[W::hts_idx_load3] The index file is older than the data file: aih-tih-sc-3b436b-R1_A23YTGFLT4_1.Aligned.sortedByCoord.out.bam.bai
reading reference bed file:  ref_annot.bed  ...  Done! Total 386135 known splicing junctions.
Load BAM file ...  Done
shuffling alignments ... Done
sampling 5% (7339516) splicing reads. 227575 splicing junctions. 176258 known splicing junctions. 51317 novel splicing junctions.
sampling 10% (14679033) splicing reads. 276531 splicing junctions. 190411 known splicing junctions. 86120 novel splicing junctions.
sampling 15% (22018549) splicing reads. 312185 splicing junctions. 198305 known splicing junctions. 113880 novel splicing junctions.
sampling 20% (29358066) splicing reads. 341451 splicing junctions. 203842 known splicing junctions. 137609 novel splicing junctions.
sampling 25% (36697583) splicing reads. 365385 splicing junctions. 207728 known splicing junctions. 157657 novel splicing junctions.
sampling 30% (44037099) splicing reads. 386258 splicing junctions. 210772 known splicing junctions. 175486 novel splicing junctions.
sampling 35% (51376616) splicing reads. 404362 splicing junctions. 213179 known splicing junctions. 191183 novel splicing junctions.
sampling 40% (58716133) splicing reads. 420413 splicing junctions. 215281 known splicing junctions. 205132 novel splicing junctions.
sampling 45% (66055649) splicing reads. 434574 splicing junctions. 216984 known splicing junctions. 217590 novel splicing junctions.
sampling 50% (73395166) splicing reads. 447237 splicing junctions. 218491 known splicing junctions. 228746 novel splicing junctions.
sampling 55% (80734683) splicing reads. 458471 splicing junctions. 219737 known splicing junctions. 238734 novel splicing junctions.
sampling 60% (88074199) splicing reads. 468698 splicing junctions. 220859 known splicing junctions. 247839 novel splicing junctions.
sampling 65% (95413716) splicing reads. 477849 splicing junctions. 221742 known splicing junctions. 256107 novel splicing junctions.
sampling 70% (102753233) splicing reads. 485877 splicing junctions. 222546 known splicing junctions. 263331 novel splicing junctions.
sampling 75% (110092749) splicing reads. 493180 splicing junctions. 223269 known splicing junctions. 269911 novel splicing junctions.
sampling 80% (117432266) splicing reads. 499468 splicing junctions. 223893 known splicing junctions. 275575 novel splicing junctions.
sampling 85% (124771783) splicing reads. 504948 splicing junctions. 224414 known splicing junctions. 280534 novel splicing junctions.
sampling 90% (132111299) splicing reads. 509787 splicing junctions. 224845 known splicing junctions. 284942 novel splicing junctions.
sampling 95% (139450816) splicing reads. 513796 splicing junctions. 225222 known splicing junctions. 288574 novel splicing junctions.
sampling 100% (146790333) splicing reads. 517158 splicing junctions. 225537 known splicing junctions. 291621 novel splicing junctions.