469104it [00:02, 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[00:07, 1688931.45it/s] 13425208it [00:08, 1682274.88it/s] 13498098it [00:08, 1667694.80it/s] [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: Homo_sapiens_assembly38.dbsnp138.vcf.gz.tbi [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai [W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md.bai + merge_qc -folders aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1/aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1.md -output aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1/aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_full_basic_stats.tsv + head -n1 aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1/aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_full_basic_stats.tsv ++ basename Twist_rna_exome_AND_fusion_hg38.bed + grep -e Twist_rna_exome_AND_fusion_hg38.bed aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1/aih-tih-sc-2e5fe9-R1_A23YTGFLT4_1_full_basic_stats.tsv